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The map label for this gene is exoA [H]

Identifier: 114330706

GI number: 114330706

Start: 695773

End: 696546

Strand: Direct

Name: exoA [H]

Synonym: Neut_0691

Alternate gene names: 114330706

Gene position: 695773-696546 (Clockwise)

Preceding gene: 114330705

Following gene: 114330707

Centisome position: 26.15

GC content: 48.45

Gene sequence:

>774_bases
ATGAGAATTATCACTTTGAACCTGAACGGCCTGCGTTCAGCAGCCAGCAAAGGGCTTTTCGACTGGCTGCCTCAACAGGA
GGCGGATGTAATCTGTGTGCAGGAATTAAAGGCACAGCAGAATGATATTAACGGTGTGATGCGCACACCGGATGGCTATA
GTGGTTATTTTCACTGTGCTGTGAAAAAGGGTTACAGCGGGGTCGGTATTTATACCCGTCATGCACCTGATCAGATTGTG
GAGGGAACCGGTATTGCGGAAATCGATGCAGAAGGGCGCTTTTTAAGAGTGGATTTCGGAAATCTGACGGTTATTTCAAT
TTATCTTCCTTCCGGCTCCAGCGGGGAACATCGCCAGGCTGCCAAATTTTTCTTTATGGAGCATTTTTTTCCTCTGTTGC
AATCATTGGCGGAAGGTGGTCGCGAAGTGCTGCTGTGCGGGGATTGGAATATTGCGCATAAGGAAATTGATCTCAAAAAC
TGGCGTTCCAACCAGAAAAATTCTGGATTTTTACCGGAAGAACGCGCCTGGCTGTCTACTGTATTTGACGAGTTAAAACT
GGTGGATGTGTTCCGCACAATCAATCCGGAGCCGGATCAATATACCTGGTGGTCCAATCGCGGTCAGGCGTGGGCAAAGA
ATGTCGGCTGGCGAATAGACTACCAGATTGCTACACCGGGTCTGGCGAGCACAGCAACGGGGGCATCAATTTATAAAACA
GAACGTTTTTCCGACCACGCTCCTCTGCTCATCGATTACGACTTCAATCCATGA

Upstream 100 bases:

>100_bases
TGTCATTCCGAACACGCGCCGTGCACCAGGCTATCCGGCACGGGTTTATTGCAAACCGGATCTCACACGGCGTTGAGAAT
TTATTTACCACTATCATCGC

Downstream 100 bases:

>100_bases
AATTGGATGCATGCATCGGCTGGTTGCATGCGCTGCGTATCTATACTCACCCGAGAGTACTGGGCATGCTGGTGCTTGGG
TTTTCTGCGGGCTTGCCGCT

Product: exodeoxyribonuclease III Xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MRIITLNLNGLRSAASKGLFDWLPQQEADVICVQELKAQQNDINGVMRTPDGYSGYFHCAVKKGYSGVGIYTRHAPDQIV
EGTGIAEIDAEGRFLRVDFGNLTVISIYLPSGSSGEHRQAAKFFFMEHFFPLLQSLAEGGREVLLCGDWNIAHKEIDLKN
WRSNQKNSGFLPEERAWLSTVFDELKLVDVFRTINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGLASTATGASIYKT
ERFSDHAPLLIDYDFNP

Sequences:

>Translated_257_residues
MRIITLNLNGLRSAASKGLFDWLPQQEADVICVQELKAQQNDINGVMRTPDGYSGYFHCAVKKGYSGVGIYTRHAPDQIV
EGTGIAEIDAEGRFLRVDFGNLTVISIYLPSGSSGEHRQAAKFFFMEHFFPLLQSLAEGGREVLLCGDWNIAHKEIDLKN
WRSNQKNSGFLPEERAWLSTVFDELKLVDVFRTINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGLASTATGASIYKT
ERFSDHAPLLIDYDFNP
>Mature_257_residues
MRIITLNLNGLRSAASKGLFDWLPQQEADVICVQELKAQQNDINGVMRTPDGYSGYFHCAVKKGYSGVGIYTRHAPDQIV
EGTGIAEIDAEGRFLRVDFGNLTVISIYLPSGSSGEHRQAAKFFFMEHFFPLLQSLAEGGREVLLCGDWNIAHKEIDLKN
WRSNQKNSGFLPEERAWLSTVFDELKLVDVFRTINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGLASTATGASIYKT
ERFSDHAPLLIDYDFNP

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=254, Percent_Identity=34.251968503937, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI18375503, Length=254, Percent_Identity=34.251968503937, Blast_Score=164, Evalue=8e-41,
Organism=Homo sapiens, GI18375501, Length=254, Percent_Identity=34.251968503937, Blast_Score=164, Evalue=8e-41,
Organism=Escherichia coli, GI1788046, Length=266, Percent_Identity=30.8270676691729, Blast_Score=112, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI71989536, Length=263, Percent_Identity=30.7984790874525, Blast_Score=130, Evalue=9e-31,
Organism=Drosophila melanogaster, GI221330655, Length=253, Percent_Identity=31.2252964426877, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI17136678, Length=253, Percent_Identity=31.2252964426877, Blast_Score=125, Evalue=4e-29,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29012; Mature: 29012

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIITLNLNGLRSAASKGLFDWLPQQEADVICVQELKAQQNDINGVMRTPDGYSGYFHCA
CEEEEEECCHHHHHHHCCHHHHCCCCCCCEEEHHHHHHHHCCCCCEEECCCCCCCEEEEE
VKKGYSGVGIYTRHAPDQIVEGTGIAEIDAEGRFLRVDFGNLTVISIYLPSGSSGEHRQA
EECCCCCCEEEECCCCHHHHCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCCHHHH
AKFFFMEHFFPLLQSLAEGGREVLLCGDWNIAHKEIDLKNWRSNQKNSGFLPEERAWLST
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECHHHCCCCCCCCCCCCHHHHHHHH
VFDELKLVDVFRTINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGLASTATGASIYKT
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCEEEE
ERFSDHAPLLIDYDFNP
CCCCCCCCEEEEECCCC
>Mature Secondary Structure
MRIITLNLNGLRSAASKGLFDWLPQQEADVICVQELKAQQNDINGVMRTPDGYSGYFHCA
CEEEEEECCHHHHHHHCCHHHHCCCCCCCEEEHHHHHHHHCCCCCEEECCCCCCCEEEEE
VKKGYSGVGIYTRHAPDQIVEGTGIAEIDAEGRFLRVDFGNLTVISIYLPSGSSGEHRQA
EECCCCCCEEEECCCCHHHHCCCCEEEECCCCCEEEEECCCEEEEEEECCCCCCCCHHHH
AKFFFMEHFFPLLQSLAEGGREVLLCGDWNIAHKEIDLKNWRSNQKNSGFLPEERAWLST
HHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEEECHHHCCCCCCCCCCCCHHHHHHHH
VFDELKLVDVFRTINPEPDQYTWWSNRGQAWAKNVGWRIDYQIATPGLASTATGASIYKT
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHCCCEEEEEEEECCCCCCCCCCCCEEEE
ERFSDHAPLLIDYDFNP
CCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]