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The map label for this gene is yaaJ [C]

Identifier: 113969058

GI number: 113969058

Start: 828281

End: 829639

Strand: Direct

Name: yaaJ [C]

Synonym: Shewmr4_0714

Alternate gene names: 113969058

Gene position: 828281-829639 (Clockwise)

Preceding gene: 113969044

Following gene: 113969059

Centisome position: 17.6

GC content: 52.61

Gene sequence:

>1359_bases
ATGAGTTTTCAAGCTTTACTCAGCAGCCTGAACGGTATCGTTTGGGGACCCGTTACCCTGTGTTTACTCGTGGGTACGGG
CATTTATCTCACCTTGCGTTTGCGGCTTATCCAAGTGTTTCGTCTGCCAATGGCACTCGGCCTATTGTTTAAACCTGCCA
AAGGGCATGGGGATTTATCTTCCTTCGCCGCCTTGTGCACGGCACTCTCCGCCACCATCGGTACGGGCAACATTGTCGGG
GTGGCAACCGCGATTAAGATGGGCGGCCCAGGGGCATTATTTTGGATGTGGCTGGCGGCCTTCTTTGGCATGGCGACCAA
ATATGCCGAATGTATGTTGGCGGTAAAATACCGCACTACCGATGCGCGCGGACAAATTGCGGGCGGCCCTATGTATTATA
TCGAGCGGGGTTTGGGCATACATTGGATGGCGAAGTTGTTTGCCCTGTTTGGTGTAGGGGTGGCATTTTTCGGTATTGGT
ACTTTCGCCCAAGTGAATGCGATTAGCGATGCCCTGACCATTGCCTTTGAGGTGCCCACTTGGGCCACGGCATCGGTGCT
CACCTTGTTAGTTGCGGCGGTGACCTTAGGTGGGGTTAAGCGTATTTCTAACGTGGCGCAAAAGTTAGTGCCAGCGATGT
CAATTGGTTATGTGCTGGCTTGTGTGTGGATTTTAGCGAGTTTTGCGGAATCGATTTTACCCGCCTTGCAATTAGTGGTG
GAGTCGGCCTTTACACCTGTCTCGGCGGCGGGGGGATTCTTAGGTGCAACTGTGGCGCAGGCGATTCAAATGGGGATTGC
CCGCGGGGTGTTTTCCAACGAGTCGGGCCTCGGTAGCGCCCCAATCGCTGCCGCGGCGGCAAAAACCAATGAGCCGGTCG
AGCAGGGTTTAGTCAGCATGACGGGCACCTTTTTCGATACCATCATCATCTGCACTATGACGGGACTGGTACTGATTATC
ACCGGAGTCTGGAGCGGTGATACTGCAGGCGCTGCCATGACCAGCGCGGCCTTCAGCCTAGGCGGCTCGGCAGTCGTTGG
TCAGTATTTAGTGACTATCGCCTTAGTCTGCTTTGCATTCACCACTATTTTAGGCTGGCACTACTATGGCGAGCGCTGCT
GGTACTATTTAGTGGGTGAGCGTGGGCTGCGTGCCTATCAAATCCTATTTTTAGGTTTGATTGCCGTGGGGGCTTTTATC
AAGCTGGATGTGATCTGGTTACTGGCCGATACGGTAAACGGTTTAATGGCGATACCTAACCTGATCGCGATAATTGGACT
TAGGCACGTTATCATCGCTGAAACTCAAAGTTATTTTGTTCGGGCACTCTCAAACCGTACACTAGACCCAATTTATTAA

Upstream 100 bases:

>100_bases
GGCTGGGTGATAGTCTTTCTTTAGGCTGGCAGCGGTGTGTCACAGCCATTTCTTTTCCTCCTCCTTTATTAATTTAGTTG
TTTAATAATTGAGGAATATC

Downstream 100 bases:

>100_bases
TGTTGTGAAAACAATATATTAGTTCACATTATTTTAACTTTGAGATTGTCGATACCGCGTTCTTCGTTTACTTTATAAGA
GCTACCCCAAAATGGGGAGA

Product: amino acid carrier protein

Products: Na (I) [Cytoplasm]; L-alanine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MSFQALLSSLNGIVWGPVTLCLLVGTGIYLTLRLRLIQVFRLPMALGLLFKPAKGHGDLSSFAALCTALSATIGTGNIVG
VATAIKMGGPGALFWMWLAAFFGMATKYAECMLAVKYRTTDARGQIAGGPMYYIERGLGIHWMAKLFALFGVGVAFFGIG
TFAQVNAISDALTIAFEVPTWATASVLTLLVAAVTLGGVKRISNVAQKLVPAMSIGYVLACVWILASFAESILPALQLVV
ESAFTPVSAAGGFLGATVAQAIQMGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSMTGTFFDTIIICTMTGLVLII
TGVWSGDTAGAAMTSAAFSLGGSAVVGQYLVTIALVCFAFTTILGWHYYGERCWYYLVGERGLRAYQILFLGLIAVGAFI
KLDVIWLLADTVNGLMAIPNLIAIIGLRHVIIAETQSYFVRALSNRTLDPIY

Sequences:

>Translated_452_residues
MSFQALLSSLNGIVWGPVTLCLLVGTGIYLTLRLRLIQVFRLPMALGLLFKPAKGHGDLSSFAALCTALSATIGTGNIVG
VATAIKMGGPGALFWMWLAAFFGMATKYAECMLAVKYRTTDARGQIAGGPMYYIERGLGIHWMAKLFALFGVGVAFFGIG
TFAQVNAISDALTIAFEVPTWATASVLTLLVAAVTLGGVKRISNVAQKLVPAMSIGYVLACVWILASFAESILPALQLVV
ESAFTPVSAAGGFLGATVAQAIQMGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSMTGTFFDTIIICTMTGLVLII
TGVWSGDTAGAAMTSAAFSLGGSAVVGQYLVTIALVCFAFTTILGWHYYGERCWYYLVGERGLRAYQILFLGLIAVGAFI
KLDVIWLLADTVNGLMAIPNLIAIIGLRHVIIAETQSYFVRALSNRTLDPIY
>Mature_451_residues
SFQALLSSLNGIVWGPVTLCLLVGTGIYLTLRLRLIQVFRLPMALGLLFKPAKGHGDLSSFAALCTALSATIGTGNIVGV
ATAIKMGGPGALFWMWLAAFFGMATKYAECMLAVKYRTTDARGQIAGGPMYYIERGLGIHWMAKLFALFGVGVAFFGIGT
FAQVNAISDALTIAFEVPTWATASVLTLLVAAVTLGGVKRISNVAQKLVPAMSIGYVLACVWILASFAESILPALQLVVE
SAFTPVSAAGGFLGATVAQAIQMGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSMTGTFFDTIIICTMTGLVLIIT
GVWSGDTAGAAMTSAAFSLGGSAVVGQYLVTIALVCFAFTTILGWHYYGERCWYYLVGERGLRAYQILFLGLIAVGAFIK
LDVIWLLADTVNGLMAIPNLIAIIGLRHVIIAETQSYFVRALSNRTLDPIY

Specific function: Unknown

COG id: COG1115

COG function: function code E; Na+/alanine symporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sodium:alanine (SAF) symporter family [H]

Homologues:

Organism=Escherichia coli, GI1786188, Length=468, Percent_Identity=36.965811965812, Blast_Score=243, Evalue=2e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001463 [H]

Pfam domain/function: PF01235 Na_Ala_symp [H]

EC number: NA

Molecular weight: Translated: 47636; Mature: 47505

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: PS00873 NA_ALANINE_SYMP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFQALLSSLNGIVWGPVTLCLLVGTGIYLTLRLRLIQVFRLPMALGLLFKPAKGHGDLS
CCHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SFAALCTALSATIGTGNIVGVATAIKMGGPGALFWMWLAAFFGMATKYAECMLAVKYRTT
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
DARGQIAGGPMYYIERGLGIHWMAKLFALFGVGVAFFGIGTFAQVNAISDALTIAFEVPT
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
WATASVLTLLVAAVTLGGVKRISNVAQKLVPAMSIGYVLACVWILASFAESILPALQLVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESAFTPVSAAGGFLGATVAQAIQMGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSM
HHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
TGTFFDTIIICTMTGLVLIITGVWSGDTAGAAMTSAAFSLGGSAVVGQYLVTIALVCFAF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TTILGWHYYGERCWYYLVGERGLRAYQILFLGLIAVGAFIKLDVIWLLADTVNGLMAIPN
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAIIGLRHVIIAETQSYFVRALSNRTLDPIY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SFQALLSSLNGIVWGPVTLCLLVGTGIYLTLRLRLIQVFRLPMALGLLFKPAKGHGDLS
CHHHHHHHCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHH
SFAALCTALSATIGTGNIVGVATAIKMGGPGALFWMWLAAFFGMATKYAECMLAVKYRTT
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
DARGQIAGGPMYYIERGLGIHWMAKLFALFGVGVAFFGIGTFAQVNAISDALTIAFEVPT
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCC
WATASVLTLLVAAVTLGGVKRISNVAQKLVPAMSIGYVLACVWILASFAESILPALQLVV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ESAFTPVSAAGGFLGATVAQAIQMGIARGVFSNESGLGSAPIAAAAAKTNEPVEQGLVSM
HHHCCCHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHH
TGTFFDTIIICTMTGLVLIITGVWSGDTAGAAMTSAAFSLGGSAVVGQYLVTIALVCFAF
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
TTILGWHYYGERCWYYLVGERGLRAYQILFLGLIAVGAFIKLDVIWLLADTVNGLMAIPN
HHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LIAIIGLRHVIIAETQSYFVRALSNRTLDPIY
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Periplasm]; L-alanine [Periplasm] [C]

Specific reaction: Na (I) [Periplasm] + L-alanine [Periplasm] = Na (I) [Cytoplasm] + L-alanine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]