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The map label for this gene is tpiA

Identifier: 114330923

GI number: 114330923

Start: 947356

End: 948114

Strand: Direct

Name: tpiA

Synonym: Neut_0920

Alternate gene names: 114330923

Gene position: 947356-948114 (Clockwise)

Preceding gene: 114330918

Following gene: 114330924

Centisome position: 35.6

GC content: 49.01

Gene sequence:

>759_bases
ATGACGCGTGCGGGGTTTGTGGCGGGAAACTGGAAAATGTATGGTAATTTGGTTCAGAATCAAGAATTGCTTGCCGCAAT
TGTTTTGGGTATCCCATCATTGCAGAATGCGTCCTGCGCGGTATGTGTGCCTTATCCCTACTTGGCGCAAGCGCAGTCAG
TGTTGAAGGGTACGCATGTGGCATGGGGGGGGCAGAATATCAGCCAGCATCATCAAGGTGCTTATACCGGAGAGGTATCG
GCAGGTATGCTGACCGATTTTGGTTGTCGTTATGTAATTGTGGGCCACTCGGAGCGGCGGACGCTATACGGTGAAGATAA
TCAGATGGTTGCGGAGAAATTTCACTCCGCCCAGGAGCAATCGCTAGTACCAATACTCTGTGTCGGCGAAACGCTGGCGC
AGCGTGAGGCAAATGAGACCGAACAAGTGATAGCTAGGCAGCTTGATGCGGTGATTGGTCTGGTAGGTGTTAATGCGATA
AAGCAATCAGTTATTGCATATGAGCCGGTATGGGCAATTGGTACAGGAGAAACTGCTACTCCCCAGCAGGCGCAGGATGT
TCATGCATTTGTAAGGAATCGAATAGCTGCTCATAACAGCGAAATTGCAGCAGATATCCAGATAGTTTATGGCGGAAGTG
TGAAAGCAAATAATGCGTCTGAATTATTTGCGATGCCTGACATTGACGGAGGGTTGATTGGGGGAGCTTCGTTAGTTGCT
GCAGAGTTTATTTCGATTTGTTTCGCAGCGCAAAGTTGA

Upstream 100 bases:

>100_bases
TCTTAATAATCCCCCATTTAGTTGAGAAACTCGGGCATTGACGCGATCCGGTTTCGCAGGTAGCATCCTTGGGTTTATGG
ATTTAAAGCTGAGGGACGGG

Downstream 100 bases:

>100_bases
ACGGGGATAAAGTATATTGTGGAAACACTTATTTGGTCAGTACATATCATTGCAGCAGTAGTTGTTGTTGTTTTGGTTCT
GTTGCAACAGGGGAAGGGCG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MTRAGFVAGNWKMYGNLVQNQELLAAIVLGIPSLQNASCAVCVPYPYLAQAQSVLKGTHVAWGGQNISQHHQGAYTGEVS
AGMLTDFGCRYVIVGHSERRTLYGEDNQMVAEKFHSAQEQSLVPILCVGETLAQREANETEQVIARQLDAVIGLVGVNAI
KQSVIAYEPVWAIGTGETATPQQAQDVHAFVRNRIAAHNSEIAADIQIVYGGSVKANNASELFAMPDIDGGLIGGASLVA
AEFISICFAAQS

Sequences:

>Translated_252_residues
MTRAGFVAGNWKMYGNLVQNQELLAAIVLGIPSLQNASCAVCVPYPYLAQAQSVLKGTHVAWGGQNISQHHQGAYTGEVS
AGMLTDFGCRYVIVGHSERRTLYGEDNQMVAEKFHSAQEQSLVPILCVGETLAQREANETEQVIARQLDAVIGLVGVNAI
KQSVIAYEPVWAIGTGETATPQQAQDVHAFVRNRIAAHNSEIAADIQIVYGGSVKANNASELFAMPDIDGGLIGGASLVA
AEFISICFAAQS
>Mature_251_residues
TRAGFVAGNWKMYGNLVQNQELLAAIVLGIPSLQNASCAVCVPYPYLAQAQSVLKGTHVAWGGQNISQHHQGAYTGEVSA
GMLTDFGCRYVIVGHSERRTLYGEDNQMVAEKFHSAQEQSLVPILCVGETLAQREANETEQVIARQLDAVIGLVGVNAIK
QSVIAYEPVWAIGTGETATPQQAQDVHAFVRNRIAAHNSEIAADIQIVYGGSVKANNASELFAMPDIDGGLIGGASLVAA
EFISICFAAQS

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI4507645, Length=252, Percent_Identity=41.2698412698413, Blast_Score=179, Evalue=2e-45,
Organism=Homo sapiens, GI226529917, Length=252, Percent_Identity=41.2698412698413, Blast_Score=179, Evalue=2e-45,
Organism=Escherichia coli, GI1790353, Length=250, Percent_Identity=48.4, Blast_Score=244, Evalue=3e-66,
Organism=Caenorhabditis elegans, GI17536593, Length=255, Percent_Identity=47.0588235294118, Blast_Score=197, Evalue=6e-51,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=41.5322580645161, Blast_Score=184, Evalue=1e-47,
Organism=Drosophila melanogaster, GI28572008, Length=255, Percent_Identity=45.4901960784314, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI28572006, Length=255, Percent_Identity=45.4901960784314, Blast_Score=201, Evalue=4e-52,
Organism=Drosophila melanogaster, GI28572004, Length=255, Percent_Identity=45.4901960784314, Blast_Score=201, Evalue=6e-52,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_NITEC (Q0AHK2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747145.1
- ProteinModelPortal:   Q0AHK2
- SMR:   Q0AHK2
- STRING:   Q0AHK2
- GeneID:   4272798
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0920
- NMPDR:   fig|335283.3.peg.1606
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- PhylomeDB:   Q0AHK2
- ProtClustDB:   PRK00042
- BioCyc:   NEUT335283:NEUT_0920-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26803; Mature: 26671

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 168-168 BINDING 10-10 BINDING 12-12

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRAGFVAGNWKMYGNLVQNQELLAAIVLGIPSLQNASCAVCVPYPYLAQAQSVLKGTHV
CCCCCEEECCHHEECCHHCCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHCCCEE
AWGGQNISQHHQGAYTGEVSAGMLTDFGCRYVIVGHSERRTLYGEDNQMVAEKFHSAQEQ
EECCCCHHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHC
SLVPILCVGETLAQREANETEQVIARQLDAVIGLVGVNAIKQSVIAYEPVWAIGTGETAT
CCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PQQAQDVHAFVRNRIAAHNSEIAADIQIVYGGSVKANNASELFAMPDIDGGLIGGASLVA
CHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEECCCCCCEEECCCCCCCCCCHHHHHH
AEFISICFAAQS
HHHHHHHHHCCC
>Mature Secondary Structure 
TRAGFVAGNWKMYGNLVQNQELLAAIVLGIPSLQNASCAVCVPYPYLAQAQSVLKGTHV
CCCCEEECCHHEECCHHCCHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHCCCEE
AWGGQNISQHHQGAYTGEVSAGMLTDFGCRYVIVGHSERRTLYGEDNQMVAEKFHSAQEQ
EECCCCHHHHCCCCCCCCCCCCHHCCCCCEEEEEECCCCCEEECCCCHHHHHHHHHHHHC
SLVPILCVGETLAQREANETEQVIARQLDAVIGLVGVNAIKQSVIAYEPVWAIGTGETAT
CCCEEEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCC
PQQAQDVHAFVRNRIAAHNSEIAADIQIVYGGSVKANNASELFAMPDIDGGLIGGASLVA
CHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEECCCCCCEEECCCCCCCCCCHHHHHH
AEFISICFAAQS
HHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA