Click here to switch to the map view.

The map label for this gene is thcD [H]

Identifier: 114331173

GI number: 114331173

Start: 1225718

End: 1227241

Strand: Reverse

Name: thcD [H]

Synonym: Neut_1175

Alternate gene names: 114331173

Gene position: 1227241-1225718 (Counterclockwise)

Preceding gene: 114331175

Following gene: 114331164

Centisome position: 46.12

GC content: 50.2

Gene sequence:

>1524_bases
ATGTCTTCAGATACAACAGAAAACATGGGATTCGATCTGACCCAAGGGATTACCTTGGCTGATTTTGGTGATCAACAGCT
ATTGCGCGGTCAGGTCGGAGATGAATCCATAATACTGGTACGTACTGGTAATGAAATCATGGCAATTGGCGCCCATTGCA
CGCATTACGGTGGCCCTTTGGAAGAAGGTCTGGTTGTCGTCGGTGCAACAATACGTTGCCCTTGGCATCACGCTTGTTTT
TCACTGCGTAGCGGCGAGGCACTCGGTGCACCTGCTTTTGATCCACTGCCTTGCTGGAAAGTCGAGCGCGATGGGGAGCG
TATTTTTGTTCGTAATATAATCTCTCCTGCACCACAACCCGAACCGATAATTGCAGCAGCTGATCAACCTAAAAACATTG
TCATTGTGGGAGGTGGTGCAGCAGGATTTGCCTGTGCTGAAATGTTGCGACGACGCGGCTATCAAGGGCGCCTCACTATA
CTAAGTGAGGATACCGACGCACCTTGTGACCGTCCTAATTTGTCCAAAGATTATCTGGCCGGTACGGCGCCTGAAGAATG
GATGCCTTTGCGACCTGATGGCTTCTATAAAGACAATCACATTGATCTGCAACTCGGTACCATAGTGCAACATATTGATA
CCGCCAACCATCAGATAACTACAAAAGATGATCGCAGTATTTCATTCGATCGTCTTGTCCTGGCCACCGGAGCGGAGCCC
ATCCGGCTACCCATTCCCGGTCAGGATGGCGCACATGTATTTACCCTGCGTTCTTTTTCCGATAGCCGTCGCATCATTGA
ATATGCAAAAAACGCCAGGACAGTCGTAGTACTTGGCTCAGGATTCATTGGTCTGGAAGTGGCCGCCGCTTTGCGCGCAC
GCGGGCTCGCCGTGCATGTCGTGTCACTCGATCAGAGTCCGCTGCAAAATGTACTGGGTTCCGAGCTGGGTAATTTCATC
CGCAGCCTGCATGAAGCACACGGTGTAACATTTCACACGGAAACATCTTTGACGACCATTGAACCTGAATCAGTGACATT
AAGTAATGGCGACAAGCTGACTGCCGAACTCGTTATTCTTGGTGTGGGTGTGCGACCACGTACCACACTAGCTGAATTAG
CCGGCCTCAACGTTGACAAAGGTATCCTGGTGGACGAGTATCTGCAAACCAACGTGCCGGAAATTTATGCTGCAGGTGAT
GTGGCTCGCTGGCGCGATAAAGCAAGCGGTCTGGCGTTACGTATCGAACATTGGGTGGTAGCAGAACGACAAGGGCAAGT
GGCGGCTGAAAATCTACTGGGTGCCAATAAAAAATTTCAGGACGTGCCATTTTTCTGGAGCGTACATTACGACCTGACTA
TCAATTACGCTGGCCATGCCCAATCATGGGATGCGATTGAAATCGACGGTGATATAGCCGCTCGTGACTGTCTGGTGCGT
TATCAGAAAAATGGAGTAACCATTGCCGTTGCCGGGATCGGGCGTGATAAACAGATTCTGGAATTTGCAGCAGCAAACGC
ATAG

Upstream 100 bases:

>100_bases
ACTCTTCGATTTTTTCCGATCCGCCTTTTTACCTGTTAGATCTCATGTGCCATACTTGTCTGAGTTGTTTTACATCCACA
CTTTTAAAAGGAGGCTTGTG

Downstream 100 bases:

>100_bases
CCAGATACTTCGGCCACTGCACCATTCCATGTTAGTCAGAGTTAAATTATTTCTGTCTTTTCTGCTCACTACTCAGCTGG
TAGTCATGGGGATGCATTTT

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: Oxidized Ferredoxin; NADH; cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol; NAD [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 506

Protein sequence:

>507_residues
MSSDTTENMGFDLTQGITLADFGDQQLLRGQVGDESIILVRTGNEIMAIGAHCTHYGGPLEEGLVVVGATIRCPWHHACF
SLRSGEALGAPAFDPLPCWKVERDGERIFVRNIISPAPQPEPIIAAADQPKNIVIVGGGAAGFACAEMLRRRGYQGRLTI
LSEDTDAPCDRPNLSKDYLAGTAPEEWMPLRPDGFYKDNHIDLQLGTIVQHIDTANHQITTKDDRSISFDRLVLATGAEP
IRLPIPGQDGAHVFTLRSFSDSRRIIEYAKNARTVVVLGSGFIGLEVAAALRARGLAVHVVSLDQSPLQNVLGSELGNFI
RSLHEAHGVTFHTETSLTTIEPESVTLSNGDKLTAELVILGVGVRPRTTLAELAGLNVDKGILVDEYLQTNVPEIYAAGD
VARWRDKASGLALRIEHWVVAERQGQVAAENLLGANKKFQDVPFFWSVHYDLTINYAGHAQSWDAIEIDGDIAARDCLVR
YQKNGVTIAVAGIGRDKQILEFAAANA

Sequences:

>Translated_507_residues
MSSDTTENMGFDLTQGITLADFGDQQLLRGQVGDESIILVRTGNEIMAIGAHCTHYGGPLEEGLVVVGATIRCPWHHACF
SLRSGEALGAPAFDPLPCWKVERDGERIFVRNIISPAPQPEPIIAAADQPKNIVIVGGGAAGFACAEMLRRRGYQGRLTI
LSEDTDAPCDRPNLSKDYLAGTAPEEWMPLRPDGFYKDNHIDLQLGTIVQHIDTANHQITTKDDRSISFDRLVLATGAEP
IRLPIPGQDGAHVFTLRSFSDSRRIIEYAKNARTVVVLGSGFIGLEVAAALRARGLAVHVVSLDQSPLQNVLGSELGNFI
RSLHEAHGVTFHTETSLTTIEPESVTLSNGDKLTAELVILGVGVRPRTTLAELAGLNVDKGILVDEYLQTNVPEIYAAGD
VARWRDKASGLALRIEHWVVAERQGQVAAENLLGANKKFQDVPFFWSVHYDLTINYAGHAQSWDAIEIDGDIAARDCLVR
YQKNGVTIAVAGIGRDKQILEFAAANA
>Mature_506_residues
SSDTTENMGFDLTQGITLADFGDQQLLRGQVGDESIILVRTGNEIMAIGAHCTHYGGPLEEGLVVVGATIRCPWHHACFS
LRSGEALGAPAFDPLPCWKVERDGERIFVRNIISPAPQPEPIIAAADQPKNIVIVGGGAAGFACAEMLRRRGYQGRLTIL
SEDTDAPCDRPNLSKDYLAGTAPEEWMPLRPDGFYKDNHIDLQLGTIVQHIDTANHQITTKDDRSISFDRLVLATGAEPI
RLPIPGQDGAHVFTLRSFSDSRRIIEYAKNARTVVVLGSGFIGLEVAAALRARGLAVHVVSLDQSPLQNVLGSELGNFIR
SLHEAHGVTFHTETSLTTIEPESVTLSNGDKLTAELVILGVGVRPRTTLAELAGLNVDKGILVDEYLQTNVPEIYAAGDV
ARWRDKASGLALRIEHWVVAERQGQVAAENLLGANKKFQDVPFFWSVHYDLTINYAGHAQSWDAIEIDGDIAARDCLVRY
QKNGVTIAVAGIGRDKQILEFAAANA

Specific function: The degradation of the thiocarbamate herbicide EPTC by cytochrome CYP116 (thcB) requires the participation of a flavoprotein, rhodocoxin reductase, and an iron-sulfur protein, rhodocoxin, to mediate the transfer of electrons from NADH to P450 for oxygen a

COG id: COG0446

COG function: function code R; Uncharacterized NAD(FAD)-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=505, Percent_Identity=31.6831683168317, Blast_Score=281, Evalue=7e-76,
Organism=Homo sapiens, GI226437568, Length=505, Percent_Identity=31.6831683168317, Blast_Score=281, Evalue=9e-76,
Organism=Homo sapiens, GI65787454, Length=505, Percent_Identity=31.6831683168317, Blast_Score=281, Evalue=1e-75,
Organism=Homo sapiens, GI4757732, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI22202629, Length=337, Percent_Identity=26.4094955489614, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788892, Length=374, Percent_Identity=30.4812834224599, Blast_Score=149, Evalue=4e-37,
Organism=Escherichia coli, GI1789065, Length=337, Percent_Identity=28.1899109792285, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1789765, Length=318, Percent_Identity=28.9308176100629, Blast_Score=111, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17559934, Length=496, Percent_Identity=29.0322580645161, Blast_Score=195, Evalue=5e-50,
Organism=Caenorhabditis elegans, GI32564386, Length=376, Percent_Identity=23.4042553191489, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24639257, Length=487, Percent_Identity=33.0595482546201, Blast_Score=268, Evalue=9e-72,
Organism=Drosophila melanogaster, GI281359715, Length=487, Percent_Identity=33.0595482546201, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI281359713, Length=487, Percent_Identity=33.0595482546201, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24639250, Length=487, Percent_Identity=33.0595482546201, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI18543267, Length=487, Percent_Identity=33.0595482546201, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24639252, Length=487, Percent_Identity=33.0595482546201, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24585130, Length=496, Percent_Identity=31.0483870967742, Blast_Score=230, Evalue=2e-60,
Organism=Drosophila melanogaster, GI28573993, Length=366, Percent_Identity=27.8688524590164, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24581020, Length=366, Percent_Identity=27.8688524590164, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.18.1.3 [C]

Molecular weight: Translated: 55020; Mature: 54889

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDTTENMGFDLTQGITLADFGDQQLLRGQVGDESIILVRTGNEIMAIGAHCTHYGGPL
CCCCCCCCCCCCHHCCEEEECCCCHHHHHCCCCCCEEEEEEECCEEEEEECCCCCCCCCH
EEGLVVVGATIRCPWHHACFSLRSGEALGAPAFDPLPCWKVERDGERIFVRNIISPAPQP
HCCEEEEEEEEECCHHHHHEEECCCCCCCCCCCCCCCCEEECCCCCEEEEEHHCCCCCCC
EPIIAAADQPKNIVIVGGGAAGFACAEMLRRRGYQGRLTILSEDTDAPCDRPNLSKDYLA
CCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHC
GTAPEEWMPLRPDGFYKDNHIDLQLGTIVQHIDTANHQITTKDDRSISFDRLVLATGAEP
CCCCHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCC
IRLPIPGQDGAHVFTLRSFSDSRRIIEYAKNARTVVVLGSGFIGLEVAAALRARGLAVHV
EEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCEEEEE
VSLDQSPLQNVLGSELGNFIRSLHEAHGVTFHTETSLTTIEPESVTLSNGDKLTAELVIL
EECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCEEEECCCCEEEEEEEEE
GVGVRPRTTLAELAGLNVDKGILVDEYLQTNVPEIYAAGDVARWRDKASGLALRIEHWVV
ECCCCCCHHHHHHHCCCCCCCCEEHHHHHCCCCCEEECCCHHHHHHCCCCEEEEEEEEEE
AERQGQVAAENLLGANKKFQDVPFFWSVHYDLTINYAGHAQSWDAIEIDGDIAARDCLVR
ECCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEECCCHHHHHHHEE
YQKNGVTIAVAGIGRDKQILEFAAANA
EECCCEEEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
SSDTTENMGFDLTQGITLADFGDQQLLRGQVGDESIILVRTGNEIMAIGAHCTHYGGPL
CCCCCCCCCCCHHCCEEEECCCCHHHHHCCCCCCEEEEEEECCEEEEEECCCCCCCCCH
EEGLVVVGATIRCPWHHACFSLRSGEALGAPAFDPLPCWKVERDGERIFVRNIISPAPQP
HCCEEEEEEEEECCHHHHHEEECCCCCCCCCCCCCCCCEEECCCCCEEEEEHHCCCCCCC
EPIIAAADQPKNIVIVGGGAAGFACAEMLRRRGYQGRLTILSEDTDAPCDRPNLSKDYLA
CCEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCHHHHC
GTAPEEWMPLRPDGFYKDNHIDLQLGTIVQHIDTANHQITTKDDRSISFDRLVLATGAEP
CCCCHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCCCCEEECCCCCCCCCCEEEEECCCCC
IRLPIPGQDGAHVFTLRSFSDSRRIIEYAKNARTVVVLGSGFIGLEVAAALRARGLAVHV
EEEECCCCCCCEEEEEECCCCCHHHHHHHCCCCEEEEEECCCHHHHHHHHHHHCCEEEEE
VSLDQSPLQNVLGSELGNFIRSLHEAHGVTFHTETSLTTIEPESVTLSNGDKLTAELVIL
EECCCHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEECCCEEEECCCCEEEEEEEEE
GVGVRPRTTLAELAGLNVDKGILVDEYLQTNVPEIYAAGDVARWRDKASGLALRIEHWVV
ECCCCCCHHHHHHHCCCCCCCCEEHHHHHCCCCCEEECCCHHHHHHCCCCEEEEEEEEEE
AERQGQVAAENLLGANKKFQDVPFFWSVHYDLTINYAGHAQSWDAIEIDGDIAARDCLVR
ECCCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEEEECCCCCCCCEEEECCCHHHHHHHEE
YQKNGVTIAVAGIGRDKQILEFAAANA
EECCCEEEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Reduced Ferredoxin; NAD(+); 3-phenylpropionate; NADH; Proton; O2 [C]

Specific reaction: Reduced Ferredoxin + NAD(+) = Oxidized Ferredoxin + NADH. 3-phenylpropionate + NADH + Proton + O2 = cis-3-(carboxyethyl)-3,5-cyclohexadiene-1,2-diol + NAD [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7836301 [H]