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The map label for this gene is mlaE [H]

Identifier: 114330858

GI number: 114330858

Start: 870346

End: 871143

Strand: Direct

Name: mlaE [H]

Synonym: Neut_0852

Alternate gene names: 114330858

Gene position: 870346-871143 (Clockwise)

Preceding gene: 114330857

Following gene: 114330859

Centisome position: 32.71

GC content: 51.0

Gene sequence:

>798_bases
TTGGCTAGACGGATCAGGATCGTTATCGAATCCATTGGGCATAGGATGGTTGATAGCATCTGGCGACTTGGATGTGCAAC
TCATTTTATGCTGCTGGTATTGCTGAAATCTGTTCCCGGTTTTCTGCGTTTTCGTTTGATTACGCGCGAGATCTATTATG
CCGGAGTGATGTCGCTGATTATTATTCTGGTATCCGGGCTATTTGTCGGCATGGTGCTGGGATTGCAGGGTTATGAAACG
CTGCAGAAATTTGGTTCCGAATCAGCGGTGGGAACATTGGTGGCGTTATCTCTGGTGCGCGAACTGGGGCCAGTGATTGC
TGCATTGCTGTTTGCCAGCCGGGCAGGTTCAGCCATGACTGCCGAAATCGGACTGATGAAAGCAACCGAGCAACTGGCCG
CCATGGGTATGATGGCAGTGGACCCGATCGCGCGCATCGTTGCGCCGCGATTCTGGGGTGGAGTTTTTTCGATGCCGTTG
CTGGCTGCCATGTTTTCTGTGATGGGTGTGTTTGGTGGCTATCTGGTGACGGTGATTTTTATCGGTGTGGACGAGGGCTC
TTTTTGGTCACAGATGCAAAGCTCGGTTGATTTCCGTTATGACATTGTAAATGGTGTCATCAAAAGCTGTTTCTTTGGTG
TGGTGGTGACGGCCATTGCCGTGTTTGAAGGATTTGATGCACCGCCAACGGCAGAGGGGGTGTCGGGCGCAACGACCCGT
ACAGTGGTTACTTCCGCGCTGGCAATTTTGGGTCTGGATTTTGTTTTAACTGCATTTATGTTCAGAGGGGTAAGCTGA

Upstream 100 bases:

>100_bases
TTTCATTACCAGGCACAGGCTTATTCTCAGGATTTGGGAATAGGCGTTGGAAGCAATGCCTGACTGGATATTATCAGCAT
GAATACAGGGAGTGATCAGT

Downstream 100 bases:

>100_bases
TGCAACGTACCATGATGGATTTTTGGGTGGGCCTGTTCGTCATGGCGGGGATTGGTGCATTGCTGATTCTGGGGTTAAAA
GTAGGTAACCTTACGGATTT

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MARRIRIVIESIGHRMVDSIWRLGCATHFMLLVLLKSVPGFLRFRLITREIYYAGVMSLIIILVSGLFVGMVLGLQGYET
LQKFGSESAVGTLVALSLVRELGPVIAALLFASRAGSAMTAEIGLMKATEQLAAMGMMAVDPIARIVAPRFWGGVFSMPL
LAAMFSVMGVFGGYLVTVIFIGVDEGSFWSQMQSSVDFRYDIVNGVIKSCFFGVVVTAIAVFEGFDAPPTAEGVSGATTR
TVVTSALAILGLDFVLTAFMFRGVS

Sequences:

>Translated_265_residues
MARRIRIVIESIGHRMVDSIWRLGCATHFMLLVLLKSVPGFLRFRLITREIYYAGVMSLIIILVSGLFVGMVLGLQGYET
LQKFGSESAVGTLVALSLVRELGPVIAALLFASRAGSAMTAEIGLMKATEQLAAMGMMAVDPIARIVAPRFWGGVFSMPL
LAAMFSVMGVFGGYLVTVIFIGVDEGSFWSQMQSSVDFRYDIVNGVIKSCFFGVVVTAIAVFEGFDAPPTAEGVSGATTR
TVVTSALAILGLDFVLTAFMFRGVS
>Mature_264_residues
ARRIRIVIESIGHRMVDSIWRLGCATHFMLLVLLKSVPGFLRFRLITREIYYAGVMSLIIILVSGLFVGMVLGLQGYETL
QKFGSESAVGTLVALSLVRELGPVIAALLFASRAGSAMTAEIGLMKATEQLAAMGMMAVDPIARIVAPRFWGGVFSMPLL
AAMFSVMGVFGGYLVTVIFIGVDEGSFWSQMQSSVDFRYDIVNGVIKSCFFGVVVTAIAVFEGFDAPPTAEGVSGATTRT
VVTSALAILGLDFVLTAFMFRGVS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=254, Percent_Identity=56.6929133858268, Blast_Score=283, Evalue=9e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 28449; Mature: 28318

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ARRIRIVIESIGHRMVDSIWRLGCATHFMLLVLLKSVPGFLRFRLITREIYYAGVMSLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHH
IILVSGLFVGMVLGLQGYETLQKFGSESAVGTLVALSLVRELGPVIAALLFASRAGSAMT
HHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
AEIGLMKATEQLAAMGMMAVDPIARIVAPRFWGGVFSMPLLAAMFSVMGVFGGYLVTVIF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IGVDEGSFWSQMQSSVDFRYDIVNGVIKSCFFGVVVTAIAVFEGFDAPPTAEGVSGATTR
HCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHH
TVVTSALAILGLDFVLTAFMFRGVS
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]