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The map label for this gene is pyrF

Identifier: 114330423

GI number: 114330423

Start: 409502

End: 410197

Strand: Direct

Name: pyrF

Synonym: Neut_0402

Alternate gene names: 114330423

Gene position: 409502-410197 (Clockwise)

Preceding gene: 114330422

Following gene: 114330429

Centisome position: 15.39

GC content: 48.71

Gene sequence:

>696_bases
ATGAATGATTCGCGTATTATCGTCGCACTGGATTTTTCTGACCAAGGCACGGCGTTGAATTTTGCAGCCACGCTGGATAG
CAGACTTTGTCGGATTAAGGTTGGGAAAGAACTTTTTACCCTGGCAGGTCCACAGCTGATCGAGAAATTGATGCGACTGG
GCTTTGATGTTTTTCTTGATTTGAAATTTCACGATATTCCGAACACTGTTGCGGCGGCCTGTAGTGTTGCGTCTAGTTTG
GGTGTATGGATGATCAATGTACATGCGCTGGGAGGAAGCAAAATGTTGCTTGCTGCCCGGCAGGCGTTGGATGGAAAGAG
AACCAGACTCATCGCTGTAACCTTGCTTACCAGTATGAATCATGATGACTTAAGTGAGCTAGGCATATCTGGCTCGCCGG
AAATTATCGTGCAGCGACTGGCTCTGCTTGCACAGCGCTGTGGTTTGGATGGTGTGGTTTGCTCGGCGCTGGAAGCAGCC
AGTCTGAGAGAGAAGGCAGGTAAGGATTTCTGGCTGATAACTCCCGGTATTCGATCATCAAATGATAATAGGGATGATCA
GGCAAGGATTGCGACGCCGACTATGGCAATACGGAACGGGGCTAGCTACCTTGTTATAGGCAGGCCGATTACCCGGTCAC
CAGATCCGCTTAAAGCGCTTCAGCGATTTAATGACGAGATTGTTTCGGCTTTATAA

Upstream 100 bases:

>100_bases
GTAGTCGTGCTTTTCCTCTGTTTCGCAGCAGGTGCCCTATTCGGGTATTTATCCTGTCTCATTAAAAAAATCCGTAGGAA
ACCCTGATATTACTGGCTGT

Downstream 100 bases:

>100_bases
TTCAGTAATTTGTACTTCCGGCAGCTTTCTGGAAGATATTGAAAAAACCTTTCTAAGTGCCCAATCCTGACTGGCCAGAA
GGCTGGTGATTAAAACGGTT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MNDSRIIVALDFSDQGTALNFAATLDSRLCRIKVGKELFTLAGPQLIEKLMRLGFDVFLDLKFHDIPNTVAAACSVASSL
GVWMINVHALGGSKMLLAARQALDGKRTRLIAVTLLTSMNHDDLSELGISGSPEIIVQRLALLAQRCGLDGVVCSALEAA
SLREKAGKDFWLITPGIRSSNDNRDDQARIATPTMAIRNGASYLVIGRPITRSPDPLKALQRFNDEIVSAL

Sequences:

>Translated_231_residues
MNDSRIIVALDFSDQGTALNFAATLDSRLCRIKVGKELFTLAGPQLIEKLMRLGFDVFLDLKFHDIPNTVAAACSVASSL
GVWMINVHALGGSKMLLAARQALDGKRTRLIAVTLLTSMNHDDLSELGISGSPEIIVQRLALLAQRCGLDGVVCSALEAA
SLREKAGKDFWLITPGIRSSNDNRDDQARIATPTMAIRNGASYLVIGRPITRSPDPLKALQRFNDEIVSAL
>Mature_231_residues
MNDSRIIVALDFSDQGTALNFAATLDSRLCRIKVGKELFTLAGPQLIEKLMRLGFDVFLDLKFHDIPNTVAAACSVASSL
GVWMINVHALGGSKMLLAARQALDGKRTRLIAVTLLTSMNHDDLSELGISGSPEIIVQRLALLAQRCGLDGVVCSALEAA
SLREKAGKDFWLITPGIRSSNDNRDDQARIATPTMAIRNGASYLVIGRPITRSPDPLKALQRFNDEIVSAL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=227, Percent_Identity=53.3039647577093, Blast_Score=238, Evalue=2e-64,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_NITEC (Q0AIZ1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746645.1
- ProteinModelPortal:   Q0AIZ1
- SMR:   Q0AIZ1
- STRING:   Q0AIZ1
- GeneID:   4273464
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0402
- NMPDR:   fig|335283.3.peg.1945
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q0AIZ1
- ProtClustDB:   PRK00230
- BioCyc:   NEUT335283:NEUT_0402-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24986; Mature: 24986

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 117-117 BINDING 178-178 BINDING 187-187 BINDING 207-207 BINDING 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDSRIIVALDFSDQGTALNFAATLDSRLCRIKVGKELFTLAGPQLIEKLMRLGFDVFLD
CCCCEEEEEEECCCCCCEEEEEEHHHHHHEEEECCHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAAACSVASSLGVWMINVHALGGSKMLLAARQALDGKRTRLIAVTLLTSMN
EEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEECCCC
HDDLSELGISGSPEIIVQRLALLAQRCGLDGVVCSALEAASLREKAGKDFWLITPGIRSS
CCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
NDNRDDQARIATPTMAIRNGASYLVIGRPITRSPDPLKALQRFNDEIVSAL
CCCCCCCCEECCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNDSRIIVALDFSDQGTALNFAATLDSRLCRIKVGKELFTLAGPQLIEKLMRLGFDVFLD
CCCCEEEEEEECCCCCCEEEEEEHHHHHHEEEECCHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTVAAACSVASSLGVWMINVHALGGSKMLLAARQALDGKRTRLIAVTLLTSMN
EEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHCCCCCCEEEEEEEECCCC
HDDLSELGISGSPEIIVQRLALLAQRCGLDGVVCSALEAASLREKAGKDFWLITPGIRSS
CCCHHHCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCC
NDNRDDQARIATPTMAIRNGASYLVIGRPITRSPDPLKALQRFNDEIVSAL
CCCCCCCCEECCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA