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The map label for this gene is trmJ [H]

Identifier: 114330429

GI number: 114330429

Start: 415060

End: 415806

Strand: Direct

Name: trmJ [H]

Synonym: Neut_0408

Alternate gene names: 114330429

Gene position: 415060-415806 (Clockwise)

Preceding gene: 114330423

Following gene: 114330431

Centisome position: 15.6

GC content: 54.62

Gene sequence:

>747_bases
ATGAATCCTGCCTCGCCGCTCGATAATGTCCGGATTGTGTTGAGCCATACCAGCCATCCGGGCAATATCGGTGCAACGGC
CCGTGCCATGAAAACCATGGGGCTGAACAAGCTTTACCTGATCAACCCGAAATCCTTTCCGGATAGTGAGGCGGATGCCC
GCGCCTCCAATGCACGCGACATACTTGAAACGGCGCACGTCTGCAGTAACCTGGAGGAGGCGCTGGGGGATACTTTTCTG
GCTGCTGCCCTGACTGCTCGCCGGCGTGATTTGCCGCACCCGACCTGTAATGCCAGGCAGGCAGCCATTGAGCTGATCCG
GCATGCACAGCATCATCCGGTAGCGTTGCTGTTTGGCCGGGAAAGTGCCGGATTGACCGCAGCGGAAATCAGTCATTGCC
AGGTAACGGTGCAGATTCCGGCTAACCCTGATTGTTCTTCGCTTAACCTGGCAGCTGCGGTACAAGTTATGGCTTATGAG
CTGCGCATGGTACTTGAAGAAGATGCTGCTGCGCATTATTTTGCATCCCGATTGCCGGATGCCGCCAGCCTGCATGAAAT
TGAAGGATTACACTATCACCTGGAACGGGTCATGATCCACAGTGGATTTATGGATCCTGACCAACCCAAACACCTCATGC
GCCGGATACGTCGTTTGTTTGCACGTACCCGGCTGGAAAAGGAAGAGGTGAATCTTTTGCGTGGTCTGCTGACTGCGGTC
GATCCGCATAATTCATCCAAAAGCTGA

Upstream 100 bases:

>100_bases
TTGCCGGCCCGGCGAGCTGCCTTAACTGCGATGGTAAGCGTGGGATGCATGGTAAAAAATTTACTTTAGAAACAAATGAC
TGGAAAACCGCTATTCTAAT

Downstream 100 bases:

>100_bases
TTTTCTCCGGGCGCTCAGCCTATTCTGGCTGATGTTCCTAAAGCCACGCTGTTTCCTGGCTCTGTTTTTGGTGATTCGGT
TGATTAATTCGGAGCAACGG

Product: RNA methyltransferase

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MNPASPLDNVRIVLSHTSHPGNIGATARAMKTMGLNKLYLINPKSFPDSEADARASNARDILETAHVCSNLEEALGDTFL
AAALTARRRDLPHPTCNARQAAIELIRHAQHHPVALLFGRESAGLTAAEISHCQVTVQIPANPDCSSLNLAAAVQVMAYE
LRMVLEEDAAAHYFASRLPDAASLHEIEGLHYHLERVMIHSGFMDPDQPKHLMRRIRRLFARTRLEKEEVNLLRGLLTAV
DPHNSSKS

Sequences:

>Translated_248_residues
MNPASPLDNVRIVLSHTSHPGNIGATARAMKTMGLNKLYLINPKSFPDSEADARASNARDILETAHVCSNLEEALGDTFL
AAALTARRRDLPHPTCNARQAAIELIRHAQHHPVALLFGRESAGLTAAEISHCQVTVQIPANPDCSSLNLAAAVQVMAYE
LRMVLEEDAAAHYFASRLPDAASLHEIEGLHYHLERVMIHSGFMDPDQPKHLMRRIRRLFARTRLEKEEVNLLRGLLTAV
DPHNSSKS
>Mature_248_residues
MNPASPLDNVRIVLSHTSHPGNIGATARAMKTMGLNKLYLINPKSFPDSEADARASNARDILETAHVCSNLEEALGDTFL
AAALTARRRDLPHPTCNARQAAIELIRHAQHHPVALLFGRESAGLTAAEISHCQVTVQIPANPDCSSLNLAAAVQVMAYE
LRMVLEEDAAAHYFASRLPDAASLHEIEGLHYHLERVMIHSGFMDPDQPKHLMRRIRRLFARTRLEKEEVNLLRGLLTAV
DPHNSSKS

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=244, Percent_Identity=43.0327868852459, Blast_Score=205, Evalue=2e-54,
Organism=Escherichia coli, GI1790865, Length=165, Percent_Identity=32.7272727272727, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27334; Mature: 27334

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPASPLDNVRIVLSHTSHPGNIGATARAMKTMGLNKLYLINPKSFPDSEADARASNARD
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
ILETAHVCSNLEEALGDTFLAAALTARRRDLPHPTCNARQAAIELIRHAQHHPVALLFGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
ESAGLTAAEISHCQVTVQIPANPDCSSLNLAAAVQVMAYELRMVLEEDAAAHYFASRLPD
CCCCCCHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AASLHEIEGLHYHLERVMIHSGFMDPDQPKHLMRRIRRLFARTRLEKEEVNLLRGLLTAV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
DPHNSSKS
CCCCCCCC
>Mature Secondary Structure
MNPASPLDNVRIVLSHTSHPGNIGATARAMKTMGLNKLYLINPKSFPDSEADARASNARD
CCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHH
ILETAHVCSNLEEALGDTFLAAALTARRRDLPHPTCNARQAAIELIRHAQHHPVALLFGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCEEEEEEC
ESAGLTAAEISHCQVTVQIPANPDCSSLNLAAAVQVMAYELRMVLEEDAAAHYFASRLPD
CCCCCCHHHHCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
AASLHEIEGLHYHLERVMIHSGFMDPDQPKHLMRRIRRLFARTRLEKEEVNLLRGLLTAV
CHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHC
DPHNSSKS
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA