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The map label for this gene is rsmB [H]

Identifier: 114330411

GI number: 114330411

Start: 395727

End: 397007

Strand: Reverse

Name: rsmB [H]

Synonym: Neut_0390

Alternate gene names: 114330411

Gene position: 397007-395727 (Counterclockwise)

Preceding gene: 114330412

Following gene: 114330410

Centisome position: 14.92

GC content: 49.02

Gene sequence:

>1281_bases
ATGATTGAAATTCAATTACTGGCTATACAGGCAATCGGGGAGGTTTTTGCAGGCGCCAATCTGAACGAAGTATTGCGTAA
CATCTGGCAGATCAATTCCCGGCTTACGCCCCAACAACGCGGCGCCATTCAGGACATTGCCTATGGCACGCTCCGCCATT
ATGGTCAGCTTGACGCCATCCTTCATCAATTATTAAATAAACCGGCACAAGACAAATCACTTCATTACCTGTTGGCAATC
GCACTCTACCAGCTTTGTTACAGCAAAGCCCCGGCACATGCCATTGTCGATCACGCCGTGTCATCTTCCCGCAAAATCAC
ACGCAATCCTGCCGCATCGGGATTGATCAACGCTGTGTTACGTAATTTCATGCGGCAGCGCACTTTATTGCTTGATCAAA
TCAAGAGCAATGAAGTCGCCCGCTATTCCTATCCGCAATGGTGGATTGACAAACTGCGTCAGCAATATCCGCAGGACTAT
AAAGCCATTCTGCTCGCAGGCAATGAGCACCCTGCTATGATTTTACGCATTAATTCGTTACGCACCTCGGTTAATCATTA
TCAGGCAATGCTGGATGCTCAGGGGATTGGCTCCGAATGGTTGGGGGGCAGCGCCCTGCAACTTATCAGGCCGGTCTCTG
TGGAGAAATTACCCGGGTTTTCCGAAGGACTGGTGACTGTACAGGACGCGGGCGCACAATTTGCCGCCCCCCTGCTGGAT
GTCAAAGCAGGGATGCGTGTGCTGGATGCCTGTGCCGCACCAGGCGGAAAAAGCACCCATCTGGCTGAGTTAACTGAAAA
TATCAAATTGACGGTGCTGGATAAATATGAAGATCGGCTTACCAGGTTGACAGAAAATTTCTCTCGCCTGCAAATAACTG
ATTATCAGTTGATTTGTGGCGATGCAATGAATCCGTCGCAATGGTGGAATGGCCAATTGTATGATCGAATCCTGGCTGAT
GTCCCGTGTTCTGCTTCAGGCGTGGTCAACCGCCACCCGGATATCAAATGGCTCAGAAGACCAGGTGACACAGATAACTT
CGCACAAATTCAGACAACCATATTGAATGAACTGTGGCCATTACTGCGGCATGGAGGAAAACTGCTGTATGCCACCTGCT
CCGTATTCAATGAAGAAAATAACCTGATTACTGAAAAATTCCTGTCAATCCATCAGGATGCCAGCAGATTACCCTGCTCC
CACGAAGAAATTAACAACGGCCAGCTGCTGCCGGGACCGCACCATGATGGCTTTTTTTATGCCTTGCTCCAAAAAAACTG
A

Upstream 100 bases:

>100_bases
AAAAAAACTGACTGCCGCGCAATTCCTTGCCGGGCACCCTTTGCAACCTGGAGAACATTTCCAAAAAACCACACAGGGTC
ACCTGTGATTTTTCTTGAAA

Downstream 100 bases:

>100_bases
CATGCACGCTGACATAAGGAGTCCCTGGCTCAGTATCGTTTTATTGTTAATCGTGCTGTTTTCATCAACCACACTTGCCG
CCAAAGGACATATAGAAATA

Product: sun protein

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 426; Mature: 426

Protein sequence:

>426_residues
MIEIQLLAIQAIGEVFAGANLNEVLRNIWQINSRLTPQQRGAIQDIAYGTLRHYGQLDAILHQLLNKPAQDKSLHYLLAI
ALYQLCYSKAPAHAIVDHAVSSSRKITRNPAASGLINAVLRNFMRQRTLLLDQIKSNEVARYSYPQWWIDKLRQQYPQDY
KAILLAGNEHPAMILRINSLRTSVNHYQAMLDAQGIGSEWLGGSALQLIRPVSVEKLPGFSEGLVTVQDAGAQFAAPLLD
VKAGMRVLDACAAPGGKSTHLAELTENIKLTVLDKYEDRLTRLTENFSRLQITDYQLICGDAMNPSQWWNGQLYDRILAD
VPCSASGVVNRHPDIKWLRRPGDTDNFAQIQTTILNELWPLLRHGGKLLYATCSVFNEENNLITEKFLSIHQDASRLPCS
HEEINNGQLLPGPHHDGFFYALLQKN

Sequences:

>Translated_426_residues
MIEIQLLAIQAIGEVFAGANLNEVLRNIWQINSRLTPQQRGAIQDIAYGTLRHYGQLDAILHQLLNKPAQDKSLHYLLAI
ALYQLCYSKAPAHAIVDHAVSSSRKITRNPAASGLINAVLRNFMRQRTLLLDQIKSNEVARYSYPQWWIDKLRQQYPQDY
KAILLAGNEHPAMILRINSLRTSVNHYQAMLDAQGIGSEWLGGSALQLIRPVSVEKLPGFSEGLVTVQDAGAQFAAPLLD
VKAGMRVLDACAAPGGKSTHLAELTENIKLTVLDKYEDRLTRLTENFSRLQITDYQLICGDAMNPSQWWNGQLYDRILAD
VPCSASGVVNRHPDIKWLRRPGDTDNFAQIQTTILNELWPLLRHGGKLLYATCSVFNEENNLITEKFLSIHQDASRLPCS
HEEINNGQLLPGPHHDGFFYALLQKN
>Mature_426_residues
MIEIQLLAIQAIGEVFAGANLNEVLRNIWQINSRLTPQQRGAIQDIAYGTLRHYGQLDAILHQLLNKPAQDKSLHYLLAI
ALYQLCYSKAPAHAIVDHAVSSSRKITRNPAASGLINAVLRNFMRQRTLLLDQIKSNEVARYSYPQWWIDKLRQQYPQDY
KAILLAGNEHPAMILRINSLRTSVNHYQAMLDAQGIGSEWLGGSALQLIRPVSVEKLPGFSEGLVTVQDAGAQFAAPLLD
VKAGMRVLDACAAPGGKSTHLAELTENIKLTVLDKYEDRLTRLTENFSRLQITDYQLICGDAMNPSQWWNGQLYDRILAD
VPCSASGVVNRHPDIKWLRRPGDTDNFAQIQTTILNELWPLLRHGGKLLYATCSVFNEENNLITEKFLSIHQDASRLPCS
HEEINNGQLLPGPHHDGFFYALLQKN

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=286, Percent_Identity=28.3216783216783, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI76150623, Length=286, Percent_Identity=28.3216783216783, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI32698918, Length=200, Percent_Identity=25.5, Blast_Score=72, Evalue=9e-13,
Organism=Homo sapiens, GI8922322, Length=297, Percent_Identity=27.9461279461279, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI23199998, Length=297, Percent_Identity=27.9461279461279, Blast_Score=70, Evalue=4e-12,
Organism=Homo sapiens, GI270288818, Length=297, Percent_Identity=27.9461279461279, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI270288816, Length=297, Percent_Identity=27.9461279461279, Blast_Score=70, Evalue=5e-12,
Organism=Escherichia coli, GI2367212, Length=426, Percent_Identity=40.3755868544601, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87081985, Length=214, Percent_Identity=29.9065420560748, Blast_Score=83, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17536757, Length=274, Percent_Identity=25.9124087591241, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI71998419, Length=173, Percent_Identity=28.3236994219653, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI115534021, Length=173, Percent_Identity=28.3236994219653, Blast_Score=69, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324268, Length=287, Percent_Identity=26.4808362369338, Blast_Score=86, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6319447, Length=207, Percent_Identity=28.9855072463768, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI22024126, Length=273, Percent_Identity=27.4725274725275, Blast_Score=96, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19920494, Length=157, Percent_Identity=35.6687898089172, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 47828; Mature: 47828

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEIQLLAIQAIGEVFAGANLNEVLRNIWQINSRLTPQQRGAIQDIAYGTLRHYGQLDAI
CEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
LHQLLNKPAQDKSLHYLLAIALYQLCYSKAPAHAIVDHAVSSSRKITRNPAASGLINAVL
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
RNFMRQRTLLLDQIKSNEVARYSYPQWWIDKLRQQYPQDYKAILLAGNEHPAMILRINSL
HHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEHHH
RTSVNHYQAMLDAQGIGSEWLGGSALQLIRPVSVEKLPGFSEGLVTVQDAGAQFAAPLLD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCEEEEECCCCHHHHHHHH
VKAGMRVLDACAAPGGKSTHLAELTENIKLTVLDKYEDRLTRLTENFSRLQITDYQLICG
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHCHHHEEEECEEEEEC
DAMNPSQWWNGQLYDRILADVPCSASGVVNRHPDIKWLRRPGDTDNFAQIQTTILNELWP
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
LLRHGGKLLYATCSVFNEENNLITEKFLSIHQDASRLPCSHEEINNGQLLPGPHHDGFFY
HHHCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEE
ALLQKN
EEEECC
>Mature Secondary Structure
MIEIQLLAIQAIGEVFAGANLNEVLRNIWQINSRLTPQQRGAIQDIAYGTLRHYGQLDAI
CEEEEEEHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHH
LHQLLNKPAQDKSLHYLLAIALYQLCYSKAPAHAIVDHAVSSSRKITRNPAASGLINAVL
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
RNFMRQRTLLLDQIKSNEVARYSYPQWWIDKLRQQYPQDYKAILLAGNEHPAMILRINSL
HHHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEHHH
RTSVNHYQAMLDAQGIGSEWLGGSALQLIRPVSVEKLPGFSEGLVTVQDAGAQFAAPLLD
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHHHCCCCCCCEEEEECCCCHHHHHHHH
VKAGMRVLDACAAPGGKSTHLAELTENIKLTVLDKYEDRLTRLTENFSRLQITDYQLICG
HHHHHHHHHHHCCCCCCCCHHHHHHCCCEEEEECHHHHHHHHHHHCHHHEEEECEEEEEC
DAMNPSQWWNGQLYDRILADVPCSASGVVNRHPDIKWLRRPGDTDNFAQIQTTILNELWP
CCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHH
LLRHGGKLLYATCSVFNEENNLITEKFLSIHQDASRLPCSHEEINNGQLLPGPHHDGFFY
HHHCCCEEEEEEEHHHCCCCCHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCEEE
ALLQKN
EEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]