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The map label for this gene is nuoN [H]

Identifier: 114330938

GI number: 114330938

Start: 961973

End: 963418

Strand: Direct

Name: nuoN [H]

Synonym: Neut_0935

Alternate gene names: 114330938

Gene position: 961973-963418 (Clockwise)

Preceding gene: 114330937

Following gene: 114330940

Centisome position: 36.15

GC content: 44.12

Gene sequence:

>1446_bases
ATGGATTTTTTGTTACCTGACTTCACACCAGCTTACCCTGAAATTTTCCTGCTGTTCATGATTTGCGTGATCATGCTTGC
TGACTTGTTTGTAGGAGAAAAAAATCGGCTTCTGTCATTTTATTTGTCATTATTGACACTGGCCGGATGTTCATTCATTA
CGTATGACATATATAGCACAGAAGTTCGTTATACTTTCTCAGGCATGTTTGTCGGTGACGCAATGTCAGATACGCTAAAA
CTGATGATTTATCCTACTGTTGCGGCTGTGTTGATCTATTCCCGCGCATACATCAGTCTCCGGGGTCTATTGAAAGGGGA
GTTCTTCAGTCTCACATTGTTCGCCACACTGGGTATGATGGTTATGGTTTCTGCAAGCCACCTTATTACCCTTTATCTGG
GAATTGAACTGTTATCCCTGTCGCTTTATGCCATGGTAGCGTTGCAACGTGAATCGGCTGTTGCTACCGAAGCTGCCATC
AAATTTTTTGTTCTGGGCGCACTGGCTTCCGGGTTTTTGCTTTATGGCATGTCAATGCTATATGGCGCAACTGGAACATT
GCATATTTCGGAATTAACGAAGAGCATCCAACAAGGTACGGCAGATCATGACATCTTTATCATCGGTCTGGTTTTCGTTG
TTGCCGGTATCAGCTTTAAACTCAGCGCAGTACCATTTCATATGTGGGCTCCAGATGTTTATGAAGGAGCGCCTACTGCA
GTAACATTATTTATCGGGTCAGCACCTAAGCTGGCGGCTTTTGGTTTTGTTATGAGATTGCTGGTGGAGGGCCTGGGAGA
GCTGGCTACTGATTGGCAGGGAATGCTGGTCTTGCTGTCAGTGGCCTCGATGGCGGTTGGTAATTTTGTAGCGATAGCTC
AGCTAAATATTAAGCGGATGCTGGCTTATTCAACAATTTCGCACATAGGATTTGTTTTGCTTGGTTTTATTGCGATAGGA
GAAAACGGCTACAGCTCAGCCATGTTCTATGTGATTGCTTACGTATTAATGACGCTGGGTGCATTTGGAATTATTATGTT
GCTCTCCCGGGAAGGATTTGAGGCGGACAAGATCAGTGATTTTAAAGGTCTTAATCAACGCAGCCCGTGGCTTGCATTTA
TGATGTTGTTAATAATGTTTTCTATGGCGGGAGTTCCCCCCATGATTGGATTCTATGCAAAACTAGCTGTTTTCCAGGCT
GTCCTGGAGGCAGGGTATGTCTGGTTAGTGGTTGTTGCAGTTATACTGGCGCTAATTGGTGCCTTTTATTATTTACGTAT
CGTCAAGTTTATATATTTTGATGCACCGGAAGATACTCGACCGATTTTATTCAAACCGGATGTCAGGATACTCATCAGCA
CAAACGGATTGGCCATAGTACTTCTGGGCATGTTCCCTCAGGCACTTATGGGTTTGAGCCTGTCTGCCATTCAAAACTCC
ATGTAA

Upstream 100 bases:

>100_bases
ATAATCTGCTGGAGCATGTTGCACGTACCAAGCTTTAAGATATTTAGCTAAATTTTTGGATAATAAAAATACTTTCCTGA
TCGGAAATTAAGGATTTTTC

Downstream 100 bases:

>100_bases
AAATCATGGCAGGTGGGAGAGTTATGGCTGATTATAGCTGATACGATAAATCACACCTGCCATATCATCCGTGATCAGCA
TCGCTCCATCCGCTGTTATT

Product: NADH dehydrogenase subunit N

Products: NA

Alternate protein names: NADH dehydrogenase I subunit N; NDH-1 subunit N [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MDFLLPDFTPAYPEIFLLFMICVIMLADLFVGEKNRLLSFYLSLLTLAGCSFITYDIYSTEVRYTFSGMFVGDAMSDTLK
LMIYPTVAAVLIYSRAYISLRGLLKGEFFSLTLFATLGMMVMVSASHLITLYLGIELLSLSLYAMVALQRESAVATEAAI
KFFVLGALASGFLLYGMSMLYGATGTLHISELTKSIQQGTADHDIFIIGLVFVVAGISFKLSAVPFHMWAPDVYEGAPTA
VTLFIGSAPKLAAFGFVMRLLVEGLGELATDWQGMLVLLSVASMAVGNFVAIAQLNIKRMLAYSTISHIGFVLLGFIAIG
ENGYSSAMFYVIAYVLMTLGAFGIIMLLSREGFEADKISDFKGLNQRSPWLAFMMLLIMFSMAGVPPMIGFYAKLAVFQA
VLEAGYVWLVVVAVILALIGAFYYLRIVKFIYFDAPEDTRPILFKPDVRILISTNGLAIVLLGMFPQALMGLSLSAIQNS
M

Sequences:

>Translated_481_residues
MDFLLPDFTPAYPEIFLLFMICVIMLADLFVGEKNRLLSFYLSLLTLAGCSFITYDIYSTEVRYTFSGMFVGDAMSDTLK
LMIYPTVAAVLIYSRAYISLRGLLKGEFFSLTLFATLGMMVMVSASHLITLYLGIELLSLSLYAMVALQRESAVATEAAI
KFFVLGALASGFLLYGMSMLYGATGTLHISELTKSIQQGTADHDIFIIGLVFVVAGISFKLSAVPFHMWAPDVYEGAPTA
VTLFIGSAPKLAAFGFVMRLLVEGLGELATDWQGMLVLLSVASMAVGNFVAIAQLNIKRMLAYSTISHIGFVLLGFIAIG
ENGYSSAMFYVIAYVLMTLGAFGIIMLLSREGFEADKISDFKGLNQRSPWLAFMMLLIMFSMAGVPPMIGFYAKLAVFQA
VLEAGYVWLVVVAVILALIGAFYYLRIVKFIYFDAPEDTRPILFKPDVRILISTNGLAIVLLGMFPQALMGLSLSAIQNS
M
>Mature_481_residues
MDFLLPDFTPAYPEIFLLFMICVIMLADLFVGEKNRLLSFYLSLLTLAGCSFITYDIYSTEVRYTFSGMFVGDAMSDTLK
LMIYPTVAAVLIYSRAYISLRGLLKGEFFSLTLFATLGMMVMVSASHLITLYLGIELLSLSLYAMVALQRESAVATEAAI
KFFVLGALASGFLLYGMSMLYGATGTLHISELTKSIQQGTADHDIFIIGLVFVVAGISFKLSAVPFHMWAPDVYEGAPTA
VTLFIGSAPKLAAFGFVMRLLVEGLGELATDWQGMLVLLSVASMAVGNFVAIAQLNIKRMLAYSTISHIGFVLLGFIAIG
ENGYSSAMFYVIAYVLMTLGAFGIIMLLSREGFEADKISDFKGLNQRSPWLAFMMLLIMFSMAGVPPMIGFYAKLAVFQA
VLEAGYVWLVVVAVILALIGAFYYLRIVKFIYFDAPEDTRPILFKPDVRILISTNGLAIVLLGMFPQALMGLSLSAIQNS
M

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family [H]

Homologues:

Organism=Escherichia coli, GI145693160, Length=378, Percent_Identity=37.8306878306878, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788614, Length=409, Percent_Identity=25.9168704156479, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788827, Length=381, Percent_Identity=25.4593175853018, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1788613, Length=391, Percent_Identity=25.8312020460358, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1788831, Length=240, Percent_Identity=27.0833333333333, Blast_Score=76, Evalue=6e-15,
Organism=Escherichia coli, GI2367154, Length=259, Percent_Identity=26.2548262548263, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010096
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 52660; Mature: 52660

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLLPDFTPAYPEIFLLFMICVIMLADLFVGEKNRLLSFYLSLLTLAGCSFITYDIYST
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHEEEEEEEC
EVRYTFSGMFVGDAMSDTLKLMIYPTVAAVLIYSRAYISLRGLLKGEFFSLTLFATLGMM
EEEEEEECEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VMVSASHLITLYLGIELLSLSLYAMVALQRESAVATEAAIKFFVLGALASGFLLYGMSML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGATGTLHISELTKSIQQGTADHDIFIIGLVFVVAGISFKLSAVPFHMWAPDVYEGAPTA
HCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECEEECCCCHHHCCCCCE
VTLFIGSAPKLAAFGFVMRLLVEGLGELATDWQGMLVLLSVASMAVGNFVAIAQLNIKRM
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAYSTISHIGFVLLGFIAIGENGYSSAMFYVIAYVLMTLGAFGIIMLLSREGFEADKISD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FKGLNQRSPWLAFMMLLIMFSMAGVPPMIGFYAKLAVFQAVLEAGYVWLVVVAVILALIG
HCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AFYYLRIVKFIYFDAPEDTRPILFKPDVRILISTNGLAIVLLGMFPQALMGLSLSAIQNS
HHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHCC
M
C
>Mature Secondary Structure
MDFLLPDFTPAYPEIFLLFMICVIMLADLFVGEKNRLLSFYLSLLTLAGCSFITYDIYST
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHEEEEEEEC
EVRYTFSGMFVGDAMSDTLKLMIYPTVAAVLIYSRAYISLRGLLKGEFFSLTLFATLGMM
EEEEEEECEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
VMVSASHLITLYLGIELLSLSLYAMVALQRESAVATEAAIKFFVLGALASGFLLYGMSML
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGATGTLHISELTKSIQQGTADHDIFIIGLVFVVAGISFKLSAVPFHMWAPDVYEGAPTA
HCCCCCEEHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCEEEEECEEECCCCHHHCCCCCE
VTLFIGSAPKLAAFGFVMRLLVEGLGELATDWQGMLVLLSVASMAVGNFVAIAQLNIKRM
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LAYSTISHIGFVLLGFIAIGENGYSSAMFYVIAYVLMTLGAFGIIMLLSREGFEADKISD
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
FKGLNQRSPWLAFMMLLIMFSMAGVPPMIGFYAKLAVFQAVLEAGYVWLVVVAVILALIG
HCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
AFYYLRIVKFIYFDAPEDTRPILFKPDVRILISTNGLAIVLLGMFPQALMGLSLSAIQNS
HHHHHHHHHHHHCCCCCCCCCEEECCCEEEEEECCCHHHHHHHHHHHHHHCCHHHHHHCC
M
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]