Click here to switch to the map view.

The map label for this gene is thiL [H]

Identifier: 113969445

GI number: 113969445

Start: 1291771

End: 1292727

Strand: Direct

Name: thiL [H]

Synonym: Shewmr4_1101

Alternate gene names: 113969445

Gene position: 1291771-1292727 (Clockwise)

Preceding gene: 113969444

Following gene: 113969446

Centisome position: 27.45

GC content: 52.56

Gene sequence:

>957_bases
GTGAAAGAATTCCAACTTATTGAATGTTATTTCAATCACCGCGGCCCCACGCGCCGTGATGTCAAACTGAGTATTGGCGA
CGATTGCGCCCTAGTGCAGCCCGCCGAAAACAAGTCGATCGCCATCTCCTGCGATACCTTAGTCGAAAACGTTCACTTCT
TCCCCGATATTCCCCCTCAAGCCTTAGGTTACAAAGCCTTAGCAGTGAATCTTTCAGATTTAGCCGCCATGGGGGCAGAG
CCTGCGTGGATGACGCTTGCGCTTACCTTACCAGAGGTTAACGAAGCCTGGCTGAGTGGGTTTAGTGAGGGACTCTTCGA
AGCGGCTGAATACTATGGTATTGCCCTCATTGGCGGCGATACCACCCGCGGCCCTAGAGCCATTAACATTACCGTGCATG
GACAAGTGCCGCAGGGTAAGGCATTGACTCGTCACGGTGCAAAAGCGGGTGACTGGATTTATGTGACCGGTACGCTCGGC
GATTCAGCATTAGGGCTGGATTTGATCCGTGGCGTGCAGCATGCGCGTGCGGAGCATAAAGAGTTCCTAATCAATCGCCA
TTATCGTCCAACACCTCGAGTGTTAGCGGGTCAGTCGTTACGGTCTTTAGCATCCAGCGCCATCGACTTATCCGACGGTT
TTATTTCGGACATTGGCCATATCCTCAAAGCATCGCAGGTAGGCGCGGTTGTGGATGTAGGCTTGATTCCGTTGTCCCGC
GCCATGCAGGATACCGTGAGCGAAGAGCATGCCTTAGGTTATGCGCTAACGGGGGGCGAGGATTATGAGCTGCTCTTTAC
CGTGCCAGAGGCGCAAAAAGGTGCGTTAGAAACTGCACTGAGCCACGCGGGGACTAAGTTTGTGCGGGTTGGACAAATTT
GTGCGGGCAGTAAACTTAAGTTGCAACTCAATGGTGAGCCGTTTACGCCGCCATATCATGGTTTTGAGCACTTTTAA

Upstream 100 bases:

>100_bases
CAAACTGGTTGCACGTAAGTAATAAGGACAAACGGTATCTTAGGATACCGTTTTTCATTGCTAACAAAGGTGGCCGATGT
GAGTCGCGCCAAACTGTACA

Downstream 100 bases:

>100_bases
TGAAGTTGTTATCCCAAGATCAGGCGCTGTCGCGCCTGTCTCTTAAAAATCCAATCCATTTTTTAGCATTAGGCTTTGGC
AGTGGTTTAGCGGCTAAGGC

Product: thiamine-monophosphate kinase

Products: NA

Alternate protein names: Thiamine-phosphate kinase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKEFQLIECYFNHRGPTRRDVKLSIGDDCALVQPAENKSIAISCDTLVENVHFFPDIPPQALGYKALAVNLSDLAAMGAE
PAWMTLALTLPEVNEAWLSGFSEGLFEAAEYYGIALIGGDTTRGPRAINITVHGQVPQGKALTRHGAKAGDWIYVTGTLG
DSALGLDLIRGVQHARAEHKEFLINRHYRPTPRVLAGQSLRSLASSAIDLSDGFISDIGHILKASQVGAVVDVGLIPLSR
AMQDTVSEEHALGYALTGGEDYELLFTVPEAQKGALETALSHAGTKFVRVGQICAGSKLKLQLNGEPFTPPYHGFEHF

Sequences:

>Translated_318_residues
MKEFQLIECYFNHRGPTRRDVKLSIGDDCALVQPAENKSIAISCDTLVENVHFFPDIPPQALGYKALAVNLSDLAAMGAE
PAWMTLALTLPEVNEAWLSGFSEGLFEAAEYYGIALIGGDTTRGPRAINITVHGQVPQGKALTRHGAKAGDWIYVTGTLG
DSALGLDLIRGVQHARAEHKEFLINRHYRPTPRVLAGQSLRSLASSAIDLSDGFISDIGHILKASQVGAVVDVGLIPLSR
AMQDTVSEEHALGYALTGGEDYELLFTVPEAQKGALETALSHAGTKFVRVGQICAGSKLKLQLNGEPFTPPYHGFEHF
>Mature_318_residues
MKEFQLIECYFNHRGPTRRDVKLSIGDDCALVQPAENKSIAISCDTLVENVHFFPDIPPQALGYKALAVNLSDLAAMGAE
PAWMTLALTLPEVNEAWLSGFSEGLFEAAEYYGIALIGGDTTRGPRAINITVHGQVPQGKALTRHGAKAGDWIYVTGTLG
DSALGLDLIRGVQHARAEHKEFLINRHYRPTPRVLAGQSLRSLASSAIDLSDGFISDIGHILKASQVGAVVDVGLIPLSR
AMQDTVSEEHALGYALTGGEDYELLFTVPEAQKGALETALSHAGTKFVRVGQICAGSKLKLQLNGEPFTPPYHGFEHF

Specific function: Thiamine biosynthesis. [C]

COG id: COG0611

COG function: function code H; Thiamine monophosphate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiamine-monophosphate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1786619, Length=318, Percent_Identity=58.1761006289308, Blast_Score=369, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR016188
- InterPro:   IPR006283 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: =2.7.4.16 [H]

Molecular weight: Translated: 34288; Mature: 34288

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFQLIECYFNHRGPTRRDVKLSIGDDCALVQPAENKSIAISCDTLVENVHFFPDIPPQ
CCCCEEEEEEECCCCCCCCEEEEEECCCEEEECCCCCCEEEEEHHHHHHCCCCCCCCCHH
ALGYKALAVNLSDLAAMGAEPAWMTLALTLPEVNEAWLSGFSEGLFEAAEYYGIALIGGD
HHCCEEEEEEHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCC
TTRGPRAINITVHGQVPQGKALTRHGAKAGDWIYVTGTLGDSALGLDLIRGVQHARAEHK
CCCCCEEEEEEEECCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
EFLINRHYRPTPRVLAGQSLRSLASSAIDLSDGFISDIGHILKASQVGAVVDVGLIPLSR
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHH
AMQDTVSEEHALGYALTGGEDYELLFTVPEAQKGALETALSHAGTKFVRVGQICAGSKLK
HHHHHHHHHHCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCEECCCEEE
LQLNGEPFTPPYHGFEHF
EEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MKEFQLIECYFNHRGPTRRDVKLSIGDDCALVQPAENKSIAISCDTLVENVHFFPDIPPQ
CCCCEEEEEEECCCCCCCCEEEEEECCCEEEECCCCCCEEEEEHHHHHHCCCCCCCCCHH
ALGYKALAVNLSDLAAMGAEPAWMTLALTLPEVNEAWLSGFSEGLFEAAEYYGIALIGGD
HHCCEEEEEEHHHHHHCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHEEEEEECCC
TTRGPRAINITVHGQVPQGKALTRHGAKAGDWIYVTGTLGDSALGLDLIRGVQHARAEHK
CCCCCEEEEEEEECCCCCCCHHHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
EFLINRHYRPTPRVLAGQSLRSLASSAIDLSDGFISDIGHILKASQVGAVVDVGLIPLSR
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCHHHHH
AMQDTVSEEHALGYALTGGEDYELLFTVPEAQKGALETALSHAGTKFVRVGQICAGSKLK
HHHHHHHHHHCCEEEEECCCCEEEEEECCCCCHHHHHHHHHHCCCCEEEECCEECCCEEE
LQLNGEPFTPPYHGFEHF
EEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]