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The map label for this gene is flgJ [H]

Identifier: 113969611

GI number: 113969611

Start: 1473082

End: 1474236

Strand: Direct

Name: flgJ [H]

Synonym: Shewmr4_1267

Alternate gene names: 113969611

Gene position: 1473082-1474236 (Clockwise)

Preceding gene: 113969610

Following gene: 113969612

Centisome position: 31.3

GC content: 50.04

Gene sequence:

>1155_bases
ATGGAAAAGCTTTCAAGTTCATCACATTTTCTTGATCTGGGCGGGCTCGACTCCCTGCGCGCCCAGGCTCAGAAAGATGA
GAAGGGCGCCTTGAAAAAAGTCGCCCAACAATTTGAAGGCGTTTTCGTGCAAATGCTGATGAAGAGCATGCGTGACGCCA
ACGCAGTGTTTGAATCCGACAGCCCACTCAACAGCCAATACACTAAATTTTATGAGCAAATGCGGGACCAGCAGTTATCC
GTCGATTTATCGGATAAAGGTGTGCTGGGCCTTGCAGACATGATGGTGCAGCAATTATCCCCCGAGTCGAGTCAGTTTAC
GCCCGCATCCGTGCTGCGTAATGACGGTGGTATGAAGCTGCAACATGATGCGAAAGCCTTCAATATTCCAGCTCAAGCAA
CCTCGGCAGCTGAAACTCAAACTGCGGCTGCGCCAGTCGTTGCCGCGCAAGGTGTTCCGGCATCGATTGCACGCCCAAGT
GCTAACGTGGATAACGGCGATGGCGTGACGTCCTCCTTAGATATTGATAGACCTGAGCGTTTGCTGGCCATAGATACGCC
AAAGCCCGCTTGGTCAGAGCAACCCTTATCGCCAATCGAACCTGTGATCAGTGGCCAAATCTTGCCGACGGCGGCATTTA
GGGAAACACAAAAGACCCTCAAATTTGGTAGTCGGGAAGAGTTTTTGGCGACCCTCTATCCTCATGCAGAAAAAGCCGCC
AAAGCCCTTGGCACTCAACCCGAAGTGCTCTTGGCACAGTCAGCCCTTGAGACGGGCTGGGGTCAAAAAATTGTCCGTGG
CAATAATGGCGCGCCAAGCCATAACCTGTTCAATATTAAAGCCGATCGTCGCTGGCAAGGTGATAAGGCTAACGTGAGTA
CCTTGGAATTTGAGCAGGGTATTGCCGTTAGACAAAAAGCCGATTTTAGGGTGTATGCCGATTTCGAGCACAGTTTTAAC
GATTTTGTTTCCTTTATTGCTGAGGGTGAGCGTTATCAGGCGGCGAAGAAAGTCGCGGCGTCACCAACTCAGTTTATTCG
AGCATTACAGGATGCTGGTTATGCCACCGATCCTAAATATGCTGAAAAAGTCATTAAAGTTATGCAGTCCATCAGCGAAG
AGCTGAAATCGATACTGCCAGGAGAAGACAAATGA

Upstream 100 bases:

>100_bases
GCGCGGCCCCTTCAGATGTACTTGCTATCCTCGAAGCGTTAAAAGTGGCAGGTGCTTTGCATGGTGAACTTATCATCATC
TAATTTCTGTCGGGAAAATC

Downstream 100 bases:

>100_bases
TCATTCTTCATAGGATGTTAGTGTTGGGGAGTATCGGCTAATGGCTATCGATCTATTAAATATTGCCCGTACAGGCGTAC
TCGCATCCCAGTCACAACTG

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGVFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQFTPASVLRNDGGMKLQHDAKAFNIPAQATSAAETQTAAAPVVAAQGVPASIARPS
ANVDNGDGVTSSLDIDRPERLLAIDTPKPAWSEQPLSPIEPVISGQILPTAAFRETQKTLKFGSREEFLATLYPHAEKAA
KALGTQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEQGIAVRQKADFRVYADFEHSFN
DFVSFIAEGERYQAAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQSISEELKSILPGEDK

Sequences:

>Translated_384_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGVFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQFTPASVLRNDGGMKLQHDAKAFNIPAQATSAAETQTAAAPVVAAQGVPASIARPS
ANVDNGDGVTSSLDIDRPERLLAIDTPKPAWSEQPLSPIEPVISGQILPTAAFRETQKTLKFGSREEFLATLYPHAEKAA
KALGTQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEQGIAVRQKADFRVYADFEHSFN
DFVSFIAEGERYQAAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQSISEELKSILPGEDK
>Mature_384_residues
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGVFVQMLMKSMRDANAVFESDSPLNSQYTKFYEQMRDQQLS
VDLSDKGVLGLADMMVQQLSPESSQFTPASVLRNDGGMKLQHDAKAFNIPAQATSAAETQTAAAPVVAAQGVPASIARPS
ANVDNGDGVTSSLDIDRPERLLAIDTPKPAWSEQPLSPIEPVISGQILPTAAFRETQKTLKFGSREEFLATLYPHAEKAA
KALGTQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEQGIAVRQKADFRVYADFEHSFN
DFVSFIAEGERYQAAKKVAASPTQFIRALQDAGYATDPKYAEKVIKVMQSISEELKSILPGEDK

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=187, Percent_Identity=41.1764705882353, Blast_Score=133, Evalue=2e-32,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR019301
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.-

Molecular weight: Translated: 41898; Mature: 41898

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGVFVQMLMKSMRDANAVFESD
CCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPESSQFTPASVLRNDGGMKL
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEE
QHDAKAFNIPAQATSAAETQTAAAPVVAAQGVPASIARPSANVDNGDGVTSSLDIDRPER
ECCCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
LLAIDTPKPAWSEQPLSPIEPVISGQILPTAAFRETQKTLKFGSREEFLATLYPHAEKAA
EEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
KALGTQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEQG
HHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEHHCC
IAVRQKADFRVYADFEHSFNDFVSFIAEGERYQAAKKVAASPTQFIRALQDAGYATDPKY
CCEEECCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHH
AEKVIKVMQSISEELKSILPGEDK
HHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MEKLSSSSHFLDLGGLDSLRAQAQKDEKGALKKVAQQFEGVFVQMLMKSMRDANAVFESD
CCCCCCCCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SPLNSQYTKFYEQMRDQQLSVDLSDKGVLGLADMMVQQLSPESSQFTPASVLRNDGGMKL
CCCCHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHCCCCCEE
QHDAKAFNIPAQATSAAETQTAAAPVVAAQGVPASIARPSANVDNGDGVTSSLDIDRPER
ECCCCCCCCCHHHHHHHHHHHHHCCHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCC
LLAIDTPKPAWSEQPLSPIEPVISGQILPTAAFRETQKTLKFGSREEFLATLYPHAEKAA
EEEECCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHH
KALGTQPEVLLAQSALETGWGQKIVRGNNGAPSHNLFNIKADRRWQGDKANVSTLEFEQG
HHHCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCEEEEHHCC
IAVRQKADFRVYADFEHSFNDFVSFIAEGERYQAAKKVAASPTQFIRALQDAGYATDPKY
CCEEECCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCHHH
AEKVIKVMQSISEELKSILPGEDK
HHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]