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The map label for this gene is focA [H]

Identifier: 113969837

GI number: 113969837

Start: 1752609

End: 1753583

Strand: Direct

Name: focA [H]

Synonym: Shewmr4_1495

Alternate gene names: 113969837

Gene position: 1752609-1753583 (Clockwise)

Preceding gene: 113969834

Following gene: 113969838

Centisome position: 37.24

GC content: 48.62

Gene sequence:

>975_bases
ATGAAAGACTCACACGCTAACGCTCCACAAACATCATCGAATAGCCATGAGCCATTGCAGGTTTCTTCACACTTTAGCTG
TTATCACCAGGCCGAAATTTACGGCAAAAGCAAAGTGGTTAAAGCGCCTTGGCAATCCTTCGGTTTAGCGGTATTTGCTG
GCGCTTTTATTGCCTTAGCCTTTGTGTTTTATCTGACTGTCACTACGGGGGCGGGTGATAGCGCCTGGGGTTTAGTGCGC
CTGGTGGGTGGGATTGCCTTCAGCTTAGGCTTAATTTTAGTGGTGATTTGTGGTGGTGAGCTGTTTACCAGTTCAGTGCT
GAGCAGTGTTGCATGGGCCCAAAAACAGGTGACCACCGGTGAATTGCTCAAATGCTGGAGCCGCGTGTATATCGGCAACT
TCGTCGGCGCCATATTAATGGTTGGCTTGATCATGGCGGCGGGCTTATATGAGCTCGATGGCGGTAACTGGGGCTTAAAC
GCGCTAAAAGTGTCACAACATAAGCTGCATCATACTTGGGTGCAGGCATTCAGCTTAGGTATCTTATGTAACATGTTGGT
GTGTCTGGGCATTTGGATGACTTTCGCCAGCCGCGAATCCCTAACCAAAGCGATTCTACTGATCCTCCCAGTAGCCATGT
TTGTCAGCAGTGGCTTTGAGCACAGTATTGCAAACCTGTTTATGGTGCCGCTTGGGATCACGATTCAGTCGGTCGCCAGC
CCTGAGTTTTTTGCATCCCTAGGCGTGACCCAAGAGCAATTTGCCGATTTAACCGTGGCAAACTTTGTATTCAATAACTT
AATTCCCGTGACCCTAGGAAACATTGTCGGTGGCGGTGTGATCGTTGGCCTCGGTTATTGGTGGATTGAGCAAGGCCAAA
TTGCCTTACCCGATCCGCAAAAATCCCATCAGGCACACTTTTTTGCATCGCCTCTGCATACTGAAAAAGCGAAAAATAGC
CAAGAAAGTAAGTAA

Upstream 100 bases:

>100_bases
CTTAATGTAAGTTTTGATTCGTTTGATCTGCGCTTGGGCGCAGTAGGAGAGACCTAATGTTGCAAGTTCGCCTTTGTTTA
TCTGGTTTGGGAGTTCAGTT

Downstream 100 bases:

>100_bases
GAGAATTAGTCACTAAGCGAGTCAGTAACTAAGCGAGTCAGTCACATAAAAAATGCCCAGTGTTACTGGGCTTTTTTTGC
TGTATTCGTCGTAGCAGACA

Product: formate/nitrite transporter

Products: formate [Periplasm]; formate [Cytoplasm] [C]

Alternate protein names: Formate channel 1 [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKDSHANAPQTSSNSHEPLQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDSAWGLVR
LVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTGELLKCWSRVYIGNFVGAILMVGLIMAAGLYELDGGNWGLN
ALKVSQHKLHHTWVQAFSLGILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQKSHQAHFFASPLHTEKAKNS
QESK

Sequences:

>Translated_324_residues
MKDSHANAPQTSSNSHEPLQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDSAWGLVR
LVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTGELLKCWSRVYIGNFVGAILMVGLIMAAGLYELDGGNWGLN
ALKVSQHKLHHTWVQAFSLGILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQKSHQAHFFASPLHTEKAKNS
QESK
>Mature_324_residues
MKDSHANAPQTSSNSHEPLQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALAFVFYLTVTTGAGDSAWGLVR
LVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTGELLKCWSRVYIGNFVGAILMVGLIMAAGLYELDGGNWGLN
ALKVSQHKLHHTWVQAFSLGILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQKSHQAHFFASPLHTEKAKNS
QESK

Specific function: Involved in the bidirectional transport of formate [H]

COG id: COG2116

COG function: function code P; Formate/nitrite family of transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FNT transporter (TC 2.A.44) family [H]

Homologues:

Organism=Escherichia coli, GI1787132, Length=259, Percent_Identity=55.2123552123552, Blast_Score=294, Evalue=6e-81,
Organism=Escherichia coli, GI1788837, Length=263, Percent_Identity=39.9239543726236, Blast_Score=194, Evalue=6e-51,
Organism=Escherichia coli, GI87082249, Length=246, Percent_Identity=31.3008130081301, Blast_Score=79, Evalue=4e-16,
Organism=Saccharomyces cerevisiae, GI6321779, Length=206, Percent_Identity=30.5825242718447, Blast_Score=95, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000292 [H]

Pfam domain/function: PF01226 Form_Nir_trans [H]

EC number: NA

Molecular weight: Translated: 34882; Mature: 34882

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: PS01005 FORMATE_NITRITE_TP_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDSHANAPQTSSNSHEPLQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALA
CCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
FVFYLTVTTGAGDSAWGLVRLVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHH
ELLKCWSRVYIGNFVGAILMVGLIMAAGLYELDGGNWGLNALKVSQHKLHHTWVQAFSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCC
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQ
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHEECHHHEEECCCEECCCCC
KSHQAHFFASPLHTEKAKNSQESK
CCCCCCEECCCHHHHHHCCCCCCC
>Mature Secondary Structure
MKDSHANAPQTSSNSHEPLQVSSHFSCYHQAEIYGKSKVVKAPWQSFGLAVFAGAFIALA
CCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHHH
FVFYLTVTTGAGDSAWGLVRLVGGIAFSLGLILVVICGGELFTSSVLSSVAWAQKQVTTG
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCHH
ELLKCWSRVYIGNFVGAILMVGLIMAAGLYELDGGNWGLNALKVSQHKLHHTWVQAFSLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
ILCNMLVCLGIWMTFASRESLTKAILLILPVAMFVSSGFEHSIANLFMVPLGITIQSVAS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHCC
PEFFASLGVTQEQFADLTVANFVFNNLIPVTLGNIVGGGVIVGLGYWWIEQGQIALPDPQ
HHHHHHHCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHEECHHHEEECCCEECCCCC
KSHQAHFFASPLHTEKAKNSQESK
CCCCCCEECCCHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate [Cytoplasm]; formate [Periplasm] [C]

Specific reaction: formate [Cytoplasm] = formate [Periplasm] formate [Periplasm] = formate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]