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The map label for this gene is hprA [H]

Identifier: 113969257

GI number: 113969257

Start: 1064049

End: 1065002

Strand: Direct

Name: hprA [H]

Synonym: Shewmr4_0913

Alternate gene names: 113969257

Gene position: 1064049-1065002 (Clockwise)

Preceding gene: 113969256

Following gene: 113969260

Centisome position: 22.61

GC content: 53.35

Gene sequence:

>954_bases
ATGAAAATAGTCGTACTCGATGGTGAAACCTTAAACCCTGGGGATTTAACTTGGCAAGCGGTCAGCGCATTAGGGGAATT
TAGCTGTTTTGCCCGCACGCCCTCGGCGGAGATTATCCCGCGCGCTCAGGATGCCGAGATAGTGCTCACCAACAAGACGC
CCTTCGATGCCAACACCTTAGCCCAACTGCCTAAGCTTAAATATATCGGCGTGCTTGCCACGGGCACCAACGTGGTGGAT
CTTGCCGCCGCAAAAGAGTTAGGCATAGTGGTCACCAACGTGCCCGCCTATGGCCCCGATGCCGTCGCGCAAATGGTGTT
TGCCCATATATTGCATCATACGCAAGCCGTTGCCGCGCACCATCAAGCCGTTGCCGCAGGGCAATGGAGCAATTGCAGTG
ACTTTTGTTTTACCTTAATGCCGCTGCAATCCCTTAAAGGCAAAACCTTAGGCTTGATTGGTTATGGGGATATTGGCCAG
CAGGTGGCTAAACTCGCCTTAGCCTTTGGCATGAAGGTATTAGTGAATACCCGCACCGAACCTAGCGATTTACCCCTAGG
CGTGAGTTGGACATCCCGCGATACGGTATTTAAGGAGTCCGATATTCTCTCGCTCCACTGCCCACTCACCCTTGAGACCA
CCGAGCTGATTAATGCCCAAACCCTCGAATTAATGAAGCCGCAGGCGTTACTGATCAACACCGCGCGCGGCGGCTTAATC
GATGAGGCCGCGCTTGCGGCCGCATTAACGCAGGGCAAAGTCTTTGCTGGCGTCGATGTACTCTCGACCGAACCACCGAG
TGCAGATAATCCGCTACTAACAGCGCCCAATATCAGTATCAGCCCCCACAATGCTTGGGCGACCAAAGAAGCGCGGCAGA
ACCTACTCAATATCGCGACGGCAAATCTCAGCGCTTATTTAGCTGGCGATAGAGTGAATTGGGTGAATCCCTAA

Upstream 100 bases:

>100_bases
ATCGGATGTCATGTGGCAAGTGCAAGCCAATCTTGGGGTCAAACTCAGTTTTTAATTTCCCTGCTTTTGGGCAAGGTGCA
AACGCAGTTAAGAGGTAAAG

Downstream 100 bases:

>100_bases
GGCTTGTGGGTTAGCTTGTTCCTGTTTAAAGGGAATAGGCTGCTACTCCAGCGTAAATTATGCAGGGATCGTAGGTAGTT
AAAAATGTTGGCACCAACTG

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 317; Mature: 317

Protein sequence:

>317_residues
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQDAEIVLTNKTPFDANTLAQLPKLKYIGVLATGTNVVD
LAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAHHQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVAKLALAFGMKVLVNTRTEPSDLPLGVSWTSRDTVFKESDILSLHCPLTLETTELINAQTLELMKPQALLINTARGGLI
DEAALAAALTQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIATANLSAYLAGDRVNWVNP

Sequences:

>Translated_317_residues
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQDAEIVLTNKTPFDANTLAQLPKLKYIGVLATGTNVVD
LAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAHHQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVAKLALAFGMKVLVNTRTEPSDLPLGVSWTSRDTVFKESDILSLHCPLTLETTELINAQTLELMKPQALLINTARGGLI
DEAALAAALTQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIATANLSAYLAGDRVNWVNP
>Mature_317_residues
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQDAEIVLTNKTPFDANTLAQLPKLKYIGVLATGTNVVD
LAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAHHQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQ
QVAKLALAFGMKVLVNTRTEPSDLPLGVSWTSRDTVFKESDILSLHCPLTLETTELINAQTLELMKPQALLINTARGGLI
DEAALAAALTQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIATANLSAYLAGDRVNWVNP

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=257, Percent_Identity=35.408560311284, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI4557499, Length=257, Percent_Identity=35.408560311284, Blast_Score=135, Evalue=4e-32,
Organism=Homo sapiens, GI61743967, Length=257, Percent_Identity=35.0194552529183, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557497, Length=257, Percent_Identity=35.0194552529183, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI145580575, Length=257, Percent_Identity=35.408560311284, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI23308577, Length=260, Percent_Identity=32.3076923076923, Blast_Score=119, Evalue=5e-27,
Organism=Homo sapiens, GI6912396, Length=249, Percent_Identity=30.1204819277108, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1787645, Length=270, Percent_Identity=35.5555555555556, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI87082289, Length=281, Percent_Identity=33.8078291814947, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI1789279, Length=263, Percent_Identity=34.2205323193916, Blast_Score=121, Evalue=7e-29,
Organism=Escherichia coli, GI87081824, Length=177, Percent_Identity=31.0734463276836, Blast_Score=66, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25147481, Length=268, Percent_Identity=30.2238805970149, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17532191, Length=286, Percent_Identity=29.3706293706294, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6320925, Length=299, Percent_Identity=32.4414715719064, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6322116, Length=299, Percent_Identity=31.7725752508361, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6324055, Length=262, Percent_Identity=30.1526717557252, Blast_Score=105, Evalue=8e-24,
Organism=Saccharomyces cerevisiae, GI6324964, Length=303, Percent_Identity=29.7029702970297, Blast_Score=99, Evalue=8e-22,
Organism=Saccharomyces cerevisiae, GI6321253, Length=182, Percent_Identity=29.6703296703297, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24646446, Length=258, Percent_Identity=35.2713178294574, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646448, Length=258, Percent_Identity=35.2713178294574, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646452, Length=258, Percent_Identity=35.2713178294574, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24646450, Length=258, Percent_Identity=35.2713178294574, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28571528, Length=253, Percent_Identity=33.9920948616601, Blast_Score=131, Evalue=5e-31,
Organism=Drosophila melanogaster, GI62472511, Length=258, Percent_Identity=35.2713178294574, Blast_Score=131, Evalue=6e-31,
Organism=Drosophila melanogaster, GI28574286, Length=252, Percent_Identity=31.3492063492063, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45552429, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=7e-27,
Organism=Drosophila melanogaster, GI28574284, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24585514, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI28574282, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI45551003, Length=282, Percent_Identity=30.1418439716312, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24585516, Length=241, Percent_Identity=30.2904564315353, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19921140, Length=255, Percent_Identity=30.5882352941176, Blast_Score=98, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 33650; Mature: 33650

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQDAEIVLTNKTPFDANTL
CEEEEECCCCCCCCCCHHHHHHHHHHHHHEECCCCHHCCCCCCCCEEEEECCCCCCHHHH
AQLPKLKYIGVLATGTNVVDLAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAH
HHCCCEEEEEEEECCCCEEEEHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQQVAKLALAFGMKVLVNTRTE
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
PSDLPLGVSWTSRDTVFKESDILSLHCPLTLETTELINAQTLELMKPQALLINTARGGLI
CCCCCEEEEECCCCCEEECCCEEEEECCEEECHHHHCCHHHHHHCCCCEEEEECCCCCCH
DEAALAAALTQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIAT
HHHHHHHHHHCCCEEECEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANLSAYLAGDRVNWVNP
CCEEEEEECCEECCCCC
>Mature Secondary Structure
MKIVVLDGETLNPGDLTWQAVSALGEFSCFARTPSAEIIPRAQDAEIVLTNKTPFDANTL
CEEEEECCCCCCCCCCHHHHHHHHHHHHHEECCCCHHCCCCCCCCEEEEECCCCCCHHHH
AQLPKLKYIGVLATGTNVVDLAAAKELGIVVTNVPAYGPDAVAQMVFAHILHHTQAVAAH
HHCCCEEEEEEEECCCCEEEEHHHHHCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
HQAVAAGQWSNCSDFCFTLMPLQSLKGKTLGLIGYGDIGQQVAKLALAFGMKVLVNTRTE
HHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCEEEEECCCC
PSDLPLGVSWTSRDTVFKESDILSLHCPLTLETTELINAQTLELMKPQALLINTARGGLI
CCCCCEEEEECCCCCEEECCCEEEEECCEEECHHHHCCHHHHHHCCCCEEEEECCCCCCH
DEAALAAALTQGKVFAGVDVLSTEPPSADNPLLTAPNISISPHNAWATKEARQNLLNIAT
HHHHHHHHHHCCCEEECEEEECCCCCCCCCCEEECCCCEECCCCCHHHHHHHHHHHHHHH
ANLSAYLAGDRVNWVNP
CCEEEEEECCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]