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The map label for this gene is fliF [H]

Identifier: 114330758

GI number: 114330758

Start: 764446

End: 766089

Strand: Reverse

Name: fliF [H]

Synonym: Neut_0745

Alternate gene names: 114330758

Gene position: 766089-764446 (Counterclockwise)

Preceding gene: 114330763

Following gene: 114330757

Centisome position: 28.79

GC content: 51.28

Gene sequence:

>1644_bases
ATGGCAACTATTCAAGAAGAAATCCAGGGAGAGAAAAAGGCATTTAGTCAGCTGGATAAAATCAAACAACTGCCGGCCCA
AAAGAAGCTGGGATTGATGGTGGCAGCAGCTGCCGTGATCGCACTATTCGCAGGAACATGGCTGTGGAGTCAGACACCGG
ATTACCGCGTGCTTTACGCAAACTTATCGGAGCAGGAAGGCGGTGCAATCATCGATGCCCTGCAAAAGATGAACGTTCCA
TACCAGTTCAGCGAGAGTAGTGGCTCGATTCTGGTTCCGGTCAATCAGGTGCATGAAGTCCGTCTGCATCTGGCAGGACA
GGGATTACCCAAGGGCAATCTATCTGGTTTCGAGATATTGGAAAACCAGAAATTTGGCTCTAGTCAGTTTCTGGAACAAG
TCAATTACCAGCGCGCACTGGAAGGCGAGCTAGCCCGCTCGATCCAGTCCTTGTCAGCCGTGCAGGGAGCACGGGTGCAC
CTGGCCATTGCCAGGCCAAGCGTGTTTACCCGGGAACGCCAACAACCAAGTGTTTCTGTACTGCTGAACCTGCACCATGG
ACGCATGCTGAGCCCCGAACAGGTCAGTGCCATCGTCCATCTGGTTTCCAGCAGCATTCCCGATCTGACGGTCAAAAATG
TCACAGTTGTTGATCAGCAGGGCAATTTGTTAAGCGGCCAGAAAGAAAATCAGATGGAAACCGGGCTTGACCCCGGCCAG
CTTAAATATGTACAGGATATGGAACAGAATTTCGTCCGGCGCATTGAAGCTATTCTGACGCCTGTCACCGGCCAGGATAA
TGTACATGCACAGGTGACTGCGGATATCGATTTTTCCCGCATTGAGCGTTCCGAAGAAACTTACCGACCCAACAACAACC
CAGAAGAATCTGCAGCCGTTCGTAGCCAGCAGAAATCCGAATCGGTTTCTATTTCACAACCCGATGGCGGCGTGCCTGGT
GCGCTGTCAAATCGCCCTCCTGCCCCGGCCCAGGCACCGGTAGAAACCAAAGAAGGTCAGGAGGAAACTGCTCAGACAAC
GCCTACCGATACCCGGAAGGAATCCACCACTAACTACGAAGTGGATAAAACCATTTCACATATCCGCCAGTCAACCGGGC
AGATCAACCGACTTTCAGCAGCCGTGGTAGTCAATTACCGCACAAGGATGAATGAAGAAGGCAACCCGATCAACGAACCG
CTTTCTGGCGAAGAAATTGAAAAGATTACGGCGTTGGTCAAGCAGGCCATCGGTTTCAGCGAGGCTCGCGGCGACACATT
GACCGTCACCAACAGTCAATTCAGGTTGAAAGAGGAAATTCTGGAAGAACTGCCGCTCTGGAAAGATCCGGATACTGTTC
TGCTCGCCAAAGATATTGGCAAACAGTTGCTAATTGCCGCCATTGTGCTGTTCTTCCTGCAAAAAATCTTCCGGCCATTT
CTGAAAAATCTGTTGCCTCCTCCACCGCCTGCTCCTGTTCCGGCATTAACTGCCAAGGATTCAGAACAGGATGAAGCTGC
GATCAATACGATCAAGGTAATGACTCTGGAACAAAATCTGGAAAAGGCCAGATTACTGGCTGCCAATGAACCGGCCATTG
TGGCCAACGTCGTCAAGGGATGGGTATCCGGCAATGAACGATGA

Upstream 100 bases:

>100_bases
ATTCGTTTAACCACGTTCGATATTCATATGCCAAACCAGAATCAGCTCAAATATTCGTTAAGCTGTGTCAATCCCGATCA
ACCGGGCAGGAGGTGATTTC

Downstream 100 bases:

>100_bases
AGGAATATTAAAAAGTGCAATCCTGTTGATGTCGCTGGGAGAAGAAGAAGCTTCCCGGGTTTTTCGCTATCTTGGCCCCA
AGGAAGTACAGAAACTGAGT

Product: flagellar M-ring protein FliF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 547; Mature: 546

Protein sequence:

>547_residues
MATIQEEIQGEKKAFSQLDKIKQLPAQKKLGLMVAAAAVIALFAGTWLWSQTPDYRVLYANLSEQEGGAIIDALQKMNVP
YQFSESSGSILVPVNQVHEVRLHLAGQGLPKGNLSGFEILENQKFGSSQFLEQVNYQRALEGELARSIQSLSAVQGARVH
LAIARPSVFTRERQQPSVSVLLNLHHGRMLSPEQVSAIVHLVSSSIPDLTVKNVTVVDQQGNLLSGQKENQMETGLDPGQ
LKYVQDMEQNFVRRIEAILTPVTGQDNVHAQVTADIDFSRIERSEETYRPNNNPEESAAVRSQQKSESVSISQPDGGVPG
ALSNRPPAPAQAPVETKEGQEETAQTTPTDTRKESTTNYEVDKTISHIRQSTGQINRLSAAVVVNYRTRMNEEGNPINEP
LSGEEIEKITALVKQAIGFSEARGDTLTVTNSQFRLKEEILEELPLWKDPDTVLLAKDIGKQLLIAAIVLFFLQKIFRPF
LKNLLPPPPPAPVPALTAKDSEQDEAAINTIKVMTLEQNLEKARLLAANEPAIVANVVKGWVSGNER

Sequences:

>Translated_547_residues
MATIQEEIQGEKKAFSQLDKIKQLPAQKKLGLMVAAAAVIALFAGTWLWSQTPDYRVLYANLSEQEGGAIIDALQKMNVP
YQFSESSGSILVPVNQVHEVRLHLAGQGLPKGNLSGFEILENQKFGSSQFLEQVNYQRALEGELARSIQSLSAVQGARVH
LAIARPSVFTRERQQPSVSVLLNLHHGRMLSPEQVSAIVHLVSSSIPDLTVKNVTVVDQQGNLLSGQKENQMETGLDPGQ
LKYVQDMEQNFVRRIEAILTPVTGQDNVHAQVTADIDFSRIERSEETYRPNNNPEESAAVRSQQKSESVSISQPDGGVPG
ALSNRPPAPAQAPVETKEGQEETAQTTPTDTRKESTTNYEVDKTISHIRQSTGQINRLSAAVVVNYRTRMNEEGNPINEP
LSGEEIEKITALVKQAIGFSEARGDTLTVTNSQFRLKEEILEELPLWKDPDTVLLAKDIGKQLLIAAIVLFFLQKIFRPF
LKNLLPPPPPAPVPALTAKDSEQDEAAINTIKVMTLEQNLEKARLLAANEPAIVANVVKGWVSGNER
>Mature_546_residues
ATIQEEIQGEKKAFSQLDKIKQLPAQKKLGLMVAAAAVIALFAGTWLWSQTPDYRVLYANLSEQEGGAIIDALQKMNVPY
QFSESSGSILVPVNQVHEVRLHLAGQGLPKGNLSGFEILENQKFGSSQFLEQVNYQRALEGELARSIQSLSAVQGARVHL
AIARPSVFTRERQQPSVSVLLNLHHGRMLSPEQVSAIVHLVSSSIPDLTVKNVTVVDQQGNLLSGQKENQMETGLDPGQL
KYVQDMEQNFVRRIEAILTPVTGQDNVHAQVTADIDFSRIERSEETYRPNNNPEESAAVRSQQKSESVSISQPDGGVPGA
LSNRPPAPAQAPVETKEGQEETAQTTPTDTRKESTTNYEVDKTISHIRQSTGQINRLSAAVVVNYRTRMNEEGNPINEPL
SGEEIEKITALVKQAIGFSEARGDTLTVTNSQFRLKEEILEELPLWKDPDTVLLAKDIGKQLLIAAIVLFFLQKIFRPFL
KNLLPPPPPAPVPALTAKDSEQDEAAINTIKVMTLEQNLEKARLLAANEPAIVANVVKGWVSGNER

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=551, Percent_Identity=45.0090744101633, Blast_Score=428, Evalue=1e-121,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60142; Mature: 60011

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIQEEIQGEKKAFSQLDKIKQLPAQKKLGLMVAAAAVIALFAGTWLWSQTPDYRVLYA
CCCCHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NLSEQEGGAIIDALQKMNVPYQFSESSGSILVPVNQVHEVRLHLAGQGLPKGNLSGFEIL
ECCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHH
ENQKFGSSQFLEQVNYQRALEGELARSIQSLSAVQGARVHLAIARPSVFTRERQQPSVSV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCEEE
LLNLHHGRMLSPEQVSAIVHLVSSSIPDLTVKNVTVVDQQGNLLSGQKENQMETGLDPGQ
EEEECCCCCCCHHHHHHHHHHHHCCCCCCEECEEEEEECCCCCCCCCCCCHHHCCCCCHH
LKYVQDMEQNFVRRIEAILTPVTGQDNVHAQVTADIDFSRIERSEETYRPNNNPEESAAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCHHHCCCCCCCHHHHHH
RSQQKSESVSISQPDGGVPGALSNRPPAPAQAPVETKEGQEETAQTTPTDTRKESTTNYE
HHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCH
VDKTISHIRQSTGQINRLSAAVVVNYRTRMNEEGNPINEPLSGEEIEKITALVKQAIGFS
HHHHHHHHHHHCCCHHHHEEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EARGDTLTVTNSQFRLKEEILEELPLWKDPDTVLLAKDIGKQLLIAAIVLFFLQKIFRPF
HCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LKNLLPPPPPAPVPALTAKDSEQDEAAINTIKVMTLEQNLEKARLLAANEPAIVANVVKG
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEHHHHHHHHHHCCCCCHHHHHHHHH
WVSGNER
HHCCCCC
>Mature Secondary Structure 
ATIQEEIQGEKKAFSQLDKIKQLPAQKKLGLMVAAAAVIALFAGTWLWSQTPDYRVLYA
CCCHHHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEE
NLSEQEGGAIIDALQKMNVPYQFSESSGSILVPVNQVHEVRLHLAGQGLPKGNLSGFEIL
ECCCCCCCHHHHHHHHCCCCEEECCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHH
ENQKFGSSQFLEQVNYQRALEGELARSIQSLSAVQGARVHLAIARPSVFTRERQQPSVSV
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHCCCCCEEE
LLNLHHGRMLSPEQVSAIVHLVSSSIPDLTVKNVTVVDQQGNLLSGQKENQMETGLDPGQ
EEEECCCCCCCHHHHHHHHHHHHCCCCCCEECEEEEEECCCCCCCCCCCCHHHCCCCCHH
LKYVQDMEQNFVRRIEAILTPVTGQDNVHAQVTADIDFSRIERSEETYRPNNNPEESAAV
HHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHCCHHHCCCCCCCHHHHHH
RSQQKSESVSISQPDGGVPGALSNRPPAPAQAPVETKEGQEETAQTTPTDTRKESTTNYE
HHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCH
VDKTISHIRQSTGQINRLSAAVVVNYRTRMNEEGNPINEPLSGEEIEKITALVKQAIGFS
HHHHHHHHHHHCCCHHHHEEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCH
EARGDTLTVTNSQFRLKEEILEELPLWKDPDTVLLAKDIGKQLLIAAIVLFFLQKIFRPF
HCCCCEEEEECCHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHH
LKNLLPPPPPAPVPALTAKDSEQDEAAINTIKVMTLEQNLEKARLLAANEPAIVANVVKG
HHHCCCCCCCCCCCCCCCCCCCHHHHHHHHEEEEEEHHHHHHHHHHCCCCCHHHHHHHHH
WVSGNER
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 1551848; 8224881 [H]