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The map label for this gene is rumB [H]

Identifier: 113969161

GI number: 113969161

Start: 951534

End: 952688

Strand: Direct

Name: rumB [H]

Synonym: Shewmr4_0817

Alternate gene names: 113969161

Gene position: 951534-952688 (Clockwise)

Preceding gene: 113969160

Following gene: 113969166

Centisome position: 20.22

GC content: 51.86

Gene sequence:

>1155_bases
GTGAGTACATTAGTACAATGTGGGTATTTTGAACGAGGCCAATGCCAATCCTGTCGCCATATTAAGCTCCCTATGGCGCA
GCAACTAGCGGCCAAAACCCAAGAATTGCAGCAATTGTTAGCGCCTTTTGTCGATAACGCCGAACCTCAGTTTTTACCTC
CGGTTGTGGGTGACAGCAGCGGTTTTCGTAACAAGGCGAAAATGGTCGCCTTGGGCGCGGCCCATGCCCCTGTACTGGGG
ATTGTCAGCCCCAGCGGCGAGGCGGTGAGTCTGTGTGACTGTCTGTTGTATCCTGCTGATATGCAAGCCTTACTCCACCG
TTTAGAGCGCTTTGTGCAGCAGGCGGGTATTCCGCCTTACCGTGTCGATAAGGCCAAGGGCGAGCTTAAATTTATCCTAC
TGACGCGCAGCCAAGTGCGCGGCGAGTATATGCTACGTTTCGTGCTGCGCTCGCGGGATGCCATTGCCCGTATCGAACGT
GAATTACCGGCGCTGATGGCCGAATATCCGCAGATCAAAGTCGTCTCAGTCAATTTGCAACCTGTTCATATGGCTATCTT
GGAAGGTGAGGAGGAGATTTTCCTGACCGAAAACACTCGCCTCGAAGAGCGCTTTAACGATGTGCCCTTGTTTATTCGCC
CCAAGAGCTTTTTTCAAACCAACCCGCAGGTGGCGGCAAAGCTGTATCAAACCGCCCGTGAGTGGGTGGCCGACTTCGCA
CCAGCTTCCCTTTGGGACTTATTTTGTGGTGTGGGTGGCTTTGGTTTGCACTGTGCAGCTAAAGATATCCCATTGACAGG
GATTGAAATTGAAGCCGAAGCCATTGCCTGCGCTAAGATGTCGGCGCAGTTGATGGGGCTGGATAAGGTGCAGTTTATGG
CGCTCGACTCCACTGACTTTGCCAAGGGCGATGCGGCGCAAACTAAGCCCGAGTTGATTATTGTCAACCCGCCGCGCCGT
GGGATCGGAGAGTCTCTGTGCCATTCCTTAAGTGAGTTTGCACCTAAGGCGATTTTGTATTCCAGCTGCAATCCTAAAAC
CTTAGCCAAAGATCTCGGCCATATTCGTGGCTATCGGTTAACTAAGGTGCAGTTGTTCGATCTCTTCCCCCACTCGGATC
ATTTCGAAGTATTAGCCTTATTAGTGAAAGACTAA

Upstream 100 bases:

>100_bases
CTGAGGCCTTAATCCTGTTTGTTATGCTTCAGTTGTCATCCAAATTTCACTCGCCATCTCAATAGATAAGATAGACGGGC
ACTGCTGTGGGTACGATGCT

Downstream 100 bases:

>100_bases
ACCTCAACACGCCATTACTCGGCGTCAGTTTCGGCTTTAGGCGTTAAGGCATCGTCCCAGATAATGCTGATTTCGGCGCC
GCCTAAGGCCTGCGAGCGAC

Product: 23S rRNA methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U747)-methyltransferase [H]

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MSTLVQCGYFERGQCQSCRHIKLPMAQQLAAKTQELQQLLAPFVDNAEPQFLPPVVGDSSGFRNKAKMVALGAAHAPVLG
IVSPSGEAVSLCDCLLYPADMQALLHRLERFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIER
ELPALMAEYPQIKVVSVNLQPVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVQFMALDSTDFAKGDAAQTKPELIIVNPPRR
GIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRLTKVQLFDLFPHSDHFEVLALLVKD

Sequences:

>Translated_384_residues
MSTLVQCGYFERGQCQSCRHIKLPMAQQLAAKTQELQQLLAPFVDNAEPQFLPPVVGDSSGFRNKAKMVALGAAHAPVLG
IVSPSGEAVSLCDCLLYPADMQALLHRLERFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIER
ELPALMAEYPQIKVVSVNLQPVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVQFMALDSTDFAKGDAAQTKPELIIVNPPRR
GIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRLTKVQLFDLFPHSDHFEVLALLVKD
>Mature_383_residues
STLVQCGYFERGQCQSCRHIKLPMAQQLAAKTQELQQLLAPFVDNAEPQFLPPVVGDSSGFRNKAKMVALGAAHAPVLGI
VSPSGEAVSLCDCLLYPADMQALLHRLERFVQQAGIPPYRVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIERE
LPALMAEYPQIKVVSVNLQPVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFAP
ASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVQFMALDSTDFAKGDAAQTKPELIIVNPPRRG
IGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRLTKVQLFDLFPHSDHFEVLALLVKD

Specific function: Catalyzes the formation of 5-methyl-uridine at position 747 (M-5-U747) in 23S rRNA [H]

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. RumB subfamily [H]

Homologues:

Organism=Homo sapiens, GI34222389, Length=183, Percent_Identity=28.4153005464481, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI51173878, Length=183, Percent_Identity=28.4153005464481, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1787083, Length=381, Percent_Identity=55.6430446194226, Blast_Score=435, Evalue=1e-123,
Organism=Escherichia coli, GI1789148, Length=182, Percent_Identity=30.7692307692308, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI1790403, Length=185, Percent_Identity=29.1891891891892, Blast_Score=76, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24666579, Length=422, Percent_Identity=25.1184834123223, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011825
- InterPro:   IPR010280 [H]

Pfam domain/function: PF05958 tRNA_U5-meth_tr [H]

EC number: =2.1.1.189 [H]

Molecular weight: Translated: 42641; Mature: 42510

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS01230 TRMA_1 ; PS01231 TRMA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
5.2 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTLVQCGYFERGQCQSCRHIKLPMAQQLAAKTQELQQLLAPFVDNAEPQFLPPVVGDSS
CCCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFRNKAKMVALGAAHAPVLGIVSPSGEAVSLCDCLLYPADMQALLHRLERFVQQAGIPPY
CCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
RVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIERELPALMAEYPQIKVVSVNLQ
EECCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
PVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
EEEEEEECCCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCC
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVQFMALDSTDF
CHHHHHHHHCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AKGDAAQTKPELIIVNPPRRGIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRL
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEE
TKVQLFDLFPHSDHFEVLALLVKD
EEEEEEECCCCCCCHHHHHEEECC
>Mature Secondary Structure 
STLVQCGYFERGQCQSCRHIKLPMAQQLAAKTQELQQLLAPFVDNAEPQFLPPVVGDSS
CCCEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
GFRNKAKMVALGAAHAPVLGIVSPSGEAVSLCDCLLYPADMQALLHRLERFVQQAGIPPY
CCCCCCEEEEEECCCCCEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCE
RVDKAKGELKFILLTRSQVRGEYMLRFVLRSRDAIARIERELPALMAEYPQIKVVSVNLQ
EECCCCCCEEEEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEECC
PVHMAILEGEEEIFLTENTRLEERFNDVPLFIRPKSFFQTNPQVAAKLYQTAREWVADFA
EEEEEEECCCCEEEEECCCCHHHHHCCCCEEECCHHHHCCCHHHHHHHHHHHHHHHHHCC
PASLWDLFCGVGGFGLHCAAKDIPLTGIEIEAEAIACAKMSAQLMGLDKVQFMALDSTDF
CHHHHHHHHCCCCCCEEEECCCCCCCCEEEEHHHHHHHHHHHHHHCCCCEEEEEECCCCC
AKGDAAQTKPELIIVNPPRRGIGESLCHSLSEFAPKAILYSSCNPKTLAKDLGHIRGYRL
CCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCEEEECCCCHHHHHHHHHHHCCCEE
TKVQLFDLFPHSDHFEVLALLVKD
EEEEEEECCCCCCCHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA