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The map label for this gene is mpl [H]

Identifier: 114330666

GI number: 114330666

Start: 660666

End: 662048

Strand: Reverse

Name: mpl [H]

Synonym: Neut_0651

Alternate gene names: 114330666

Gene position: 662048-660666 (Counterclockwise)

Preceding gene: 114330668

Following gene: 114330664

Centisome position: 24.88

GC content: 53.22

Gene sequence:

>1383_bases
ATGCATATTCACATTCTTGGAATCTGCGGTACCTTCATGGGCGGACTGGCGGTACTGGCACGCGCTGCGGGTCATACCGT
CACCGGCTGTGATGCCAACGTCTACCCGCCAATGAGCACACAGTTACGCGATCAAAATATCGAATTGATCGAAGGCTATA
GCACCGATCAGATTAAGCTGGCACCTGATTTATTCGTAGTTGGCAACGTCGTCACACGCGGCAACCCGTTGATGGAGGCC
ATTCTCGATGCGCATCTGCCCTACACATCCGGCCCGCAATGGCTGGCACAGCACCTGCTGCATAACCGCTGGGTACTTGC
CGTTGCCGGTACGCATGGCAAAACCACCACCAGTTCAATGCTTGCCTGGATTCTGGAATACGCCGGGCTTGCCCCCGGTT
TCCTCATCGGCGGCGTCCCGGAGAATTTTGGCCTGTCTGCCCGTCTGGGGCATATCGACGGAGAACAAACTCTCTCTCCT
TTCTTTGTCATCGAAGCGGATGAATACGACACCGCCTTCTTCGACAAGCGCTCCAAATTTGTGCACTATCAGCCCAAAAC
TGCGGTACTCAATAACCTTGAATTTGATCACGCGGATATTTTCCCGGATCTGGCCGCGATCGAGCGTCAGTTCCATCACC
TTGTCCGCATCGTTCCGCGCAATGGATTGATTATTGTCAACGGCTGTGATGAAACCCTGCAGCGCGTGTTGCATCAAGGA
TGCTGGACGCCTGTAGAAAAATTCGGGGTGGATGAAGGCTGGCAAATCACCACGGAACACGATCAAAGCCAGTCAATTCG
TTTCCAAGGCGAACCGCAGGGAATATTGCACTGGGAGTTACTGGGAGAACATAACCGCATGAATGCACTGGCAGCGCTGG
CTGCAGCGCGTCACGCCGGCGTACCGGCACCACTTGGCATTGAAGCCTTATCCCACTTTAAAAATGTGAAACGCCGCATG
GAGCTGCGCGGGATTATTCATGATATTCACATCTATGATGATTTTGCGCATCACCCCACCGCTATTCAAACAACCTTGGC
AGGGCTGCGCGACAAGATTGGTTCGGCACGCATCCTCGCTGTGCTGGAGCCACGCTCCAATACCATGAAACTTGGTGTCT
GGCAGGACAGCCTCGCTGCCAGTCTGAATCAGGCCGATCAGGTATTCTGCTATGCCCATCAGATGGGCTGGGACGTAGCT
TCAGCCCTCACTTCCCTCAGCAATAAAGCTGACATTCACCATGATCTATCTGCCATGATCAGCGCCATTACTACTGCGGC
ACAACCAGGTGATCATATCTTGATCATGAGTAACGGCAGCTTTAATGGCCTGTACGATAAACTGCTTGATGCCTTGCGCA
ACCAGATTAACCTGTCCTCCTGA

Upstream 100 bases:

>100_bases
CGGAAACCAGCGTAGCACGGATACATCCCCCGGTCACGCCTCGCGTATAATCACCCGCTCAACCGATTGCCTCCTTCACA
ATTACCCTGCCGGAACATTT

Downstream 100 bases:

>100_bases
CACTCATAAAAAAACCCCGCCAAAACTTAGGCGGGGTATAAAGGCTTCGTCACAAAATCTGTTGCTTGGGCTCCTATTTT
GCTCGAAGCGAGAAGAGAGA

Product: UDP-N-acetylmuramate

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MHIHILGICGTFMGGLAVLARAAGHTVTGCDANVYPPMSTQLRDQNIELIEGYSTDQIKLAPDLFVVGNVVTRGNPLMEA
ILDAHLPYTSGPQWLAQHLLHNRWVLAVAGTHGKTTTSSMLAWILEYAGLAPGFLIGGVPENFGLSARLGHIDGEQTLSP
FFVIEADEYDTAFFDKRSKFVHYQPKTAVLNNLEFDHADIFPDLAAIERQFHHLVRIVPRNGLIIVNGCDETLQRVLHQG
CWTPVEKFGVDEGWQITTEHDQSQSIRFQGEPQGILHWELLGEHNRMNALAALAAARHAGVPAPLGIEALSHFKNVKRRM
ELRGIIHDIHIYDDFAHHPTAIQTTLAGLRDKIGSARILAVLEPRSNTMKLGVWQDSLAASLNQADQVFCYAHQMGWDVA
SALTSLSNKADIHHDLSAMISAITTAAQPGDHILIMSNGSFNGLYDKLLDALRNQINLSS

Sequences:

>Translated_460_residues
MHIHILGICGTFMGGLAVLARAAGHTVTGCDANVYPPMSTQLRDQNIELIEGYSTDQIKLAPDLFVVGNVVTRGNPLMEA
ILDAHLPYTSGPQWLAQHLLHNRWVLAVAGTHGKTTTSSMLAWILEYAGLAPGFLIGGVPENFGLSARLGHIDGEQTLSP
FFVIEADEYDTAFFDKRSKFVHYQPKTAVLNNLEFDHADIFPDLAAIERQFHHLVRIVPRNGLIIVNGCDETLQRVLHQG
CWTPVEKFGVDEGWQITTEHDQSQSIRFQGEPQGILHWELLGEHNRMNALAALAAARHAGVPAPLGIEALSHFKNVKRRM
ELRGIIHDIHIYDDFAHHPTAIQTTLAGLRDKIGSARILAVLEPRSNTMKLGVWQDSLAASLNQADQVFCYAHQMGWDVA
SALTSLSNKADIHHDLSAMISAITTAAQPGDHILIMSNGSFNGLYDKLLDALRNQINLSS
>Mature_460_residues
MHIHILGICGTFMGGLAVLARAAGHTVTGCDANVYPPMSTQLRDQNIELIEGYSTDQIKLAPDLFVVGNVVTRGNPLMEA
ILDAHLPYTSGPQWLAQHLLHNRWVLAVAGTHGKTTTSSMLAWILEYAGLAPGFLIGGVPENFGLSARLGHIDGEQTLSP
FFVIEADEYDTAFFDKRSKFVHYQPKTAVLNNLEFDHADIFPDLAAIERQFHHLVRIVPRNGLIIVNGCDETLQRVLHQG
CWTPVEKFGVDEGWQITTEHDQSQSIRFQGEPQGILHWELLGEHNRMNALAALAAARHAGVPAPLGIEALSHFKNVKRRM
ELRGIIHDIHIYDDFAHHPTAIQTTLAGLRDKIGSARILAVLEPRSNTMKLGVWQDSLAASLNQADQVFCYAHQMGWDVA
SALTSLSNKADIHHDLSAMISAITTAAQPGDHILIMSNGSFNGLYDKLLDALRNQINLSS

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=461, Percent_Identity=56.8329718004338, Blast_Score=510, Evalue=1e-146,
Organism=Escherichia coli, GI1786279, Length=339, Percent_Identity=27.1386430678466, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50614; Mature: 50614

Theoretical pI: Translated: 6.38; Mature: 6.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAVLARAAGHTVTGCDANVYPPMSTQLRDQNIELIEGYSTDQIKL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCCCCEEEECCCCCEEEE
APDLFVVGNVVTRGNPLMEAILDAHLPYTSGPQWLAQHLLHNRWVLAVAGTHGKTTTSSM
CCCEEEECCHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEYAGLAPGFLIGGVPENFGLSARLGHIDGEQTLSPFFVIEADEYDTAFFDKRSKF
HHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCHHHHHCCCCE
VHYQPKTAVLNNLEFDHADIFPDLAAIERQFHHLVRIVPRNGLIIVNGCDETLQRVLHQG
EEECCHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHC
CWTPVEKFGVDEGWQITTEHDQSQSIRFQGEPQGILHWELLGEHNRMNALAALAAARHAG
CCCCHHHCCCCCCCEEEECCCCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
VPAPLGIEALSHFKNVKRRMELRGIIHDIHIYDDFAHHPTAIQTTLAGLRDKIGSARILA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
VLEPRSNTMKLGVWQDSLAASLNQADQVFCYAHQMGWDVASALTSLSNKADIHHDLSAMI
EECCCCCEEEEEECHHHHHHHHCCHHHEEEEHHHHCHHHHHHHHHHCCCCHHHHHHHHHH
SAITTAAQPGDHILIMSNGSFNGLYDKLLDALRNQINLSS
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAVLARAAGHTVTGCDANVYPPMSTQLRDQNIELIEGYSTDQIKL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCHHCCCCCEEEECCCCCEEEE
APDLFVVGNVVTRGNPLMEAILDAHLPYTSGPQWLAQHLLHNRWVLAVAGTHGKTTTSSM
CCCEEEECCHHHCCCHHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEYAGLAPGFLIGGVPENFGLSARLGHIDGEQTLSPFFVIEADEYDTAFFDKRSKF
HHHHHHHHCCCCCCEECCCCCCCCCCEEECCCCCCCCCCCEEEEECCCCCHHHHHCCCCE
VHYQPKTAVLNNLEFDHADIFPDLAAIERQFHHLVRIVPRNGLIIVNGCDETLQRVLHQG
EEECCHHHHHCCCCCCHHHHCHHHHHHHHHHHHHHEEECCCCEEEEECCHHHHHHHHHHC
CWTPVEKFGVDEGWQITTEHDQSQSIRFQGEPQGILHWELLGEHNRMNALAALAAARHAG
CCCCHHHCCCCCCCEEEECCCCCCCEEECCCCCCEEEEEEECCCCHHHHHHHHHHHHHCC
VPAPLGIEALSHFKNVKRRMELRGIIHDIHIYDDFAHHPTAIQTTLAGLRDKIGSARILA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHCCCEEEEE
VLEPRSNTMKLGVWQDSLAASLNQADQVFCYAHQMGWDVASALTSLSNKADIHHDLSAMI
EECCCCCEEEEEECHHHHHHHHCCHHHEEEEHHHHCHHHHHHHHHHCCCCHHHHHHHHHH
SAITTAAQPGDHILIMSNGSFNGLYDKLLDALRNQINLSS
HHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]