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The map label for this gene is phrA [H]

Identifier: 113969509

GI number: 113969509

Start: 1371647

End: 1373128

Strand: Direct

Name: phrA [H]

Synonym: Shewmr4_1165

Alternate gene names: 113969509

Gene position: 1371647-1373128 (Clockwise)

Preceding gene: 113969508

Following gene: 113969510

Centisome position: 29.14

GC content: 51.96

Gene sequence:

>1482_bases
ATGAGTGATAATCAACCCCAAGCGGCAAAGCACACGTTGATGTGGTTTCGCCAGGATCTGCGCCTAACGGATAACCAAGC
ACTGACGGCAGCCTGTGATTGGGTGCGGCAAGTGCCTGGCGCAAAACTCAGGGCGATTTATATTGCGACTCCGAGCCAAT
GGCTCAGCCACGATATGGCGCCGATCCAGATTGATTTTATCCAGCGGCATGTGAATTTGCTCGCTCAAGGACTCGCAAGC
CTAGGGATTGAATTTGAGCTTATTCAACTTGATAGCTTTAAAGAAGTGCCCGCCTTTTTAGCGAGTTACTGTGAAAAGCT
TGGAGTTGAGCGGGTGTTCGCGGGGCGAGAACCCGAAATCAACGAGCAAAAGCGCGACCAAGCCTGTCTCGATGTGGGTA
TTCCCTTGGTGCTCAGCGACGAGCATTGCCTATTACCGCCGGGCACAGTGCTTAACTTAAGCGGTGATATGTATAAGGTG
TTCACGCCCTTTAGCCGCAAGTGGCGAGAAGTTGCCGCCAAGCGCGCCATTTTGCCGCTTGCTGTACCTGCGCCGCTTGG
CCCTATGTTACCTGAACCAAAGCCAATCAGTTTTAAAGAACGTTTCCCATTCACTAGCAAGCTAACCTCTAGCGAGCAAT
GGGCGGCGGGAGAGGGGCAGGCAAAACGTATTCTTACCGCATTTATTCAGCAAAAAGCCCAAGACTATAAACAGGATAGG
GACTTTCCTGCTCTCGAGGGTACCAGTGTTATCTCCCCTTATTTGGCGCTGGGTGTGCTGAGTCCAAGGCAATGTATTGC
GGCGCTGCTTGGGCGTTTTCCCGAGGTAATTGTCGATGATACTAGCCCTGGGCGAACCTGGTTGAATGAGCTGATCTGGC
GGGAGTTTTATCGCCATTTGTTAGTGGCTTTTCCAGATTTATCCAAGGGGCATAACTTTAATCGTCAGGCCGACCATGTG
CAGTGGCGCAACAACCAAGCTGAGTTTTTGGCATGGTGCGAGGGCAAAACGGGTTACCCGATTGTAGATGCGGCAATGCG
CCAGCTTAATCAAACTGGCTGGATGCACAATCGACTGCGCATGGTGGTGGCGAGCTTTTTAACCAAACACTTGCTTATCG
ATTGGCGCTGGGGTGAACGTTACTTCCGGCAGCATCTTATCGACGGCGATCTGGCGGCTAATAACGGTGGCTGGCAATGG
TCGGCGGGCTGTGGCTGCGATGCCCAGCCTTATTTCCGCATTTTTAATCCTATCAGCCAGAGCGAAAAGTTTGATCCAGA
TGGCAGCTTTATCCGTAAGTACTTACCAGAGCTGGCATCTTGGGGAATCAAGCGTTTGCACCAACCCAATATGCAAAAAA
CGCCTTCGTTATTTGAGGTGCCAACGCTGGAGTCTGACTCGGCTTATCCCTATGCAATTGTGGAGCACAATGCGGCAAGG
GCGAGGGCGCTAGCGGTACTTGGGGTGCTGAAAAAGGCCTAG

Upstream 100 bases:

>100_bases
GCTGAATGGGTTAGCCAAGTTTCGGCGGCAAAAGAGCAACAGAAGAAACAGCTACGTAAGAACAAAAACGCGGCCGAAGC
GGAGCAAGGAGGGCAAGCCG

Downstream 100 bases:

>100_bases
GCCATAGGCTCAACCTTACAACTTATCCCTTAGGGAGTTATCTGGATGATAAACCAAGCGAAGATTGAACAGAGCGGTGT
GGTTGATGAGTTTATCGCGC

Product: deoxyribodipyrimidine photo-lyase type I

Products: NA

Alternate protein names: DNA photolyase; Photoreactivating enzyme [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSDNQPQAAKHTLMWFRQDLRLTDNQALTAACDWVRQVPGAKLRAIYIATPSQWLSHDMAPIQIDFIQRHVNLLAQGLAS
LGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEINEQKRDQACLDVGIPLVLSDEHCLLPPGTVLNLSGDMYKV
FTPFSRKWREVAAKRAILPLAVPAPLGPMLPEPKPISFKERFPFTSKLTSSEQWAAGEGQAKRILTAFIQQKAQDYKQDR
DFPALEGTSVISPYLALGVLSPRQCIAALLGRFPEVIVDDTSPGRTWLNELIWREFYRHLLVAFPDLSKGHNFNRQADHV
QWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQW
SAGCGCDAQPYFRIFNPISQSEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEVPTLESDSAYPYAIVEHNAAR
ARALAVLGVLKKA

Sequences:

>Translated_493_residues
MSDNQPQAAKHTLMWFRQDLRLTDNQALTAACDWVRQVPGAKLRAIYIATPSQWLSHDMAPIQIDFIQRHVNLLAQGLAS
LGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEINEQKRDQACLDVGIPLVLSDEHCLLPPGTVLNLSGDMYKV
FTPFSRKWREVAAKRAILPLAVPAPLGPMLPEPKPISFKERFPFTSKLTSSEQWAAGEGQAKRILTAFIQQKAQDYKQDR
DFPALEGTSVISPYLALGVLSPRQCIAALLGRFPEVIVDDTSPGRTWLNELIWREFYRHLLVAFPDLSKGHNFNRQADHV
QWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQW
SAGCGCDAQPYFRIFNPISQSEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEVPTLESDSAYPYAIVEHNAAR
ARALAVLGVLKKA
>Mature_492_residues
SDNQPQAAKHTLMWFRQDLRLTDNQALTAACDWVRQVPGAKLRAIYIATPSQWLSHDMAPIQIDFIQRHVNLLAQGLASL
GIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEINEQKRDQACLDVGIPLVLSDEHCLLPPGTVLNLSGDMYKVF
TPFSRKWREVAAKRAILPLAVPAPLGPMLPEPKPISFKERFPFTSKLTSSEQWAAGEGQAKRILTAFIQQKAQDYKQDRD
FPALEGTSVISPYLALGVLSPRQCIAALLGRFPEVIVDDTSPGRTWLNELIWREFYRHLLVAFPDLSKGHNFNRQADHVQ
WRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLRMVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQWS
AGCGCDAQPYFRIFNPISQSEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEVPTLESDSAYPYAIVEHNAARA
RALAVLGVLKKA

Specific function: Involved in repair of UV radiation-induced DNA damage. Catalyzes the light-dependent monomerization (300-600 nm) of cyclobutyl pyrimidine dimers (in cis-syn configuration), which are formed between adjacent bases on the same DNA strand upon exposure to ul

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain [H]

Homologues:

Organism=Homo sapiens, GI188536100, Length=488, Percent_Identity=26.844262295082, Blast_Score=153, Evalue=4e-37,
Organism=Homo sapiens, GI4758072, Length=490, Percent_Identity=27.1428571428571, Blast_Score=147, Evalue=2e-35,
Organism=Homo sapiens, GI188536103, Length=392, Percent_Identity=27.2959183673469, Blast_Score=134, Evalue=3e-31,
Organism=Escherichia coli, GI1786926, Length=489, Percent_Identity=47.239263803681, Blast_Score=401, Evalue=1e-113,
Organism=Saccharomyces cerevisiae, GI6324962, Length=505, Percent_Identity=33.4653465346535, Blast_Score=257, Evalue=3e-69,
Organism=Drosophila melanogaster, GI17137248, Length=508, Percent_Identity=27.9527559055118, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24585455, Length=508, Percent_Identity=27.9527559055118, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24648152, Length=528, Percent_Identity=23.6742424242424, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002081
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7 [H]

EC number: =4.1.99.3 [H]

Molecular weight: Translated: 55953; Mature: 55822

Theoretical pI: Translated: 8.21; Mature: 8.21

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1 ; PS00691 DNA_PHOTOLYASES_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNQPQAAKHTLMWFRQDLRLTDNQALTAACDWVRQVPGAKLRAIYIATPSQWLSHDMA
CCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCC
PIQIDFIQRHVNLLAQGLASLGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEI
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHCCCCCCC
NEQKRDQACLDVGIPLVLSDEHCLLPPGTVLNLSGDMYKVFTPFSRKWREVAAKRAILPL
CHHHHHHHHHHCCCCEEECCCCEECCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCEE
AVPAPLGPMLPEPKPISFKERFPFTSKLTSSEQWAAGEGQAKRILTAFIQQKAQDYKQDR
ECCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DFPALEGTSVISPYLALGVLSPRQCIAALLGRFPEVIVDDTSPGRTWLNELIWREFYRHL
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHH
LVAFPDLSKGHNFNRQADHVQWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLR
HHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHH
MVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQWSAGCGCDAQPYFRIFNPISQ
HHHHHHHHHHHEEEEEHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCHHHHHCCHHC
SEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEVPTLESDSAYPYAIVEHNAAR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEEECCHHH
ARALAVLGVLKKA
HHHHHHHHHHHCC
>Mature Secondary Structure 
SDNQPQAAKHTLMWFRQDLRLTDNQALTAACDWVRQVPGAKLRAIYIATPSQWLSHDMA
CCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCEEEEEEEECCHHHHHCCCC
PIQIDFIQRHVNLLAQGLASLGIEFELIQLDSFKEVPAFLASYCEKLGVERVFAGREPEI
CCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCHHHHHCCCCCCC
NEQKRDQACLDVGIPLVLSDEHCLLPPGTVLNLSGDMYKVFTPFSRKWREVAAKRAILPL
CHHHHHHHHHHCCCCEEECCCCEECCCCCEEECCCCEEEECCHHHHHHHHHHHHHCCCEE
AVPAPLGPMLPEPKPISFKERFPFTSKLTSSEQWAAGEGQAKRILTAFIQQKAQDYKQDR
ECCCCCCCCCCCCCCCCHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
DFPALEGTSVISPYLALGVLSPRQCIAALLGRFPEVIVDDTSPGRTWLNELIWREFYRHL
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHH
LVAFPDLSKGHNFNRQADHVQWRNNQAEFLAWCEGKTGYPIVDAAMRQLNQTGWMHNRLR
HHHCCCCCCCCCCCCCCCHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHCHHHHHHH
MVVASFLTKHLLIDWRWGERYFRQHLIDGDLAANNGGWQWSAGCGCDAQPYFRIFNPISQ
HHHHHHHHHHHEEEEEHHHHHHHHHHCCCCEECCCCCEEECCCCCCCCCCHHHHHCCHHC
SEKFDPDGSFIRKYLPELASWGIKRLHQPNMQKTPSLFEVPTLESDSAYPYAIVEHNAAR
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCCCCCCCCCEEEEECCHHH
ARALAVLGVLKKA
HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]