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The map label for this gene is nqrF [H]

Identifier: 113969097

GI number: 113969097

Start: 875251

End: 876468

Strand: Direct

Name: nqrF [H]

Synonym: Shewmr4_0753

Alternate gene names: 113969097

Gene position: 875251-876468 (Clockwise)

Preceding gene: 113969096

Following gene: 113969099

Centisome position: 18.6

GC content: 49.59

Gene sequence:

>1218_bases
ATGGAAATGGCAATAGGCATAGGCATGTTCACCTTAGTGGTGTGCCTACTGGTGATAGTAATCCTTATCGCCAAAAAGAA
ATTAGTGACTACCGGTGAGATCACTATCGGGATTAACGACGATGCCGAGAAAAGCATTAAAGTTGCCGCGGGCGACAAAC
TGCTTGGCGCCTTGGCGAGTAAGAATATTTTTATTCCCTCGGCCTGTGGCGGCGGCGGTACCTGCGGCCAGTGCCGGGTG
AAAGTCAAATCGGGCGGTGGTGATATTCTGCCAACAGAACAAGGGCATATCACTAAAAAAGAAGCCAAAGAAGGTTGTCG
TCTCGCCTGCCAAGTCGCGGTGAAAACCGATATGGAACTTGAGCTAGAGGAAGAAATATTCGGTGTGAAGAAGTGGCAAT
GTGAGGTGATCTCAAACGATAACAAGGCCACCTTTATCAAAGAGTTATTGCTAAAACTGCCCGAGGGTGAAGACGTACAC
TTCAAGGCGGGTGGCTATATTCAAATTGAAGCGCCAGCCCATGTGGTGAAGTATGCCGATTTTGATATTCCGGCTAAGTA
CCGCGACGATTGGGAAAAATACGGCCTGTTCGACTTAGTTTCCACCGTCAATGAAGACGTGCTGCGCGCCTATTCGATGG
CGAACTATCCCGATGAAAAGGGCCGCATCATGCTTAACGTGCGGATTGCCACGCCACCATCGGCCAATGTGCCACCGGGT
AAGATGTCTTCTTACATCTTTAACCTCAAGGCGGGGGATAAGGTGACCATCTCTGGCCCATTCGGCGAGTTCTTTGTGAA
AGAAACCGATGCGGAAATGGTATTTATCGGCGGTGGTGCGGGTATGGCACCGATGCGCTCGCATATTTTCGATCAGCTAA
AGAGTAAAAAGACCAAGCGTAAGATGAGCTTCTGGTACGGCGCACGCTCAAGCCGCGAAGTGTTTTACCAGCAGGACTTC
GACACCTTGGCCGCCGAGAACGACAACTTCGTCTGGCATGTGGCGTTGTCTGAGCCGCTGCCAGAGGATAATTGGACGGG
TTACACTGGCTTTATTCATAATGTGCTCTATGAGAATTATCTTAAAAATCACAAGGCACCAGAGGATTGTGAGTTCTATA
TGTGTGGCCCACCGATCATGAACTCCTCGGTGATCCGTATGCTCGAAAGCCTAGGGGTCGAGCCTGAAAATATCCTGCTC
GATGACTTTGGTGATTAA

Upstream 100 bases:

>100_bases
AGGGTTGATGGCACTTGGCTTTATGTCTTTCTCGGGGATCTCGCTGTAACGCGTGGCTGAGAAAGTGAAACCGCATTCTG
TTTAGAAAAAGGGTTATCGA

Downstream 100 bases:

>100_bases
GCAGTAAGTGGAAAAGAAAATCGCGTTAGGCAGGGCTAGTACAGCTGAGTCTAACGCGATTTTTTTTTATAGGGCTTTTG
ATATCTAGCTAAAAGATTTT

Product: Na(+)-translocating NADH-quinone reductase subunit F

Products: @TETR01txt*tetrahydropteridine!; NO3-; NAD(P); Proton [C]

Alternate protein names: Na(+)-NQR subunit F; Na(+)-translocating NQR subunit F; NQR complex subunit F; NQR-1 subunit F [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MEMAIGIGMFTLVVCLLVIVILIAKKKLVTTGEITIGINDDAEKSIKVAAGDKLLGALASKNIFIPSACGGGGTCGQCRV
KVKSGGGDILPTEQGHITKKEAKEGCRLACQVAVKTDMELELEEEIFGVKKWQCEVISNDNKATFIKELLLKLPEGEDVH
FKAGGYIQIEAPAHVVKYADFDIPAKYRDDWEKYGLFDLVSTVNEDVLRAYSMANYPDEKGRIMLNVRIATPPSANVPPG
KMSSYIFNLKAGDKVTISGPFGEFFVKETDAEMVFIGGGAGMAPMRSHIFDQLKSKKTKRKMSFWYGARSSREVFYQQDF
DTLAAENDNFVWHVALSEPLPEDNWTGYTGFIHNVLYENYLKNHKAPEDCEFYMCGPPIMNSSVIRMLESLGVEPENILL
DDFGD

Sequences:

>Translated_405_residues
MEMAIGIGMFTLVVCLLVIVILIAKKKLVTTGEITIGINDDAEKSIKVAAGDKLLGALASKNIFIPSACGGGGTCGQCRV
KVKSGGGDILPTEQGHITKKEAKEGCRLACQVAVKTDMELELEEEIFGVKKWQCEVISNDNKATFIKELLLKLPEGEDVH
FKAGGYIQIEAPAHVVKYADFDIPAKYRDDWEKYGLFDLVSTVNEDVLRAYSMANYPDEKGRIMLNVRIATPPSANVPPG
KMSSYIFNLKAGDKVTISGPFGEFFVKETDAEMVFIGGGAGMAPMRSHIFDQLKSKKTKRKMSFWYGARSSREVFYQQDF
DTLAAENDNFVWHVALSEPLPEDNWTGYTGFIHNVLYENYLKNHKAPEDCEFYMCGPPIMNSSVIRMLESLGVEPENILL
DDFGD
>Mature_405_residues
MEMAIGIGMFTLVVCLLVIVILIAKKKLVTTGEITIGINDDAEKSIKVAAGDKLLGALASKNIFIPSACGGGGTCGQCRV
KVKSGGGDILPTEQGHITKKEAKEGCRLACQVAVKTDMELELEEEIFGVKKWQCEVISNDNKATFIKELLLKLPEGEDVH
FKAGGYIQIEAPAHVVKYADFDIPAKYRDDWEKYGLFDLVSTVNEDVLRAYSMANYPDEKGRIMLNVRIATPPSANVPPG
KMSSYIFNLKAGDKVTISGPFGEFFVKETDAEMVFIGGGAGMAPMRSHIFDQLKSKKTKRKMSFWYGARSSREVFYQQDF
DTLAAENDNFVWHVALSEPLPEDNWTGYTGFIHNVLYENYLKNHKAPEDCEFYMCGPPIMNSSVIRMLESLGVEPENILL
DDFGD

Specific function: NQR complex catalyzes the reduction of ubiquinone-1 to ubiquinol by two successive reactions, coupled with the transport of Na(+) ions from the cytoplasm to the periplasm. The first step is catalyzed by nqrF, which accepts electrons from NADH and reduces

COG id: COG2871

COG function: function code C; Na+-transporting NADH:ubiquinone oxidoreductase, subunit NqrF

Gene ontology:

Cell location: Cell inner membrane (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6323552, Length=200, Percent_Identity=27, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6323510, Length=154, Percent_Identity=25.974025974026, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322146, Length=191, Percent_Identity=27.7486910994764, Blast_Score=67, Evalue=5e-12,
Organism=Drosophila melanogaster, GI116007806, Length=256, Percent_Identity=26.171875, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI116007804, Length=256, Percent_Identity=26.171875, Blast_Score=69, Evalue=6e-12,
Organism=Drosophila melanogaster, GI281366101, Length=256, Percent_Identity=25.78125, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24662934, Length=256, Percent_Identity=25.78125, Blast_Score=66, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24662938, Length=256, Percent_Identity=25.78125, Blast_Score=66, Evalue=4e-11,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR010205
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.34 [C]

Molecular weight: Translated: 44848; Mature: 44848

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMAIGIGMFTLVVCLLVIVILIAKKKLVTTGEITIGINDDAEKSIKVAAGDKLLGALAS
CCEEEHHHHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCCCCEEEEECCHHHHHHHHC
KNIFIPSACGGGGTCGQCRVKVKSGGGDILPTEQGHITKKEAKEGCRLACQVAVKTDMEL
CCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEEEEECCCCE
ELEEEIFGVKKWQCEVISNDNKATFIKELLLKLPEGEDVHFKAGGYIQIEAPAHVVKYAD
EEHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCEEEEECC
FDIPAKYRDDWEKYGLFDLVSTVNEDVLRAYSMANYPDEKGRIMLNVRIATPPSANVPPG
CCCCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCC
KMSSYIFNLKAGDKVTISGPFGEFFVKETDAEMVFIGGGAGMAPMRSHIFDQLKSKKTKR
CCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KMSFWYGARSSREVFYQQDFDTLAAENDNFVWHVALSEPLPEDNWTGYTGFIHNVLYENY
HHHHHCCCCCCCCEEEECCCHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LKNHKAPEDCEFYMCGPPIMNSSVIRMLESLGVEPENILLDDFGD
HHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCC
>Mature Secondary Structure
MEMAIGIGMFTLVVCLLVIVILIAKKKLVTTGEITIGINDDAEKSIKVAAGDKLLGALAS
CCEEEHHHHHHHHHHHHHHHHHHCCCCEEEECEEEEEECCCCCCEEEEECCHHHHHHHHC
KNIFIPSACGGGGTCGQCRVKVKSGGGDILPTEQGHITKKEAKEGCRLACQVAVKTDMEL
CCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCHHHHHCCCEEEEEEEEECCCCE
ELEEEIFGVKKWQCEVISNDNKATFIKELLLKLPEGEDVHFKAGGYIQIEAPAHVVKYAD
EEHHHHHCCCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEECCCEEEEECCCEEEEECC
FDIPAKYRDDWEKYGLFDLVSTVNEDVLRAYSMANYPDEKGRIMLNVRIATPPSANVPPG
CCCCCHHHCCHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCCCC
KMSSYIFNLKAGDKVTISGPFGEFFVKETDAEMVFIGGGAGMAPMRSHIFDQLKSKKTKR
CCCCEEEEECCCCEEEEECCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
KMSFWYGARSSREVFYQQDFDTLAAENDNFVWHVALSEPLPEDNWTGYTGFIHNVLYENY
HHHHHCCCCCCCCEEEECCCHHHHCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHH
LKNHKAPEDCEFYMCGPPIMNSSVIRMLESLGVEPENILLDDFGD
HHHCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dihydropteridine; 2 nitric oxide; O2; NAD(P)H [C]

Specific reaction: NAD(P)H + dihydropteridine = NAD(P)+ @TETR01.txt*tetrahydropteridine! 2 nitric oxide + 2 O2 + NAD(P)H = 2 NO3- + NAD(P) + Proton [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10779868 [H]