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The map label for this gene is rlmI [H]

Identifier: 113969828

GI number: 113969828

Start: 1741724

End: 1742635

Strand: Direct

Name: rlmI [H]

Synonym: Shewmr4_1486

Alternate gene names: 113969828

Gene position: 1741724-1742635 (Clockwise)

Preceding gene: 113969827

Following gene: 113969830

Centisome position: 37.01

GC content: 50.44

Gene sequence:

>912_bases
ATGCAACGTTTTATAGATGCACTGGCGACGATAGAAGTCGGTGAAGATGCCACGCGATTATTCCATGGCCGTGGCGGTTA
TTACCCTGGCTGCGAACATCTTTGTTTAGATTGGTTCGCGCCCGTGTTGTTATTGACCAGTTTTATTCCCCTCGAAGCGG
CCGAGTTGGCCCTGTGCCAACAGGCGATTACGGCGCGTTGGCAAGCGATACAGCCCAATCGGCCGTTAAACTTGGTGTAT
CAATATCGTAGTGGTGGCGAAACCTTTAGCCAAGTACTGGCGGGTGAGGTGCCCGATCCCCATGTGGTCACTGAAAATGG
TGCCCAGTTTCAGGTACATTTATTGCGGGGACAAAACCACGGTTTGTTCCTCGATATGGCCAATGGCCGTGAATGGGTAA
GAGCCCATGCTCGCCATAAGAAAGTGCTGAATTTGTTTGCTTATACCTGTGGTTTCTCGGTGGCAGCACTGCAGGGCGGT
GCCGATGAAGTGGTGAACATGGACATGAGTAAAGGCGCGTTAGCCATAGGCAAGCAGAATCATCTTCTCAATGGCTTTAG
CGCGGGCGCACGCTTCTTAGGGCACGATATTTTTAAGTCATGGGGTAAGTTACGCAAGTTAGGCCCCTATGACATTGTGG
TTGCCGATCCGCCGAGTAATCAAAAGGGCAGTTTCGTTGCGACTAAGGATTATGTGCGTTTGATCAGGCATTTACCCGAG
TTGTTAGCCGAGAATGCCGAAGTGCTACTCTGCTTAAATGCACCGGAACTCGATACCCAGTTTTTACGTCAGCAAGTCGA
AGACGCCGCGCTTGCACTCGAATACCTTGAGCGCTTACCCAATCCCGCTGCCTTTGTTGATGTCTCTGAGGAGAAATCAT
TAAAGGTGCTACGTTATCGCTTAAAGGCGTAA

Upstream 100 bases:

>100_bases
ATCCGACGATGTGGATGATAAACTAGTAAGCCAATCGACAAGCACCGCTCCGATTTCTGAACCTGTCGATACCCATTCAA
CAGATAATAAGTAGTAGATA

Downstream 100 bases:

>100_bases
GCCCTCGGGTCTCATTAAACGCCGTCATGAAATGCCATCATAAGACCTTAAGAGGCTTTATGATGGCGTCGAATGATGTC
GCTTCGCGCCTTATCCTATC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m5C1962 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase RlmI [H]

Number of amino acids: Translated: 303; Mature: 303

Protein sequence:

>303_residues
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLEAAELALCQQAITARWQAIQPNRPLNLVY
QYRSGGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNHGLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
LLAENAEVLLCLNAPELDTQFLRQQVEDAALALEYLERLPNPAAFVDVSEEKSLKVLRYRLKA

Sequences:

>Translated_303_residues
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLEAAELALCQQAITARWQAIQPNRPLNLVY
QYRSGGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNHGLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
LLAENAEVLLCLNAPELDTQFLRQQVEDAALALEYLERLPNPAAFVDVSEEKSLKVLRYRLKA
>Mature_303_residues
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLEAAELALCQQAITARWQAIQPNRPLNLVY
QYRSGGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNHGLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGG
ADEVVNMDMSKGALAIGKQNHLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
LLAENAEVLLCLNAPELDTQFLRQQVEDAALALEYLERLPNPAAFVDVSEEKSLKVLRYRLKA

Specific function: Specifically methylates the cytosine at position 1962 (m5C1962) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Escherichia coli, GI145693111, Length=281, Percent_Identity=30.6049822064057, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1787181, Length=233, Percent_Identity=29.1845493562232, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002478
- InterPro:   IPR015947
- InterPro:   IPR019614 [H]

Pfam domain/function: PF10672 Methyltrans_SAM [H]

EC number: =2.1.1.191 [H]

Molecular weight: Translated: 33707; Mature: 33707

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLEAAELALCQ
CHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
QAITARWQAIQPNRPLNLVYQYRSGGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNH
HHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEECCCCEEEEEEEECCCC
GLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALAIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHCCCCCCCEEEECCCC
HLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLAENAEVLLCLNAPELDTQFLRQQVEDAALALEYLERLPNPAAFVDVSEEKSLKVLRYR
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
LKA
HCC
>Mature Secondary Structure
MQRFIDALATIEVGEDATRLFHGRGGYYPGCEHLCLDWFAPVLLLTSFIPLEAAELALCQ
CHHHHHHHHHHCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
QAITARWQAIQPNRPLNLVYQYRSGGETFSQVLAGEVPDPHVVTENGAQFQVHLLRGQNH
HHHHHHHHCCCCCCCEEEEEEECCCCHHHHHHHHCCCCCCCEEECCCCEEEEEEEECCCC
GLFLDMANGREWVRAHARHKKVLNLFAYTCGFSVAALQGGADEVVNMDMSKGALAIGKQN
CEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCHHEECCCCHHHHHCCCCCCCEEEECCCC
HLLNGFSAGARFLGHDIFKSWGKLRKLGPYDIVVADPPSNQKGSFVATKDYVRLIRHLPE
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEHHHHHHHHHHHHH
LLAENAEVLLCLNAPELDTQFLRQQVEDAALALEYLERLPNPAAFVDVSEEKSLKVLRYR
HHHCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHH
LKA
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA