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The map label for this gene is ttcA

Identifier: 114330769

GI number: 114330769

Start: 774207

End: 775148

Strand: Direct

Name: ttcA

Synonym: Neut_0756

Alternate gene names: 114330769

Gene position: 774207-775148 (Clockwise)

Preceding gene: 114330766

Following gene: 114330775

Centisome position: 29.09

GC content: 46.82

Gene sequence:

>942_bases
ATGATTACGGATACATTTTCCAGAAAATCCGAATACAGCACTAATAAGTTGCGTAAAAGACTGAGGCGGCTTGTTGGCAC
GGCTATTGCCGATTTTAATATGATTGAGAGCGGTGACCGGATTATGGTGTGTTTGTCCGGTGGAAAGGATAGCTATGCGC
TGCTGGATATTTTGCGCAATTTGCAAGCACATGCGCCACTGGATTTCGAACTGATTGCAGTTAATCTGGACCAGAAGCAG
CCGGGTTTTCCTGAGCATGTATTGCCTGGTTATTTGTCAGAAATAAATATGCCCTTTCGTATTGTTGAACAGGATACCTA
TAGTGTTGTAAAACGAGTGATTCCTGAGGGGAAAACGACCTGTAGCCTGTGTTCCCGTTTGCGTAGAGGAGTGCTTTACC
GGGTGGCAACCGAGCTGGGGGCAACCAAGATTGCCCTGGGACACCATCGCGACGATATTCTGGAGACTTTTTTCCTGAAT
ATGTTTTATGGGGGCAAACTGAAAGCAATGCCTCCCAAACTGGTAAGTGATGACGGGTGTCATGTGGTAATTCGACCGCT
GGCATATTGCAAGGAAAAAGATCTGGCTGCTTATGCCCAGCTTGTCCAGTTTCCTATTATTCCTTGCAATTTATGTGGTT
CGCAGCCGAACCTGCAGCGGCAGGTAATTAAGGAAATGATGCAGCAATGGAACAAGAAATACCCCGGTCGGCTTGAAACG
ATGTTTACAGCTTTACAAAATATTCAGCTTTCACACTTGGCGGATACCAGTCGCTATGATTTTGTCGGAATCAAACCACA
TGGTATTGCGACGGAAGAAGGGGACAAGGCATTTGATGAGGAGTTACTTCCTGACAGCCCGGTTGGTATGAATCATGATG
ATGCCGCATCCGAGCTGGATGAAGCATGCAGTACCGAGGCTGTCCAGGGTGGCACTATATAG

Upstream 100 bases:

>100_bases
GTGTTAATTTATACAAATGTTCTTTCCTTAACTTTGTGTGAAATAGTTACCTGGCTGATTTTTTTGATAGCTTTCTTATT
TTATTTTTCTATAAATTCAT

Downstream 100 bases:

>100_bases
CATGTCTTCTTCAGTATATCTCTGCTTTATTCAATCCCGGCTTCGTGCGCTTGTTGATCAGCATGATAGCTGGAGCGTAC
CATTGGACCGCAAGCAGCGT

Product: C32 tRNA thiolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MITDTFSRKSEYSTNKLRKRLRRLVGTAIADFNMIESGDRIMVCLSGGKDSYALLDILRNLQAHAPLDFELIAVNLDQKQ
PGFPEHVLPGYLSEINMPFRIVEQDTYSVVKRVIPEGKTTCSLCSRLRRGVLYRVATELGATKIALGHHRDDILETFFLN
MFYGGKLKAMPPKLVSDDGCHVVIRPLAYCKEKDLAAYAQLVQFPIIPCNLCGSQPNLQRQVIKEMMQQWNKKYPGRLET
MFTALQNIQLSHLADTSRYDFVGIKPHGIATEEGDKAFDEELLPDSPVGMNHDDAASELDEACSTEAVQGGTI

Sequences:

>Translated_313_residues
MITDTFSRKSEYSTNKLRKRLRRLVGTAIADFNMIESGDRIMVCLSGGKDSYALLDILRNLQAHAPLDFELIAVNLDQKQ
PGFPEHVLPGYLSEINMPFRIVEQDTYSVVKRVIPEGKTTCSLCSRLRRGVLYRVATELGATKIALGHHRDDILETFFLN
MFYGGKLKAMPPKLVSDDGCHVVIRPLAYCKEKDLAAYAQLVQFPIIPCNLCGSQPNLQRQVIKEMMQQWNKKYPGRLET
MFTALQNIQLSHLADTSRYDFVGIKPHGIATEEGDKAFDEELLPDSPVGMNHDDAASELDEACSTEAVQGGTI
>Mature_313_residues
MITDTFSRKSEYSTNKLRKRLRRLVGTAIADFNMIESGDRIMVCLSGGKDSYALLDILRNLQAHAPLDFELIAVNLDQKQ
PGFPEHVLPGYLSEINMPFRIVEQDTYSVVKRVIPEGKTTCSLCSRLRRGVLYRVATELGATKIALGHHRDDILETFFLN
MFYGGKLKAMPPKLVSDDGCHVVIRPLAYCKEKDLAAYAQLVQFPIIPCNLCGSQPNLQRQVIKEMMQQWNKKYPGRLET
MFTALQNIQLSHLADTSRYDFVGIKPHGIATEEGDKAFDEELLPDSPVGMNHDDAASELDEACSTEAVQGGTI

Specific function: Required for the thiolation of cytidine in position 32 of tRNA, to form 2-thiocytidine (s(2)C32)

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TtcA family

Homologues:

Organism=Escherichia coli, GI1787606, Length=296, Percent_Identity=67.2297297297297, Blast_Score=434, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI193211090, Length=239, Percent_Identity=33.0543933054393, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17540090, Length=222, Percent_Identity=27.027027027027, Blast_Score=85, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24651972, Length=220, Percent_Identity=28.1818181818182, Blast_Score=79, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TTCA_NITEC (Q0AI06)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746991.1
- ProteinModelPortal:   Q0AI06
- SMR:   Q0AI06
- STRING:   Q0AI06
- GeneID:   4273015
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0756
- NMPDR:   fig|335283.3.peg.2397
- eggNOG:   COG0037
- HOGENOM:   HBG347038
- OMA:   FPHAREE
- PhylomeDB:   Q0AI06
- ProtClustDB:   PRK10696
- BioCyc:   NEUT335283:NEUT_0756-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01850
- InterPro:   IPR012089
- InterPro:   IPR014729
- InterPro:   IPR011063
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF004976

Pfam domain/function: PF01171 ATP_bind_3

EC number: NA

Molecular weight: Translated: 35070; Mature: 35070

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITDTFSRKSEYSTNKLRKRLRRLVGTAIADFNMIESGDRIMVCLSGGKDSYALLDILRN
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
LQAHAPLDFELIAVNLDQKQPGFPEHVLPGYLSEINMPFRIVEQDTYSVVKRVIPEGKTT
HHHCCCCCEEEEEEEECCCCCCCCCHHCCHHHHHCCCCEEEECHHHHHHHHHHCCCCCHH
CSLCSRLRRGVLYRVATELGATKIALGHHRDDILETFFLNMFYGGKLKAMPPKLVSDDGC
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCC
HVVIRPLAYCKEKDLAAYAQLVQFPIIPCNLCGSQPNLQRQVIKEMMQQWNKKYPGRLET
EEEEHHHHHHCCHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
MFTALQNIQLSHLADTSRYDFVGIKPHGIATEEGDKAFDEELLPDSPVGMNHDDAASELD
HHHHHHHCHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHH
EACSTEAVQGGTI
HHHHHHCCCCCCC
>Mature Secondary Structure
MITDTFSRKSEYSTNKLRKRLRRLVGTAIADFNMIESGDRIMVCLSGGKDSYALLDILRN
CCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHH
LQAHAPLDFELIAVNLDQKQPGFPEHVLPGYLSEINMPFRIVEQDTYSVVKRVIPEGKTT
HHHCCCCCEEEEEEEECCCCCCCCCHHCCHHHHHCCCCEEEECHHHHHHHHHHCCCCCHH
CSLCSRLRRGVLYRVATELGATKIALGHHRDDILETFFLNMFYGGKLKAMPPKLVSDDGC
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHCCCEECCCCCCCCCCCCC
HVVIRPLAYCKEKDLAAYAQLVQFPIIPCNLCGSQPNLQRQVIKEMMQQWNKKYPGRLET
EEEEHHHHHHCCHHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCCCHHHH
MFTALQNIQLSHLADTSRYDFVGIKPHGIATEEGDKAFDEELLPDSPVGMNHDDAASELD
HHHHHHHCHHHHHCCCCCCCEEECCCCCCCCCCCCHHHHHHCCCCCCCCCCCCHHHHHHH
EACSTEAVQGGTI
HHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA