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The map label for this gene is holB [H]

Identifier: 113970002

GI number: 113970002

Start: 1962170

End: 1963126

Strand: Direct

Name: holB [H]

Synonym: Shewmr4_1662

Alternate gene names: 113970002

Gene position: 1962170-1963126 (Clockwise)

Preceding gene: 113970001

Following gene: 113970003

Centisome position: 41.69

GC content: 47.34

Gene sequence:

>957_bases
ATGGTAACGCAAAGCGGATTTGATGTATTAACCCTGCCTTGGCTCGATGCGCCAAGACAGGCGTTTTTAACCCAGTTACA
AACTCAAAAAGTCCCCCACGCGCAACTCGTGGGTATCGATTCCGCCTATGGCGGTGAACTCTTGAGTGTGTTTATGGCGC
GCGCCGCCATGTGCTCACAACCCACTCCCACTGGCGGATGCGGTTTTTGTAAGTCCTGCCAGTTATTTGATGCGGGTAAT
CACCCTGATTTTTACCAAATTGAGGCCGATGGTCATCAAATTAAAGTCGACCAAATCCGTGAGCTGTGCACCCGCCTGAG
TGCAACCGCCCAGCAGAGTGGTCGGCGTGTCGCCATCATTCACCACAGCGAGCGCCTGAACTCAGCCTCCGCCAATGCGT
TACTCAAAACGCTCGAAGAGCCGGGCAAAGACACTTTACTGCTATTACATTCCGATACGCCCGCGCGTTTAATGGCAACG
ATCAGCAGTCGCTGCCAACGGCTTCCCTTTGTGGCGCCGTCTAAGATGTTGATCAAAAATTGGTTAATTCAGCACTGTCA
GATCCAAGAGGATGTGACTTGGTGTTTAAGTGTGGTTGGCGGCCCATTAAAGTTGGCCGAAAGTTTGCAAACAGATGGGA
CACAACCGAGTCGCTATCAAACTTTGTTAGGTTTTCGCAAAGATTGGGTGCAAAGTTTGTCGTCAGGCCATCTGTGTGCT
AGTTTGTTAACGATAAGTGAACAACAAATCATTGATGCTCTTAAGGTTTTATACTTACTCCTGAGACAAATTTTATTGAA
GTACAGCGACCAAGATGCGTTTGTGCAAGCGCAAATAGGGAACTTGGCGGCGAAAATCATGGGAATGTGTCATAGCTTAA
GTGCAATGCCGAGTGTCAATTATTTAGGCTTGTGTCAAAGTTATGTCTTAGAATATAGAGAAATAACAAATAAGTAA

Upstream 100 bases:

>100_bases
TCAAGCTTGCCAGCGAAGATGAGCAGATTGTCGTTATCGATGCCAGTCAAACCATGGCCGAAGTGCATAAAGATATTCTG
GCCGTGTTACAGGCGATGGC

Downstream 100 bases:

>100_bases
CATGCCATGGTCGATCTTGTCGTAAATTTTGATACCTTACATCAGCTTTACCGCGCCTATATGCCGTTTATCAAACCGGC
AGGGCTGTTTGTCGCCACTG

Product: DNA polymerase III subunit delta'

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MVTQSGFDVLTLPWLDAPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQPTPTGGCGFCKSCQLFDAGN
HPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMAT
ISSRCQRLPFVAPSKMLIKNWLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGTQPSRYQTLLGFRKDWVQSLSSGHLCA
SLLTISEQQIIDALKVLYLLLRQILLKYSDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVNYLGLCQSYVLEYREITNK

Sequences:

>Translated_318_residues
MVTQSGFDVLTLPWLDAPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQPTPTGGCGFCKSCQLFDAGN
HPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMAT
ISSRCQRLPFVAPSKMLIKNWLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGTQPSRYQTLLGFRKDWVQSLSSGHLCA
SLLTISEQQIIDALKVLYLLLRQILLKYSDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVNYLGLCQSYVLEYREITNK
>Mature_318_residues
MVTQSGFDVLTLPWLDAPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQPTPTGGCGFCKSCQLFDAGN
HPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAIIHHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMAT
ISSRCQRLPFVAPSKMLIKNWLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGTQPSRYQTLLGFRKDWVQSLSSGHLCA
SLLTISEQQIIDALKVLYLLLRQILLKYSDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVNYLGLCQSYVLEYREITNK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=181, Percent_Identity=33.7016574585635, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1786676, Length=136, Percent_Identity=30.1470588235294, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 35165; Mature: 35165

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTQSGFDVLTLPWLDAPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQ
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTPTGGCGFCKSCQLFDAGNHPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAII
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMATISSRCQRLPFVAPSKMLIKN
ECHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
WLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGTQPSRYQTLLGFRKDWVQSLSSGHLCA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
SLLTISEQQIIDALKVLYLLLRQILLKYSDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
YLGLCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVTQSGFDVLTLPWLDAPRQAFLTQLQTQKVPHAQLVGIDSAYGGELLSVFMARAAMCSQ
CCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCHHEEECCCCCCHHHHHHHHHHHHHHCC
PTPTGGCGFCKSCQLFDAGNHPDFYQIEADGHQIKVDQIRELCTRLSATAQQSGRRVAII
CCCCCCCCCCCCCCEEECCCCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHCCCEEEEE
HHSERLNSASANALLKTLEEPGKDTLLLLHSDTPARLMATISSRCQRLPFVAPSKMLIKN
ECHHHCCHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCHHHHHHH
WLIQHCQIQEDVTWCLSVVGGPLKLAESLQTDGTQPSRYQTLLGFRKDWVQSLSSGHLCA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHH
SLLTISEQQIIDALKVLYLLLRQILLKYSDQDAFVQAQIGNLAAKIMGMCHSLSAMPSVN
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCH
YLGLCQSYVLEYREITNK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]