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The map label for this gene is flgJ [H]

Identifier: 114330366

GI number: 114330366

Start: 348397

End: 349404

Strand: Direct

Name: flgJ [H]

Synonym: Neut_0343

Alternate gene names: 114330366

Gene position: 348397-349404 (Clockwise)

Preceding gene: 114330365

Following gene: 114330367

Centisome position: 13.09

GC content: 52.08

Gene sequence:

>1008_bases
ATGATCAATTCTCCCGACATTTCCGGCCAGTTGGCCATCGATGCGCAAGCAGTTGATCAATTGCGCAATCAAGCCCGGCG
CGATCCGCAGGAAGGGCTGAAGCAGACGGCAAAGCAGTTTGAGGCGCTGTTTCTCAACATGATGATGAAAAGTATGCGTG
AAGCCACGCCCAAAGAAGGATTGTTTGATAGTGATCAATCGCGTTTTTATACCCAGATGCTTGATCAGCAACTGGTGCAG
AATCTTTCTGACAAGGGAATTGGCCTGGCAGACATGATCGTACGGCAATTGTCACAAGCTGTCGGCAAGGAACAGGCTAA
CGCCATAGACGGAGCAGAATCTCTCTTGACAGCTATTGTCGGAAATGATGAGAAACCACTGGTTTCAGTGGCGCCGGGGC
AAACAAAGGATCTGCCTTCCCAACTCTGGACGAATCTTAATCGGGCTACCGCTAAACCAAATTTTTCAGCTGTCTCGCCC
GCATGGCGAACAAACCGGATGGGAATTGCTTCCAGCTCCGGGAATGATGGTCCTGAGATAGCTGCGGTTGATCAGCCCAG
AGAGTTTGTGCTGGATGTATTGCCGCATGCCCGCAAAGTGGCGCGTGAAACCGGCATTCCTGAACATTTCATGATTGCCC
AGGCCGCGCTGGAAACTGGCTGGGGCAGGCATCAGATCCGGCGGACGGACAATCAGCCAAGCTTCAATCTGTTTGGCATC
AAGGCAAGCGGGAACTGGCGTGGACAAGTGGTGGAAACCATGACCACCGAATATGTGGATGGTACACCGCAAAAGATCAG
GGAAAAATTCCGGGCATACAATTCCTACGAGGAAGCCTTCCGGGATTATGCGCGGTTACTGCAGAACACTCCCCGTTATG
CAGCCGTGCTGAAAAGCCGCAGTGCCACAGCGTTTGCCTGGGGGTTGCAACAGGCTGGTTATGCGACTGATCCATCCTAC
GCGGAAAAGCTGCTGAAGATCATCAACAGTGATGCGTTGAACATATAA

Upstream 100 bases:

>100_bases
ATTGGTGCCACTCCGCAGGATTTGCTGGCAATTTTGCAAGCACTCAAGGCAGCGGGATCATTGCGGGCAGAGCTGGAAAT
TATTTGATTAGGTTGGCGAT

Downstream 100 bases:

>100_bases
GGAGTTGTTAAATATTTGCTGCTTTTTTGCGAATACCAGCATCCAGTAGCAGTGGTTCCAAAGGTGTTGGATTGCTTCAC
GCCACCCACCTGTTTCACTA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MINSPDISGQLAIDAQAVDQLRNQARRDPQEGLKQTAKQFEALFLNMMMKSMREATPKEGLFDSDQSRFYTQMLDQQLVQ
NLSDKGIGLADMIVRQLSQAVGKEQANAIDGAESLLTAIVGNDEKPLVSVAPGQTKDLPSQLWTNLNRATAKPNFSAVSP
AWRTNRMGIASSSGNDGPEIAAVDQPREFVLDVLPHARKVARETGIPEHFMIAQAALETGWGRHQIRRTDNQPSFNLFGI
KASGNWRGQVVETMTTEYVDGTPQKIREKFRAYNSYEEAFRDYARLLQNTPRYAAVLKSRSATAFAWGLQQAGYATDPSY
AEKLLKIINSDALNI

Sequences:

>Translated_335_residues
MINSPDISGQLAIDAQAVDQLRNQARRDPQEGLKQTAKQFEALFLNMMMKSMREATPKEGLFDSDQSRFYTQMLDQQLVQ
NLSDKGIGLADMIVRQLSQAVGKEQANAIDGAESLLTAIVGNDEKPLVSVAPGQTKDLPSQLWTNLNRATAKPNFSAVSP
AWRTNRMGIASSSGNDGPEIAAVDQPREFVLDVLPHARKVARETGIPEHFMIAQAALETGWGRHQIRRTDNQPSFNLFGI
KASGNWRGQVVETMTTEYVDGTPQKIREKFRAYNSYEEAFRDYARLLQNTPRYAAVLKSRSATAFAWGLQQAGYATDPSY
AEKLLKIINSDALNI
>Mature_335_residues
MINSPDISGQLAIDAQAVDQLRNQARRDPQEGLKQTAKQFEALFLNMMMKSMREATPKEGLFDSDQSRFYTQMLDQQLVQ
NLSDKGIGLADMIVRQLSQAVGKEQANAIDGAESLLTAIVGNDEKPLVSVAPGQTKDLPSQLWTNLNRATAKPNFSAVSP
AWRTNRMGIASSSGNDGPEIAAVDQPREFVLDVLPHARKVARETGIPEHFMIAQAALETGWGRHQIRRTDNQPSFNLFGI
KASGNWRGQVVETMTTEYVDGTPQKIREKFRAYNSYEEAFRDYARLLQNTPRYAAVLKSRSATAFAWGLQQAGYATDPSY
AEKLLKIINSDALNI

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=331, Percent_Identity=40.1812688821752, Blast_Score=229, Evalue=3e-61,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 37180; Mature: 37180

Theoretical pI: Translated: 7.74; Mature: 7.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSPDISGQLAIDAQAVDQLRNQARRDPQEGLKQTAKQFEALFLNMMMKSMREATPKEG
CCCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LFDSDQSRFYTQMLDQQLVQNLSDKGIGLADMIVRQLSQAVGKEQANAIDGAESLLTAIV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
GNDEKPLVSVAPGQTKDLPSQLWTNLNRATAKPNFSAVSPAWRTNRMGIASSSGNDGPEI
CCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCE
AAVDQPREFVLDVLPHARKVARETGIPEHFMIAQAALETGWGRHQIRRTDNQPSFNLFGI
EECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEE
KASGNWRGQVVETMTTEYVDGTPQKIREKFRAYNSYEEAFRDYARLLQNTPRYAAVLKSR
EECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHCC
SATAFAWGLQQAGYATDPSYAEKLLKIINSDALNI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MINSPDISGQLAIDAQAVDQLRNQARRDPQEGLKQTAKQFEALFLNMMMKSMREATPKEG
CCCCCCCCCEEEECHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
LFDSDQSRFYTQMLDQQLVQNLSDKGIGLADMIVRQLSQAVGKEQANAIDGAESLLTAIV
CCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
GNDEKPLVSVAPGQTKDLPSQLWTNLNRATAKPNFSAVSPAWRTNRMGIASSSGNDGPEI
CCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCE
AAVDQPREFVLDVLPHARKVARETGIPEHFMIAQAALETGWGRHQIRRTDNQPSFNLFGI
EECCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHCCCCCCCEEEEEE
KASGNWRGQVVETMTTEYVDGTPQKIREKFRAYNSYEEAFRDYARLLQNTPRYAAVLKSR
EECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCHHHHHHHCC
SATAFAWGLQQAGYATDPSYAEKLLKIINSDALNI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]