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The map label for this gene is cysU [H]

Identifier: 114331026

GI number: 114331026

Start: 1059390

End: 1060223

Strand: Reverse

Name: cysU [H]

Synonym: Neut_1026

Alternate gene names: 114331026

Gene position: 1060223-1059390 (Counterclockwise)

Preceding gene: 114331027

Following gene: 114331025

Centisome position: 39.84

GC content: 51.2

Gene sequence:

>834_bases
TTGAGCACTTTCAAACAATACAGTGTGTTACCCGGGTTCAATCTGGCACTTGGATTCACCCTGCTCTACCTGAGTCTGGT
CGTGCTAATTCCCCTGTCGGCAGCATTCATCCACAGCGCGAAACTGACCTGGCCGGAATTCTGGAGTACCGTGACCGCAC
CCCGAGTTATCGCATCCTATCGACTGACATTTGGAGCCTCATTTGCAGCCGCAGTGGTCAATGCATTTTTCGGATTGCTG
GTAGCCTGGGTGCTGGTACGTTACCCGTTTCCGGGCAAGCGTCTGGTCGATGCCCTGATCGATCTGCCTTTCGCACTGCC
CACCTCCGTTGCCGGCATCACACTCACCGCAATTTACGCGGGGAACGGCTGGATCGGACAGTACCTGGAACCACTGGGAA
TCAAGGTTGCATTCACACCCATCGGCGTATTTGTTGCCCTGACATTCATCGGATTGCCGTTCGTTGTGCGTACCGTTCAG
CCCGTACTGGAAGATATCGAAAAAGAACTGGAAGAAGCTGCAGCCATGTTAGGTGCAACTCGCTGGCAAATTTTCCGGCG
CATTATTTTCCCCACAATTCTGCCCGCACTGACCACTGGCTTTGCATTGGCGTTTGCCCGCGCTATCGGGGAATATGGAT
CAGTGATTTTTATCGCCGGCAATATCCCGATGATTTCGGAAATCACGCCGTTACTGATTATCACCAAACTGGAACAATAT
GATTACACCGGTGCAACTGCTATTGCTGTCGTGATGTTGGTGATTTCATTCATTCTTTTGCTGATCATCAACCTGTTGCA
ATGGTGGGTTCACCATCGCGGCATAAAAGTATAA

Upstream 100 bases:

>100_bases
ATCCGCAGGGCGGTTTTTAGGACAGGCAGCATAACTCGCAATATGATCTCGGCAGCCATCTAGCAAAGATTTTCACAATC
GAATAACAGGAGCTTGAATT

Downstream 100 bases:

>100_bases
AGGAATCATGACTACCATCCCATTACTTCAAGCCTCTTCGGCACCGCACAAGATACTGGTTACACAGGAATCACCATGGG
CACGCTGGATACTCACCCTG

Product: sulfate ABC transporter, inner membrane subunit CysT

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MSTFKQYSVLPGFNLALGFTLLYLSLVVLIPLSAAFIHSAKLTWPEFWSTVTAPRVIASYRLTFGASFAAAVVNAFFGLL
VAWVLVRYPFPGKRLVDALIDLPFALPTSVAGITLTAIYAGNGWIGQYLEPLGIKVAFTPIGVFVALTFIGLPFVVRTVQ
PVLEDIEKELEEAAAMLGATRWQIFRRIIFPTILPALTTGFALAFARAIGEYGSVIFIAGNIPMISEITPLLIITKLEQY
DYTGATAIAVVMLVISFILLLIINLLQWWVHHRGIKV

Sequences:

>Translated_277_residues
MSTFKQYSVLPGFNLALGFTLLYLSLVVLIPLSAAFIHSAKLTWPEFWSTVTAPRVIASYRLTFGASFAAAVVNAFFGLL
VAWVLVRYPFPGKRLVDALIDLPFALPTSVAGITLTAIYAGNGWIGQYLEPLGIKVAFTPIGVFVALTFIGLPFVVRTVQ
PVLEDIEKELEEAAAMLGATRWQIFRRIIFPTILPALTTGFALAFARAIGEYGSVIFIAGNIPMISEITPLLIITKLEQY
DYTGATAIAVVMLVISFILLLIINLLQWWVHHRGIKV
>Mature_276_residues
STFKQYSVLPGFNLALGFTLLYLSLVVLIPLSAAFIHSAKLTWPEFWSTVTAPRVIASYRLTFGASFAAAVVNAFFGLLV
AWVLVRYPFPGKRLVDALIDLPFALPTSVAGITLTAIYAGNGWIGQYLEPLGIKVAFTPIGVFVALTFIGLPFVVRTVQP
VLEDIEKELEEAAAMLGATRWQIFRRIIFPTILPALTTGFALAFARAIGEYGSVIFIAGNIPMISEITPLLIITKLEQYD
YTGATAIAVVMLVISFILLLIINLLQWWVHHRGIKV

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=259, Percent_Identity=51.7374517374517, Blast_Score=255, Evalue=2e-69,
Organism=Escherichia coli, GI87082099, Length=275, Percent_Identity=31.2727272727273, Blast_Score=144, Evalue=7e-36,
Organism=Escherichia coli, GI1786980, Length=199, Percent_Identity=36.1809045226131, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1786254, Length=206, Percent_Identity=27.6699029126214, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787368, Length=212, Percent_Identity=31.1320754716981, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1787369, Length=136, Percent_Identity=32.3529411764706, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30407; Mature: 30275

Theoretical pI: Translated: 9.49; Mature: 9.49

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKQYSVLPGFNLALGFTLLYLSLVVLIPLSAAFIHSAKLTWPEFWSTVTAPRVIASY
CCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RLTFGASFAAAVVNAFFGLLVAWVLVRYPFPGKRLVDALIDLPFALPTSVAGITLTAIYA
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHEEEEEEE
GNGWIGQYLEPLGIKVAFTPIGVFVALTFIGLPFVVRTVQPVLEDIEKELEEAAAMLGAT
CCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWQIFRRIIFPTILPALTTGFALAFARAIGEYGSVIFIAGNIPMISEITPLLIITKLEQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHHHHHC
DYTGATAIAVVMLVISFILLLIINLLQWWVHHRGIKV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
STFKQYSVLPGFNLALGFTLLYLSLVVLIPLSAAFIHSAKLTWPEFWSTVTAPRVIASY
CCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
RLTFGASFAAAVVNAFFGLLVAWVLVRYPFPGKRLVDALIDLPFALPTSVAGITLTAIYA
HHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHEEEEEEE
GNGWIGQYLEPLGIKVAFTPIGVFVALTFIGLPFVVRTVQPVLEDIEKELEEAAAMLGAT
CCCCHHHHHHHHCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RWQIFRRIIFPTILPALTTGFALAFARAIGEYGSVIFIAGNIPMISEITPLLIITKLEQY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCHHHCCHHHHHHHHHHC
DYTGATAIAVVMLVISFILLLIINLLQWWVHHRGIKV
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]