Click here to switch to the map view.

The map label for this gene is glyA [H]

Identifier: 113969438

GI number: 113969438

Start: 1285675

End: 1286928

Strand: Direct

Name: glyA [H]

Synonym: Shewmr4_1094

Alternate gene names: 113969438

Gene position: 1285675-1286928 (Clockwise)

Preceding gene: 113969436

Following gene: 113969439

Centisome position: 27.32

GC content: 48.96

Gene sequence:

>1254_bases
ATGCTGAAAAAAGATATGAATATCGCAGATTATGATCCGGAACTGTTCAACGCAATTCAGAACGAAACTCTGCGTCAAGA
AGAGCATATTGAGCTGATTGCTTCTGAAAACTACACCAGTCCACGCGTGATGCAAGCGCAAGGTTCACAATTAACCAACA
AGTACGCCGAAGGTTATCCTGGCAAGCGTTACTACGGTGGTTGTGAGTATGTGGACGTAGTTGAAACCTTAGCAATTGAG
CGTGCAAAACAACTGTTTGGTGCGACTTACGCAAACGTGCAACCTCACTCAGGTTCTCAAGCAAACAGCGCCGTTTACAT
GGCATTGTTAAAACCAGGCGATACCGTTTTAGGTATGAACCTCGCTCACGGTGGTCACTTGACCCACGGTTCACCTGTAA
ACTTCTCCGGTAGACTGTACAACATCATTCCTTACGGCATCGATGAATCAGGCAAAATCGACTACGACGAAATGGAACGT
CTGGCAGTTGAACATAAGCCTAAGATGATGATCGGTGGTTTCTCTGCTTACTCAGGCATCGTTGACTGGGCAAGAATGCG
CGAAATCGCAGACAAAATCGGTGCTTACCTGTTTGTCGACATGGCGCACGTTGCGGGTCTTATCGCCGCTGGTGTGTATC
CAAACCCTGTGCCACACGCGCACGTTGTGACCTCAACCACTCACAAGACCTTAGCCGGTCCTCGTGGCGGTATCATTCTG
TCTGCAGCCGATGATGAAGATTTATACAAAAAGCTGAACTCTGCGGTATTCCCAGGCGGTCAAGGCGGTCCTTTGATGCA
CGTTATCGCAGGTAAAGCGGTAGCCTTCAAAGAAGCTTTAGAGCCAGAGTTCAAAGCTTACCAACAACAAGTGGTTAAGA
ACGCTAAAGCTATGGTTGAAGTGTTCTTAGAGCGCGGTTACAAGATCGTTTCTGGCGGTACTGACAACCACTTAATGCTG
GTGGACTTAATCGGTCGCGACCTGACGGGTAAAGAAGCCGATGCCGCTTTAGGTAGCGCGAACATCACAGTAAACAAAAA
CTCTGTGCCTAACGATCCACGTTCTCCATTCGTGACCTCTGGTGTGCGTATTGGTACGCCTGCGATCACTCGCCGCGGCT
TTAAAGAAGCTGAAGCGAAAGAGTTAACCGGTTGGATCTGTGACATCCTCGACGATGCCCACAACCCAGCAGTCATCGAG
CGCGTAAAAGGCCAAGTATTGGCACTGTGCGCCCGTTTCCCTGTTTACGGTTAA

Upstream 100 bases:

>100_bases
AACTGTAGGATTTTCATGTTTCAGAGACTGGGATTGTCGGCTCGTTACGAGTAGAATACCGCGCAACCCTACCTACAAGA
ATTGGCAGCTGGAGTAAGCA

Downstream 100 bases:

>100_bases
TTCGTTAATTTATCTGAATATGAAGGCTGAGTTCACAACTGCTTAGGGTGTTACTCTTAGGTGGTTAACCTCAGCCGATA
AATAGGATAAAATCCCATGG

Product: serine hydroxymethyltransferase

Products: NA

Alternate protein names: SHMT; Serine methylase [H]

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGRLYNIIPYGIDESGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWARMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKAYQQQVVKNAKAMVEVFLERGYKIVSGGTDNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEAEAKELTGWICDILDDAHNPAVIE
RVKGQVLALCARFPVYG

Sequences:

>Translated_417_residues
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGRLYNIIPYGIDESGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWARMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKAYQQQVVKNAKAMVEVFLERGYKIVSGGTDNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEAEAKELTGWICDILDDAHNPAVIE
RVKGQVLALCARFPVYG
>Mature_417_residues
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYPGKRYYGGCEYVDVVETLAIE
RAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMNLAHGGHLTHGSPVNFSGRLYNIIPYGIDESGKIDYDEMER
LAVEHKPKMMIGGFSAYSGIVDWARMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKAYQQQVVKNAKAMVEVFLERGYKIVSGGTDNHLML
VDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTSGVRIGTPAITRRGFKEAEAKELTGWICDILDDAHNPAVIE
RVKGQVLALCARFPVYG

Specific function: Interconversion of serine and glycine [H]

COG id: COG0112

COG function: function code E; Glycine/serine hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SHMT family [H]

Homologues:

Organism=Homo sapiens, GI19923315, Length=402, Percent_Identity=46.2686567164179, Blast_Score=355, Evalue=4e-98,
Organism=Homo sapiens, GI261862352, Length=402, Percent_Identity=46.2686567164179, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862350, Length=402, Percent_Identity=46.2686567164179, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862348, Length=402, Percent_Identity=46.2686567164179, Blast_Score=355, Evalue=5e-98,
Organism=Homo sapiens, GI261862346, Length=401, Percent_Identity=45.8852867830424, Blast_Score=343, Evalue=2e-94,
Organism=Homo sapiens, GI22547186, Length=401, Percent_Identity=47.3815461346633, Blast_Score=335, Evalue=5e-92,
Organism=Homo sapiens, GI22547189, Length=389, Percent_Identity=45.2442159383033, Blast_Score=305, Evalue=6e-83,
Organism=Escherichia coli, GI1788902, Length=416, Percent_Identity=83.4134615384615, Blast_Score=722, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25144732, Length=401, Percent_Identity=46.1346633416459, Blast_Score=346, Evalue=1e-95,
Organism=Caenorhabditis elegans, GI25144729, Length=401, Percent_Identity=46.1346633416459, Blast_Score=345, Evalue=2e-95,
Organism=Saccharomyces cerevisiae, GI6319739, Length=415, Percent_Identity=44.8192771084337, Blast_Score=364, Evalue=1e-101,
Organism=Saccharomyces cerevisiae, GI6323087, Length=402, Percent_Identity=46.0199004975124, Blast_Score=345, Evalue=7e-96,
Organism=Drosophila melanogaster, GI24640005, Length=400, Percent_Identity=47.5, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI221329721, Length=400, Percent_Identity=47.5, Blast_Score=368, Evalue=1e-102,

Paralogues:

None

Copy number: 3180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 12,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR001085
- InterPro:   IPR019798 [H]

Pfam domain/function: PF00464 SHMT [H]

EC number: =2.1.2.1 [H]

Molecular weight: Translated: 45282; Mature: 45282

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00096 SHMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCCEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDVVETLAIERAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGRLYNIIPYGIDESGKIDYDEMERLAVEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKAYQQQVVKNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTDNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEC
GVRIGTPAITRRGFKEAEAKELTGWICDILDDAHNPAVIERVKGQVLALCARFPVYG
CEEECCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLKKDMNIADYDPELFNAIQNETLRQEEHIELIASENYTSPRVMQAQGSQLTNKYAEGYP
CCCCCCCCCCCCHHHHHHHHHHHHCCHHHEEEEECCCCCCCCEECCCCHHHHHHHHCCCC
GKRYYGGCEYVDVVETLAIERAKQLFGATYANVQPHSGSQANSAVYMALLKPGDTVLGMN
CCCCCCCCHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCCEEEEEEECCCCEEEEEE
LAHGGHLTHGSPVNFSGRLYNIIPYGIDESGKIDYDEMERLAVEHKPKMMIGGFSAYSGI
ECCCCCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEECHHHHHHH
VDWARMREIADKIGAYLFVDMAHVAGLIAAGVYPNPVPHAHVVTSTTHKTLAGPRGGIIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHCCCCCCEEE
SAADDEDLYKKLNSAVFPGGQGGPLMHVIAGKAVAFKEALEPEFKAYQQQVVKNAKAMVE
EECCCHHHHHHHHHCCCCCCCCCCEEEEHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHH
VFLERGYKIVSGGTDNHLMLVDLIGRDLTGKEADAALGSANITVNKNSVPNDPRSPFVTS
HHHHCCCEEEECCCCCCEEEEEECCCCCCCCCHHHHCCCCEEEECCCCCCCCCCCCCEEC
GVRIGTPAITRRGFKEAEAKELTGWICDILDDAHNPAVIERVKGQVLALCARFPVYG
CEEECCHHHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA