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The map label for this gene is ygfZ [C]

Identifier: 114331277

GI number: 114331277

Start: 1344157

End: 1345227

Strand: Direct

Name: ygfZ [C]

Synonym: Neut_1283

Alternate gene names: 114331277

Gene position: 1344157-1345227 (Clockwise)

Preceding gene: 114331274

Following gene: 114331281

Centisome position: 50.51

GC content: 49.77

Gene sequence:

>1071_bases
ATGAACCCTGACTGGTTTACATTTTTGACAAGCCGCAATGCCCATATCGAACAAAATCGGGTGTTGCATTTTGGTCAACC
CGACGTTGAATTGGCACAAGTAGAATCAGCATCAGTGCTGATTGATCTCTCCCACCTCGGTCTGATCCGCTTTTCTGGCG
AAGAGACACAAAAATTTTTGCAGGGGCAGTTAAGCTGTGATGTTCACACCACCGATTCCGGCAAAGCCACTTATGGCGGC
TACTGTACGCCTAAAGGTCGTCTGTTGAGCAGCTTTTTGCTATGGCAGAACATTTCCGACTACAGCTACCTGATGCAGCT
CCCGGCAGAACTGACGGAAACCATTGCCAAACGACTGAAAATGTTTGTACTGCGAGCGAAGGTGATCATTCAGGATCATA
CCGAAGATTGTATTCGTATTGGTGTTGCCGGCAAGAATGCTCACACATTGCTGCAAAATACGTTGGCAGGAACGGTTCTC
CCCACCCAGCCCTTGGCTATAACAGCCATTCCAGACGGGCAGGTAATTTGTCACAGTGAAAACCGGTTTGAAATTCTGAT
TTCCCCGGCCCACGCACTTTCATTATGGGAGCGACTAAGCAGTCAGGCACGTTGCGCGGGTGCCGCCGCCTGGGACTGGC
TGGAAATTCAGGAAGGAGTCCCGGCAATTTTCAAAGCTACCCAGGAGCAGTTCATCCCGCAAATGATCAACCTGGATGCA
ATTGGTGGGGTCAATTTCAAAAAAGGGTGTTATCCCGGCCAGGAAATCGTTGCTCGTACGCAATATCTTGGCAAGGTGAA
GCGCCGCATGTACCGGGCTCATCTGGATTCAGATTCACCCCTGGAGATTACGGCAGGTGACAACCTGTTCAGTGCAGATA
CTGGTGGTCAGGCATGTGGAATGATCGTTAATGCTGCTCCTGCTCCTGCTCCTGCTAAAGGCGTTGATGTATTGGCAGTG
ATTCAGGTCAGCAGCATAGAAGCTAATCCAATTCACTGCAAAACACCGGATGGTCCGCAGTTGACTATCCAGTCGCTTCC
TTATTCAATACCTGATCAGCACTCCCATTAA

Upstream 100 bases:

>100_bases
CTCAGCAACACTGCCAACTGTCTGGAAGGTTGCAGCTGAATTGACAGCGCACTCTCCGATTGATTATTGATATCACCCAA
CGTAATTTTCCTTTCTTTCC

Downstream 100 bases:

>100_bases
GCAAAAGACAACAGCAATTTAATCAGTCTTGCCGTTTATGCGTCAGTAACTGCACGATATCAGTATGTCCGCGTTTCTGG
GCCCAAGCCAATGCCGTACC

Product: glycine cleavage T protein (aminomethyl transferase)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MNPDWFTFLTSRNAHIEQNRVLHFGQPDVELAQVESASVLIDLSHLGLIRFSGEETQKFLQGQLSCDVHTTDSGKATYGG
YCTPKGRLLSSFLLWQNISDYSYLMQLPAELTETIAKRLKMFVLRAKVIIQDHTEDCIRIGVAGKNAHTLLQNTLAGTVL
PTQPLAITAIPDGQVICHSENRFEILISPAHALSLWERLSSQARCAGAAAWDWLEIQEGVPAIFKATQEQFIPQMINLDA
IGGVNFKKGCYPGQEIVARTQYLGKVKRRMYRAHLDSDSPLEITAGDNLFSADTGGQACGMIVNAAPAPAPAKGVDVLAV
IQVSSIEANPIHCKTPDGPQLTIQSLPYSIPDQHSH

Sequences:

>Translated_356_residues
MNPDWFTFLTSRNAHIEQNRVLHFGQPDVELAQVESASVLIDLSHLGLIRFSGEETQKFLQGQLSCDVHTTDSGKATYGG
YCTPKGRLLSSFLLWQNISDYSYLMQLPAELTETIAKRLKMFVLRAKVIIQDHTEDCIRIGVAGKNAHTLLQNTLAGTVL
PTQPLAITAIPDGQVICHSENRFEILISPAHALSLWERLSSQARCAGAAAWDWLEIQEGVPAIFKATQEQFIPQMINLDA
IGGVNFKKGCYPGQEIVARTQYLGKVKRRMYRAHLDSDSPLEITAGDNLFSADTGGQACGMIVNAAPAPAPAKGVDVLAV
IQVSSIEANPIHCKTPDGPQLTIQSLPYSIPDQHSH
>Mature_356_residues
MNPDWFTFLTSRNAHIEQNRVLHFGQPDVELAQVESASVLIDLSHLGLIRFSGEETQKFLQGQLSCDVHTTDSGKATYGG
YCTPKGRLLSSFLLWQNISDYSYLMQLPAELTETIAKRLKMFVLRAKVIIQDHTEDCIRIGVAGKNAHTLLQNTLAGTVL
PTQPLAITAIPDGQVICHSENRFEILISPAHALSLWERLSSQARCAGAAAWDWLEIQEGVPAIFKATQEQFIPQMINLDA
IGGVNFKKGCYPGQEIVARTQYLGKVKRRMYRAHLDSDSPLEITAGDNLFSADTGGQACGMIVNAAPAPAPAKGVDVLAV
IQVSSIEANPIHCKTPDGPQLTIQSLPYSIPDQHSH

Specific function: Folate-binding protein involved in regulating the level of ATP-dnaA and in the modification of some tRNAs. It is probably a key factor in regulatory networks that act via tRNA modification, such as initiation of chromosomal replication [H]

COG id: COG0354

COG function: function code R; Predicted aminomethyltransferase related to GcvT

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA-modifying ygfZ family [H]

Homologues:

Organism=Escherichia coli, GI1789265, Length=324, Percent_Identity=28.3950617283951, Blast_Score=121, Evalue=8e-29,

Paralogues:

None

Copy number: 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017703
- InterPro:   IPR006222 [H]

Pfam domain/function: PF01571 GCV_T [H]

EC number: NA

Molecular weight: Translated: 38982; Mature: 38982

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPDWFTFLTSRNAHIEQNRVLHFGQPDVELAQVESASVLIDLSHLGLIRFSGEETQKFL
CCCCEEEEEECCCCCCCCCCEEECCCCCCEEEEECCCEEEEEECCCEEEEECCHHHHHHH
QGQLSCDVHTTDSGKATYGGYCTPKGRLLSSFLLWQNISDYSYLMQLPAELTETIAKRLK
CCCCEEEEEECCCCCCCCCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MFVLRAKVIIQDHTEDCIRIGVAGKNAHTLLQNTLAGTVLPTQPLAITAIPDGQVICHSE
HHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECC
NRFEILISPAHALSLWERLSSQARCAGAAAWDWLEIQEGVPAIFKATQEQFIPQMINLDA
CCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCC
IGGVNFKKGCYPGQEIVARTQYLGKVKRRMYRAHLDSDSPLEITAGDNLFSADTGGQACG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCEEE
MIVNAAPAPAPAKGVDVLAVIQVSSIEANPIHCKTPDGPQLTIQSLPYSIPDQHSH
EEEECCCCCCCCCCCCEEEEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MNPDWFTFLTSRNAHIEQNRVLHFGQPDVELAQVESASVLIDLSHLGLIRFSGEETQKFL
CCCCEEEEEECCCCCCCCCCEEECCCCCCEEEEECCCEEEEEECCCEEEEECCHHHHHHH
QGQLSCDVHTTDSGKATYGGYCTPKGRLLSSFLLWQNISDYSYLMQLPAELTETIAKRLK
CCCCEEEEEECCCCCCCCCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
MFVLRAKVIIQDHTEDCIRIGVAGKNAHTLLQNTLAGTVLPTQPLAITAIPDGQVICHSE
HHHHHHHHEEECCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCEEEECC
NRFEILISPAHALSLWERLSSQARCAGAAAWDWLEIQEGVPAIFKATQEQFIPQMINLDA
CCEEEEEECHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCC
IGGVNFKKGCYPGQEIVARTQYLGKVKRRMYRAHLDSDSPLEITAGDNLFSADTGGQACG
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEECCCCCCEEE
MIVNAAPAPAPAKGVDVLAVIQVSSIEANPIHCKTPDGPQLTIQSLPYSIPDQHSH
EEEECCCCCCCCCCCCEEEEEEEECCCCCCEEECCCCCCEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA