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The map label for this gene is cysW [H]

Identifier: 114331025

GI number: 114331025

Start: 1058513

End: 1059382

Strand: Reverse

Name: cysW [H]

Synonym: Neut_1025

Alternate gene names: 114331025

Gene position: 1059382-1058513 (Counterclockwise)

Preceding gene: 114331026

Following gene: 114331024

Centisome position: 39.81

GC content: 50.46

Gene sequence:

>870_bases
ATGACTACCATCCCATTACTTCAAGCCTCTTCGGCACCGCACAAGATACTGGTTACACAGGAATCACCATGGGCACGCTG
GATACTCACCCTGCTGGCGCTCTCCTTCCTATCCCTTTTTCTTTTACTACCGCTGGTAGCAGTCTTTTTTGAAGCGCTGA
AAAAAGGATGGGATGTTTACATCGCTGCTATCACCGAACCAGATGCTCTCGCTGCCATCCAGCTCACACTGACCGCTGCA
GCCATTGCCATACCACTTAATCTGGCTTTCGGCATTGCGGCTGCCTGGGCAATTGCCAAATTCGAGTTTCGCGGCAAAAG
TATTTTGATCTCCCTGATTGATCTGCCCTTCTCCGTCTCTCCCGTCGTTGCCGGATTAATTTACGTACTAATATTCGGTC
TGCAGGGCTGGATGGGACCGTGGTTGAGAGAGCACGATATTTCTATCATTTTTGCAGTGCCCGGCATTGTTCTGGCCACC
ATCTTTGTGACTGTGCCATTTATCGCACGCGAACTGATTCCACTGATGCAAGCCCAAGGAACAGAGGAGGAAGAAGCGGC
CATCATTCTCGGGGCGAATGGCTGGCAGACGCTCTGGTATATCACTCTTCCCAACATCAAGTGGGGATTGCTGTATGGCA
CCATCCTTTGCAATGCAAGGGCAATGGGCGAGTTCGGGGCGGTATCGGTTGTTTCTGGGCATATTCGCGGCCTGACCAAT
ACACTTTCCCTGCACGTCGAAATTCTTTACAACGAATACAACTCTGCTGCTGCTTTTGCAGTGGCCTCCCTGCTGGCGTT
ACTGGCACTGATTACGCTGGCACTAAAAACATTTATTGAATCCAGAGTTGCGCAGAAAACATCCAGATAA

Upstream 100 bases:

>100_bases
ATTGCTGTCGTGATGTTGGTGATTTCATTCATTCTTTTGCTGATCATCAACCTGTTGCAATGGTGGGTTCACCATCGCGG
CATAAAAGTATAAAGGAATC

Downstream 100 bases:

>100_bases
GGAAATTACACATGAGTATCGAGATACGCAATCTTTCCAGGCAGTTTGGCTCTTTTGTTGCCCTGCATAATATTAATTTA
CAAGTTAATCCAGGCGAACT

Product: sulfate ABC transporter, inner membrane subunit CysW

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTTIPLLQASSAPHKILVTQESPWARWILTLLALSFLSLFLLLPLVAVFFEALKKGWDVYIAAITEPDALAAIQLTLTAA
AIAIPLNLAFGIAAAWAIAKFEFRGKSILISLIDLPFSVSPVVAGLIYVLIFGLQGWMGPWLREHDISIIFAVPGIVLAT
IFVTVPFIARELIPLMQAQGTEEEEAAIILGANGWQTLWYITLPNIKWGLLYGTILCNARAMGEFGAVSVVSGHIRGLTN
TLSLHVEILYNEYNSAAAFAVASLLALLALITLALKTFIESRVAQKTSR

Sequences:

>Translated_289_residues
MTTIPLLQASSAPHKILVTQESPWARWILTLLALSFLSLFLLLPLVAVFFEALKKGWDVYIAAITEPDALAAIQLTLTAA
AIAIPLNLAFGIAAAWAIAKFEFRGKSILISLIDLPFSVSPVVAGLIYVLIFGLQGWMGPWLREHDISIIFAVPGIVLAT
IFVTVPFIARELIPLMQAQGTEEEEAAIILGANGWQTLWYITLPNIKWGLLYGTILCNARAMGEFGAVSVVSGHIRGLTN
TLSLHVEILYNEYNSAAAFAVASLLALLALITLALKTFIESRVAQKTSR
>Mature_288_residues
TTIPLLQASSAPHKILVTQESPWARWILTLLALSFLSLFLLLPLVAVFFEALKKGWDVYIAAITEPDALAAIQLTLTAAA
IAIPLNLAFGIAAAWAIAKFEFRGKSILISLIDLPFSVSPVVAGLIYVLIFGLQGWMGPWLREHDISIIFAVPGIVLATI
FVTVPFIARELIPLMQAQGTEEEEAAIILGANGWQTLWYITLPNIKWGLLYGTILCNARAMGEFGAVSVVSGHIRGLTNT
LSLHVEILYNEYNSAAAFAVASLLALLALITLALKTFIESRVAQKTSR

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=266, Percent_Identity=50, Blast_Score=282, Evalue=2e-77,
Organism=Escherichia coli, GI1788764, Length=233, Percent_Identity=34.7639484978541, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1786980, Length=213, Percent_Identity=30.9859154929577, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31369; Mature: 31237

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTIPLLQASSAPHKILVTQESPWARWILTLLALSFLSLFLLLPLVAVFFEALKKGWDVY
CCCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IAAITEPDALAAIQLTLTAAAIAIPLNLAFGIAAAWAIAKFEFRGKSILISLIDLPFSVS
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHH
PVVAGLIYVLIFGLQGWMGPWLREHDISIIFAVPGIVLATIFVTVPFIARELIPLMQAQG
HHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TEEEEAAIILGANGWQTLWYITLPNIKWGLLYGTILCNARAMGEFGAVSVVSGHIRGLTN
CCCCCCEEEEECCCCEEEEEEEECCCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHH
TLSLHVEILYNEYNSAAAFAVASLLALLALITLALKTFIESRVAQKTSR
HHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TTIPLLQASSAPHKILVTQESPWARWILTLLALSFLSLFLLLPLVAVFFEALKKGWDVY
CCCCCEECCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
IAAITEPDALAAIQLTLTAAAIAIPLNLAFGIAAAWAIAKFEFRGKSILISLIDLPFSVS
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEEEEECCCCCCHH
PVVAGLIYVLIFGLQGWMGPWLREHDISIIFAVPGIVLATIFVTVPFIARELIPLMQAQG
HHHHHHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TEEEEAAIILGANGWQTLWYITLPNIKWGLLYGTILCNARAMGEFGAVSVVSGHIRGLTN
CCCCCCEEEEECCCCEEEEEEEECCCCHHHHHHHHHHCCHHCCCCCCHHHHHHHHHHHHH
TLSLHVEILYNEYNSAAAFAVASLLALLALITLALKTFIESRVAQKTSR
HHEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]