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The map label for this gene is gmd [H]

Identifier: 114330187

GI number: 114330187

Start: 160144

End: 161175

Strand: Direct

Name: gmd [H]

Synonym: Neut_0156

Alternate gene names: 114330187

Gene position: 160144-161175 (Clockwise)

Preceding gene: 114330186

Following gene: 114330188

Centisome position: 6.02

GC content: 45.35

Gene sequence:

>1032_bases
ATGACCAAGAAAATACTGATTACCGGCATTACCGGGCAGGTTGGCTCGCAGCTTGCCGATTACATATTGGAGAACACACC
CTATGACGTAATTGGTCTGATGCGCTGGCAAGAGCCTATGGATAACCTTTACCATTTGACTGATCGGATCAACCGAAAAG
ACCGGATCAGCTTGTTTTATGCAGATCTCAATGATTATTCCGCGATTGCCAGGATGATCAGAGAAATCCAGCCTGAATAC
ATTTCACATCTGGCTGCGCAATCATACCCGAAGACTTCATTCAATATCCCTATTGAAACATTGCAGACAAACATTATTGG
TACAGCCAATCTGCTCGAGAATATTCGGCAACTGAAAGAAGTTGATGGTTATGATCCTGTGGTGCATATTTGTTCCTCAA
GTGAAGTGTATGGTCGGGCGAAGCCAGGAATTGCGTTATCGGAAGAGACTCAATTCCATGGTGCCAGTCCGTACAGTATC
AGTAAAATCGGTACAGATTATCTTGGGCAATTTTACGGAGAGGCTTATAGGATTCGTACGTTTGTTACTCGCATGGGTAC
GCATTCCGGCCCCAGGCGTAGCGATGTTTTTTTTGAGAGTACTGTTGCCAAGCAGATTGCTCTAATTGAAGCCGAGTACC
AGGAGCCGGTTATTCATGTTGGTAATTTATCGAGTACACGAACCTTTCAGGATGCGCGTGATGCGGTAAGAGCCTACTTT
CTGCTGCTGGAGGCAAGTGAGCAAGGCAAGATTCCTTGTGGGGAATATTTCAATATTGCTGGAGAGGAAGCTTTCAAACT
CCCGGAAGTGATTGAAATTTTGCTTGGGATGAGTACGCGGCGCGATATCCGGGTTATCACAGATCAGGAGCGGTTTCGTC
CCATTGATGCTGATTATCAGATGTTCGATAACACGAAAATTCGATCAACAATCAACTGGAAGCCGGAGATCCCGGCTCGT
CAGATGTTTGCAGATTTGCTGAATCACTGGCGGGAAGAAATTTCAAAAGGAAAAATTCCCTTGAATCGGTAA

Upstream 100 bases:

>100_bases
CCAATCTTTGCCAGACTACTCGGACGGCCGCCGCACAGCCTCTGTGAGGCAGCTCAATTAGAGTTTGCATTGCCATCAAA
GTAACTAACAAAAGAGTTTT

Downstream 100 bases:

>100_bases
GCACTTTAGTACGCTTTTTATTTTGCTCCTCACAGAATAATCCAGGTAATGACAATAATCAGAAGTCGGGCACCACTGCG
CCTCGGGCTGGCGGGTGGCG

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 343; Mature: 342

Protein sequence:

>343_residues
MTKKILITGITGQVGSQLADYILENTPYDVIGLMRWQEPMDNLYHLTDRINRKDRISLFYADLNDYSAIARMIREIQPEY
ISHLAAQSYPKTSFNIPIETLQTNIIGTANLLENIRQLKEVDGYDPVVHICSSSEVYGRAKPGIALSEETQFHGASPYSI
SKIGTDYLGQFYGEAYRIRTFVTRMGTHSGPRRSDVFFESTVAKQIALIEAEYQEPVIHVGNLSSTRTFQDARDAVRAYF
LLLEASEQGKIPCGEYFNIAGEEAFKLPEVIEILLGMSTRRDIRVITDQERFRPIDADYQMFDNTKIRSTINWKPEIPAR
QMFADLLNHWREEISKGKIPLNR

Sequences:

>Translated_343_residues
MTKKILITGITGQVGSQLADYILENTPYDVIGLMRWQEPMDNLYHLTDRINRKDRISLFYADLNDYSAIARMIREIQPEY
ISHLAAQSYPKTSFNIPIETLQTNIIGTANLLENIRQLKEVDGYDPVVHICSSSEVYGRAKPGIALSEETQFHGASPYSI
SKIGTDYLGQFYGEAYRIRTFVTRMGTHSGPRRSDVFFESTVAKQIALIEAEYQEPVIHVGNLSSTRTFQDARDAVRAYF
LLLEASEQGKIPCGEYFNIAGEEAFKLPEVIEILLGMSTRRDIRVITDQERFRPIDADYQMFDNTKIRSTINWKPEIPAR
QMFADLLNHWREEISKGKIPLNR
>Mature_342_residues
TKKILITGITGQVGSQLADYILENTPYDVIGLMRWQEPMDNLYHLTDRINRKDRISLFYADLNDYSAIARMIREIQPEYI
SHLAAQSYPKTSFNIPIETLQTNIIGTANLLENIRQLKEVDGYDPVVHICSSSEVYGRAKPGIALSEETQFHGASPYSIS
KIGTDYLGQFYGEAYRIRTFVTRMGTHSGPRRSDVFFESTVAKQIALIEAEYQEPVIHVGNLSSTRTFQDARDAVRAYFL
LLEASEQGKIPCGEYFNIAGEEAFKLPEVIEILLGMSTRRDIRVITDQERFRPIDADYQMFDNTKIRSTINWKPEIPARQ
MFADLLNHWREEISKGKIPLNR

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=345, Percent_Identity=27.8260869565217, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI7657641, Length=343, Percent_Identity=23.9067055393586, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI42516563, Length=338, Percent_Identity=23.3727810650888, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788366, Length=363, Percent_Identity=30.0275482093664, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI48994969, Length=332, Percent_Identity=29.5180722891566, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788353, Length=340, Percent_Identity=27.0588235294118, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=29.6187683284457, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=28.4457478005865, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=28.4457478005865, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=28.4457478005865, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=28.4457478005865, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=28.4457478005865, Blast_Score=133, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17539532, Length=344, Percent_Identity=23.8372093023256, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17568069, Length=340, Percent_Identity=24.7058823529412, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24158427, Length=349, Percent_Identity=29.7994269340974, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21356223, Length=340, Percent_Identity=24.4117647058824, Blast_Score=72, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 39290; Mature: 39159

Theoretical pI: Translated: 5.95; Mature: 5.95

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKILITGITGQVGSQLADYILENTPYDVIGLMRWQEPMDNLYHLTDRINRKDRISLFY
CCCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ADLNDYSAIARMIREIQPEYISHLAAQSYPKTSFNIPIETLQTNIIGTANLLENIRQLKE
ECCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
VDGYDPVVHICSSSEVYGRAKPGIALSEETQFHGASPYSISKIGTDYLGQFYGEAYRIRT
CCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHH
FVTRMGTHSGPRRSDVFFESTVAKQIALIEAEYQEPVIHVGNLSSTRTFQDARDAVRAYF
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
LLLEASEQGKIPCGEYFNIAGEEAFKLPEVIEILLGMSTRRDIRVITDQERFRPIDADYQ
HHEECCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHCCCCCCCEEEEECHHHCCCCCCCHH
MFDNTKIRSTINWKPEIPARQMFADLLNHWREEISKGKIPLNR
HHCCCEECEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TKKILITGITGQVGSQLADYILENTPYDVIGLMRWQEPMDNLYHLTDRINRKDRISLFY
CCEEEEEECCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
ADLNDYSAIARMIREIQPEYISHLAAQSYPKTSFNIPIETLQTNIIGTANLLENIRQLKE
ECCCHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCHHHHHHHHHHHHH
VDGYDPVVHICSSSEVYGRAKPGIALSEETQFHGASPYSISKIGTDYLGQFYGEAYRIRT
CCCCCCEEEEECCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHCHHHHHHHHHHHHHHHH
FVTRMGTHSGPRRSDVFFESTVAKQIALIEAEYQEPVIHVGNLSSTRTFQDARDAVRAYF
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHHHHHH
LLLEASEQGKIPCGEYFNIAGEEAFKLPEVIEILLGMSTRRDIRVITDQERFRPIDADYQ
HHEECCCCCCCCCCHHHCCCCHHHHCCHHHHHHHHCCCCCCCEEEEECHHHCCCCCCCHH
MFDNTKIRSTINWKPEIPARQMFADLLNHWREEISKGKIPLNR
HHCCCEECEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]