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The map label for this gene is ada [H]

Identifier: 113969709

GI number: 113969709

Start: 1594653

End: 1596281

Strand: Direct

Name: ada [H]

Synonym: Shewmr4_1367

Alternate gene names: 113969709

Gene position: 1594653-1596281 (Clockwise)

Preceding gene: 113969707

Following gene: 113969710

Centisome position: 33.88

GC content: 50.95

Gene sequence:

>1629_bases
ATGAAGCAGATAACCGTGAGCCTAAAGCCAAGTTTCAAACCGCCATTAGTGCAAACATCAAGTGACTTGCAGGATTGCCA
AATATCCGCCGGGATTTGCCATGAAGCGCGCATGAGTCGCGATCCGCGTTTTGATGGTAAGTTCTTTGTGGGCGTGTTAA
GTACGGGGATTTATTGCCGAACAGTATGCCCAGCAGTGGCGCCGAAGGAAGAAAATGTTCGTTATTTTGACTCGGCCATT
AAAGCTGCGCAGGCGGGACTTAGACCTTGCCTGCGTTGCCGCCCCGATAGCGCCCCTGGTTCTAATCCTTGGAAAGGTAC
TAACACTACCTTAGACAGAGCGATAGGCTTGATTGAAGCTGGCACCTTATCTGGCGAACAAGCATTAACGGTTGAGGCCT
TGGCAGATAAACTGGGGATCAGCAGCCGTTATTTAAATAAATTATTTACCGCAGGATTTGGCACATCACCAAAGCAATTT
GCCCTCTATCGTCAGTTATTGTTCGCAAAACAGTTATTGCATCAAACGCAGTTGCCCATCACTCAGGTCGCCCTTGCCGC
AGGTTTTAACAGTATTCGTCGTTTCAACGAGGCGTTTCAGCAGGCATTGCAATTAACGCCTACGCAGTTACGAAAATCGA
GCCAGAAGTCGACGACGAAAATAGACGACGGGGAGAGTGTCGAACTCGTTTGTTCGCAGGAGATGCCAGCGCATAGCTTG
AGTCTGTATCAGTACTACCGGCCGCCACTCGATTGGTCGGCGCAATTAGCCTTTTATCGCCTGCGCGCCGTCGCTGGCAT
GGAATGGTTTAGTGAAACAGAAGACTCTCACGCCCATGGGGCGATTGACCAAGATACGCCACTTGAGTATGGCCGCACTC
TGCAACTTGAGGATATCCGCGCTGTGGTGCATATCGTCCATGAGGCGTCTTTGCATCGCTTTAAAATCACGCTGACGTTA
ACGCCCGACTCGCCCTTATCGGGTCTACAAAAGCTCATCACCCAAGTGCGGCGCATTTTAGATCTCGATGCCGACATGCA
GCAGATTGAACACAATTTAGAGCACTTGACCGATCTTAAACTCAGTAGCAAATCCGGGCTTAGGATCCCGGGCGCTGGCA
CTGTGTTTGAGGCGGGTTGCCGAGCGGTCTTAGGCCAGCAGGTGAGCGTAGTGCAGGCCACTAAATTACTGAATATATTG
GTCGAAGCCTATGGCGAGCGCTTTAGTTTAAACGGGCGGGAATATCGACTATTCCCCACGCCGCAGGCGATTCGTGAGGC
GAGTCTCGATGAGCTTAAAATGCCCGGTGCGCGCAAATTGGCGCTCAATGCGCTTGCGGCGTTTATCTGTGAGCAGCCCG
AAGCCTCAGTCGATGAGTGGATTGGGGTAAAAGGCATCGGCCCTTGGACCATTGCCTATGCCAAACTGCGAGGACTTGGC
GATCCGAATATCTTCTTGCACACGGATTTAATTGTTAAAAAACAATTGTTAGCCAGTGTTGCCGCGCAAAAAGGCGTGGA
TATTGAAGCGGTAAAGCAGCTGGATTATACAACGCTTGCACAGCGGGTGAGCGCGGATATCGCCCCTTGGGGGAGTTATT
TAACTTTCCAGCTGTGGTCCAATGCATAA

Upstream 100 bases:

>100_bases
ACGTGATGATCCAGTTCCGTTTTCCGCGAGTGTGCTAGCATAGGGTGACGTACAGTAACCGACAAGTACAAAATTGTGAA
TAAGATTGTCGGATAACTCA

Downstream 100 bases:

>100_bases
GACATTTCAGGCCACTCGTTATGGATCCAGTACCTAGGCTCCATATGAGAGCCACTAATAGAAACATTATTTGAAACACA
GTACGAAAACATAGTACGAA

Product: transcriptional regulator Ada / DNA-3-methyladenine glycosylase II / DNA-O6-methylguanine--protein-cysteine S-methyltransferase

Products: NA

Alternate protein names: Regulatory protein of adaptative response; Methylated-DNA--protein-cysteine methyltransferase; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 542; Mature: 542

Protein sequence:

>542_residues
MKQITVSLKPSFKPPLVQTSSDLQDCQISAGICHEARMSRDPRFDGKFFVGVLSTGIYCRTVCPAVAPKEENVRYFDSAI
KAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEAGTLSGEQALTVEALADKLGISSRYLNKLFTAGFGTSPKQF
ALYRQLLFAKQLLHQTQLPITQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTTKIDDGESVELVCSQEMPAHSL
SLYQYYRPPLDWSAQLAFYRLRAVAGMEWFSETEDSHAHGAIDQDTPLEYGRTLQLEDIRAVVHIVHEASLHRFKITLTL
TPDSPLSGLQKLITQVRRILDLDADMQQIEHNLEHLTDLKLSSKSGLRIPGAGTVFEAGCRAVLGQQVSVVQATKLLNIL
VEAYGERFSLNGREYRLFPTPQAIREASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
DPNIFLHTDLIVKKQLLASVAAQKGVDIEAVKQLDYTTLAQRVSADIAPWGSYLTFQLWSNA

Sequences:

>Translated_542_residues
MKQITVSLKPSFKPPLVQTSSDLQDCQISAGICHEARMSRDPRFDGKFFVGVLSTGIYCRTVCPAVAPKEENVRYFDSAI
KAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEAGTLSGEQALTVEALADKLGISSRYLNKLFTAGFGTSPKQF
ALYRQLLFAKQLLHQTQLPITQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTTKIDDGESVELVCSQEMPAHSL
SLYQYYRPPLDWSAQLAFYRLRAVAGMEWFSETEDSHAHGAIDQDTPLEYGRTLQLEDIRAVVHIVHEASLHRFKITLTL
TPDSPLSGLQKLITQVRRILDLDADMQQIEHNLEHLTDLKLSSKSGLRIPGAGTVFEAGCRAVLGQQVSVVQATKLLNIL
VEAYGERFSLNGREYRLFPTPQAIREASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
DPNIFLHTDLIVKKQLLASVAAQKGVDIEAVKQLDYTTLAQRVSADIAPWGSYLTFQLWSNA
>Mature_542_residues
MKQITVSLKPSFKPPLVQTSSDLQDCQISAGICHEARMSRDPRFDGKFFVGVLSTGIYCRTVCPAVAPKEENVRYFDSAI
KAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEAGTLSGEQALTVEALADKLGISSRYLNKLFTAGFGTSPKQF
ALYRQLLFAKQLLHQTQLPITQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTTKIDDGESVELVCSQEMPAHSL
SLYQYYRPPLDWSAQLAFYRLRAVAGMEWFSETEDSHAHGAIDQDTPLEYGRTLQLEDIRAVVHIVHEASLHRFKITLTL
TPDSPLSGLQKLITQVRRILDLDADMQQIEHNLEHLTDLKLSSKSGLRIPGAGTVFEAGCRAVLGQQVSVVQATKLLNIL
VEAYGERFSLNGREYRLFPTPQAIREASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
DPNIFLHTDLIVKKQLLASVAAQKGVDIEAVKQLDYTTLAQRVSADIAPWGSYLTFQLWSNA

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus [H]

COG id: COG2169

COG function: function code F; Adenosine deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788383, Length=310, Percent_Identity=30, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1788542, Length=181, Percent_Identity=32.5966850828729, Blast_Score=89, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR010316
- InterPro:   IPR011257
- InterPro:   IPR003265
- InterPro:   IPR003583
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR023170
- InterPro:   IPR012294 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF06029 AlkA_N; PF00730 HhH-GPD [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 59870; Mature: 59870

Theoretical pI: Translated: 8.15; Mature: 8.15

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQITVSLKPSFKPPLVQTSSDLQDCQISAGICHEARMSRDPRFDGKFFVGVLSTGIYCR
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCEEHHHHHCCHHHH
TVCPAVAPKEENVRYFDSAIKAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
GTLSGEQALTVEALADKLGISSRYLNKLFTAGFGTSPKQFALYRQLLFAKQLLHQTQLPI
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
TQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTTKIDDGESVELVCSQEMPAHSL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHH
SLYQYYRPPLDWSAQLAFYRLRAVAGMEWFSETEDSHAHGAIDQDTPLEYGRTLQLEDIR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHH
AVVHIVHEASLHRFKITLTLTPDSPLSGLQKLITQVRRILDLDADMQQIEHNLEHLTDLK
HHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
LSSKSGLRIPGAGTVFEAGCRAVLGQQVSVVQATKLLNILVEAYGERFSLNGREYRLFPT
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCC
PQAIREASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
HHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DPNIFLHTDLIVKKQLLASVAAQKGVDIEAVKQLDYTTLAQRVSADIAPWGSYLTFQLWS
CCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
NA
CC
>Mature Secondary Structure
MKQITVSLKPSFKPPLVQTSSDLQDCQISAGICHEARMSRDPRFDGKFFVGVLSTGIYCR
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHCCHHHHHCCCCCCCCCCEEHHHHHCCHHHH
TVCPAVAPKEENVRYFDSAIKAAQAGLRPCLRCRPDSAPGSNPWKGTNTTLDRAIGLIEA
HHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCC
GTLSGEQALTVEALADKLGISSRYLNKLFTAGFGTSPKQFALYRQLLFAKQLLHQTQLPI
CCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCH
TQVALAAGFNSIRRFNEAFQQALQLTPTQLRKSSQKSTTKIDDGESVELVCSQEMPAHSL
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHH
SLYQYYRPPLDWSAQLAFYRLRAVAGMEWFSETEDSHAHGAIDQDTPLEYGRTLQLEDIR
HHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEEHHHHH
AVVHIVHEASLHRFKITLTLTPDSPLSGLQKLITQVRRILDLDADMQQIEHNLEHLTDLK
HHHHHHHHHCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
LSSKSGLRIPGAGTVFEAGCRAVLGQQVSVVQATKLLNILVEAYGERFSLNGREYRLFPT
CCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEEECCCCEEEECCC
PQAIREASLDELKMPGARKLALNALAAFICEQPEASVDEWIGVKGIGPWTIAYAKLRGLG
HHHHHHCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHHHHHHHHCCC
DPNIFLHTDLIVKKQLLASVAAQKGVDIEAVKQLDYTTLAQRVSADIAPWGSYLTFQLWS
CCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEC
NA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]