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The map label for this gene is gltX

Identifier: 114330518

GI number: 114330518

Start: 513261

End: 514652

Strand: Direct

Name: gltX

Synonym: Neut_0499

Alternate gene names: 114330518

Gene position: 513261-514652 (Clockwise)

Preceding gene: 114330510

Following gene: 114330519

Centisome position: 19.29

GC content: 50.65

Gene sequence:

>1392_bases
ATGGTAAAAACACGTTTTGCTCCCAGTCCAACCGGTTACCTGCATATTGGCGGTGCACGCACTGCGCTTTTTTCCTGGGC
GTTTGCCCGGAAACAAGGTGGAAAATTTATTCTGAGAATCGAGGATACTGATCTTGAACGTTCTACCCAGCAATCTGTGC
AGGCGATTCTCGATGGGATGGCCTGGCTGGGGCTTGATTATGATGAGGGGCCGTATTACCAGATGCAGCGGTTGAGTCGC
TACCAGGAAGTTGCGGAGCTATTGCTCAAGCAGGGACTTGCTTATCGTTGTTATGCCAGTAAGGAAGAGCTGGATGCGCT
GCGTGAACAGCAGCGTTTGGCCGGGTTGAAGCCGCGTTATGATGGCCGCTGGCGCGATAGCAAGCAGACTCCGCCAGCTG
GTGTCACGCCGGTAGTGCGCTTTAAAACGCCACGTGAAGGGTATGCGGTATTTGATGATCTGGTTAAGGGGCGGATCGCG
GTAGCCAATCATGAGCTGGATGATCTGGTATTAATGCGAAGTGACGGCACGCCTACCTACAATTTTGGAGTGGTGCTGGA
TGATCTTGATATGGGTGTTACACACGTTATCCGTGGGGATGATCATGTCAACAATACTCCTCGTCAAATCAATATCCTCA
AGGCACTGGGGGCGATCATTCCACAATACGCACATGTACCGATGATTCTTGGCGCAGATGGTGAGCGTTTATCTAAACGG
CATGGCGCGGTATCCGTGATGCATTACCGGGATCAGGGATACCTGCCGGAAGCCTTGATCAACTATCTGGCACGACTCGG
GTGGTCACACGGAGATGAGGAAATTTTCAGCCAGGAACAATTAGTTGAATGGTTTGATCTGGCGGCAATCAATCGTTCGC
CGGCGAAATTTAATCCGGAAAAATTGACCTGGCTGAACCAGCATTATCTGAAAATTGCCGAGGATGCCCGGTTGATGGAA
CTGGTTACACCCATTCTGCTGGGAAGAAAATACAGCCTGCCGGGAGAGGAAAATATGTTTAAAATCATCAACCTGCTTAA
AGAGCGTGTCAGCACGATAGAAGAGCTGGCCGACGCATCTGCTTATTTTTTTCAAGCCGTCGAGCCTGCCGAAGCGCTCA
GAACGCAGTATTTCACCGTTGAAATTCGTCCGGTTCTGGAATACCTGATTGATCGTCTGGCGCAGATTGAATGGAAGCGA
GAAGCGATTCACCAAGAAATTAAACAGACCGTTTCCTCACATAAGCTGAAGTTTCCCAACTTGGCGATGCCGCTGCGTGT
CATGGTCACGGGTGAAGCACAAACTCCGGCGATTGACGCAGTGCTGGAGCTGATTGGTAAAGAAGAAACTCTGCACCGTT
TGCGTGACCAGCTGGATGCGTTTCCGCAGTAA

Upstream 100 bases:

>100_bases
CAGCTCAAAAAATATTTCCTCCAGAGGAATCAGCAAGGATTTCCTGGTATGGAAACAACCGTGTAAACGGTTGTGTATAC
AACTTCTAATATTTAGTCAG

Downstream 100 bases:

>100_bases
GCATACAGCTGCCGTATTTATTTTGACTAAAAATTCCCGTTTGAAGAGCTGTTATGACAATGTCTGATCGTGCTGATTTG
CTGAAACAATTACTTGCCGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFILRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLSR
YQEVAELLLKQGLAYRCYASKEELDALREQQRLAGLKPRYDGRWRDSKQTPPAGVTPVVRFKTPREGYAVFDDLVKGRIA
VANHELDDLVLMRSDGTPTYNFGVVLDDLDMGVTHVIRGDDHVNNTPRQINILKALGAIIPQYAHVPMILGADGERLSKR
HGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSQEQLVEWFDLAAINRSPAKFNPEKLTWLNQHYLKIAEDARLME
LVTPILLGRKYSLPGEENMFKIINLLKERVSTIEELADASAYFFQAVEPAEALRTQYFTVEIRPVLEYLIDRLAQIEWKR
EAIHQEIKQTVSSHKLKFPNLAMPLRVMVTGEAQTPAIDAVLELIGKEETLHRLRDQLDAFPQ

Sequences:

>Translated_463_residues
MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFILRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLSR
YQEVAELLLKQGLAYRCYASKEELDALREQQRLAGLKPRYDGRWRDSKQTPPAGVTPVVRFKTPREGYAVFDDLVKGRIA
VANHELDDLVLMRSDGTPTYNFGVVLDDLDMGVTHVIRGDDHVNNTPRQINILKALGAIIPQYAHVPMILGADGERLSKR
HGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSQEQLVEWFDLAAINRSPAKFNPEKLTWLNQHYLKIAEDARLME
LVTPILLGRKYSLPGEENMFKIINLLKERVSTIEELADASAYFFQAVEPAEALRTQYFTVEIRPVLEYLIDRLAQIEWKR
EAIHQEIKQTVSSHKLKFPNLAMPLRVMVTGEAQTPAIDAVLELIGKEETLHRLRDQLDAFPQ
>Mature_463_residues
MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFILRIEDTDLERSTQQSVQAILDGMAWLGLDYDEGPYYQMQRLSR
YQEVAELLLKQGLAYRCYASKEELDALREQQRLAGLKPRYDGRWRDSKQTPPAGVTPVVRFKTPREGYAVFDDLVKGRIA
VANHELDDLVLMRSDGTPTYNFGVVLDDLDMGVTHVIRGDDHVNNTPRQINILKALGAIIPQYAHVPMILGADGERLSKR
HGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSQEQLVEWFDLAAINRSPAKFNPEKLTWLNQHYLKIAEDARLME
LVTPILLGRKYSLPGEENMFKIINLLKERVSTIEELADASAYFFQAVEPAEALRTQYFTVEIRPVLEYLIDRLAQIEWKR
EAIHQEIKQTVSSHKLKFPNLAMPLRVMVTGEAQTPAIDAVLELIGKEETLHRLRDQLDAFPQ

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=486, Percent_Identity=31.4814814814815, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI4826960, Length=299, Percent_Identity=26.0869565217391, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI62241042, Length=235, Percent_Identity=26.8085106382979, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1788743, Length=461, Percent_Identity=52.2776572668113, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI226510925, Length=281, Percent_Identity=33.0960854092527, Blast_Score=129, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=31.4465408805031, Blast_Score=224, Evalue=6e-59,
Organism=Saccharomyces cerevisiae, GI6324540, Length=500, Percent_Identity=33, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324742, Length=133, Percent_Identity=31.5789473684211, Blast_Score=74, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6321192, Length=215, Percent_Identity=27.906976744186, Blast_Score=70, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=32.9218106995885, Blast_Score=269, Evalue=4e-72,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_NITEC (Q0AIP6)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746740.1
- ProteinModelPortal:   Q0AIP6
- SMR:   Q0AIP6
- STRING:   Q0AIP6
- GeneID:   4272478
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0499
- NMPDR:   fig|335283.3.peg.1376
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- PhylomeDB:   Q0AIP6
- ProtClustDB:   PRK01406
- BioCyc:   NEUT335283:NEUT_0499-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52848; Mature: 52848

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFILRIEDTDLERSTQQSVQAILDGM
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHH
AWLGLDYDEGPYYQMQRLSRYQEVAELLLKQGLAYRCYASKEELDALREQQRLAGLKPRY
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
DGRWRDSKQTPPAGVTPVVRFKTPREGYAVFDDLVKGRIAVANHELDDLVLMRSDGTPTY
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCCE
NFGVVLDDLDMGVTHVIRGDDHVNNTPRQINILKALGAIIPQYAHVPMILGADGERLSKR
EEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHH
HGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSQEQLVEWFDLAAINRSPAKFNPE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
KLTWLNQHYLKIAEDARLMELVTPILLGRKYSLPGEENMFKIINLLKERVSTIEELADAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AYFFQAVEPAEALRTQYFTVEIRPVLEYLIDRLAQIEWKREAIHQEIKQTVSSHKLKFPN
HHHHHHCCHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAMPLRVMVTGEAQTPAIDAVLELIGKEETLHRLRDQLDAFPQ
CCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVKTRFAPSPTGYLHIGGARTALFSWAFARKQGGKFILRIEDTDLERSTQQSVQAILDGM
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHH
AWLGLDYDEGPYYQMQRLSRYQEVAELLLKQGLAYRCYASKEELDALREQQRLAGLKPRY
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHCCCCCCC
DGRWRDSKQTPPAGVTPVVRFKTPREGYAVFDDLVKGRIAVANHELDDLVLMRSDGTPTY
CCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHCCEEEEECCCCCCEEEEECCCCCCE
NFGVVLDDLDMGVTHVIRGDDHVNNTPRQINILKALGAIIPQYAHVPMILGADGERLSKR
EEEEEEECCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHH
HGAVSVMHYRDQGYLPEALINYLARLGWSHGDEEIFSQEQLVEWFDLAAINRSPAKFNPE
CCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHH
KLTWLNQHYLKIAEDARLMELVTPILLGRKYSLPGEENMFKIINLLKERVSTIEELADAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AYFFQAVEPAEALRTQYFTVEIRPVLEYLIDRLAQIEWKREAIHQEIKQTVSSHKLKFPN
HHHHHHCCHHHHHHHHEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
LAMPLRVMVTGEAQTPAIDAVLELIGKEETLHRLRDQLDAFPQ
CCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA