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The map label for this gene is rimO

Identifier: 114331380

GI number: 114331380

Start: 1460630

End: 1461967

Strand: Direct

Name: rimO

Synonym: Neut_1389

Alternate gene names: 114331380

Gene position: 1460630-1461967 (Clockwise)

Preceding gene: 114331379

Following gene: 114331381

Centisome position: 54.89

GC content: 52.99

Gene sequence:

>1338_bases
ATGACAGCTGCCTCATCCCTACACCCCCCTGCAATTCCACGCGTTGGTTTTGTATCACTTGGCTGCCCCAAGGCAACGGT
TGATTCCGAACGTATCCTCACCTGCCTGCGAGCAGAGGGCTACCTGATCTCACCCAACTACGCCGATGCTGATCTGGTGG
TCGTCAACACCTGCGGTTTTATTGATAGTGCCGTGGCGGAATCGCTGGAAACCATTGGCGAAGCACTGACTGAAAATGGC
AAGGTGATTGTAACCGGCTGCCTGGGTGCAAAAGAGGATGTCATCCGGCAGGCGCACCCCAGCGTGCTGGCAGTAACCGG
CCCGCAGGCAACCGAGGAAGTGATGGCCGCCATTCACCATCATTTACCCAAACCGCACGATCCTTATCTTGATCTGGTAC
CGCCACAGGGAATCAAGCTCACGCCGAAGCATTACGCCTATCTCAAAATTTCCGAAGGCTGCAATCATCATTGCACGTTC
TGCATTATTCCCAGCATGCGCGGTGATCTGGTCAGTCGACCGGTGGGTGGTGTACTGCAGGAGGCGCAAAGTCTGGTCGA
AGCTGGTGTCAAGGAATTGCTGATTATTTCCCAGGATACCAGTGCTTACGGGGTGGATATCAAATATCGCACCGGCTTCT
GGCAAGGCAGACCCGTTCGCACTCGCATCACCGAACTGGCGAACGCACTGGGGGAATTCGGTATCTGGGTACGGCTGCAC
TATGTCTATCCCTATCCACATGTGGATGAACTGATCCCATTAATGGCAGAAGGCAAGCTCCTGCCTTATCTGGACATTCC
TTTTCAGCACGCCAACAAACGCATACTCAAGCTGATGAAACGCCCAGCCAATAGTGAAAACGTACTGGTGCGCATTCAGC
AGTGGCGCAAAACCTGCCCGGACATTGCTCTGCGCAGCACTTTTATTGTTGGTTTTCCGGGAGAAACCGAAGCAGAATTC
GAGGAACTGCTCGCTTTTCTGGAAGAAGCACAACTGGATCGGGTGGGGGCATTTACCTATTCGCCGGTCAAAGGCGCGGC
TGCCAATGTATTGCCTGATCATGTTCCTCCCGAAATCCAGCAAGAACGACTGGAAAGACTGATGCAACAGCAGGAAATCA
TTAGCAAACAACGCCTGGCTGTGAAAAAAGGCCAGCAGCTCAGGGTGCTGGTGGACACAGTTGATGAAGAAGGCGCCATC
GCCAGAAGTTATGCCGATGCACCGGAAATTGACGGCGTTGTTTACATTAATGATGCCTCAACCCTTAAACCGGGTGACTG
GGCAGATGTGCGCGTTACCGATACTGACATTCACGACCTGTGGACAGAGAAAATCTAA

Upstream 100 bases:

>100_bases
AAATCTTGTGCAATATATTCCCCGGTGGCACCATACCGCCTTTCATCCGGAGCCAGGAAAGCGGTGATGGATATAAACCA
TTGTATATACGGAAATCTGA

Downstream 100 bases:

>100_bases
CGCGAACAGTAAACTCCTGAATTGCTTCGGCGACTGTGCCTGCCCGCAAAAGTGGCATTTGTATCATTGCCACTTTTTCC
TCAATCCTTACAAGGTGCTG

Product: ribosomal protein S12 methylthiotransferase

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTAASSLHPPAIPRVGFVSLGCPKATVDSERILTCLRAEGYLISPNYADADLVVVNTCGFIDSAVAESLETIGEALTENG
KVIVTGCLGAKEDVIRQAHPSVLAVTGPQATEEVMAAIHHHLPKPHDPYLDLVPPQGIKLTPKHYAYLKISEGCNHHCTF
CIIPSMRGDLVSRPVGGVLQEAQSLVEAGVKELLIISQDTSAYGVDIKYRTGFWQGRPVRTRITELANALGEFGIWVRLH
YVYPYPHVDELIPLMAEGKLLPYLDIPFQHANKRILKLMKRPANSENVLVRIQQWRKTCPDIALRSTFIVGFPGETEAEF
EELLAFLEEAQLDRVGAFTYSPVKGAAANVLPDHVPPEIQQERLERLMQQQEIISKQRLAVKKGQQLRVLVDTVDEEGAI
ARSYADAPEIDGVVYINDASTLKPGDWADVRVTDTDIHDLWTEKI

Sequences:

>Translated_445_residues
MTAASSLHPPAIPRVGFVSLGCPKATVDSERILTCLRAEGYLISPNYADADLVVVNTCGFIDSAVAESLETIGEALTENG
KVIVTGCLGAKEDVIRQAHPSVLAVTGPQATEEVMAAIHHHLPKPHDPYLDLVPPQGIKLTPKHYAYLKISEGCNHHCTF
CIIPSMRGDLVSRPVGGVLQEAQSLVEAGVKELLIISQDTSAYGVDIKYRTGFWQGRPVRTRITELANALGEFGIWVRLH
YVYPYPHVDELIPLMAEGKLLPYLDIPFQHANKRILKLMKRPANSENVLVRIQQWRKTCPDIALRSTFIVGFPGETEAEF
EELLAFLEEAQLDRVGAFTYSPVKGAAANVLPDHVPPEIQQERLERLMQQQEIISKQRLAVKKGQQLRVLVDTVDEEGAI
ARSYADAPEIDGVVYINDASTLKPGDWADVRVTDTDIHDLWTEKI
>Mature_444_residues
TAASSLHPPAIPRVGFVSLGCPKATVDSERILTCLRAEGYLISPNYADADLVVVNTCGFIDSAVAESLETIGEALTENGK
VIVTGCLGAKEDVIRQAHPSVLAVTGPQATEEVMAAIHHHLPKPHDPYLDLVPPQGIKLTPKHYAYLKISEGCNHHCTFC
IIPSMRGDLVSRPVGGVLQEAQSLVEAGVKELLIISQDTSAYGVDIKYRTGFWQGRPVRTRITELANALGEFGIWVRLHY
VYPYPHVDELIPLMAEGKLLPYLDIPFQHANKRILKLMKRPANSENVLVRIQQWRKTCPDIALRSTFIVGFPGETEAEFE
ELLAFLEEAQLDRVGAFTYSPVKGAAANVLPDHVPPEIQQERLERLMQQQEIISKQRLAVKKGQQLRVLVDTVDEEGAIA
RSYADAPEIDGVVYINDASTLKPGDWADVRVTDTDIHDLWTEKI

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI93277076, Length=408, Percent_Identity=25.7352941176471, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI28872784, Length=475, Percent_Identity=24.6315789473684, Blast_Score=132, Evalue=8e-31,
Organism=Homo sapiens, GI28872782, Length=475, Percent_Identity=24.8421052631579, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1787057, Length=434, Percent_Identity=67.5115207373272, Blast_Score=628, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=434, Percent_Identity=29.0322580645161, Blast_Score=149, Evalue=5e-37,
Organism=Caenorhabditis elegans, GI17553146, Length=474, Percent_Identity=24.6835443037975, Blast_Score=118, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=294, Percent_Identity=27.2108843537415, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=25.5765199161426, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI19922432, Length=384, Percent_Identity=25.78125, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_NITEC (Q0AG95)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747602.1
- ProteinModelPortal:   Q0AG95
- SMR:   Q0AG95
- STRING:   Q0AG95
- GeneID:   4273996
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1389
- NMPDR:   fig|335283.3.peg.586
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q0AG95
- ProtClustDB:   PRK14862
- BioCyc:   NEUT335283:NEUT_1389-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49187; Mature: 49056

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAASSLHPPAIPRVGFVSLGCPKATVDSERILTCLRAEGYLISPNYADADLVVVNTCGF
CCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCEEEEEECCH
IDSAVAESLETIGEALTENGKVIVTGCLGAKEDVIRQAHPSVLAVTGPQATEEVMAAIHH
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
HLPKPHDPYLDLVPPQGIKLTPKHYAYLKISEGCNHHCTFCIIPSMRGDLVSRPVGGVLQ
HCCCCCCCCEECCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHH
EAQSLVEAGVKELLIISQDTSAYGVDIKYRTGFWQGRPVRTRITELANALGEFGIWVRLH
HHHHHHHHCHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
YVYPYPHVDELIPLMAEGKLLPYLDIPFQHANKRILKLMKRPANSENVLVRIQQWRKTCP
EEECCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCC
DIALRSTFIVGFPGETEAEFEELLAFLEEAQLDRVGAFTYSPVKGAAANVLPDHVPPEIQ
CCEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHCCCCCCCCHHHH
QERLERLMQQQEIISKQRLAVKKGQQLRVLVDTVDEEGAIARSYADAPEIDGVVYINDAS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHCCCCCCCCCEEEECCCC
TLKPGDWADVRVTDTDIHDLWTEKI
CCCCCCCCEEEEECCHHHHHHHCCC
>Mature Secondary Structure 
TAASSLHPPAIPRVGFVSLGCPKATVDSERILTCLRAEGYLISPNYADADLVVVNTCGF
CCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEECCCCCCCCEEEEEECCH
IDSAVAESLETIGEALTENGKVIVTGCLGAKEDVIRQAHPSVLAVTGPQATEEVMAAIHH
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCEEEEECCCHHHHHHHHHHH
HLPKPHDPYLDLVPPQGIKLTPKHYAYLKISEGCNHHCTFCIIPSMRGDLVSRPVGGVLQ
HCCCCCCCCEECCCCCCCEECCCCEEEEEECCCCCCCEEEEEECCCCCHHHHCCHHHHHH
EAQSLVEAGVKELLIISQDTSAYGVDIKYRTGFWQGRPVRTRITELANALGEFGIWVRLH
HHHHHHHHCHHHEEEEECCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCEEEEEE
YVYPYPHVDELIPLMAEGKLLPYLDIPFQHANKRILKLMKRPANSENVLVRIQQWRKTCP
EEECCCCHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHCCCCCCCEEEEEHHHHCCCC
DIALRSTFIVGFPGETEAEFEELLAFLEEAQLDRVGAFTYSPVKGAAANVLPDHVPPEIQ
CCEECCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCEEECCCCCCHHCCCCCCCCHHHH
QERLERLMQQQEIISKQRLAVKKGQQLRVLVDTVDEEGAIARSYADAPEIDGVVYINDAS
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHCCCCCCCCCEEEECCCC
TLKPGDWADVRVTDTDIHDLWTEKI
CCCCCCCCEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA