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The map label for this gene is ada [H]

Identifier: 114331339

GI number: 114331339

Start: 1413562

End: 1414407

Strand: Direct

Name: ada [H]

Synonym: Neut_1346

Alternate gene names: 114331339

Gene position: 1413562-1414407 (Clockwise)

Preceding gene: 114331337

Following gene: 114331340

Centisome position: 53.12

GC content: 45.74

Gene sequence:

>846_bases
ATGATGATCGATCAAGCGCAAACTCATTACGACCGTATTGTCAAAGCAATCGAATATATTCAAGAAAATTTTAAACAGCA
GCCTGCTTTAGAGGATATTGCGGCGGCAGTACATTTAAGTCCGTTTTATTTTCAACGACTGTTTTCTGAATGGGCGGGAG
TGAGTCCCAAGAAATTTGTCCAGTATCTTAGTTTGGAATACGCTAAAAGTTTGTTAAAAAACAGGAATTCCAGCTTGATG
GAAGCAGCGTATGAGACAGGGCTATCGGGAGCCAGCCGATTGCACGATCTCTTTGTGAATATAGAATGCATGACGCCGGG
TGAATTTAAAAATGGTGGCAAAGCCTTGCGTATCGATTACTGTTTTGCTGACAGTCCGTTTGGGAAAGTGATGATCGCAT
CTACCTCCAAGGGAGTTTGCCGGATCGCTTTTGAAGAGGATGAAGTCTTGGCTTTCAGCAATCTTAACAAGAGCTTTCCC
AATGCTTCCTATACACAAAGGGTTAATCAATCTCACCAGGCTGCCCTGTTTATTTTTCAAAAAGATTGGTCACGGCTGGA
TGAAATCAAGCTACATTTAAACGGCACGCCGTTCCAACTCAAAGTTTGGGAATGCTTGCTCAAAATTCCGATGGGCGATC
TAACCACCTACGGCCATATTGCAAAAACAATCGGCAACCCTAAAGCCTCCAGAGCTGTCGGTACGGCAATCAGCCGTAAT
CCCGTCGCATTTCTCATCCCATGCCACAGAGTCATCCAATCCAGTGGGCAGATCGGCGGTTATATGTGGGGGGCCACCCG
AAAATCCGCGATGATTAGTTGGGAAGCCGCGAGAACGGGAGCATGA

Upstream 100 bases:

>100_bases
GCTACAAATTTGATAGTCAGCAAGTTTCGGGTCGTGTTTATGAAAATTCGCTTTTATTCTATGGGGATAGGAAGGATAGA
ATATAACGCGAAGGATAAAA

Downstream 100 bases:

>100_bases
ACATGCAAGACACTCTGAAAAAGATAGCTGCGGCGAATTGGCAACATGTGGCTGATGACATGCACGAAAAAGGCTATGCG
ATCGTGCCCGGTATTTTGTC

Product: transcriptional regulator, AraC family protein

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 281; Mature: 281

Protein sequence:

>281_residues
MMIDQAQTHYDRIVKAIEYIQENFKQQPALEDIAAAVHLSPFYFQRLFSEWAGVSPKKFVQYLSLEYAKSLLKNRNSSLM
EAAYETGLSGASRLHDLFVNIECMTPGEFKNGGKALRIDYCFADSPFGKVMIASTSKGVCRIAFEEDEVLAFSNLNKSFP
NASYTQRVNQSHQAALFIFQKDWSRLDEIKLHLNGTPFQLKVWECLLKIPMGDLTTYGHIAKTIGNPKASRAVGTAISRN
PVAFLIPCHRVIQSSGQIGGYMWGATRKSAMISWEAARTGA

Sequences:

>Translated_281_residues
MMIDQAQTHYDRIVKAIEYIQENFKQQPALEDIAAAVHLSPFYFQRLFSEWAGVSPKKFVQYLSLEYAKSLLKNRNSSLM
EAAYETGLSGASRLHDLFVNIECMTPGEFKNGGKALRIDYCFADSPFGKVMIASTSKGVCRIAFEEDEVLAFSNLNKSFP
NASYTQRVNQSHQAALFIFQKDWSRLDEIKLHLNGTPFQLKVWECLLKIPMGDLTTYGHIAKTIGNPKASRAVGTAISRN
PVAFLIPCHRVIQSSGQIGGYMWGATRKSAMISWEAARTGA
>Mature_281_residues
MMIDQAQTHYDRIVKAIEYIQENFKQQPALEDIAAAVHLSPFYFQRLFSEWAGVSPKKFVQYLSLEYAKSLLKNRNSSLM
EAAYETGLSGASRLHDLFVNIECMTPGEFKNGGKALRIDYCFADSPFGKVMIASTSKGVCRIAFEEDEVLAFSNLNKSFP
NASYTQRVNQSHQAALFIFQKDWSRLDEIKLHLNGTPFQLKVWECLLKIPMGDLTTYGHIAKTIGNPKASRAVGTAISRN
PVAFLIPCHRVIQSSGQIGGYMWGATRKSAMISWEAARTGA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=79, Percent_Identity=44.3037974683544, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1788542, Length=276, Percent_Identity=31.8840579710145, Blast_Score=151, Evalue=6e-38,
Organism=Escherichia coli, GI1787596, Length=98, Percent_Identity=40.8163265306122, Blast_Score=87, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=87, Percent_Identity=43.6781609195402, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI115533070, Length=87, Percent_Identity=43.6781609195402, Blast_Score=90, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=45.7831325301205, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI17137554, Length=181, Percent_Identity=30.3867403314917, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 31463; Mature: 31463

Theoretical pI: Translated: 9.10; Mature: 9.10

Prosite motif: PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMIDQAQTHYDRIVKAIEYIQENFKQQPALEDIAAAVHLSPFYFQRLFSEWAGVSPKKFV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
QYLSLEYAKSLLKNRNSSLMEAAYETGLSGASRLHDLFVNIECMTPGEFKNGGKALRIDY
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHEEEEEEEECCCCCCCCCCEEEEEE
CFADSPFGKVMIASTSKGVCRIAFEEDEVLAFSNLNKSFPNASYTQRVNQSHQAALFIFQ
EECCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHCCCCCEEEEEEH
KDWSRLDEIKLHLNGTPFQLKVWECLLKIPMGDLTTYGHIAKTIGNPKASRAVGTAISRN
HHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCC
PVAFLIPCHRVIQSSGQIGGYMWGATRKSAMISWEAARTGA
CEEEEEEHHHHHHCCCCCCEEEECCCCCCCEEEECHHCCCC
>Mature Secondary Structure
MMIDQAQTHYDRIVKAIEYIQENFKQQPALEDIAAAVHLSPFYFQRLFSEWAGVSPKKFV
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHH
QYLSLEYAKSLLKNRNSSLMEAAYETGLSGASRLHDLFVNIECMTPGEFKNGGKALRIDY
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHEEEEEEEECCCCCCCCCCEEEEEE
CFADSPFGKVMIASTSKGVCRIAFEEDEVLAFSNLNKSFPNASYTQRVNQSHQAALFIFQ
EECCCCCCEEEEEECCCCEEEEEECCCCEEEECCCCCCCCCCHHHHHHCCCCCEEEEEEH
KDWSRLDEIKLHLNGTPFQLKVWECLLKIPMGDLTTYGHIAKTIGNPKASRAVGTAISRN
HHHHHHHHEEEEECCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCC
PVAFLIPCHRVIQSSGQIGGYMWGATRKSAMISWEAARTGA
CEEEEEEHHHHHHCCCCCCEEEECCCCCCCEEEECHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]