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The map label for this gene is yfgF [H]

Identifier: 114331197

GI number: 114331197

Start: 1254271

End: 1255794

Strand: Direct

Name: yfgF [H]

Synonym: Neut_1199

Alternate gene names: 114331197

Gene position: 1254271-1255794 (Clockwise)

Preceding gene: 114331196

Following gene: 114331201

Centisome position: 47.13

GC content: 40.42

Gene sequence:

>1524_bases
ATGTATATCCACTCAAAAATCAATACGATTAAACCTTTTTTGCCGACGGCTGTAACAATGGTTGTTGCCGGTTTATTGAT
CGCGCTGGATTATTCCATGCTCTCTATCGAGCCACATATCATTGTAGGGCTATGGGTAATTCTGGTAACAGCAATCCTGA
TTACAATTATTCAGATTGTGCATGCTGAAAAAATATTTTCAATACAATTTGCACAGTTGACTGCTCAAAAAGAACGTCTT
GCCAATGAAATAAAATATCGAATCTGGGCCGAAAAAACTTCTTCGGAGAATAAAGCCAAGCTACAAATTGTAGATGAGAA
TTTTCCGGTCATGCTAGCTTACTTTAATCCGGAGCAGCAATGCCGCTATCACAATCGGGCTTTTCGTCAATGGTTTGGAT
TAAGGTCTGAACAAATAGAAAATCGATTTTTGCATGAGTTTCTCAGTGGATCATTTTATACCCATATCAAGCATGAAGTC
GGACGCGTATTATCTGGCGAAACGGTGCAAGGTCAACAAATTCAGAAACTTATAAATCAATCTGCCTGTTTGGTTACCGC
CCATTTAATACCGCATTTCGATTCTGGCGGAAAGGTAATCGGGTTTTATACTCTTTATACGCCTCGGTTTATGAAGAAAG
ATGGGCTGTCTCTGGAGAGTTTTAAAGAAAAATCTGTATCTGAGAATGAGCAATCGTGGAAAAGAAAGCTTACCCAACAC
TCAGGTGGAGATGCAACACATCAACCGGCACGAGTATCTATAGACTCATCCAGCCGTATTATCCTGGCTATTGAGCAAGA
GGAGTTCCGTCTTTTTTGTCAGAGAATACTGCCGGTTAAACCTGGTCAGAATGCTACAACCTACTACGAAATCCTTATTC
GTATGGCAGAGGAAGAGTCTAATCTTATTCCCCCAGGCGCGTTTCTCCCGCTTGTCGAAAAACACAACCTGATGCCGCGT
CTGGACGGATGGGTCGTGAAGAAAATTATCCAATACCTTTCAAAAAAGAATGTTAGTACTGAAATTTCATTTTGTATAAA
TTTATCTAACAGCACACTTAAGGATCCGGCGTTTGCTGATCACGTTCAAGATCTCCTGAAGAACGCCAAAGTACCGCCTG
ATAAATTATGTTTTGAAATAGAAGCATTTGATGTTATAGCTAATTTGCAAAATACAGTACTATTCGTAAAGAAAATGCAG
CAATTAGGGTGCCTGGTTTCATTATGCAGCTTCAATCATGACCGTACATCATTTGATTTGCTGAAGCACATCAGGGCAGA
TTTTTTGAAGATAGATGGCAGCATAGTTTGCAACATTTTACGGGATGCGGAAGATCTGAAAAAAATTGAAAATATCAACA
AATTTGCGCGTGCTCTGAAGATCGGGACAATAGGAGAGCTGGTAGAAACACAAGCTATTCTGGAAAAACTCGCAGAAATT
GGGATCGATTATGCTCAAGGATTTGCTGTCGGGCAGCCGCATCCGATTGAAACCATAAGTCGCTCCACTATCAACTTACC
ATGA

Upstream 100 bases:

>100_bases
TTTGGAAATTTTGGGCTTCAGTACGTCCGTTTGGATCTTATAGTTAGTTGTGGTCAGTTTGGGGAAATTAAATTTTTTTA
ACGAGACTTTCCTCATAGCT

Downstream 100 bases:

>100_bases
GTTGCAAGTATATTAATTACTGTGCCAACACTTGATATGCTTCCTGATATTTTTCTACTGTTTGCATGAGAATTTCAGCC
GGGATAGCAGGAGCAGGTGG

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MYIHSKINTIKPFLPTAVTMVVAGLLIALDYSMLSIEPHIIVGLWVILVTAILITIIQIVHAEKIFSIQFAQLTAQKERL
ANEIKYRIWAEKTSSENKAKLQIVDENFPVMLAYFNPEQQCRYHNRAFRQWFGLRSEQIENRFLHEFLSGSFYTHIKHEV
GRVLSGETVQGQQIQKLINQSACLVTAHLIPHFDSGGKVIGFYTLYTPRFMKKDGLSLESFKEKSVSENEQSWKRKLTQH
SGGDATHQPARVSIDSSSRIILAIEQEEFRLFCQRILPVKPGQNATTYYEILIRMAEEESNLIPPGAFLPLVEKHNLMPR
LDGWVVKKIIQYLSKKNVSTEISFCINLSNSTLKDPAFADHVQDLLKNAKVPPDKLCFEIEAFDVIANLQNTVLFVKKMQ
QLGCLVSLCSFNHDRTSFDLLKHIRADFLKIDGSIVCNILRDAEDLKKIENINKFARALKIGTIGELVETQAILEKLAEI
GIDYAQGFAVGQPHPIETISRSTINLP

Sequences:

>Translated_507_residues
MYIHSKINTIKPFLPTAVTMVVAGLLIALDYSMLSIEPHIIVGLWVILVTAILITIIQIVHAEKIFSIQFAQLTAQKERL
ANEIKYRIWAEKTSSENKAKLQIVDENFPVMLAYFNPEQQCRYHNRAFRQWFGLRSEQIENRFLHEFLSGSFYTHIKHEV
GRVLSGETVQGQQIQKLINQSACLVTAHLIPHFDSGGKVIGFYTLYTPRFMKKDGLSLESFKEKSVSENEQSWKRKLTQH
SGGDATHQPARVSIDSSSRIILAIEQEEFRLFCQRILPVKPGQNATTYYEILIRMAEEESNLIPPGAFLPLVEKHNLMPR
LDGWVVKKIIQYLSKKNVSTEISFCINLSNSTLKDPAFADHVQDLLKNAKVPPDKLCFEIEAFDVIANLQNTVLFVKKMQ
QLGCLVSLCSFNHDRTSFDLLKHIRADFLKIDGSIVCNILRDAEDLKKIENINKFARALKIGTIGELVETQAILEKLAEI
GIDYAQGFAVGQPHPIETISRSTINLP
>Mature_507_residues
MYIHSKINTIKPFLPTAVTMVVAGLLIALDYSMLSIEPHIIVGLWVILVTAILITIIQIVHAEKIFSIQFAQLTAQKERL
ANEIKYRIWAEKTSSENKAKLQIVDENFPVMLAYFNPEQQCRYHNRAFRQWFGLRSEQIENRFLHEFLSGSFYTHIKHEV
GRVLSGETVQGQQIQKLINQSACLVTAHLIPHFDSGGKVIGFYTLYTPRFMKKDGLSLESFKEKSVSENEQSWKRKLTQH
SGGDATHQPARVSIDSSSRIILAIEQEEFRLFCQRILPVKPGQNATTYYEILIRMAEEESNLIPPGAFLPLVEKHNLMPR
LDGWVVKKIIQYLSKKNVSTEISFCINLSNSTLKDPAFADHVQDLLKNAKVPPDKLCFEIEAFDVIANLQNTVLFVKKMQ
QLGCLVSLCSFNHDRTSFDLLKHIRADFLKIDGSIVCNILRDAEDLKKIENINKFARALKIGTIGELVETQAILEKLAEI
GIDYAQGFAVGQPHPIETISRSTINLP

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: COG2200

COG function: function code T; FOG: EAL domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=259, Percent_Identity=30.1158301158301, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI87082096, Length=235, Percent_Identity=26.3829787234043, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI87081980, Length=241, Percent_Identity=26.9709543568465, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI226510982, Length=337, Percent_Identity=23.7388724035608, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1790496, Length=248, Percent_Identity=24.5967741935484, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1788502, Length=252, Percent_Identity=23.015873015873, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1786507, Length=238, Percent_Identity=24.3697478991597, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI87081845, Length=234, Percent_Identity=24.7863247863248, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI87081743, Length=247, Percent_Identity=23.4817813765182, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=213, Percent_Identity=24.8826291079812, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788381, Length=183, Percent_Identity=26.2295081967213, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 57606; Mature: 57606

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYIHSKINTIKPFLPTAVTMVVAGLLIALDYSMLSIEPHIIVGLWVILVTAILITIIQIV
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
HAEKIFSIQFAQLTAQKERLANEIKYRIWAEKTSSENKAKLQIVDENFPVMLAYFNPEQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCEEEEEECCHHH
CRYHNRAFRQWFGLRSEQIENRFLHEFLSGSFYTHIKHEVGRVLSGETVQGQQIQKLINQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
SACLVTAHLIPHFDSGGKVIGFYTLYTPRFMKKDGLSLESFKEKSVSENEQSWKRKLTQH
CHHEEEHHHHCCCCCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHC
SGGDATHQPARVSIDSSSRIILAIEQEEFRLFCQRILPVKPGQNATTYYEILIRMAEEES
CCCCCCCCCCEEEECCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
NLIPPGAFLPLVEKHNLMPRLDGWVVKKIIQYLSKKNVSTEISFCINLSNSTLKDPAFAD
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHH
HVQDLLKNAKVPPDKLCFEIEAFDVIANLQNTVLFVKKMQQLGCLVSLCSFNHDRTSFDL
HHHHHHHCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LKHIRADFLKIDGSIVCNILRDAEDLKKIENINKFARALKIGTIGELVETQAILEKLAEI
HHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GIDYAQGFAVGQPHPIETISRSTINLP
CCHHHCCCCCCCCCCHHHHCCCCCCCC
>Mature Secondary Structure
MYIHSKINTIKPFLPTAVTMVVAGLLIALDYSMLSIEPHIIVGLWVILVTAILITIIQIV
CEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
HAEKIFSIQFAQLTAQKERLANEIKYRIWAEKTSSENKAKLQIVDENFPVMLAYFNPEQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCEEEEEEECCCCEEEEEECCHHH
CRYHNRAFRQWFGLRSEQIENRFLHEFLSGSFYTHIKHEVGRVLSGETVQGQQIQKLINQ
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
SACLVTAHLIPHFDSGGKVIGFYTLYTPRFMKKDGLSLESFKEKSVSENEQSWKRKLTQH
CHHEEEHHHHCCCCCCCCEEEEEEECCCHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHC
SGGDATHQPARVSIDSSSRIILAIEQEEFRLFCQRILPVKPGQNATTYYEILIRMAEEES
CCCCCCCCCCEEEECCCCEEEEEEEHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCC
NLIPPGAFLPLVEKHNLMPRLDGWVVKKIIQYLSKKNVSTEISFCINLSNSTLKDPAFAD
CCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCHHHH
HVQDLLKNAKVPPDKLCFEIEAFDVIANLQNTVLFVKKMQQLGCLVSLCSFNHDRTSFDL
HHHHHHHCCCCCHHHHEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
LKHIRADFLKIDGSIVCNILRDAEDLKKIENINKFARALKIGTIGELVETQAILEKLAEI
HHHHHHHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GIDYAQGFAVGQPHPIETISRSTINLP
CCHHHCCCCCCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]