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The map label for this gene is glgB [H]

Identifier: 114330729

GI number: 114330729

Start: 720323

End: 722200

Strand: Direct

Name: glgB [H]

Synonym: Neut_0714

Alternate gene names: 114330729

Gene position: 720323-722200 (Clockwise)

Preceding gene: 114330724

Following gene: 114330730

Centisome position: 27.07

GC content: 51.7

Gene sequence:

>1878_bases
ATGGCTAGCAACTTGCTTCCTCTCAGCATTCTGGGTGCCGTCGAGACCAATGGCACGGTGAGTTTCGGATTCTGGCTACC
TTGGGTGTCGGCTGTCGACGGTAATGCAGTCACGGTCAAGATTATTCACGAGCACGATCAGTTTTTGCAGGAGATTCCGC
CACGGGAATTCCCCATGGCCCATAGCGTGCGCTCGCCTTACGGCGACTTTTGGTCGGCGACTGTGCCGATTGTCGGGACG
ACACCGGCAAATCCCGGATCGGCCTGGGGTACGGCTGGACGGTATGTCTATCGCTATCAGATCAACAATCCGAATGTCGG
CATCCTCGACTGGATCATCGATCCGTGCGCGCGGGAGTTTGGCGTTGGTAAGCTTTCAGCGTTTACGCTCGGTTATCAAC
CGTATAACTGGAGTGTAGCCGAGGCAAATTGGCGCACGCCGGAGCTCTCTAATCTGATAATTTATGAAATTAACATCGCG
GAGCTTGGCGGCGACCTGGAGCGCACGCGCGATCTCATGGTCTATCTTAGCGATCTCGGCGTGAACGCTATCGAAATCAT
GCCGTTGTCAAACGTTGCCATGTCGGTCGATTGGGGATATCTCCCCATCGGCTATTTCGGCGTTGATGAACGATTCGGTA
AGCGCTCTGACTTCCAGAAACTCGTTGATATTGCTCATCAGCAAGGCATCGCCGTCATTGTCGACGTCGTATATGGTCAT
ACCGGAGTCGATTTTCCCTACTACGACGCTTACAATCGCCTGCAGTATCACGAAAACCCTTTTATGGGACCTTTCGCCAA
AGATTATTTCAGCAGCTTTGGAAAGAGCACGAACTTTGGGAGCCCATTCACACGCGACTATTTTTTCACGGTTAACCATC
ATTGGCTCGAGGTTTATCACATCGACGGGCTGCGCTACGATTGCGTGCCGAATTACTGGGATGGGCCATTCGGTGTGGGA
TATGCAAACCTTGTCTATGAGACTTACCAACTCACCAAAAACAAGATTGCGCAAAATCAGCGCTATTGGAATCGTTTCAA
CGCGGGTGCAGGCACGTCGCTCAGGCTCATTCAATATGCCGAGCAATTAGAAGGGCCAGAGGAGGTGCTGCGCACAACTT
ACTCCAACAGCACCTGGCAGAATCATACCTTCGACGCTGCCAAGGCGGTGGCACGTGGCGACCAGAGGCGACTGGCTGAC
TGGGGTTTATCGCTCGGTTTACTTGGTTACCCGGAGCAAGAGAACACCAATGGAGACGTGATTACTAAAACTGCGTTGCA
ATACATTGAGAATCACGATCACGAGCGCTTCATTTGTAATTTCGGTTTGATGAATCCGGACGAGGCCGGTAATCCGCTTT
TTCTCAAGGGCGACCGCAGTCACTGGTACATGCTCCAGCCCTATCTCATCGCCATGCTGATGAGTAAAGGCGTACCGATG
TTGTGGCAAGGTGAGGAATTTGGTGAAAGCTATTTTTTGCCCGATTTCGGCGCAGGGCGTGTGGGGCTTTTGCGCTCGCT
GCGGTGGGATTATTTCTACGACAGCCCGGGTCAGCAGTTGGTGCAACTGGTAAGAAAGCTGTTACGTATCCGGCGCGATC
GGTCGCAGATCCGTCGCGGCAATTATTTTTTCTTTAATGATTGGGACCGCTATCTTTCAAAGCGTGTCTTGCTGTTCGCA
CGTTACAGCAACGCACAATACACACTTGTTGCGATTAACATCGGCGATATTGATCAGTCGGTACCTTTCTGGTTTCCCAT
CGGCGGTGACTACAATGAAGAACTTCATGGCGGTGATCTGGGGCTCAAAGGAATTACACCTCTTCAACAAACTTGGCTCA
AGGTTCCTTACCATTATGGTCGGATTTGGACGGTGTAG

Upstream 100 bases:

>100_bases
CCGGCACATTCAATGGAAGGCGTTGTGAGCAGCTTGTCGGATAAACAATTGATAATTGCGTGGTGGTTCGATTGATTGAT
CTTTATGGAGATTTGCTCTT

Downstream 100 bases:

>100_bases
GAGTCGCAAGGGTATTTATTGTTGGATAAATAAAAATTTGATAAGAGGGTTCCATGTTCCCGCTACTCTTGATTTTGATG
GTCGCCAGCATACCTAAATG

Product: alpha amylase, catalytic region

Products: NA

Alternate protein names: 1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase; Glycogen-branching enzyme; BE [H]

Number of amino acids: Translated: 625; Mature: 624

Protein sequence:

>625_residues
MASNLLPLSILGAVETNGTVSFGFWLPWVSAVDGNAVTVKIIHEHDQFLQEIPPREFPMAHSVRSPYGDFWSATVPIVGT
TPANPGSAWGTAGRYVYRYQINNPNVGILDWIIDPCAREFGVGKLSAFTLGYQPYNWSVAEANWRTPELSNLIIYEINIA
ELGGDLERTRDLMVYLSDLGVNAIEIMPLSNVAMSVDWGYLPIGYFGVDERFGKRSDFQKLVDIAHQQGIAVIVDVVYGH
TGVDFPYYDAYNRLQYHENPFMGPFAKDYFSSFGKSTNFGSPFTRDYFFTVNHHWLEVYHIDGLRYDCVPNYWDGPFGVG
YANLVYETYQLTKNKIAQNQRYWNRFNAGAGTSLRLIQYAEQLEGPEEVLRTTYSNSTWQNHTFDAAKAVARGDQRRLAD
WGLSLGLLGYPEQENTNGDVITKTALQYIENHDHERFICNFGLMNPDEAGNPLFLKGDRSHWYMLQPYLIAMLMSKGVPM
LWQGEEFGESYFLPDFGAGRVGLLRSLRWDYFYDSPGQQLVQLVRKLLRIRRDRSQIRRGNYFFFNDWDRYLSKRVLLFA
RYSNAQYTLVAINIGDIDQSVPFWFPIGGDYNEELHGGDLGLKGITPLQQTWLKVPYHYGRIWTV

Sequences:

>Translated_625_residues
MASNLLPLSILGAVETNGTVSFGFWLPWVSAVDGNAVTVKIIHEHDQFLQEIPPREFPMAHSVRSPYGDFWSATVPIVGT
TPANPGSAWGTAGRYVYRYQINNPNVGILDWIIDPCAREFGVGKLSAFTLGYQPYNWSVAEANWRTPELSNLIIYEINIA
ELGGDLERTRDLMVYLSDLGVNAIEIMPLSNVAMSVDWGYLPIGYFGVDERFGKRSDFQKLVDIAHQQGIAVIVDVVYGH
TGVDFPYYDAYNRLQYHENPFMGPFAKDYFSSFGKSTNFGSPFTRDYFFTVNHHWLEVYHIDGLRYDCVPNYWDGPFGVG
YANLVYETYQLTKNKIAQNQRYWNRFNAGAGTSLRLIQYAEQLEGPEEVLRTTYSNSTWQNHTFDAAKAVARGDQRRLAD
WGLSLGLLGYPEQENTNGDVITKTALQYIENHDHERFICNFGLMNPDEAGNPLFLKGDRSHWYMLQPYLIAMLMSKGVPM
LWQGEEFGESYFLPDFGAGRVGLLRSLRWDYFYDSPGQQLVQLVRKLLRIRRDRSQIRRGNYFFFNDWDRYLSKRVLLFA
RYSNAQYTLVAINIGDIDQSVPFWFPIGGDYNEELHGGDLGLKGITPLQQTWLKVPYHYGRIWTV
>Mature_624_residues
ASNLLPLSILGAVETNGTVSFGFWLPWVSAVDGNAVTVKIIHEHDQFLQEIPPREFPMAHSVRSPYGDFWSATVPIVGTT
PANPGSAWGTAGRYVYRYQINNPNVGILDWIIDPCAREFGVGKLSAFTLGYQPYNWSVAEANWRTPELSNLIIYEINIAE
LGGDLERTRDLMVYLSDLGVNAIEIMPLSNVAMSVDWGYLPIGYFGVDERFGKRSDFQKLVDIAHQQGIAVIVDVVYGHT
GVDFPYYDAYNRLQYHENPFMGPFAKDYFSSFGKSTNFGSPFTRDYFFTVNHHWLEVYHIDGLRYDCVPNYWDGPFGVGY
ANLVYETYQLTKNKIAQNQRYWNRFNAGAGTSLRLIQYAEQLEGPEEVLRTTYSNSTWQNHTFDAAKAVARGDQRRLADW
GLSLGLLGYPEQENTNGDVITKTALQYIENHDHERFICNFGLMNPDEAGNPLFLKGDRSHWYMLQPYLIAMLMSKGVPML
WQGEEFGESYFLPDFGAGRVGLLRSLRWDYFYDSPGQQLVQLVRKLLRIRRDRSQIRRGNYFFFNDWDRYLSKRVLLFAR
YSNAQYTLVAINIGDIDQSVPFWFPIGGDYNEELHGGDLGLKGITPLQQTWLKVPYHYGRIWTV

Specific function: Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position [H]

COG id: COG0296

COG function: function code G; 1,4-alpha-glucan branching enzyme

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 13 family [H]

Homologues:

Organism=Escherichia coli, GI1789839, Length=258, Percent_Identity=25.1937984496124, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006407
- InterPro:   IPR006048
- InterPro:   IPR013780
- InterPro:   IPR006047
- InterPro:   IPR004193
- InterPro:   IPR017853
- InterPro:   IPR013781
- InterPro:   IPR013783
- InterPro:   IPR014756 [H]

Pfam domain/function: PF00128 Alpha-amylase; PF02806 Alpha-amylase_C; PF02922 CBM_48 [H]

EC number: =2.4.1.18 [H]

Molecular weight: Translated: 71654; Mature: 71522

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASNLLPLSILGAVETNGTVSFGFWLPWVSAVDGNAVTVKIIHEHDQFLQEIPPREFPMA
CCCCCCCEEEEEEEECCCEEEEEEHHHHHHHCCCCEEEEEEEECHHHHHHHCCCCCCCCH
HSVRSPYGDFWSATVPIVGTTPANPGSAWGTAGRYVYRYQINNPNVGILDWIIDPCAREF
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHH
GVGKLSAFTLGYQPYNWSVAEANWRTPELSNLIIYEINIAELGGDLERTRDLMVYLSDLG
CCCCEEEEEECCCCCCCEEECCCCCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHCC
VNAIEIMPLSNVAMSVDWGYLPIGYFGVDERFGKRSDFQKLVDIAHQQGIAVIVDVVYGH
CCEEEEEEECCEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECC
TGVDFPYYDAYNRLQYHENPFMGPFAKDYFSSFGKSTNFGSPFTRDYFFTVNHHWLEVYH
CCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEE
IDGLRYDCVPNYWDGPFGVGYANLVYETYQLTKNKIAQNQRYWNRFNAGAGTSLRLIQYA
ECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQLEGPEEVLRTTYSNSTWQNHTFDAAKAVARGDQRRLADWGLSLGLLGYPEQENTNGDV
HHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCEECCCCCCCCCCCCCCE
ITKTALQYIENHDHERFICNFGLMNPDEAGNPLFLKGDRSHWYMLQPYLIAMLMSKGVPM
EHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCCE
LWQGEEFGESYFLPDFGAGRVGLLRSLRWDYFYDSPGQQLVQLVRKLLRIRRDRSQIRRG
EECCHHHCCEECCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
NYFFFNDWDRYLSKRVLLFARYSNAQYTLVAINIGDIDQSVPFWFPIGGDYNEELHGGDL
CEEEECCHHHHHHCEEEEEEEECCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCC
GLKGITPLQQTWLKVPYHYGRIWTV
CCCCCCHHHHHHHHCCEECCEEEEC
>Mature Secondary Structure 
ASNLLPLSILGAVETNGTVSFGFWLPWVSAVDGNAVTVKIIHEHDQFLQEIPPREFPMA
CCCCCCEEEEEEEECCCEEEEEEHHHHHHHCCCCEEEEEEEECHHHHHHHCCCCCCCCH
HSVRSPYGDFWSATVPIVGTTPANPGSAWGTAGRYVYRYQINNPNVGILDWIIDPCAREF
HHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEECCCCEEHHHHHHHHHHHHH
GVGKLSAFTLGYQPYNWSVAEANWRTPELSNLIIYEINIAELGGDLERTRDLMVYLSDLG
CCCCEEEEEECCCCCCCEEECCCCCCCCCCCEEEEEEEHHHHCCCHHHHHHHHHHHHHCC
VNAIEIMPLSNVAMSVDWGYLPIGYFGVDERFGKRSDFQKLVDIAHQQGIAVIVDVVYGH
CCEEEEEEECCEEEEECCCCEEEEEECCHHHHCCHHHHHHHHHHHHHCCCEEEEEEEECC
TGVDFPYYDAYNRLQYHENPFMGPFAKDYFSSFGKSTNFGSPFTRDYFFTVNHHWLEVYH
CCCCCCCHHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEEEE
IDGLRYDCVPNYWDGPFGVGYANLVYETYQLTKNKIAQNQRYWNRFNAGAGTSLRLIQYA
ECCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
EQLEGPEEVLRTTYSNSTWQNHTFDAAKAVARGDQRRLADWGLSLGLLGYPEQENTNGDV
HHCCCHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHCCEECCCCCCCCCCCCCCE
ITKTALQYIENHDHERFICNFGLMNPDEAGNPLFLKGDRSHWYMLQPYLIAMLMSKGVPM
EHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEEEECCCCCEEEHHHHHHHHHHHCCCCE
LWQGEEFGESYFLPDFGAGRVGLLRSLRWDYFYDSPGQQLVQLVRKLLRIRRDRSQIRRG
EECCHHHCCEECCCCCCCCHHHHHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHCC
NYFFFNDWDRYLSKRVLLFARYSNAQYTLVAINIGDIDQSVPFWFPIGGDYNEELHGGDL
CEEEECCHHHHHHCEEEEEEEECCCEEEEEEEEECCCCCCCCEEEECCCCCCCCCCCCCC
GLKGITPLQQTWLKVPYHYGRIWTV
CCCCCCHHHHHHHHCCEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA