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The map label for this gene is pyk [H]

Identifier: 114330982

GI number: 114330982

Start: 1011147

End: 1012598

Strand: Reverse

Name: pyk [H]

Synonym: Neut_0979

Alternate gene names: 114330982

Gene position: 1012598-1011147 (Counterclockwise)

Preceding gene: 114330983

Following gene: 114330981

Centisome position: 38.05

GC content: 62.6

Gene sequence:

>1452_bases
ATGGACGGCCCCGCACAGGCAGAGATGCCCCAACTCCCTTGGCATCGAACAAAGATCATCTGCACCCTGGGTCCGGCGAC
CGATCAACCGGGCGTGTTGGCGCGACTGATCGGCGCGGGGATGATGGATGTCGCCCGCTTCAATCTGTCGCACGGCGACC
ACGCCAGCCATGCCCGCCGGATTCAGCAGGTGCGTCAATTGGCGCAACAAGCGGGACGGTTCATCGCCGTCCTGATGGAC
CTGCCAGGACCGAAGTTCCGGCTGGGGGAGTTGTCCAACGGTGCGCGCGAACTGCATCTGGGCGCGGATGTGATCCTGGC
GCTCGAGGCCGATCCTCCAGATGGGCTACCGGTAAAGCACCCGGCGTTACTGCAGGCGCTGCGTGTGGGTGAGTCAGTAT
ATCTCGCGGACGGCGCGATCAGGCTTGAGGTTAAAATCGCTGGCGCGGAGCGGGTTGTCTGCCAGGTGCTCGTCAGCGGG
ACGGTCACATCGGGTTCGGGCATCAACGTGCCAGAATCCAAACGCTCGGTGCTCATACCGACAGACGATGACCGCCGGCA
CTTGGTCTTTGCGCTGGAGCAACAGGCGGAGTGGATCGGCGTGTCGTTTGTCCAGTCCGCTGATGACCTGATCCGCGTCC
GGACACTGCTGCCGCCCGGCCAGCAGCCTTTGCTGATGGCCAAGATCGAAAAACGCCAAGCTCTGGTCGACCTCGATGCG
ATCATGGCGACGTCCGATGGTGTGATGGTGGCTCGCGGGGATTTGGGGGTGGAGACCGATCTGGCTGAGATACCCCTGGT
CCAGAAACGGATCATCGCCTTGGCCAATGCCCAGGCACGCCCGGTGATCACCGCCACCCAGATGCTCGAATCCATGGTTA
CTCAGGAACAACCAACACGTGCCGAGGTGACGGATGTCGCCAATGCTATGCTTGATGGGACGGACGGCGTGATGCTGTCC
GCCGAGACCGCCATCGGACGCTTTCCAGTGGCTGCTGCCGAAATTCTGCAGCGGGTACTGACGGCCACTGAAACCGAGTA
TGCAGTACGCGTTGCACGTGACCGGCTACGGGCAAGTGAGTCGACCCCAGCGACAAACCCCATCAGCCTGGTTGCCTGCC
AGCTTGCGGCCCGTCTCAACGCTAAAGCCATCATTGCCCCCGTCAGGGATATAGCGACCGCGCTGGGGATTGCCCGCTTC
CGGCCCGCGGCGCCGCTAGTGGTTATGGCTGACTCTGAAGACTTGTGCCGCCGTCTGGCGGTGGTCTGGGGCGTATCACC
GTTGTTAGCCGTGGCGGAGTTTGAACCGCAATCGTGCCTGTACTTGGCCAGCCACTGGCTGTGCGCGCAAGGGCTTGCTC
AGCCAGGCGATCCCGTCGTCATGTTGTCCACCTCCGGGGCAGCACAGGGCGCGGCCGATACACTACAGGTAATGCGCGTG
GACAACAGTTAA

Upstream 100 bases:

>100_bases
ATGACGGGGCGGCGCGTGATAATTCTTGGCGTCCTTGGCACCTCAACACACTAAGCGTACAGTGGCTCATGAGATGGTCC
GCATTCCCGAATCCAAGCTG

Downstream 100 bases:

>100_bases
CGATCCTGCTACCAAGTGATGCGCTGGCCGAGCCCGTGTTTGGCATTATCAAACAGATATTAGGTGTTTAGTCCCACGGT
GTGCTGGTGTAACATATAAC

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 483; Mature: 483

Protein sequence:

>483_residues
MDGPAQAEMPQLPWHRTKIICTLGPATDQPGVLARLIGAGMMDVARFNLSHGDHASHARRIQQVRQLAQQAGRFIAVLMD
LPGPKFRLGELSNGARELHLGADVILALEADPPDGLPVKHPALLQALRVGESVYLADGAIRLEVKIAGAERVVCQVLVSG
TVTSGSGINVPESKRSVLIPTDDDRRHLVFALEQQAEWIGVSFVQSADDLIRVRTLLPPGQQPLLMAKIEKRQALVDLDA
IMATSDGVMVARGDLGVETDLAEIPLVQKRIIALANAQARPVITATQMLESMVTQEQPTRAEVTDVANAMLDGTDGVMLS
AETAIGRFPVAAAEILQRVLTATETEYAVRVARDRLRASESTPATNPISLVACQLAARLNAKAIIAPVRDIATALGIARF
RPAAPLVVMADSEDLCRRLAVVWGVSPLLAVAEFEPQSCLYLASHWLCAQGLAQPGDPVVMLSTSGAAQGAADTLQVMRV
DNS

Sequences:

>Translated_483_residues
MDGPAQAEMPQLPWHRTKIICTLGPATDQPGVLARLIGAGMMDVARFNLSHGDHASHARRIQQVRQLAQQAGRFIAVLMD
LPGPKFRLGELSNGARELHLGADVILALEADPPDGLPVKHPALLQALRVGESVYLADGAIRLEVKIAGAERVVCQVLVSG
TVTSGSGINVPESKRSVLIPTDDDRRHLVFALEQQAEWIGVSFVQSADDLIRVRTLLPPGQQPLLMAKIEKRQALVDLDA
IMATSDGVMVARGDLGVETDLAEIPLVQKRIIALANAQARPVITATQMLESMVTQEQPTRAEVTDVANAMLDGTDGVMLS
AETAIGRFPVAAAEILQRVLTATETEYAVRVARDRLRASESTPATNPISLVACQLAARLNAKAIIAPVRDIATALGIARF
RPAAPLVVMADSEDLCRRLAVVWGVSPLLAVAEFEPQSCLYLASHWLCAQGLAQPGDPVVMLSTSGAAQGAADTLQVMRV
DNS
>Mature_483_residues
MDGPAQAEMPQLPWHRTKIICTLGPATDQPGVLARLIGAGMMDVARFNLSHGDHASHARRIQQVRQLAQQAGRFIAVLMD
LPGPKFRLGELSNGARELHLGADVILALEADPPDGLPVKHPALLQALRVGESVYLADGAIRLEVKIAGAERVVCQVLVSG
TVTSGSGINVPESKRSVLIPTDDDRRHLVFALEQQAEWIGVSFVQSADDLIRVRTLLPPGQQPLLMAKIEKRQALVDLDA
IMATSDGVMVARGDLGVETDLAEIPLVQKRIIALANAQARPVITATQMLESMVTQEQPTRAEVTDVANAMLDGTDGVMLS
AETAIGRFPVAAAEILQRVLTATETEYAVRVARDRLRASESTPATNPISLVACQLAARLNAKAIIAPVRDIATALGIARF
RPAAPLVVMADSEDLCRRLAVVWGVSPLLAVAEFEPQSCLYLASHWLCAQGLAQPGDPVVMLSTSGAAQGAADTLQVMRV
DNS

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=496, Percent_Identity=33.2661290322581, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI33286420, Length=496, Percent_Identity=33.2661290322581, Blast_Score=242, Evalue=5e-64,
Organism=Homo sapiens, GI33286418, Length=497, Percent_Identity=33.4004024144869, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI10835121, Length=432, Percent_Identity=36.8055555555556, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI32967597, Length=432, Percent_Identity=36.8055555555556, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI310128732, Length=293, Percent_Identity=33.7883959044369, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128730, Length=293, Percent_Identity=33.7883959044369, Blast_Score=155, Evalue=1e-37,
Organism=Homo sapiens, GI310128736, Length=241, Percent_Identity=31.1203319502075, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128734, Length=241, Percent_Identity=31.1203319502075, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=30.2325581395349, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI1787965, Length=477, Percent_Identity=35.0104821802935, Blast_Score=274, Evalue=1e-74,
Organism=Escherichia coli, GI1788160, Length=480, Percent_Identity=34.375, Blast_Score=220, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17544584, Length=490, Percent_Identity=32.6530612244898, Blast_Score=253, Evalue=1e-67,
Organism=Caenorhabditis elegans, GI17506831, Length=490, Percent_Identity=34.8979591836735, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=34.8979591836735, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI17506829, Length=490, Percent_Identity=34.8979591836735, Blast_Score=229, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI71984413, Length=490, Percent_Identity=34.8979591836735, Blast_Score=229, Evalue=2e-60,
Organism=Saccharomyces cerevisiae, GI6319279, Length=485, Percent_Identity=31.340206185567, Blast_Score=223, Evalue=8e-59,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=31.006160164271, Blast_Score=212, Evalue=8e-56,
Organism=Drosophila melanogaster, GI24648966, Length=407, Percent_Identity=35.8722358722359, Blast_Score=235, Evalue=5e-62,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=31.4285714285714, Blast_Score=233, Evalue=2e-61,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=31.4285714285714, Blast_Score=233, Evalue=3e-61,
Organism=Drosophila melanogaster, GI24581235, Length=492, Percent_Identity=27.6422764227642, Blast_Score=183, Evalue=3e-46,
Organism=Drosophila melanogaster, GI24646914, Length=255, Percent_Identity=34.9019607843137, Blast_Score=150, Evalue=1e-36,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 51531; Mature: 51531

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGPAQAEMPQLPWHRTKIICTLGPATDQPGVLARLIGAGMMDVARFNLSHGDHASHARR
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHCCCCCCCHHHHHH
IQQVRQLAQQAGRFIAVLMDLPGPKFRLGELSNGARELHLGADVILALEADPPDGLPVKH
HHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCEEEEECCEEEEEEECCCCCCCCCCC
PALLQALRVGESVYLADGAIRLEVKIAGAERVVCQVLVSGTVTSGSGINVPESKRSVLIP
HHHHHHHHCCCEEEEECCEEEEEEEECCHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEC
TDDDRRHLVFALEQQAEWIGVSFVQSADDLIRVRTLLPPGQQPLLMAKIEKRQALVDLDA
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
IMATSDGVMVARGDLGVETDLAEIPLVQKRIIALANAQARPVITATQMLESMVTQEQPTR
HHCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCH
AEVTDVANAMLDGTDGVMLSAETAIGRFPVAAAEILQRVLTATETEYAVRVARDRLRASE
HHHHHHHHHHHCCCCCEEEEECHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
STPATNPISLVACQLAARLNAKAIIAPVRDIATALGIARFRPAAPLVVMADSEDLCRRLA
CCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHH
VVWGVSPLLAVAEFEPQSCLYLASHWLCAQGLAQPGDPVVMLSTSGAAQGAADTLQVMRV
HHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHEEEEEEE
DNS
CCC
>Mature Secondary Structure
MDGPAQAEMPQLPWHRTKIICTLGPATDQPGVLARLIGAGMMDVARFNLSHGDHASHARR
CCCCCCCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHCCCCCCCHHHHHH
IQQVRQLAQQAGRFIAVLMDLPGPKFRLGELSNGARELHLGADVILALEADPPDGLPVKH
HHHHHHHHHHCCCEEEEEEECCCCCEEECCCCCCCEEEEECCEEEEEEECCCCCCCCCCC
PALLQALRVGESVYLADGAIRLEVKIAGAERVVCQVLVSGTVTSGSGINVPESKRSVLIP
HHHHHHHHCCCEEEEECCEEEEEEEECCHHHHHHHHHHHCCEECCCCCCCCCCCCEEEEC
TDDDRRHLVFALEQQAEWIGVSFVQSADDLIRVRTLLPPGQQPLLMAKIEKRQALVDLDA
CCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHHHH
IMATSDGVMVARGDLGVETDLAEIPLVQKRIIALANAQARPVITATQMLESMVTQEQPTR
HHCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCH
AEVTDVANAMLDGTDGVMLSAETAIGRFPVAAAEILQRVLTATETEYAVRVARDRLRASE
HHHHHHHHHHHCCCCCEEEEECHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
STPATNPISLVACQLAARLNAKAIIAPVRDIATALGIARFRPAAPLVVMADSEDLCRRLA
CCCCCCCHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHH
VVWGVSPLLAVAEFEPQSCLYLASHWLCAQGLAQPGDPVVMLSTSGAAQGAADTLQVMRV
HHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHEEEEEEE
DNS
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]