Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 114330527

GI number: 114330527

Start: 527878

End: 529572

Strand: Direct

Name: epsE [H]

Synonym: Neut_0508

Alternate gene names: 114330527

Gene position: 527878-529572 (Clockwise)

Preceding gene: 114330526

Following gene: 114330528

Centisome position: 19.84

GC content: 52.74

Gene sequence:

>1695_bases
TTGACGCAGACGCAGGTCGCCCTGCTGGACCAACCGGTAATTGATGCAGCCCAACTTCGCCAGGCACGGGAGCAGGCTGT
CATCCATGGTACGTCGCTGATCAAGGTGCTGGAAGAAACCTGGCAAGGTACTCCGGATGAACTGATAGTCGCACTAAGTA
GAGCACTGCGTGTGCCGATGCTCTCCATGAGCGATTTGCATGCGTTATATCCGGCTTTTGACAAGTTGCCTTTTGCTCAG
GCGGTCAAGCATGAGTGCGTGTTGTTGCAAAAACCGGATCAGTCGTATCTGCTTGCCGTCAGTGATCCATTCCGGCCGAG
CCTGCGTGACTGGGTGGAAGAATATGTGCCGGTCAATGCGCTGTGGCATCTGGTACATCCAGCAGATCTGGCTGCCTTTT
TTGCGCAGCAGGAACAGAGTATGCGCGCGATGGATAATGTTCTGACTTCTGCCCGGGAAAACCTGATTCAAACTGGTATT
GAGGAGTTGTCGCTCAAAACGATACATGAAGGTACCAGCCAGGTGGTGCGCCTGGTGCATTCAACACTGTACGATGCACA
TAAATCTCAGGCAAGCGATATCCATCTGGAAACCACGCCAGGTGCGCTGTCCATCAGATACCGGATCGACGGCGTACTCA
CCAGCATTGGTACGATGCAGGGGACGGATCTGGCTGAACAGGTGATTTCCCGCATCAAGGTAATGTCGGATCTGGATATT
GCCGAACGCCGCATTCCGCAGGATGGTCGCTTCAAGGTATCCATCCAGGGCAGGGAAATTGATTTTCGCGTGTCGGTCAT
GCCCAGCATTTTTGGGGAAGATGCGGTGCTGCGAATTCTGGACCGTCAGGCGCTGACCGATCATATTGAAGGGTTGACAC
TGAATCAGTTGGGATTCAATGTCGCTGCTATTGCCAGCATACGTCGTCTCAGCGCAGAACCATATGGCATGCTGCTGGTA
ACCGGGCCGACCGGCAGCGGCAAAACAACTTCGCTCTATGCTGCTATTTCTGAAGTTAACCACGGCCAGGACAAAATCAT
CACTATCGAAGATCCGATCGAATACCAATTGCCGGGTGTGCTGCAGATACCCGTTAACGAAAAAAAAGGATTGACATTTG
CCCGAGGGCTGCGTTCAATTTTGCGGCATGACCCCGACAAGATCATGGTGGGAGAAATCCGTGATGCTGAAACCGCACAA
ATCGCTATTCAGGCTGCATTGACGGGGCACTTGGTGTTTACCACAGTGCATGCCAACAATGTATTTGATGTAATCGGCCG
GTTTGCGCACATGGGAGTGGATCCCTACAGCTTTGTTTCGGCACTGAACGGTATCGTGGCACAGCGGCTGGTGCGTTTGT
TATGCCCAAATTGCATGGTTTCTGAGCAACCGGATCATCAGATGATCGAGGAATCGGGGATTCCTTTGGAACAGGCGGAA
CAGTTTGATTTCAACTCTGCAAAAGGTTGCGGGCACTGCCGAGGCAGTGGTTATCGCGGGCGCAGCGCCATTGCTGAGAT
CTTGCTGTTGAACGATGAAATCCGTGAGCTGATTATCGCGCAGGCACCTATTCGTCGGATCAAGGAAGCTGCCCGGCTGA
ATGGCACCCATTTTTTGCGTGAAGCAGCGCTGGATATGGTGAAATCCGGGCAAACCAGCTTACAGGAGGCTAATCGTGTC
ACGGTTGTGGCGTAA

Upstream 100 bases:

>100_bases
AACTGATCGAGCCGCTGTTGATGGCAGTAATCGGAACTGTCATCGGCGGTATCATCATTCTCATGTATCTGCCAATTTTT
GAGCTGGCCGGGAGTATCAA

Downstream 100 bases:

>100_bases
CTGTATGCAGGTTTTTTTGGCACCCGGCCGGGTCAATCTGGTGGGTTTTGCCCGTGGCATCAAGCCCATGCAGCGCTTCA
GGCAGTCGGGTACTTGTGGT

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 564; Mature: 563

Protein sequence:

>564_residues
MTQTQVALLDQPVIDAAQLRQAREQAVIHGTSLIKVLEETWQGTPDELIVALSRALRVPMLSMSDLHALYPAFDKLPFAQ
AVKHECVLLQKPDQSYLLAVSDPFRPSLRDWVEEYVPVNALWHLVHPADLAAFFAQQEQSMRAMDNVLTSARENLIQTGI
EELSLKTIHEGTSQVVRLVHSTLYDAHKSQASDIHLETTPGALSIRYRIDGVLTSIGTMQGTDLAEQVISRIKVMSDLDI
AERRIPQDGRFKVSIQGREIDFRVSVMPSIFGEDAVLRILDRQALTDHIEGLTLNQLGFNVAAIASIRRLSAEPYGMLLV
TGPTGSGKTTSLYAAISEVNHGQDKIITIEDPIEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDAETAQ
IAIQAALTGHLVFTTVHANNVFDVIGRFAHMGVDPYSFVSALNGIVAQRLVRLLCPNCMVSEQPDHQMIEESGIPLEQAE
QFDFNSAKGCGHCRGSGYRGRSAIAEILLLNDEIRELIIAQAPIRRIKEAARLNGTHFLREAALDMVKSGQTSLQEANRV
TVVA

Sequences:

>Translated_564_residues
MTQTQVALLDQPVIDAAQLRQAREQAVIHGTSLIKVLEETWQGTPDELIVALSRALRVPMLSMSDLHALYPAFDKLPFAQ
AVKHECVLLQKPDQSYLLAVSDPFRPSLRDWVEEYVPVNALWHLVHPADLAAFFAQQEQSMRAMDNVLTSARENLIQTGI
EELSLKTIHEGTSQVVRLVHSTLYDAHKSQASDIHLETTPGALSIRYRIDGVLTSIGTMQGTDLAEQVISRIKVMSDLDI
AERRIPQDGRFKVSIQGREIDFRVSVMPSIFGEDAVLRILDRQALTDHIEGLTLNQLGFNVAAIASIRRLSAEPYGMLLV
TGPTGSGKTTSLYAAISEVNHGQDKIITIEDPIEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDAETAQ
IAIQAALTGHLVFTTVHANNVFDVIGRFAHMGVDPYSFVSALNGIVAQRLVRLLCPNCMVSEQPDHQMIEESGIPLEQAE
QFDFNSAKGCGHCRGSGYRGRSAIAEILLLNDEIRELIIAQAPIRRIKEAARLNGTHFLREAALDMVKSGQTSLQEANRV
TVVA
>Mature_563_residues
TQTQVALLDQPVIDAAQLRQAREQAVIHGTSLIKVLEETWQGTPDELIVALSRALRVPMLSMSDLHALYPAFDKLPFAQA
VKHECVLLQKPDQSYLLAVSDPFRPSLRDWVEEYVPVNALWHLVHPADLAAFFAQQEQSMRAMDNVLTSARENLIQTGIE
ELSLKTIHEGTSQVVRLVHSTLYDAHKSQASDIHLETTPGALSIRYRIDGVLTSIGTMQGTDLAEQVISRIKVMSDLDIA
ERRIPQDGRFKVSIQGREIDFRVSVMPSIFGEDAVLRILDRQALTDHIEGLTLNQLGFNVAAIASIRRLSAEPYGMLLVT
GPTGSGKTTSLYAAISEVNHGQDKIITIEDPIEYQLPGVLQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDAETAQI
AIQAALTGHLVFTTVHANNVFDVIGRFAHMGVDPYSFVSALNGIVAQRLVRLLCPNCMVSEQPDHQMIEESGIPLEQAEQ
FDFNSAKGCGHCRGSGYRGRSAIAEILLLNDEIRELIIAQAPIRRIKEAARLNGTHFLREAALDMVKSGQTSLQEANRVT
VVA

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=394, Percent_Identity=46.1928934010152, Blast_Score=332, Evalue=3e-92,
Organism=Escherichia coli, GI1786296, Length=386, Percent_Identity=40.6735751295337, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI87082188, Length=145, Percent_Identity=41.3793103448276, Blast_Score=102, Evalue=8e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62310; Mature: 62179

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTQVALLDQPVIDAAQLRQAREQAVIHGTSLIKVLEETWQGTPDELIVALSRALRVPM
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
LSMSDLHALYPAFDKLPFAQAVKHECVLLQKPDQSYLLAVSDPFRPSLRDWVEEYVPVNA
CCHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCHHH
LWHLVHPADLAAFFAQQEQSMRAMDNVLTSARENLIQTGIEELSLKTIHEGTSQVVRLVH
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLYDAHKSQASDIHLETTPGALSIRYRIDGVLTSIGTMQGTDLAEQVISRIKVMSDLDI
HHHHHHHCCCCCCEEEEECCCEEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
AERRIPQDGRFKVSIQGREIDFRVSVMPSIFGEDAVLRILDRQALTDHIEGLTLNQLGFN
HHHHCCCCCCEEEEEECCEEEEEEEECHHHHCCHHHHHHHHHHHHHHHHCCCEEHHHCCH
VAAIASIRRLSAEPYGMLLVTGPTGSGKTTSLYAAISEVNHGQDKIITIEDPIEYQLPGV
HHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
LQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDAETAQIAIQAALTGHLVFTTVHANN
EEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHEEHHHHCCEEEEEEEECCH
VFDVIGRFAHMGVDPYSFVSALNGIVAQRLVRLLCPNCMVSEQPDHQMIEESGIPLEQAE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
QFDFNSAKGCGHCRGSGYRGRSAIAEILLLNDEIRELIIAQAPIRRIKEAARLNGTHFLR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EAALDMVKSGQTSLQEANRVTVVA
HHHHHHHHCCCHHHHHCCCEEEEC
>Mature Secondary Structure 
TQTQVALLDQPVIDAAQLRQAREQAVIHGTSLIKVLEETWQGTPDELIVALSRALRVPM
CCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCC
LSMSDLHALYPAFDKLPFAQAVKHECVLLQKPDQSYLLAVSDPFRPSLRDWVEEYVPVNA
CCHHHHHHHHHHHHCCCHHHHHCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHCCHHH
LWHLVHPADLAAFFAQQEQSMRAMDNVLTSARENLIQTGIEELSLKTIHEGTSQVVRLVH
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
STLYDAHKSQASDIHLETTPGALSIRYRIDGVLTSIGTMQGTDLAEQVISRIKVMSDLDI
HHHHHHHCCCCCCEEEEECCCEEEEEEEEHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCH
AERRIPQDGRFKVSIQGREIDFRVSVMPSIFGEDAVLRILDRQALTDHIEGLTLNQLGFN
HHHHCCCCCCEEEEEECCEEEEEEEECHHHHCCHHHHHHHHHHHHHHHHCCCEEHHHCCH
VAAIASIRRLSAEPYGMLLVTGPTGSGKTTSLYAAISEVNHGQDKIITIEDPIEYQLPGV
HHHHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCE
LQIPVNEKKGLTFARGLRSILRHDPDKIMVGEIRDAETAQIAIQAALTGHLVFTTVHANN
EEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCHHHHEEHHHHCCEEEEEEEECCH
VFDVIGRFAHMGVDPYSFVSALNGIVAQRLVRLLCPNCMVSEQPDHQMIEESGIPLEQAE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCHHHHH
QFDFNSAKGCGHCRGSGYRGRSAIAEILLLNDEIRELIIAQAPIRRIKEAARLNGTHFLR
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EAALDMVKSGQTSLQEANRVTVVA
HHHHHHHHCCCHHHHHCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]