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The map label for this gene is pyrB

Identifier: 114330471

GI number: 114330471

Start: 460498

End: 461448

Strand: Direct

Name: pyrB

Synonym: Neut_0451

Alternate gene names: 114330471

Gene position: 460498-461448 (Clockwise)

Preceding gene: 114330470

Following gene: 114330472

Centisome position: 17.31

GC content: 48.48

Gene sequence:

>951_bases
ATGAGTATACATCCGCAGTTGAATAAAAATGGGGAGCTGCAGCATCTGCTTACGACAGAGGGGTTACCTGTTACGATTTT
GCGCCATATCCTTGATACAGCGGAATCATTCACTGGTGTGACAGAGCGGGATGTCAAGAAAATTCCTCTGCTGCGAGGCA
AATCGGTATTCAACCTGTTTTTTGAGCCGAGTACCCGCACTCGCACAACTTTCGAAATTGCGGCAAAACGCCTTTCAGCG
GATGTAATCAATCTTAGTATGGCAGTTTCATCCCAGACCAAAGGGGAAACTTTACTGGATACGGTGAACAACCTGTCTGC
CATGCATGCAGATATGTTTGTGGTACGCCACAACCAGAGTGGTGCAGCGCATCTGATCGCTCGTCATGTCGGCCCGGAAA
TTCATGTTATTAATGCCGGTGACGGATGGCATGCGCACCCTACGCAGGCATTGCTTGATATGTTTACCATCCGTCGTTAC
AAACAGGATTTTCATACACTGCGTGTGGCAATTATTGGAGATATTCTGCATTCCCGAGTGGCGCGTTCACAGATTCACGC
ACTGACAACACTGGGAGTACCGGAAATCCGTGTTATTGCACCCAAGACGTTGTTGCCGGCCCGGGTTGAGCGGCTTGGTG
TGCATGTTTACCACAACATGACACAAGGCTTGAAAGATGTAGATGTGCTGATGATGCTGCGATTGCAGCATGAACGTATG
GAATCTGCGCATTTACCCAGCACCGAAGAATATTTCAAGTATTACGGACTAACACCGGGAAAACTTTTGTTGGCCAGATC
TGATGCGATTGTGATGCACCCTGGTCCGATGAATCGCGGTGTGGAAATTGATTCGGAGGTGGCTGACGGTTCTCAATCGG
TCATTCTACCCCAGGTAGGTTTTGGTATTGCTGTTCGCATGGCAGTCATGTCGATTCTCGCTGGAAATTAA

Upstream 100 bases:

>100_bases
TGTTATCCAGATGACTCGGACTGTTTTGTGCGAAAAGATTTTATTTGTTGATATTTAAAACAAATATGTATTGTTGTAAT
TCTTTCGGAATTCCTGCGTA

Downstream 100 bases:

>100_bases
CGCTCGTATCCTCTATGCATATTTGTATCCAAAATGGCCGTCTGATTGACCCTGACAGCGGTATGGATCGTATTGGCGAT
CTTTATCTGGCTGAAGGCAG

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 316; Mature: 315

Protein sequence:

>316_residues
MSIHPQLNKNGELQHLLTTEGLPVTILRHILDTAESFTGVTERDVKKIPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSA
DVINLSMAVSSQTKGETLLDTVNNLSAMHADMFVVRHNQSGAAHLIARHVGPEIHVINAGDGWHAHPTQALLDMFTIRRY
KQDFHTLRVAIIGDILHSRVARSQIHALTTLGVPEIRVIAPKTLLPARVERLGVHVYHNMTQGLKDVDVLMMLRLQHERM
ESAHLPSTEEYFKYYGLTPGKLLLARSDAIVMHPGPMNRGVEIDSEVADGSQSVILPQVGFGIAVRMAVMSILAGN

Sequences:

>Translated_316_residues
MSIHPQLNKNGELQHLLTTEGLPVTILRHILDTAESFTGVTERDVKKIPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSA
DVINLSMAVSSQTKGETLLDTVNNLSAMHADMFVVRHNQSGAAHLIARHVGPEIHVINAGDGWHAHPTQALLDMFTIRRY
KQDFHTLRVAIIGDILHSRVARSQIHALTTLGVPEIRVIAPKTLLPARVERLGVHVYHNMTQGLKDVDVLMMLRLQHERM
ESAHLPSTEEYFKYYGLTPGKLLLARSDAIVMHPGPMNRGVEIDSEVADGSQSVILPQVGFGIAVRMAVMSILAGN
>Mature_315_residues
SIHPQLNKNGELQHLLTTEGLPVTILRHILDTAESFTGVTERDVKKIPLLRGKSVFNLFFEPSTRTRTTFEIAAKRLSAD
VINLSMAVSSQTKGETLLDTVNNLSAMHADMFVVRHNQSGAAHLIARHVGPEIHVINAGDGWHAHPTQALLDMFTIRRYK
QDFHTLRVAIIGDILHSRVARSQIHALTTLGVPEIRVIAPKTLLPARVERLGVHVYHNMTQGLKDVDVLMMLRLQHERME
SAHLPSTEEYFKYYGLTPGKLLLARSDAIVMHPGPMNRGVEIDSEVADGSQSVILPQVGFGIAVRMAVMSILAGN

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=312, Percent_Identity=34.9358974358974, Blast_Score=163, Evalue=2e-40,
Organism=Escherichia coli, GI2367364, Length=309, Percent_Identity=31.7152103559871, Blast_Score=137, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI193204318, Length=333, Percent_Identity=33.3333333333333, Blast_Score=131, Evalue=5e-31,
Organism=Saccharomyces cerevisiae, GI6322331, Length=277, Percent_Identity=34.2960288808664, Blast_Score=134, Evalue=2e-32,
Organism=Drosophila melanogaster, GI24642586, Length=314, Percent_Identity=33.7579617834395, Blast_Score=139, Evalue=3e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_NITEC (Q0AIU3)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746693.1
- ProteinModelPortal:   Q0AIU3
- SMR:   Q0AIU3
- STRING:   Q0AIU3
- GeneID:   4271915
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0451
- NMPDR:   fig|335283.3.peg.1158
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   NEUT335283:NEUT_0451-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 35038; Mature: 34907

Theoretical pI: Translated: 9.36; Mature: 9.36

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIHPQLNKNGELQHLLTTEGLPVTILRHILDTAESFTGVTERDVKKIPLLRGKSVFNLF
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEE
FEPSTRTRTTFEIAAKRLSADVINLSMAVSSQTKGETLLDTVNNLSAMHADMFVVRHNQS
ECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCC
GAAHLIARHVGPEIHVINAGDGWHAHPTQALLDMFTIRRYKQDFHTLRVAIIGDILHSRV
CHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSQIHALTTLGVPEIRVIAPKTLLPARVERLGVHVYHNMTQGLKDVDVLMMLRLQHERM
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ESAHLPSTEEYFKYYGLTPGKLLLARSDAIVMHPGPMNRGVEIDSEVADGSQSVILPQVG
HHCCCCCHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHCCCCCEEEECCCC
FGIAVRMAVMSILAGN
HHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIHPQLNKNGELQHLLTTEGLPVTILRHILDTAESFTGVTERDVKKIPLLRGKSVFNLF
CCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCEEEEE
FEPSTRTRTTFEIAAKRLSADVINLSMAVSSQTKGETLLDTVNNLSAMHADMFVVRHNQS
ECCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHHHHHEEEEEEECCC
GAAHLIARHVGPEIHVINAGDGWHAHPTQALLDMFTIRRYKQDFHTLRVAIIGDILHSRV
CHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARSQIHALTTLGVPEIRVIAPKTLLPARVERLGVHVYHNMTQGLKDVDVLMMLRLQHERM
HHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ESAHLPSTEEYFKYYGLTPGKLLLARSDAIVMHPGPMNRGVEIDSEVADGSQSVILPQVG
HHCCCCCHHHHHHHCCCCCCEEEEEECCEEEECCCCCCCCCCCCHHHCCCCCEEEECCCC
FGIAVRMAVMSILAGN
HHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA