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The map label for this gene is insF1 [C]

Identifier: 114330330

GI number: 114330330

Start: 311300

End: 312127

Strand: Reverse

Name: insF1 [C]

Synonym: Neut_0303

Alternate gene names: 114330330

Gene position: 312127-311300 (Counterclockwise)

Preceding gene: 114330331

Following gene: 114330328

Centisome position: 11.73

GC content: 48.43

Gene sequence:

>828_bases
ATGTGCCGGCTATTGGGTGTCAGTCGCAGTGCTTACTATGACTATGAACAACGCCGCTGCAATTGCCCGGATGATCTACA
CCACAGGCAACTACTTAATGCTGTGCAGAATATTGCAAAATCATGCGATTACACCTATGGCAGTCGCAGGATGAAACGAG
CGCTAAATGCCTTGGACTATCGGGTTAGTCGCTGGAAGGCGAGAAGACTAATGCAAGAAGCCGGGATACAAGTGAAACAC
CGGAAGAAGTACAAGGTGACGACGGACAGCAATCACCCGTTGCCAGTGTTTGAGAACCAATTGAATCGGCAATTTACGGT
TGCCAGACCGGATCAAGTCTATGTTTGCGACATTACCTGCATTTGGACTCAGGAAGGCTGGCTGTATCTGGCGGTTGTCA
TCGATTTGTTCTCCCGAAAGGTGGTGGGCTGGAGTATGAGCTCACGGATGAAAGCAACACTGGTTTGTGATGCATTACGA
ATGGCGATCTGGCTACACCGGCCACCGCCTGGCCTGATCGTGCATTCGGATCGTGGGTCGCAGTATGCTAGCAAAGCATA
CCGCAATCTACTGAAAGCATACGGTTTTATTGGCAGTATGAGTCGTCTGGGAAATTGTTGGGATAATGCGGTTGCGGAAA
GTTTCTTTGGCAGCCTCAAGCAGGAACGCTGCCAATGGCGGCATTACCAAACCCGCCATGCAGCACAGCAGGATATTTTG
CAGTATATCGCCGTGTTTTATAATAACCAAAGACTGCATTCATACCTGGATTACAAAAGTCCGAATCAATATGAAGCAGA
AGCAGCAAAATCGATAAAAGCAGCTTAA

Upstream 100 bases:

>100_bases
AGCGGCAAATACTAAAGGAGGCGGCGGTCTTTTTCGCGAAAGAAACGAAGTGAAGTATTCGTTCATCACCCAAAAGAAAA
AGACCTACCCGGTCGGCCTG

Downstream 100 bases:

>100_bases
CTGAGGTGTCCGATTTTACTTGACCACGTTAATCCGGTTTCCAGAAGGGAGGGATCGTCCTTATGGCTGGAGTGTCACCG
GTAATGTAAAAATGCCCCCC

Product: integrase catalytic subunit

Products: NA

Alternate protein names: ISO-S3 31 kDa protein [H]

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDYRVSRWKARRLMQEAGIQVKH
RKKYKVTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITCIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKATLVCDALR
MAIWLHRPPPGLIVHSDRGSQYASKAYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDIL
QYIAVFYNNQRLHSYLDYKSPNQYEAEAAKSIKAA

Sequences:

>Translated_275_residues
MCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDYRVSRWKARRLMQEAGIQVKH
RKKYKVTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITCIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKATLVCDALR
MAIWLHRPPPGLIVHSDRGSQYASKAYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDIL
QYIAVFYNNQRLHSYLDYKSPNQYEAEAAKSIKAA
>Mature_275_residues
MCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDYRVSRWKARRLMQEAGIQVKH
RKKYKVTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITCIWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKATLVCDALR
MAIWLHRPPPGLIVHSDRGSQYASKAYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDIL
QYIAVFYNNQRLHSYLDYKSPNQYEAEAAKSIKAA

Specific function: Involved in the transposition of the insertion sequence is3 [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Homo sapiens, GI310129419, Length=228, Percent_Identity=37.280701754386, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1788405, Length=266, Percent_Identity=42.1052631578947, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787263, Length=266, Percent_Identity=42.1052631578947, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786753, Length=266, Percent_Identity=42.1052631578947, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786570, Length=266, Percent_Identity=42.1052631578947, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1786490, Length=266, Percent_Identity=42.1052631578947, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1789981, Length=268, Percent_Identity=31.3432835820896, Blast_Score=122, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 32165; Mature: 32165

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDY
CCCEEECCHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RVSRWKARRLMQEAGIQVKHRKKYKVTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITC
HHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCHHHHHCCCEEEEECCCEEEEEEEEE
IWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKATLVCDALRMAIWLHRPPPGLIVHSDRGS
EEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
QYASKAYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYIAVFYNNQRLHSYLDYKSPNQYEAEAAKSIKAA
HHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MCRLLGVSRSAYYDYEQRRCNCPDDLHHRQLLNAVQNIAKSCDYTYGSRRMKRALNALDY
CCCEEECCHHHHHCHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
RVSRWKARRLMQEAGIQVKHRKKYKVTTDSNHPLPVFENQLNRQFTVARPDQVYVCDITC
HHHHHHHHHHHHHCCCEEEECCEEEEECCCCCCCCHHHHHCCCEEEEECCCEEEEEEEEE
IWTQEGWLYLAVVIDLFSRKVVGWSMSSRMKATLVCDALRMAIWLHRPPPGLIVHSDRGS
EEECCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCC
QYASKAYRNLLKAYGFIGSMSRLGNCWDNAVAESFFGSLKQERCQWRHYQTRHAAQQDIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QYIAVFYNNQRLHSYLDYKSPNQYEAEAAKSIKAA
HHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2824781 [H]