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The map label for this gene is ubiE [H]

Identifier: 114330096

GI number: 114330096

Start: 63958

End: 64845

Strand: Reverse

Name: ubiE [H]

Synonym: Neut_0065

Alternate gene names: 114330096

Gene position: 64845-63958 (Counterclockwise)

Preceding gene: 114330097

Following gene: 114330095

Centisome position: 2.44

GC content: 52.25

Gene sequence:

>888_bases
ATGGCAAGCCCGATTTGGCAAACTAAATTCGGGCTTTTTGCGAGATTGGAATGTGTGTGTGATCAGGAGAACTCAGTGAG
CCAACAATCAAACCTTAGTCTGGCAGAAGTATATGAACGCTACCTTAGCCGTGCTATCGCAGATCCTTGGACGCGCGTAC
TGCTTGAATTTGCATCACCCAAACCCGGTGAGCGAGTTCTGGATCTTGCCTGCGGTACGGGAAGTGTCGCACGGCAGGTC
GCTCCCATGGTTGGGGTAAGCGGTCAGGTCCTTGCTCTGGATATCAATTCAGAAATGCTGGATGTGGGTCGTGCACAACC
GCCGCCGGCTGGTGCTTCGATACTTTGGCAAGAGGGTGATGCAACTCGCCTCGAACTTCCCGATGACGCGTTTGATCTTG
TGCTGTGCCAACAAGGATTCCAGTTCTTTCCCGACCGAGTGGGTTCAGGTCGTGAAATGCGTCGCGTGCTACGCGATGGC
GGTAGCGCAGTTATCAGTGTCTGGCAGTCCCTGAACAGCCATCCTGTGTATGAGGCGTTGTTTACGGAAACCGCACGCCT
TCTTGATGTGCCCACCACTGACGTCGATGTTGCCTTTTCATTATCTAACTCCGAGGAGCTATACACATTGCTCAGCGAGG
CGGGCTTTCAATATATCGAAATTACTCCACGGGCGCTGTCTATTCGCATGTCTGAGCCAGAAAGGTTTGTTCAGTTTTCG
ATACTGGGCGCAGCCACGTCAGTGCCAGCGTTTGCATCTATGGATGCCGTAGAGCGCCTCGTTCTTGTGGAGGCAGTAAG
GGATGAGACGCACGCTATCGTGCAACACTTTCGGGATGGGGATTTTCTCGCGTTTTCTATGGAGACAAACATCGCGATTG
TAAAATAA

Upstream 100 bases:

>100_bases
GCTACTTATCCAGTTGGCCCCAATCCCAATGGGATCACATACCGCAGCATTCGGTGAGACCAGCACTTTCGATGTAGCCC
CGGTATGCTGGGGCTACTCA

Downstream 100 bases:

>100_bases
GCGACCCATGGTTTCTACTTTTCATACCCTCGTTTGTAAGACACGCTTTTCACTTGGTACAATCGTTCTTGCAATGAAGA
AATTTCTCCTTATTTTTATG

Product: methyltransferase type 11

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 294

Protein sequence:

>295_residues
MASPIWQTKFGLFARLECVCDQENSVSQQSNLSLAEVYERYLSRAIADPWTRVLLEFASPKPGERVLDLACGTGSVARQV
APMVGVSGQVLALDINSEMLDVGRAQPPPAGASILWQEGDATRLELPDDAFDLVLCQQGFQFFPDRVGSGREMRRVLRDG
GSAVISVWQSLNSHPVYEALFTETARLLDVPTTDVDVAFSLSNSEELYTLLSEAGFQYIEITPRALSIRMSEPERFVQFS
ILGAATSVPAFASMDAVERLVLVEAVRDETHAIVQHFRDGDFLAFSMETNIAIVK

Sequences:

>Translated_295_residues
MASPIWQTKFGLFARLECVCDQENSVSQQSNLSLAEVYERYLSRAIADPWTRVLLEFASPKPGERVLDLACGTGSVARQV
APMVGVSGQVLALDINSEMLDVGRAQPPPAGASILWQEGDATRLELPDDAFDLVLCQQGFQFFPDRVGSGREMRRVLRDG
GSAVISVWQSLNSHPVYEALFTETARLLDVPTTDVDVAFSLSNSEELYTLLSEAGFQYIEITPRALSIRMSEPERFVQFS
ILGAATSVPAFASMDAVERLVLVEAVRDETHAIVQHFRDGDFLAFSMETNIAIVK
>Mature_294_residues
ASPIWQTKFGLFARLECVCDQENSVSQQSNLSLAEVYERYLSRAIADPWTRVLLEFASPKPGERVLDLACGTGSVARQVA
PMVGVSGQVLALDINSEMLDVGRAQPPPAGASILWQEGDATRLELPDDAFDLVLCQQGFQFFPDRVGSGREMRRVLRDGG
SAVISVWQSLNSHPVYEALFTETARLLDVPTTDVDVAFSLSNSEELYTLLSEAGFQYIEITPRALSIRMSEPERFVQFSI
LGAATSVPAFASMDAVERLVLVEAVRDETHAIVQHFRDGDFLAFSMETNIAIVK

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG0500

COG function: function code QR; SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Escherichia coli, GI48994983, Length=209, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 32482; Mature: 32351

Theoretical pI: Translated: 4.29; Mature: 4.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASPIWQTKFGLFARLECVCDQENSVSQQSNLSLAEVYERYLSRAIADPWTRVLLEFASP
CCCCCCHHHHHHHHHHHEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
KPGERVLDLACGTGSVARQVAPMVGVSGQVLALDINSEMLDVGRAQPPPAGASILWQEGD
CCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCEEEEECCC
ATRLELPDDAFDLVLCQQGFQFFPDRVGSGREMRRVLRDGGSAVISVWQSLNSHPVYEAL
CEEEECCCCHHHHHHHHHHHHHCHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHH
FTETARLLDVPTTDVDVAFSLSNSEELYTLLSEAGFQYIEITPRALSIRMSEPERFVQFS
HHHHHHHEECCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCEEEEEECCCHHEEEEE
ILGAATSVPAFASMDAVERLVLVEAVRDETHAIVQHFRDGDFLAFSMETNIAIVK
EEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEC
>Mature Secondary Structure 
ASPIWQTKFGLFARLECVCDQENSVSQQSNLSLAEVYERYLSRAIADPWTRVLLEFASP
CCCCCHHHHHHHHHHHEEECCCCCCCHHHCCCHHHHHHHHHHHHHCCHHHHHHHHHCCC
KPGERVLDLACGTGSVARQVAPMVGVSGQVLALDINSEMLDVGRAQPPPAGASILWQEGD
CCCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEECCCHHHHCCCCCCCCCCCEEEEECCC
ATRLELPDDAFDLVLCQQGFQFFPDRVGSGREMRRVLRDGGSAVISVWQSLNSHPVYEAL
CEEEECCCCHHHHHHHHHHHHHCHHHCCCCHHHHHHHHCCCHHHHHHHHHCCCCCHHHHH
FTETARLLDVPTTDVDVAFSLSNSEELYTLLSEAGFQYIEITPRALSIRMSEPERFVQFS
HHHHHHHEECCCCCEEEEEEECCCHHHHHHHHHCCCEEEEECCCEEEEEECCCHHEEEEE
ILGAATSVPAFASMDAVERLVLVEAVRDETHAIVQHFRDGDFLAFSMETNIAIVK
EEHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA