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The map label for this gene is murD

Identifier: 114330275

GI number: 114330275

Start: 261326

End: 262738

Strand: Reverse

Name: murD

Synonym: Neut_0248

Alternate gene names: 114330275

Gene position: 262738-261326 (Counterclockwise)

Preceding gene: 114330276

Following gene: 114330274

Centisome position: 9.87

GC content: 51.17

Gene sequence:

>1413_bases
ATGAATTACGCCGACAAAAAAATATTGGTTCTGGGTATGGGTAAAACCGGCATCTCCATGGTCAAGTGGCTTTCCCGTGC
CGGAGCTCGGGTGTCCGTAGCCGATACACGTACCACCCCGCCGAATCTTGAGCTGCTGAGTCAGATAGTGCCGCTTGAAA
CGATTTTCTGTGGTCCATTCAAGGCAGAGCTGTTCAAGGACATTGATGCAATTGCTATCAGCCCCGGCGTGGCAATTGCC
GAGCCACTGGTGCAGGCTGCATTGCAACAGGGCGTATCAGTAATAGGAGATATCGAGCTGTTCGCCATTGCACTTGATCA
GCACGCGCCACCGGGTACGAAAATTCTGGCAATTACCGGATCTAATGGGAAAACCACTGTTGCCACCATGGTAGGTGAAA
TGGCAAGGAATACCGGATGGGATGTTGAAGTAGCGGGTAACATCGGCCTGGCGGCGCTGGATGCGCTGATGCAGCGAATA
GATACAGGAAAATGGCCGCATTTATGGGTACTTGAGCTGTCAAGTTTTCAGCTAGAAACCACCAGCAGTTTACGTCCCAA
TGCTGCCGTCGTATTAAACCTCAGCGAAGATCATCTGGACCGCTACCGCATTATCGAGGAATACGCAGCCGCAAAAGCAA
GGATATTTCCCGATCCACATAACAGTTGTGTGCAGGTACTCAACCGGGAAGATGCCCGGGTATACGCCATGGCTCACGAA
AACAGCAAACAATTAACTTTTGGTTTGTCCGCACCGGCATTCGACGATGAATTCGGTGTGTTACCCAGTGGATCAGATTT
GTGGTTGGCACAGGGCACCACTCGCTTGATGAAAGTCAGCGAACTTGCTGTCGCGGGATTGCATAACGCGGCCAACGCAC
TGGCGGCACTGGCATTATGTCGGGCCATTGATCTACCTTTTGAACCGTTGTTGCATGCTTTGCACATCTTCAAGGGATTG
CCGCATCGCATGCAGAAGATTGCCGAGTTCAATGGCGTGACTTTTTATGATGATTCCAAAAGCACCAACGTGGGCTCCGC
CGTTGCCGCACTGAATGGTTTCCGAAAAAACGTGATTCTGATTGCAGGCGGAGATGGCAAAGGGCAGGATTTCTCACCTC
TGGAACAGCCTGTCAGCAAACATACACGCGGTGTTGTACTGCTGGGAAGAGATGCCGAGAAAATTTCACAGGCAATTCAG
GGCTGCAATGTACCGGTACATCGCGTGGCAACTATGGATGAGGCTGTCCGGGTAAGTTTTTTGCTGGCAGAGCGTGGCGA
TAGCGTTCTACTCTCCCCGGCCTGTGCCAGTCTTGATATGTTCAACAACTACATCCATCGCGCTGAGGTTTTTACGACCG
CTGTCCAGGGAATCGAACACAAATTTATACTCACGGCACAGACATGTCACTAA

Upstream 100 bases:

>100_bases
ATTACGAGCTCAAGGGATGGAAGGAAAACCAGGTTGTAGTGAGATTCTGGATTATTACCATCATTCTGGTGCTGATCGGC
TTATCAACACTGAAACTACG

Downstream 100 bases:

>100_bases
CCGTATGATAAGTCCATCCACTACACATAATCAGCCAATCCAGCCCGAACTAGATCTGCATCTGCTTTCAGCTGTTCTGC
TGTTGCTGGGCCTAGGACTG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MNYADKKILVLGMGKTGISMVKWLSRAGARVSVADTRTTPPNLELLSQIVPLETIFCGPFKAELFKDIDAIAISPGVAIA
EPLVQAALQQGVSVIGDIELFAIALDQHAPPGTKILAITGSNGKTTVATMVGEMARNTGWDVEVAGNIGLAALDALMQRI
DTGKWPHLWVLELSSFQLETTSSLRPNAAVVLNLSEDHLDRYRIIEEYAAAKARIFPDPHNSCVQVLNREDARVYAMAHE
NSKQLTFGLSAPAFDDEFGVLPSGSDLWLAQGTTRLMKVSELAVAGLHNAANALAALALCRAIDLPFEPLLHALHIFKGL
PHRMQKIAEFNGVTFYDDSKSTNVGSAVAALNGFRKNVILIAGGDGKGQDFSPLEQPVSKHTRGVVLLGRDAEKISQAIQ
GCNVPVHRVATMDEAVRVSFLLAERGDSVLLSPACASLDMFNNYIHRAEVFTTAVQGIEHKFILTAQTCH

Sequences:

>Translated_470_residues
MNYADKKILVLGMGKTGISMVKWLSRAGARVSVADTRTTPPNLELLSQIVPLETIFCGPFKAELFKDIDAIAISPGVAIA
EPLVQAALQQGVSVIGDIELFAIALDQHAPPGTKILAITGSNGKTTVATMVGEMARNTGWDVEVAGNIGLAALDALMQRI
DTGKWPHLWVLELSSFQLETTSSLRPNAAVVLNLSEDHLDRYRIIEEYAAAKARIFPDPHNSCVQVLNREDARVYAMAHE
NSKQLTFGLSAPAFDDEFGVLPSGSDLWLAQGTTRLMKVSELAVAGLHNAANALAALALCRAIDLPFEPLLHALHIFKGL
PHRMQKIAEFNGVTFYDDSKSTNVGSAVAALNGFRKNVILIAGGDGKGQDFSPLEQPVSKHTRGVVLLGRDAEKISQAIQ
GCNVPVHRVATMDEAVRVSFLLAERGDSVLLSPACASLDMFNNYIHRAEVFTTAVQGIEHKFILTAQTCH
>Mature_470_residues
MNYADKKILVLGMGKTGISMVKWLSRAGARVSVADTRTTPPNLELLSQIVPLETIFCGPFKAELFKDIDAIAISPGVAIA
EPLVQAALQQGVSVIGDIELFAIALDQHAPPGTKILAITGSNGKTTVATMVGEMARNTGWDVEVAGNIGLAALDALMQRI
DTGKWPHLWVLELSSFQLETTSSLRPNAAVVLNLSEDHLDRYRIIEEYAAAKARIFPDPHNSCVQVLNREDARVYAMAHE
NSKQLTFGLSAPAFDDEFGVLPSGSDLWLAQGTTRLMKVSELAVAGLHNAANALAALALCRAIDLPFEPLLHALHIFKGL
PHRMQKIAEFNGVTFYDDSKSTNVGSAVAALNGFRKNVILIAGGDGKGQDFSPLEQPVSKHTRGVVLLGRDAEKISQAIQ
GCNVPVHRVATMDEAVRVSFLLAERGDSVLLSPACASLDMFNNYIHRAEVFTTAVQGIEHKFILTAQTCH

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA)

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family

Homologues:

Organism=Escherichia coli, GI1786276, Length=453, Percent_Identity=41.5011037527594, Blast_Score=286, Evalue=2e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MURD_NITEC (Q0AJD9)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746497.1
- ProteinModelPortal:   Q0AJD9
- STRING:   Q0AJD9
- GeneID:   4272264
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0248
- NMPDR:   fig|335283.3.peg.2121
- eggNOG:   COG0771
- HOGENOM:   HBG750024
- OMA:   VKYYNDS
- PhylomeDB:   Q0AJD9
- ProtClustDB:   PRK02006
- BioCyc:   NEUT335283:NEUT_0248-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00639
- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762
- Gene3D:   G3DSA:3.90.190.20
- Gene3D:   G3DSA:3.40.1190.10
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR01087

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M; SSF53244 Mur_ligase_C; SSF53623 Mur_ligase_cen

EC number: =6.3.2.9

Molecular weight: Translated: 50661; Mature: 50661

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYADKKILVLGMGKTGISMVKWLSRAGARVSVADTRTTPPNLELLSQIVPLETIFCGPF
CCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHEECCCH
KAELFKDIDAIAISPGVAIAEPLVQAALQQGVSVIGDIELFAIALDQHAPPGTKILAITG
HHHHHHCCHHEEECCCHHHHHHHHHHHHHCCHHHHCCCEEEEEEECCCCCCCCEEEEEEC
SNGKTTVATMVGEMARNTGWDVEVAGNIGLAALDALMQRIDTGKWPHLWVLELSSFQLET
CCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEC
TSSLRPNAAVVLNLSEDHLDRYRIIEEYAAAKARIFPDPHNSCVQVLNREDARVYAMAHE
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHCCCCCEEEEEEEC
NSKQLTFGLSAPAFDDEFGVLPSGSDLWLAQGTTRLMKVSELAVAGLHNAANALAALALC
CCCEEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIDLPFEPLLHALHIFKGLPHRMQKIAEFNGVTFYDDSKSTNVGSAVAALNGFRKNVIL
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCEEE
IAGGDGKGQDFSPLEQPVSKHTRGVVLLGRDAEKISQAIQGCNVPVHRVATMDEAVRVSF
EECCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHEEE
LLAERGDSVLLSPACASLDMFNNYIHRAEVFTTAVQGIEHKFILTAQTCH
EEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC
>Mature Secondary Structure
MNYADKKILVLGMGKTGISMVKWLSRAGARVSVADTRTTPPNLELLSQIVPLETIFCGPF
CCCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCHHHEECCCH
KAELFKDIDAIAISPGVAIAEPLVQAALQQGVSVIGDIELFAIALDQHAPPGTKILAITG
HHHHHHCCHHEEECCCHHHHHHHHHHHHHCCHHHHCCCEEEEEEECCCCCCCCEEEEEEC
SNGKTTVATMVGEMARNTGWDVEVAGNIGLAALDALMQRIDTGKWPHLWVLELSSFQLET
CCCCCHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHHHCCCCCCEEEEEEECCEEEEC
TSSLRPNAAVVLNLSEDHLDRYRIIEEYAAAKARIFPDPHNSCVQVLNREDARVYAMAHE
CCCCCCCEEEEEECCHHHHHHHHHHHHHHHHCEEECCCCCHHHHHHHCCCCCEEEEEEEC
NSKQLTFGLSAPAFDDEFGVLPSGSDLWLAQGTTRLMKVSELAVAGLHNAANALAALALC
CCCEEEEECCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RAIDLPFEPLLHALHIFKGLPHRMQKIAEFNGVTFYDDSKSTNVGSAVAALNGFRKNVIL
HHHCCCHHHHHHHHHHHHCCHHHHHHHHHHCCEEEECCCCCCCHHHHHHHHHCCCCCEEE
IAGGDGKGQDFSPLEQPVSKHTRGVVLLGRDAEKISQAIQGCNVPVHRVATMDEAVRVSF
EECCCCCCCCCCHHHHHHHHCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHEEE
LLAERGDSVLLSPACASLDMFNNYIHRAEVFTTAVQGIEHKFILTAQTCH
EEECCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA