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The map label for this gene is rsmE [H]

Identifier: 114331048

GI number: 114331048

Start: 1086303

End: 1087064

Strand: Reverse

Name: rsmE [H]

Synonym: Neut_1049

Alternate gene names: 114331048

Gene position: 1087064-1086303 (Counterclockwise)

Preceding gene: 114331051

Following gene: 114331047

Centisome position: 40.85

GC content: 47.77

Gene sequence:

>762_bases
ATGACAGCACGCTTTTTTCATACTCTCCCTATTCATCCCGATGCCAAACAGGTTACGCTAAACAACGATACCAGTCATCA
CGCAGTTCAGGTACTCCGATTAAAACAGGGAGATCCGGTGATCCTGTTCGATGGTACAGGAGGTGAATTTTCCGGTTGCC
TTGTACAAGTCAGAAAATCTGCTTGTGTCGTTAAAATCGAACAGCACCTGCCAGTTGAGCGTGAATCCCCCTTAATGCTT
ACGCTGGCACAAGCTGTCTGTACCAATGAAAAAATGGACTGGATCATTCAGAAAGCTGTTGAACAAGGCGTCATTTATAT
ACAACCTTTGATTACTAACCGTACACTCATCCGACTCACAGGAGAACGTGCCGATAAACGCTGGCAACACTGGCAAAAAA
TCATTATTTCTGCCTGTGAACAATGTGGACGGAACAGAATTCCCCAGCTGTTACCACTTATGTCCCTATCTCATTGGTTA
GAACAGAAACTGTTGCAAAAACAGGAAAATAGTAATCTATCCGGGCGCGATATAATACTCTCCCCTGTCGCTCGTCTGCG
TTTGGCCGAGTTGTCTCCTCCCGAATCCGGGGAGTGCATTACATTACTGACAGGCCCGGAGGGAGGATTTACTGAAGAAG
AAACCGACGCCATGCTTCACGCCGGATTTATACCTATCTGCCTGGGCACTCGTATTCTGCGCACCGAAACCGCTGCATTG
GCAGCCATTGCCGCCGCCCAGGTGCTCTGGGGTGATTATTAA

Upstream 100 bases:

>100_bases
CATCTAGCCACTCCTTTTTCATTAATCAATTTCATTCTTACCTATACTATCATAAAAATATTTTGTTACTTTTTCTCTCA
AGATATCAATTCTTTTTTAT

Downstream 100 bases:

>100_bases
TAGTGCTTCTACCTTGATCAGCCATGCAATCGGACATCGAATTTGTTACCTGGTTTCGCTCCGTTACTCCGCATATCCAC
ACCAGTCGTGGAAAAATCTT

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MTARFFHTLPIHPDAKQVTLNNDTSHHAVQVLRLKQGDPVILFDGTGGEFSGCLVQVRKSACVVKIEQHLPVERESPLML
TLAQAVCTNEKMDWIIQKAVEQGVIYIQPLITNRTLIRLTGERADKRWQHWQKIIISACEQCGRNRIPQLLPLMSLSHWL
EQKLLQKQENSNLSGRDIILSPVARLRLAELSPPESGECITLLTGPEGGFTEEETDAMLHAGFIPICLGTRILRTETAAL
AAIAAAQVLWGDY

Sequences:

>Translated_253_residues
MTARFFHTLPIHPDAKQVTLNNDTSHHAVQVLRLKQGDPVILFDGTGGEFSGCLVQVRKSACVVKIEQHLPVERESPLML
TLAQAVCTNEKMDWIIQKAVEQGVIYIQPLITNRTLIRLTGERADKRWQHWQKIIISACEQCGRNRIPQLLPLMSLSHWL
EQKLLQKQENSNLSGRDIILSPVARLRLAELSPPESGECITLLTGPEGGFTEEETDAMLHAGFIPICLGTRILRTETAAL
AAIAAAQVLWGDY
>Mature_252_residues
TARFFHTLPIHPDAKQVTLNNDTSHHAVQVLRLKQGDPVILFDGTGGEFSGCLVQVRKSACVVKIEQHLPVERESPLMLT
LAQAVCTNEKMDWIIQKAVEQGVIYIQPLITNRTLIRLTGERADKRWQHWQKIIISACEQCGRNRIPQLLPLMSLSHWLE
QKLLQKQENSNLSGRDIILSPVARLRLAELSPPESGECITLLTGPEGGFTEEETDAMLHAGFIPICLGTRILRTETAALA
AIAAAQVLWGDY

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=249, Percent_Identity=38.5542168674699, Blast_Score=164, Evalue=4e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 28228; Mature: 28097

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARFFHTLPIHPDAKQVTLNNDTSHHAVQVLRLKQGDPVILFDGTGGEFSGCLVQVRKS
CCCEEEEECCCCCCCCEEEECCCCCHHHEEEEEECCCCCEEEEECCCCCHHHHHHHHHHH
ACVVKIEQHLPVERESPLMLTLAQAVCTNEKMDWIIQKAVEQGVIYIQPLITNRTLIRLT
HHEEEEHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEEECCCEEEEEE
GERADKRWQHWQKIIISACEQCGRNRIPQLLPLMSLSHWLEQKLLQKQENSNLSGRDIIL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
SPVARLRLAELSPPESGECITLLTGPEGGFTEEETDAMLHAGFIPICLGTRILRTETAAL
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHCCHHHHHHHHHH
AAIAAAQVLWGDY
HHHHHHHHHHCCC
>Mature Secondary Structure 
TARFFHTLPIHPDAKQVTLNNDTSHHAVQVLRLKQGDPVILFDGTGGEFSGCLVQVRKS
CCEEEEECCCCCCCCEEEECCCCCHHHEEEEEECCCCCEEEEECCCCCHHHHHHHHHHH
ACVVKIEQHLPVERESPLMLTLAQAVCTNEKMDWIIQKAVEQGVIYIQPLITNRTLIRLT
HHEEEEHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCEEEEEEEECCCEEEEEE
GERADKRWQHWQKIIISACEQCGRNRIPQLLPLMSLSHWLEQKLLQKQENSNLSGRDIIL
CCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
SPVARLRLAELSPPESGECITLLTGPEGGFTEEETDAMLHAGFIPICLGTRILRTETAAL
HHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCHHHHHHHHCCCHHHHHCCHHHHHHHHHH
AAIAAAQVLWGDY
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8332061 [H]