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The map label for this gene is nqrF [H]

Identifier: 114330724

GI number: 114330724

Start: 716393

End: 717088

Strand: Direct

Name: nqrF [H]

Synonym: Neut_0709

Alternate gene names: 114330724

Gene position: 716393-717088 (Clockwise)

Preceding gene: 114330723

Following gene: 114330729

Centisome position: 26.92

GC content: 46.7

Gene sequence:

>696_bases
ATGCCAACCAGTTATGATGTCAGGTTATTGCAGAAAAAGGAAATAGCTGAAGGAACCATGGAATTCACGCTGGAAAAGCC
CAAGGGCTTTGAGTATCGTGCGGGTCAATATGGTGATCTGGTGCTGCCGCCTTCTACCGGGCTGAACGACAGCAATAACA
AACATGGTTTTTCATTCGTTAGTGCACCATTTGAGGACACGTTGCGCATGGCTACCCGCATGCGTGATTCGCTCTATAAA
AGAGCAGCAGCCAAAGTTCCGGAAGGGACCATGGTGCAACTGCTTGCGCTGTGGGGAGATTTCACACTGCAAAAAAACGA
GGCTGTCCCGGTAGTGTTTATCACAGGTGGCATTGGTATTACACCAGTGCGCAGCATTATTGCGCAGGCTACGCATGACA
AGACAAATCACAAAATTACACTGATTTACGCCAACCGCACACCTGTACAGGCTGCTTACACTGATGATCTGAAATGGCTT
GCAGGAGAAAATAAAAATTTTACTTTTGTACCGGTGTATACATCAACCCAAGGTCACGTTAATGCAGAACTCATCAAACA
ACATGTGCCTGATATTATTGCATCGCGCTATTATTTGTCTGGCCCGGAAGGCATGGTTAAGGCCATGCGCGCACTGCTGA
TTGAGATTGGCGCTGATGAAGATAACATCAGAACAGAAGAGTTTGAAGGGTACTAG

Upstream 100 bases:

>100_bases
CCCTGGCCAGCTAATAGCAGCAATCAACACATTTTCCCGCCACACATGCGTGTAAATAGTCAAGCTAGTCTTTAAATTTT
AGAAGAGGTCCAATTCAATT

Downstream 100 bases:

>100_bases
CCCAAGCATATGACGACAAAAAATTCTACTGACATTTATTTATATCTGATCTGGTACTTTGCATCGAAAAAGCTCAATTG
ATCTACCCGCAGGATCATTC

Product: oxidoreductase FAD/NAD(P)-binding subunit

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 231; Mature: 230

Protein sequence:

>231_residues
MPTSYDVRLLQKKEIAEGTMEFTLEKPKGFEYRAGQYGDLVLPPSTGLNDSNNKHGFSFVSAPFEDTLRMATRMRDSLYK
RAAAKVPEGTMVQLLALWGDFTLQKNEAVPVVFITGGIGITPVRSIIAQATHDKTNHKITLIYANRTPVQAAYTDDLKWL
AGENKNFTFVPVYTSTQGHVNAELIKQHVPDIIASRYYLSGPEGMVKAMRALLIEIGADEDNIRTEEFEGY

Sequences:

>Translated_231_residues
MPTSYDVRLLQKKEIAEGTMEFTLEKPKGFEYRAGQYGDLVLPPSTGLNDSNNKHGFSFVSAPFEDTLRMATRMRDSLYK
RAAAKVPEGTMVQLLALWGDFTLQKNEAVPVVFITGGIGITPVRSIIAQATHDKTNHKITLIYANRTPVQAAYTDDLKWL
AGENKNFTFVPVYTSTQGHVNAELIKQHVPDIIASRYYLSGPEGMVKAMRALLIEIGADEDNIRTEEFEGY
>Mature_230_residues
PTSYDVRLLQKKEIAEGTMEFTLEKPKGFEYRAGQYGDLVLPPSTGLNDSNNKHGFSFVSAPFEDTLRMATRMRDSLYKR
AAAKVPEGTMVQLLALWGDFTLQKNEAVPVVFITGGIGITPVRSIIAQATHDKTNHKITLIYANRTPVQAAYTDDLKWLA
GENKNFTFVPVYTSTQGHVNAELIKQHVPDIIASRYYLSGPEGMVKAMRALLIEIGADEDNIRTEEFEGY

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321673, Length=237, Percent_Identity=26.5822784810127, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 25794; Mature: 25662

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSYDVRLLQKKEIAEGTMEFTLEKPKGFEYRAGQYGDLVLPPSTGLNDSNNKHGFSFV
CCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEE
SAPFEDTLRMATRMRDSLYKRAAAKVPEGTMVQLLALWGDFTLQKNEAVPVVFITGGIGI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCH
TPVRSIIAQATHDKTNHKITLIYANRTPVQAAYTDDLKWLAGENKNFTFVPVYTSTQGHV
HHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCEEECCCCCEEEEEEEECCCCCC
NAELIKQHVPDIIASRYYLSGPEGMVKAMRALLIEIGADEDNIRTEEFEGY
CHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTSYDVRLLQKKEIAEGTMEFTLEKPKGFEYRAGQYGDLVLPPSTGLNDSNNKHGFSFV
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCCCCEEE
SAPFEDTLRMATRMRDSLYKRAAAKVPEGTMVQLLALWGDFTLQKNEAVPVVFITGGIGI
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEECCCCEEEEEEECCCCH
TPVRSIIAQATHDKTNHKITLIYANRTPVQAAYTDDLKWLAGENKNFTFVPVYTSTQGHV
HHHHHHHHHHHCCCCCCEEEEEEECCCCCEEEECCCCCEEECCCCCEEEEEEEECCCCCC
NAELIKQHVPDIIASRYYLSGPEGMVKAMRALLIEIGADEDNIRTEEFEGY
CHHHHHHHHHHHHHHCEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA