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The map label for this gene is glpE [C]

Identifier: 113969729

GI number: 113969729

Start: 1614698

End: 1615057

Strand: Direct

Name: glpE [C]

Synonym: Shewmr4_1387

Alternate gene names: 113969729

Gene position: 1614698-1615057 (Clockwise)

Preceding gene: 113969728

Following gene: 113969730

Centisome position: 34.31

GC content: 52.78

Gene sequence:

>360_bases
ATGAATCCATCCAGTGCTTTTTCAGCCCTTGTCGAATCGATTCGACCCCATGTCGTTGAAGTGAGTATTGAGGCCTACCA
AGCCGATGACACTTGGGTATTGATTGATGTCCGTGAAGATAACGAGTGGCTACAGGATCATCTGCCGCAGGCGAAACATA
TGAGCCGCGGCATTATTGAGCGCGATATTGAACAGCGCTTCCCCGATAAACATACGCCTCTGCTGCTCTATTGCGCGGGC
GGCGCACGCTCTGTACTGGCGGCCAGCAGTTTGCAACTGATGGGTTACCAAAGGGTGGCCTCCTTGATTGGGGGTTATAA
AGGCTGGATCCAACGCCAGCTGCCCGTGGTGCAAGATTAA

Upstream 100 bases:

>100_bases
TACACGCCGTTAATGGCAATGATTGCGTCTCCCCCAGTAGCGAATTACACTTCAACTTATATTCGTTAAGTCATTCAATC
CCTTATCTAAGGAGTCTCTG

Downstream 100 bases:

>100_bases
TTTAGGCTGGGTTTAACTTTTTATAGGCGAGTCACATGCAATACTTCCCGCTGTTTATTGATACTGTTAATTTAAATGTC
CTGCTGGTGGGAGCGGGTGA

Product: rhodanese domain-containing protein

Products: H+; Sulfite; Thiocyanate [C]

Alternate protein names: Rhodanese Domain Protein; Rhodanese-Like Protein; Molybdopterin Biosynthesis Protein; Molybdopterin Biosynthesis Protein MoeB; Rhodanese-Like Domain-Containing Protein; Rhodanese-Related Sulfurtransferase; Thiosulfate Sulfurtransferase; Molybdopterin Biosynthesis MoeB Protein; Rhodanese-Like Family Protein; Thiosulfate Sulfurtransferase GlpE; Rhodanese-Like Domain Protein; Metallo-Beta-Lactamase Superfamily Protein; Rhodanese/Sulfurtransferase-Like Protein; Rhodanese/Moeb/Thif Domain Protein

Number of amino acids: Translated: 119; Mature: 119

Protein sequence:

>119_residues
MNPSSAFSALVESIRPHVVEVSIEAYQADDTWVLIDVREDNEWLQDHLPQAKHMSRGIIERDIEQRFPDKHTPLLLYCAG
GARSVLAASSLQLMGYQRVASLIGGYKGWIQRQLPVVQD

Sequences:

>Translated_119_residues
MNPSSAFSALVESIRPHVVEVSIEAYQADDTWVLIDVREDNEWLQDHLPQAKHMSRGIIERDIEQRFPDKHTPLLLYCAG
GARSVLAASSLQLMGYQRVASLIGGYKGWIQRQLPVVQD
>Mature_119_residues
MNPSSAFSALVESIRPHVVEVSIEAYQADDTWVLIDVREDNEWLQDHLPQAKHMSRGIIERDIEQRFPDKHTPLLLYCAG
GARSVLAASSLQLMGYQRVASLIGGYKGWIQRQLPVVQD

Specific function: Catalyzes, Although With Low Efficiency, The Sulfur Transfer Reaction From Thiosulfate To Cyanide. The Relatively Low Affinity Of Glpe For Both Thiosulfate And Cyanide Suggests That These Compounds Are Not The Physiological Substrates. Thioredoxin 1 Or Re

COG id: COG0607

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.8.1.1 [C]

Molecular weight: Translated: 13429; Mature: 13429

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSSAFSALVESIRPHVVEVSIEAYQADDTWVLIDVREDNEWLQDHLPQAKHMSRGIIE
CCCHHHHHHHHHHHCCEEEEEEEEEEECCCCEEEEEEECCCHHHHHCCCHHHHHHHHHHH
RDIEQRFPDKHTPLLLYCAGGARSVLAASSLQLMGYQRVASLIGGYKGWIQRQLPVVQD
HHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNPSSAFSALVESIRPHVVEVSIEAYQADDTWVLIDVREDNEWLQDHLPQAKHMSRGIIE
CCCHHHHHHHHHHHCCEEEEEEEEEEECCCCEEEEEEECCCHHHHHCCCHHHHHHHHHHH
RDIEQRFPDKHTPLLLYCAGGARSVLAASSLQLMGYQRVASLIGGYKGWIQRQLPVVQD
HHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Cyanide; Thiosulfate [C]

Specific reaction: Cyanide + Thiosulfate --> H+ + Sulfite + Thiocyanate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA