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The map label for this gene is potB [H]

Identifier: 114331322

GI number: 114331322

Start: 1392053

End: 1392955

Strand: Direct

Name: potB [H]

Synonym: Neut_1329

Alternate gene names: 114331322

Gene position: 1392053-1392955 (Clockwise)

Preceding gene: 114331321

Following gene: 114331323

Centisome position: 52.31

GC content: 52.38

Gene sequence:

>903_bases
ATGAACAAATTCCGCCTGACACGCTGGTTGATCAGCCTGCCACCTACACTGTTTCTGCTGATATTCTTTGCGGTGCCCAG
CCTGATCATGATTGTGACTTCGTTCCGCTTCCCTGGAGAATATGGCGGGCTGGCACCGTTGACTGCGCCGGAGACAACTG
CCGCAGAAGGGGATTCTGGTTTGTATGGTATGACGCTCGAAGCCTACCACTTCTTTTTTAGCGATATCCTGTACGCAGAA
ATTTTCCTGAAATCCTTTGTGGTTGCGACAACTTCCACCCTGATCTGTCTGATTATGGCCTATCCGGTTGCCATGCTGAT
TGCCCAAAGCGCTAAAAAATACCGCAACCTGATGATATTGCTGGTCGTCCTGCCATTTGCCAGCAATTTTTTGATCCGCA
TCTATGCCTGGATGATTATTCTCGGGCCGGAATCAGCATTCAGCCATTTCATCAATAGTGTACTCCAGGCCTTTGGCTTT
GAGCCGGTCATGATGTTGTTTTCTCCGTTCGCAGTATTGGTCGGAACCGTTTACGTGCACCTACCCTTTATGATTTTGCC
GCTCTACACCAATCTGGAAAAACATGATCCGGTGCTGCTCGATGCTGCACAGGATCTAGGCGCAAACCGCTGGCAGCGCT
TCTGGCTGGTCACCTGGCCACAGTCGCTGCCGGGTGTCTTCTCCGGCTCCGCACTGGTATTCATCCCGGTGCTGGGCATG
TTCGCTATACCGGATATTCTGGGCGGCACCGGTGACATTCTGATCGGCAACCTGATCAAGGATCAGTTCCTGGGAACACG
CGACTGGCCGTTCGGCTCCACATTGTCCATCATGCTCATGCTGGCCGTGCTGTGTGTGACTGGATTATTCACTCTACTGG
CTCGCCCTCGTGTACCCAGGTAA

Upstream 100 bases:

>100_bases
TGGCAAATTCTTCACCCGGACGCGCACGCTTCTTTGAAGTGGATGATCCAGTTATTGTCAGCTGGCCACAGGAAGCTGCA
CAGTTTCTGGTGGATCGGCC

Downstream 100 bases:

>100_bases
ATAAATCTCCTGTTCTGATATGAAACACACCAATCTCACCCTGTGGCTTGCTGCCAGTCTGGTTTATATTTTTCTTTATA
TTCCACTGGTTATTGTCGTC

Product: binding-protein-dependent transport systems inner membrane component

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MNKFRLTRWLISLPPTLFLLIFFAVPSLIMIVTSFRFPGEYGGLAPLTAPETTAAEGDSGLYGMTLEAYHFFFSDILYAE
IFLKSFVVATTSTLICLIMAYPVAMLIAQSAKKYRNLMILLVVLPFASNFLIRIYAWMIILGPESAFSHFINSVLQAFGF
EPVMMLFSPFAVLVGTVYVHLPFMILPLYTNLEKHDPVLLDAAQDLGANRWQRFWLVTWPQSLPGVFSGSALVFIPVLGM
FAIPDILGGTGDILIGNLIKDQFLGTRDWPFGSTLSIMLMLAVLCVTGLFTLLARPRVPR

Sequences:

>Translated_300_residues
MNKFRLTRWLISLPPTLFLLIFFAVPSLIMIVTSFRFPGEYGGLAPLTAPETTAAEGDSGLYGMTLEAYHFFFSDILYAE
IFLKSFVVATTSTLICLIMAYPVAMLIAQSAKKYRNLMILLVVLPFASNFLIRIYAWMIILGPESAFSHFINSVLQAFGF
EPVMMLFSPFAVLVGTVYVHLPFMILPLYTNLEKHDPVLLDAAQDLGANRWQRFWLVTWPQSLPGVFSGSALVFIPVLGM
FAIPDILGGTGDILIGNLIKDQFLGTRDWPFGSTLSIMLMLAVLCVTGLFTLLARPRVPR
>Mature_300_residues
MNKFRLTRWLISLPPTLFLLIFFAVPSLIMIVTSFRFPGEYGGLAPLTAPETTAAEGDSGLYGMTLEAYHFFFSDILYAE
IFLKSFVVATTSTLICLIMAYPVAMLIAQSAKKYRNLMILLVVLPFASNFLIRIYAWMIILGPESAFSHFINSVLQAFGF
EPVMMLFSPFAVLVGTVYVHLPFMILPLYTNLEKHDPVLLDAAQDLGANRWQRFWLVTWPQSLPGVFSGSALVFIPVLGM
FAIPDILGGTGDILIGNLIKDQFLGTRDWPFGSTLSIMLMLAVLCVTGLFTLLARPRVPR

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=266, Percent_Identity=38.7218045112782, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1787080, Length=234, Percent_Identity=39.7435897435897, Blast_Score=166, Evalue=2e-42,
Organism=Escherichia coli, GI1787713, Length=213, Percent_Identity=29.5774647887324, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33438; Mature: 33438

Theoretical pI: Translated: 7.56; Mature: 7.56

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKFRLTRWLISLPPTLFLLIFFAVPSLIMIVTSFRFPGEYGGLAPLTAPETTAAEGDSG
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LYGMTLEAYHFFFSDILYAEIFLKSFVVATTSTLICLIMAYPVAMLIAQSAKKYRNLMIL
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVLPFASNFLIRIYAWMIILGPESAFSHFINSVLQAFGFEPVMMLFSPFAVLVGTVYVH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LPFMILPLYTNLEKHDPVLLDAAQDLGANRWQRFWLVTWPQSLPGVFSGSALVFIPVLGM
HHHHHHHHHHCCCCCCCEEEECHHHHCCHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHH
FAIPDILGGTGDILIGNLIKDQFLGTRDWPFGSTLSIMLMLAVLCVTGLFTLLARPRVPR
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNKFRLTRWLISLPPTLFLLIFFAVPSLIMIVTSFRFPGEYGGLAPLTAPETTAAEGDSG
CCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
LYGMTLEAYHFFFSDILYAEIFLKSFVVATTSTLICLIMAYPVAMLIAQSAKKYRNLMIL
CEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LVVLPFASNFLIRIYAWMIILGPESAFSHFINSVLQAFGFEPVMMLFSPFAVLVGTVYVH
HHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
LPFMILPLYTNLEKHDPVLLDAAQDLGANRWQRFWLVTWPQSLPGVFSGSALVFIPVLGM
HHHHHHHHHHCCCCCCCEEEECHHHHCCHHHHEEEEEECCCCCCCCCCCCHHHHHHHHHH
FAIPDILGGTGDILIGNLIKDQFLGTRDWPFGSTLSIMLMLAVLCVTGLFTLLARPRVPR
HHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]