Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 114331228

GI number: 114331228

Start: 1288604

End: 1290010

Strand: Direct

Name: glnA [H]

Synonym: Neut_1231

Alternate gene names: 114331228

Gene position: 1288604-1290010 (Clockwise)

Preceding gene: 114331227

Following gene: 114331229

Centisome position: 48.42

GC content: 47.69

Gene sequence:

>1407_bases
GTGGCAGCTGATGTTTTAAAGATGATTCAGGACCATGAAGTCAAGTTCATTGATCTGCGCTTCACCGATACCAACGGCAG
GGAACATCATGTCACAGTTCCCGCACATGTCTTGGATGCCGATAAATTCGAGGATGGTCATCCGTTCGATGGATCCTCAA
TTGCCGGCTGGAAAAACATTCACGCATCCGATATGTTGCTTATACCTGATCCAGAAACTGCTTCGCTGGATCCTTTTATG
GAAGAAACCACATTGCTGCTAACTTGCGATGTGATTGATCCTGCTGATGGGCAGGGATACAACCGCGACCCGCGATCACT
GGCCAGACGGGGAGAAACTTATCTGAAATCCACCGGCATTGGTGACGTTGCCTACTTTGGCCCGGAACTGGAATTTTTTA
TTTTTGACTCGGTACGCTGGCACACCGACATGTCTGGGTGTTTTGTCAAGATTGAATCAGAAGAAGCTTCCTGGAGCTCC
AAAAAACGCTATGAAGGCAGTAATAATGGTTATCGTCCCGCAATCAAAGGCGGATATATGCCGGTGCCACCAGTAGATTC
TCTGCACGACATTCGCTCAGCTATGTGTATGGTGCTTGATGAACTGGGAATCCCTGTAGAAGTTCATCACCACGAAGTTG
CCAATGCCGGACAATGTGAAATTGGTACCCGCTTCAACAGCCTGGTGAAACGCGCCGATTGGAATCAACTGATGAAGTAC
GTCATTCATAATATTGCTGTTTCCTACGGCAAAACAGCAACATTCATGCCCAAGCCAATTGTTGGTGACAATGGTTCGGG
TATGCATGTCCATCAGTCAATCTGGAAGGACGGTAAAAACCTGTTTGCAGGGAACGGTTATGCCGGCATGTCTGAAATCG
CGCTGTATTATATCGGGGGCATTATCCGACATGCCAAAGCACTGAATGCTTTTGCCAATCCAGGCACCAATTCGTATAAA
AGGCTGGTTGCCGGCTTTGAAGCCCCTGTCAATATTGCTTATTCTTCCAGCAACCGGTCAGCCGCTGTGCGTATCCCCTA
TGCTAGCAGCCCAAAACAGCGCCGTATTGAAATACGCTTCCCCGATCCCAGTGCCAACCCTTATCTGACCTTTACCGCCC
TACTGATGGCAGGGCTAGATGGTATACAAAACAAAATCCATCCAGGCGATCCGATGGATAAAAATCTGTATGATCTGCCC
CCGGAAGAAGCCTCGATGGTACCCAATACCTGCACATCACTCGAAGAAGCTCTGGAAAGCCTGGATCAGGATCGTGAATT
TTTGACACGCGGTAACGTATTTTCCAACGATATGATTGACGCCTTTATCAAGCTTAAATCAGAAGAGGCAATATTGTTAC
GTACCACTACACATCCTGTTGAGTTTGCCTTGTACTACAGCCTGTAA

Upstream 100 bases:

>100_bases
ACAATACCGGCCAACGTACCTTCCTTGCATTTTCTCTGCCAAGCTCGTGTACCTTACAAACCGTTTCACAAGTAAATGTT
ATTTTTCTGGAGAAAAAACT

Downstream 100 bases:

>100_bases
AAAGACGGTATTAATCAACGCTTATTCACAGATCACCAACAACGCCACCCGACTTAAAATCTGATATTTTCCGGACAGAA
CTTCGTATTTCGCTAATTCG

Product: glutamine synthetase, type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MAADVLKMIQDHEVKFIDLRFTDTNGREHHVTVPAHVLDADKFEDGHPFDGSSIAGWKNIHASDMLLIPDPETASLDPFM
EETTLLLTCDVIDPADGQGYNRDPRSLARRGETYLKSTGIGDVAYFGPELEFFIFDSVRWHTDMSGCFVKIESEEASWSS
KKRYEGSNNGYRPAIKGGYMPVPPVDSLHDIRSAMCMVLDELGIPVEVHHHEVANAGQCEIGTRFNSLVKRADWNQLMKY
VIHNIAVSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGNGYAGMSEIALYYIGGIIRHAKALNAFANPGTNSYK
RLVAGFEAPVNIAYSSSNRSAAVRIPYASSPKQRRIEIRFPDPSANPYLTFTALLMAGLDGIQNKIHPGDPMDKNLYDLP
PEEASMVPNTCTSLEEALESLDQDREFLTRGNVFSNDMIDAFIKLKSEEAILLRTTTHPVEFALYYSL

Sequences:

>Translated_468_residues
MAADVLKMIQDHEVKFIDLRFTDTNGREHHVTVPAHVLDADKFEDGHPFDGSSIAGWKNIHASDMLLIPDPETASLDPFM
EETTLLLTCDVIDPADGQGYNRDPRSLARRGETYLKSTGIGDVAYFGPELEFFIFDSVRWHTDMSGCFVKIESEEASWSS
KKRYEGSNNGYRPAIKGGYMPVPPVDSLHDIRSAMCMVLDELGIPVEVHHHEVANAGQCEIGTRFNSLVKRADWNQLMKY
VIHNIAVSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGNGYAGMSEIALYYIGGIIRHAKALNAFANPGTNSYK
RLVAGFEAPVNIAYSSSNRSAAVRIPYASSPKQRRIEIRFPDPSANPYLTFTALLMAGLDGIQNKIHPGDPMDKNLYDLP
PEEASMVPNTCTSLEEALESLDQDREFLTRGNVFSNDMIDAFIKLKSEEAILLRTTTHPVEFALYYSL
>Mature_467_residues
AADVLKMIQDHEVKFIDLRFTDTNGREHHVTVPAHVLDADKFEDGHPFDGSSIAGWKNIHASDMLLIPDPETASLDPFME
ETTLLLTCDVIDPADGQGYNRDPRSLARRGETYLKSTGIGDVAYFGPELEFFIFDSVRWHTDMSGCFVKIESEEASWSSK
KRYEGSNNGYRPAIKGGYMPVPPVDSLHDIRSAMCMVLDELGIPVEVHHHEVANAGQCEIGTRFNSLVKRADWNQLMKYV
IHNIAVSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGNGYAGMSEIALYYIGGIIRHAKALNAFANPGTNSYKR
LVAGFEAPVNIAYSSSNRSAAVRIPYASSPKQRRIEIRFPDPSANPYLTFTALLMAGLDGIQNKIHPGDPMDKNLYDLPP
EEASMVPNTCTSLEEALESLDQDREFLTRGNVFSNDMIDAFIKLKSEEAILLRTTTHPVEFALYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=467, Percent_Identity=63.5974304068523, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=394, Percent_Identity=25.1269035532995, Blast_Score=82, Evalue=6e-17,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52033; Mature: 51902

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAADVLKMIQDHEVKFIDLRFTDTNGREHHVTVPAHVLDADKFEDGHPFDGSSIAGWKNI
CHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCHHEECCCCCCCCCCCCCCCCCCCCCC
HASDMLLIPDPETASLDPFMEETTLLLTCDVIDPADGQGYNRDPRSLARRGETYLKSTGI
CCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHCCC
GDVAYFGPELEFFIFDSVRWHTDMSGCFVKIESEEASWSSKKRYEGSNNGYRPAIKGGYM
CCEEEECCCEEEEEEECEEEECCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCC
PVPPVDSLHDIRSAMCMVLDELGIPVEVHHHEVANAGQCEIGTRFNSLVKRADWNQLMKY
CCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECHHHHHHHHHCCHHHHHHH
VIHNIAVSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGNGYAGMSEIALYYIGG
HHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHH
IIRHAKALNAFANPGTNSYKRLVAGFEAPVNIAYSSSNRSAAVRIPYASSPKQRRIEIRF
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEEC
PDPSANPYLTFTALLMAGLDGIQNKIHPGDPMDKNLYDLPPEEASMVPNTCTSLEEALES
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
LDQDREFLTRGNVFSNDMIDAFIKLKSEEAILLRTTTHPVEFALYYSL
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEC
>Mature Secondary Structure 
AADVLKMIQDHEVKFIDLRFTDTNGREHHVTVPAHVLDADKFEDGHPFDGSSIAGWKNI
HHHHHHHHHCCCEEEEEEEEECCCCCEEEEECCHHEECCCCCCCCCCCCCCCCCCCCCC
HASDMLLIPDPETASLDPFMEETTLLLTCDVIDPADGQGYNRDPRSLARRGETYLKSTGI
CCCCEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHCCHHHHHHCCC
GDVAYFGPELEFFIFDSVRWHTDMSGCFVKIESEEASWSSKKRYEGSNNGYRPAIKGGYM
CCEEEECCCEEEEEEECEEEECCCCCEEEEEECCCCCCCHHHCCCCCCCCCCCCCCCCCC
PVPPVDSLHDIRSAMCMVLDELGIPVEVHHHEVANAGQCEIGTRFNSLVKRADWNQLMKY
CCCCCHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEECHHHHHHHHHCCHHHHHHH
VIHNIAVSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGNGYAGMSEIALYYIGG
HHHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCHHHHHHHHHHH
IIRHAKALNAFANPGTNSYKRLVAGFEAPVNIAYSSSNRSAAVRIPYASSPKQRRIEIRF
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCCCEEEEECCCCCCCCCEEEEEC
PDPSANPYLTFTALLMAGLDGIQNKIHPGDPMDKNLYDLPPEEASMVPNTCTSLEEALES
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHHHHHH
LDQDREFLTRGNVFSNDMIDAFIKLKSEEAILLRTTTHPVEFALYYSL
HHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEEECCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1969923 [H]