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The map label for this gene is ddh [H]

Identifier: 114331297

GI number: 114331297

Start: 1368545

End: 1369552

Strand: Direct

Name: ddh [H]

Synonym: Neut_1304

Alternate gene names: 114331297

Gene position: 1368545-1369552 (Clockwise)

Preceding gene: 114331289

Following gene: 114331299

Centisome position: 51.43

GC content: 46.83

Gene sequence:

>1008_bases
ATGCCAATGAAAATCACCTTTTTCAGCACTCAACCGTATGACCGTGAATCATTCCTCAAACATCAAGCCGATTCGCGCCT
TGAACTTATTTTTATGGAAGAAAAACTGACTGAGTACACTGTATCCCTTGCCAGAGGAAGTGAGATAGTTTGTGTTTTCG
TCAACGATACACTTAATGAAAATATTATCCGCCAGCTGTCTCAATTGAATATTCAGCTCATCGCCCTACGCTGCGCCGGC
TTTAATAACGTGGATCTCAAAGCAGCTCAGGCGTGTAATATCAAAGTAGTACGGGTACCAGCCTACTCCCCTCACGCCGT
GGCAGAACATACGCTGGCGATGATCATGACACTCAACCGCAAAACGCATAAGGCATATAACCGTGTCCGCGAACAGAATT
TTTCGCTGAACGGCTTGCTTGGTTTCGATCTCTACAAGAAAACAGTGGGCGTCATTGGTACCGGCCATATCGGGGAAGTA
TTCTGCCGCATCCTGCATGGCCTGGGCTGCAACATTTTGGCCTGTGATCCGATAAAAAAACCAACTGTGGAAGCAATGGG
CATTCCGTATGTCCCCATGAGCGAGCTCTTCCCCCAATGCGATATTCTTTCGCTGCACTGCCCACTCAATGAAGAAACAC
GCTACCTCATCAACAACGAAACGATTGCACAAATGAAAGCGGGCATCATGCTGATCAACACCAGCCGTGGCGGATTAATC
GACACCAAGGCCGTAATCACCGGGTTAAAAACAGGAAAAATCAGTTATCTGGGTATTGATGTCTACGAACAGGAAGCCGA
TCTGTTTTTCCAAAACCTCTCTGAACAGATCCTCCATGATGACACTATTGCCCGGCTCATGACCTTTCCCAATGTCCTGA
TTACTGCGCACCAGGGGTTTTTTACCCAGGAAGCACTCAGTCAGATTGCCCTTACAACACTGGATAACGTAAGGCAGTTT
ATAGATGGAGAGGTGCTGATTAATGCAGTCAGTAAGGACAATGGATAA

Upstream 100 bases:

>100_bases
GCATTACCCTGTTTGACATTCCGGGCATTTCCCGATACGTTATTTTCATCGAAAGAATCACCCCTTGCCCTCTATTTATC
TTGCTGTTCCTGCCGGAAAT

Downstream 100 bases:

>100_bases
TCTTCCAATATGGGGTCGTCTCAGAATTCGGAAAATAAAGCACGCTAAGGCGTAGTCACCCCGTGACTCCCCCGCGCCGA
TGCAGCGAGCTTCGTTCCGT

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MPMKITFFSTQPYDRESFLKHQADSRLELIFMEEKLTEYTVSLARGSEIVCVFVNDTLNENIIRQLSQLNIQLIALRCAG
FNNVDLKAAQACNIKVVRVPAYSPHAVAEHTLAMIMTLNRKTHKAYNRVREQNFSLNGLLGFDLYKKTVGVIGTGHIGEV
FCRILHGLGCNILACDPIKKPTVEAMGIPYVPMSELFPQCDILSLHCPLNEETRYLINNETIAQMKAGIMLINTSRGGLI
DTKAVITGLKTGKISYLGIDVYEQEADLFFQNLSEQILHDDTIARLMTFPNVLITAHQGFFTQEALSQIALTTLDNVRQF
IDGEVLINAVSKDNG

Sequences:

>Translated_335_residues
MPMKITFFSTQPYDRESFLKHQADSRLELIFMEEKLTEYTVSLARGSEIVCVFVNDTLNENIIRQLSQLNIQLIALRCAG
FNNVDLKAAQACNIKVVRVPAYSPHAVAEHTLAMIMTLNRKTHKAYNRVREQNFSLNGLLGFDLYKKTVGVIGTGHIGEV
FCRILHGLGCNILACDPIKKPTVEAMGIPYVPMSELFPQCDILSLHCPLNEETRYLINNETIAQMKAGIMLINTSRGGLI
DTKAVITGLKTGKISYLGIDVYEQEADLFFQNLSEQILHDDTIARLMTFPNVLITAHQGFFTQEALSQIALTTLDNVRQF
IDGEVLINAVSKDNG
>Mature_334_residues
PMKITFFSTQPYDRESFLKHQADSRLELIFMEEKLTEYTVSLARGSEIVCVFVNDTLNENIIRQLSQLNIQLIALRCAGF
NNVDLKAAQACNIKVVRVPAYSPHAVAEHTLAMIMTLNRKTHKAYNRVREQNFSLNGLLGFDLYKKTVGVIGTGHIGEVF
CRILHGLGCNILACDPIKKPTVEAMGIPYVPMSELFPQCDILSLHCPLNEETRYLINNETIAQMKAGIMLINTSRGGLID
TKAVITGLKTGKISYLGIDVYEQEADLFFQNLSEQILHDDTIARLMTFPNVLITAHQGFFTQEALSQIALTTLDNVRQFI
DGEVLINAVSKDNG

Specific function: Fermentative Lactate Dehydrogenase. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=268, Percent_Identity=31.7164179104478, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI4557497, Length=268, Percent_Identity=31.7164179104478, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI23308577, Length=254, Percent_Identity=30.7086614173228, Blast_Score=129, Evalue=3e-30,
Organism=Homo sapiens, GI145580578, Length=267, Percent_Identity=30.3370786516854, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI4557499, Length=267, Percent_Identity=30.3370786516854, Blast_Score=122, Evalue=3e-28,
Organism=Homo sapiens, GI145580575, Length=267, Percent_Identity=30.3370786516854, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI6912396, Length=331, Percent_Identity=24.1691842900302, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1787645, Length=326, Percent_Identity=49.079754601227, Blast_Score=327, Evalue=7e-91,
Organism=Escherichia coli, GI87082289, Length=287, Percent_Identity=26.8292682926829, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1789279, Length=279, Percent_Identity=29.7491039426523, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1788660, Length=278, Percent_Identity=25.5395683453237, Blast_Score=70, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17532191, Length=257, Percent_Identity=29.1828793774319, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=253, Percent_Identity=27.2727272727273, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324055, Length=255, Percent_Identity=29.8039215686275, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6322116, Length=267, Percent_Identity=28.8389513108614, Blast_Score=112, Evalue=9e-26,
Organism=Saccharomyces cerevisiae, GI6320925, Length=255, Percent_Identity=29.0196078431373, Blast_Score=111, Evalue=2e-25,
Organism=Drosophila melanogaster, GI19921140, Length=253, Percent_Identity=31.2252964426877, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI24646446, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646448, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646452, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24646450, Length=269, Percent_Identity=32.3420074349442, Blast_Score=129, Evalue=3e-30,
Organism=Drosophila melanogaster, GI62472511, Length=277, Percent_Identity=31.7689530685921, Blast_Score=127, Evalue=8e-30,
Organism=Drosophila melanogaster, GI28574286, Length=334, Percent_Identity=24.5508982035928, Blast_Score=92, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45552429, Length=264, Percent_Identity=24.2424242424242, Blast_Score=85, Evalue=8e-17,
Organism=Drosophila melanogaster, GI24585514, Length=264, Percent_Identity=24.2424242424242, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574282, Length=264, Percent_Identity=24.2424242424242, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28574284, Length=264, Percent_Identity=23.8636363636364, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45551003, Length=264, Percent_Identity=23.8636363636364, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571528, Length=295, Percent_Identity=24.7457627118644, Blast_Score=76, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585516, Length=261, Percent_Identity=23.3716475095785, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.28

Molecular weight: Translated: 37458; Mature: 37327

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00670 D_2_HYDROXYACID_DH_2 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPMKITFFSTQPYDRESFLKHQADSRLELIFMEEKLTEYTVSLARGSEIVCVFVNDTLNE
CCEEEEEEECCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCH
NIIRQLSQLNIQLIALRCAGFNNVDLKAAQACNIKVVRVPAYSPHAVAEHTLAMIMTLNR
HHHHHHHHCCEEEEEEEECCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
KTHKAYNRVREQNFSLNGLLGFDLYKKTVGVIGTGHIGEVFCRILHGLGCNILACDPIKK
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCEECCHHHHHHHHHHHCCCCCEEEECCCCC
PTVEAMGIPYVPMSELFPQCDILSLHCPLNEETRYLINNETIAQMKAGIMLINTSRGGLI
CCHHHCCCCCCCHHHHCCCCCEEEEECCCCCCCEEEECCCHHHHHHCCEEEEECCCCCEE
DTKAVITGLKTGKISYLGIDVYEQEADLFFQNLSEQILHDDTIARLMTFPNVLITAHQGF
EHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
FTQEALSQIALTTLDNVRQFIDGEVLINAVSKDNG
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
>Mature Secondary Structure 
PMKITFFSTQPYDRESFLKHQADSRLELIFMEEKLTEYTVSLARGSEIVCVFVNDTLNE
CEEEEEEECCCCCHHHHHHHCCCCCEEEEEEHHHHHHHHHHHCCCCEEEEEEECCCCCH
NIIRQLSQLNIQLIALRCAGFNNVDLKAAQACNIKVVRVPAYSPHAVAEHTLAMIMTLNR
HHHHHHHHCCEEEEEEEECCCCCCCCEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHCC
KTHKAYNRVREQNFSLNGLLGFDLYKKTVGVIGTGHIGEVFCRILHGLGCNILACDPIKK
HHHHHHHHHHHCCCCCCEEEEHHHHHHHHCCEECCHHHHHHHHHHHCCCCCEEEECCCCC
PTVEAMGIPYVPMSELFPQCDILSLHCPLNEETRYLINNETIAQMKAGIMLINTSRGGLI
CCHHHCCCCCCCHHHHCCCCCEEEEECCCCCCCEEEECCCHHHHHHCCEEEEECCCCCEE
DTKAVITGLKTGKISYLGIDVYEQEADLFFQNLSEQILHDDTIARLMTFPNVLITAHQGF
EHHHHHHCCCCCEEEEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCC
FTQEALSQIALTTLDNVRQFIDGEVLINAVSKDNG
HHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: Pyruvate [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]