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The map label for this gene is ddl

Identifier: 114330270

GI number: 114330270

Start: 255683

End: 256603

Strand: Reverse

Name: ddl

Synonym: Neut_0243

Alternate gene names: 114330270

Gene position: 256603-255683 (Counterclockwise)

Preceding gene: 114330271

Following gene: 114330269

Centisome position: 9.64

GC content: 48.64

Gene sequence:

>921_bases
ATGAACATTCGTGACCTTGGGAAAGTAGCTGTATTGCTGGGTGGACGTTCCGCTGAACGTGAAATCTCACTTAAAAGCGG
CCATGCCGTACTGGCAGCATTACAGCGAAGCCAGGTTGATGCGCATGCATTTGATCCGGTCGGCCAACCTCTGGAAGATC
TTTTAAAACAGGGCTTTGATCGGGCATTTATTGCGCTGCATGGTCGTTATGGTGAAGATGGCTCTGTTCAGGGGGCACTG
GAGCTGATGGATCTGCCTTATACCGGCAGCGGCATACTCGCCAGCGCACTAGCAATGGATAAATGGCGCACAAAAATGAT
CTGGCAGGCGGCAGGCATCAGCACACCTGACTACGTCATGCTAGATGCTGACAGCAATTTCCAGGAAGTGACTGACAGGC
TGGGATTACCGCTCATCATAAAACCTGCCCGGGAAGGCTCAACCATCGGATTGAACAAAGTGGATTACGCGCAAGATATG
CAATCCGCCTATCAGACTGCCGCACAACACGATTCGCTTGTAATAGCAGAGCAGTTTATTCAGGGAATCGAGCTGACAGC
AGCCATTCTGGATGATGTGCCTCTTCCGCTGGTACGAATCGATGTGACAGAAGGTCTATATGATTATCAGGCTAAATATT
TTTCTGAAAGCACACGCTACACTTGCCCAAGCGGTTTGTCGAAAATATTGACAACCCGCATACAGGAACAGGCTTTATAT
GCTCATCGGATACTGGGATGTACCGGCTGGAGCAGAGTGGATCTGATTCTGGATAAAAATGGCCAGCCTTTTTTCCTTGA
GGCCAATACTTCGCCAGGAATGACTAATCACAGTCTTGTACCGATGGCGGCACAGGCTGCAGGTATTTCCTTTGATGAAC
TTGTTATACAAATTCTGGAGTTGAGTTGTGAGTATGCTTAA

Upstream 100 bases:

>100_bases
AGACAGTGATTAATACTGTGCAAGATATCGTTTACAAGAAAACCGAGATCAGGCTGGTCACGGAAGTACGCATTATCGGA
CAGCACAAAGGAAACGAGCT

Downstream 100 bases:

>100_bases
AGATAGAAATCCAGATCAAGGTGATCAGGGCAAATTGATACTGTAACCAGGACATATGTGGAACGATCATCAATCCTTAA
ATCTTCTAGCAAACATACTG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MNIRDLGKVAVLLGGRSAEREISLKSGHAVLAALQRSQVDAHAFDPVGQPLEDLLKQGFDRAFIALHGRYGEDGSVQGAL
ELMDLPYTGSGILASALAMDKWRTKMIWQAAGISTPDYVMLDADSNFQEVTDRLGLPLIIKPAREGSTIGLNKVDYAQDM
QSAYQTAAQHDSLVIAEQFIQGIELTAAILDDVPLPLVRIDVTEGLYDYQAKYFSESTRYTCPSGLSKILTTRIQEQALY
AHRILGCTGWSRVDLILDKNGQPFFLEANTSPGMTNHSLVPMAAQAAGISFDELVIQILELSCEYA

Sequences:

>Translated_306_residues
MNIRDLGKVAVLLGGRSAEREISLKSGHAVLAALQRSQVDAHAFDPVGQPLEDLLKQGFDRAFIALHGRYGEDGSVQGAL
ELMDLPYTGSGILASALAMDKWRTKMIWQAAGISTPDYVMLDADSNFQEVTDRLGLPLIIKPAREGSTIGLNKVDYAQDM
QSAYQTAAQHDSLVIAEQFIQGIELTAAILDDVPLPLVRIDVTEGLYDYQAKYFSESTRYTCPSGLSKILTTRIQEQALY
AHRILGCTGWSRVDLILDKNGQPFFLEANTSPGMTNHSLVPMAAQAAGISFDELVIQILELSCEYA
>Mature_306_residues
MNIRDLGKVAVLLGGRSAEREISLKSGHAVLAALQRSQVDAHAFDPVGQPLEDLLKQGFDRAFIALHGRYGEDGSVQGAL
ELMDLPYTGSGILASALAMDKWRTKMIWQAAGISTPDYVMLDADSNFQEVTDRLGLPLIIKPAREGSTIGLNKVDYAQDM
QSAYQTAAQHDSLVIAEQFIQGIELTAAILDDVPLPLVRIDVTEGLYDYQAKYFSESTRYTCPSGLSKILTTRIQEQALY
AHRILGCTGWSRVDLILDKNGQPFFLEANTSPGMTNHSLVPMAAQAAGISFDELVIQILELSCEYA

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=303, Percent_Identity=51.4851485148515, Blast_Score=311, Evalue=4e-86,
Organism=Escherichia coli, GI1786579, Length=353, Percent_Identity=32.0113314447592, Blast_Score=171, Evalue=4e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_NITEC (Q0AJE4)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_746492.1
- ProteinModelPortal:   Q0AJE4
- SMR:   Q0AJE4
- STRING:   Q0AJE4
- GeneID:   4272259
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_0243
- NMPDR:   fig|335283.3.peg.2116
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   RAFIILH
- PhylomeDB:   Q0AJE4
- ProtClustDB:   PRK01372
- BioCyc:   NEUT335283:NEUT_0243-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 33438; Mature: 33438

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRDLGKVAVLLGGRSAEREISLKSGHAVLAALQRSQVDAHAFDPVGQPLEDLLKQGFD
CCCHHHHHEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCHHCCCCCHHHHHHHHCCCC
RAFIALHGRYGEDGSVQGALELMDLPYTGSGILASALAMDKWRTKMIWQAAGISTPDYVM
EEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LDADSNFQEVTDRLGLPLIIKPAREGSTIGLNKVDYAQDMQSAYQTAAQHDSLVIAEQFI
EECCCCHHHHHHHCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QGIELTAAILDDVPLPLVRIDVTEGLYDYQAKYFSESTRYTCPSGLSKILTTRIQEQALY
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AHRILGCTGWSRVDLILDKNGQPFFLEANTSPGMTNHSLVPMAAQAAGISFDELVIQILE
HHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
LSCEYA
HHCCCC
>Mature Secondary Structure
MNIRDLGKVAVLLGGRSAEREISLKSGHAVLAALQRSQVDAHAFDPVGQPLEDLLKQGFD
CCCHHHHHEEEEECCCCCCCEEEECCCHHHHHHHHHHCCCCHHCCCCCHHHHHHHHCCCC
RAFIALHGRYGEDGSVQGALELMDLPYTGSGILASALAMDKWRTKMIWQAAGISTPDYVM
EEEEEEECCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEE
LDADSNFQEVTDRLGLPLIIKPAREGSTIGLNKVDYAQDMQSAYQTAAQHDSLVIAEQFI
EECCCCHHHHHHHCCCCEEEEECCCCCEECCCCHHHHHHHHHHHHHHHHCCCHHHHHHHH
QGIELTAAILDDVPLPLVRIDVTEGLYDYQAKYFSESTRYTCPSGLSKILTTRIQEQALY
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
AHRILGCTGWSRVDLILDKNGQPFFLEANTSPGMTNHSLVPMAAQAAGISFDELVIQILE
HHHHHCCCCCCEEEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCHHHHHHHHHH
LSCEYA
HHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA