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The map label for this gene is carA [H]

Identifier: 113969309

GI number: 113969309

Start: 1128808

End: 1129968

Strand: Direct

Name: carA [H]

Synonym: Shewmr4_0965

Alternate gene names: 113969309

Gene position: 1128808-1129968 (Clockwise)

Preceding gene: 113969308

Following gene: 113969310

Centisome position: 23.99

GC content: 47.8

Gene sequence:

>1161_bases
ATGGAGGTCGCGTTGACAAAGTCTGCCTTACTCGTACTCGAAGATGGAACCGTATTCACTGGCACAGCGATAGGTGCCGA
TGGTCTCTCTGTTGGTGAAGTTGTTTTTAACACTTCGATGACTGGATACCAAGAAATTTTAACTGATCCCTCTTATTCAC
GCCAGATTGTAACTTTAACCTACCCCCACATTGGTAATACTGGTACCAATAATGAAGACGTCGAATCCAATGGGGTTCAT
GCTTGTGGTTTAATCATTCGCGATCTTCCGCTTATAGCAAGTAGTTTCCGTAATCAACAAACTCTCAGCGATTATCTCAA
AGCAAACAATGTTGTTGGCATAGCAGATATCGATACTCGTAAATTAACTCGAATCCTCCGTGAAAAAGGTGCCCAAGCGG
GTTGCATCATGGCGGGTGCTGACTTAGATGAAGCCAAAGCACTTGCTGCTGCTAAAGCCTTCCCGGGTTTAAAGGGCATG
GATTTAGCGAAAGAAGTGACTACCGAGACGGCCTATCAATGGCGTAAAGGTAGTTGGCGTTTGGTGGGCGGTTTGCCTGA
AGACACGCCAGCTGAAGCGCTGAAGTACAAAGTGGTTGCCTATGACTACGGCGTTAAGCAAAACATCCTGCGCATGTTGG
TTGACCGTGGCTGTGATGTCACAGTGGTTCCTGCAAAAACATCGGCTGCTGAAGTGTTAGCGATGAACCCAGACGGTGTG
TTCCTCTCTAACGGCCCTGGTGACCCAGAGCCATGTGATTACGCCATCGAAGCGATTCAACAAATCCTGAAAACCGATAC
GCCGATTTTTGGTATCTGTTTAGGCCACCAATTACTGGCATTGGCATCAGGCGCGAAAACCTTAAAGATGAAGTTTGGTC
ACCACGGTGCTAACCACCCAGTGAGCAATATCGAAAAAGGTAACGTGATGATCACCAGCCAAAACCACGGTTTTGCTGCC
GATGAAGCCACATTACCTGCCAATATCAAAGTGACCCACAAGTCACTGTTCGATGGTTCACTGCAAGGTATCCATCTGAC
GGACAAACCTGCGTTCAGCTTCCAAGGTCACCCTGAAGCGAGCCCTGGTCCGAACGATGCGGCCCCACTGTTCGATCACT
TTATCGAGCTTATTGAACAATACCGTCAGAACGCTAAGTAA

Upstream 100 bases:

>100_bases
TCCTGTAAACTCGCTGGAATTTGTCAAAATTTCGTAAATCAGCGGAAATTGGTATCTTAATACAACATAAAAACATTATA
TTTCAGTGGCTTGGCTCTTT

Downstream 100 bases:

>100_bases
TACGCAAGCTAGGAGAAGAGAGAAGCAATGCCAAAACGTACAGATATAAAGAGTATTCTTATCCTAGGTGCAGGCCCGAT
TGTCATTGGTCAGGCGTGTG

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 386; Mature: 386

Protein sequence:

>386_residues
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDVESNGVH
ACGLIIRDLPLIASSFRNQQTLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTETAYQWRKGSWRLVGGLPEDTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAA
DEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIEQYRQNAK

Sequences:

>Translated_386_residues
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDVESNGVH
ACGLIIRDLPLIASSFRNQQTLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTETAYQWRKGSWRLVGGLPEDTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAA
DEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIEQYRQNAK
>Mature_386_residues
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLTYPHIGNTGTNNEDVESNGVH
ACGLIIRDLPLIASSFRNQQTLSDYLKANNVVGIADIDTRKLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGM
DLAKEVTTETAYQWRKGSWRLVGGLPEDTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHPVSNIEKGNVMITSQNHGFAA
DEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEASPGPNDAAPLFDHFIELIEQYRQNAK

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=383, Percent_Identity=38.3812010443864, Blast_Score=245, Evalue=5e-65,
Organism=Homo sapiens, GI21361331, Length=387, Percent_Identity=36.4341085271318, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI169790915, Length=387, Percent_Identity=36.4341085271318, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI1786215, Length=382, Percent_Identity=77.2251308900524, Blast_Score=622, Evalue=1e-179,
Organism=Caenorhabditis elegans, GI193204318, Length=385, Percent_Identity=37.1428571428571, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6324878, Length=397, Percent_Identity=39.0428211586902, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6322331, Length=410, Percent_Identity=34.8780487804878, Blast_Score=231, Evalue=1e-61,
Organism=Drosophila melanogaster, GI45555749, Length=389, Percent_Identity=39.5886889460154, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24642586, Length=389, Percent_Identity=39.5886889460154, Blast_Score=235, Evalue=3e-62,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 41347; Mature: 41347

Theoretical pI: Translated: 5.57; Mature: 5.57

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEEECEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNNEDVESNGVHACGLIIRDLPLIASSFRNQQTLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGMDLAKEVTTETAYQWRKGSWR
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEE
LVGGLPEDTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
EECCCCCCCCHHHHEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSNIEKGNVMITSQNHGFAADEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIELIEQYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEVALTKSALLVLEDGTVFTGTAIGADGLSVGEVVFNTSMTGYQEILTDPSYSRQIVTLT
CCEEEECCEEEEEECCCEEEECEECCCCCCHHHHEECCCCCHHHHHHCCCCCCCEEEEEE
YPHIGNTGTNNEDVESNGVHACGLIIRDLPLIASSFRNQQTLSDYLKANNVVGIADIDTR
CCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHCCHHHHHHHHHHCCEEEEECCCHH
KLTRILREKGAQAGCIMAGADLDEAKALAAAKAFPGLKGMDLAKEVTTETAYQWRKGSWR
HHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEE
LVGGLPEDTPAEALKYKVVAYDYGVKQNILRMLVDRGCDVTVVPAKTSAAEVLAMNPDGV
EECCCCCCCCHHHHEEEEEEEECCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEECCCCE
FLSNGPGDPEPCDYAIEAIQQILKTDTPIFGICLGHQLLALASGAKTLKMKFGHHGANHP
EECCCCCCCCHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCEEEEEECCCCCCCC
VSNIEKGNVMITSQNHGFAADEATLPANIKVTHKSLFDGSLQGIHLTDKPAFSFQGHPEA
CCCCCCCCEEEEECCCCCCCCCCCCCCCEEEEEHHHCCCCCCEEEECCCCCCCCCCCCCC
SPGPNDAAPLFDHFIELIEQYRQNAK
CCCCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]