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The map label for this gene is emrB [H]

Identifier: 113968868

GI number: 113968868

Start: 603446

End: 605053

Strand: Direct

Name: emrB [H]

Synonym: Shewmr4_0524

Alternate gene names: 113968868

Gene position: 603446-605053 (Clockwise)

Preceding gene: 113968867

Following gene: 113968869

Centisome position: 12.82

GC content: 51.93

Gene sequence:

>1608_bases
ATGAGCACAATTGCTGCGGCACCTGAAGCTATCGCCGCGGGGGCTACACCGAGCGAATATGAGCGTGGTAGTCGCCGTTC
GTGGATTGCTGTTTTCGGTGGTTTGATTGGCGCCTTTATGGCGATCCTCGATATTCAAATCACCAATGCCTCGATGAAGG
AAATCCAAGGAAGTCTCGGCGCTACGCTGGAGGAAGGCAGTTGGATTTCAACGGCTTATCTGGTTGCCGAGATGATTGCG
ATCCCGCTCTCGGGTTGGTTAAGCACAGGGCTTTCGGTCAGGCGTTATCTGTTGTGGACCACAGCTGCCTTTATCTTTGC
CTCTATTCTGTGTTCGATTTCGTGGAACCTCGAGGCCATGATCGCCTTTCGGGCGTTGCAGGGCTTCTTTGGTGGCGCGC
TGATCCCGCTGGCCTTTCGGTTGATCTTAGAATTTTTACCCGAGAATAAACGTGCCGTGGGGATGGCGCTCTTTGGCGTG
ACTGCGACGTTTGCGCCCTCGATTGGCCCAACCTTAGGTGGTTGGTTAACCGAGCATTTTAGTTGGCATTACCTGTTTTA
TATCAACGTGCCGCCCGGATTGCTGGTGATGGCAATGTTGGCCTATGGTCTCGAAAAACGCCCTGTGGTGTGGGATAAAC
TGAAGAATGCCGACTTAGCGGGCATTGTCACTATGGCGCTAGGCATGGGCTGCCTCGAGGTCGTGCTTGAGGAAGGTAAC
CGCAAGGATTGGTTTGGCTCGGATTTGATCCGCAATCTCGCCATCATTGCCGCGGTGAATTTAGTGCTGTTTGTGTGGAT
CCAGCTTAAACGTAAAGATCCTTTAGTGAATTTGCGCCTGCTCGGTAAACGCGATTTTGTGCTTTCGACCATAGCTTACT
TTTTGCTGGGGATGGCTCTCTTTGGCGCTATCTATTTGATCCCACTTTATTTGTCTCAAGTGCATGATTACACGCCGCTG
GAAATCGGCGAAGTCATTATGTGGATGGGGTTCCCGCAGCTCTTGGTATTGCCATTAGTGCCGCGTTTAATGCAACGTTT
CGACGGGCGTTATTTAGCCGCCTTCGGCTTTTTTATGTTTGCCCTCAGTTATTACATGAATAGCCAAATGACCGCCGATT
ACGCAGGGCCGCAGATGATCGCCTCCCAAGTGGTGCGCGCCTTAGGCCAGCCCTTTATCTTGGTGCCCATTGGCATGTTG
GCGACGGCCCATTTAAAGCCCCATGAGAATCCTTCGGCTTCGACCGTATTGAACGTGATGCGTAACTTAGGCGGTGCCTT
TGGTATTGCGCTGGTGGCGACCTTAAGCGATAACCTTGCCCGCGGTCATTTTGCCCATGTGAAGGAGTCTTTACCAGCGG
TCAGCACACAGGCGCAGGACTATTTGAATCAGAGCGCCAGTATGTTGCAACAGGGTGGCTCGGATCCCTATACCGCCTCG
ATGCAGGCCAAAGCACTATTAGGGCAAACCATGAGTCAGCAGGCCTATATTCAAGCCTATAACGATGTGTTTTTGATTAT
GGGGGCCATGTTGTTAATTGCCGTGGTGGCTGTGCTGCTTATCCGTAAACCTCAAGGGCCAGTCGCCCATGATGCGGCAA
TTGAATAA

Upstream 100 bases:

>100_bases
CGTGTCGTCCCCGGTTTAAGTGCCGTGGTGAAGGTCGATACTGCGTCGCATCCGACGACAGCTATCGCTGCTAACGTCGG
TCGCTAAGTCGGTCGTTAAC

Downstream 100 bases:

>100_bases
GACTGGTTTATACCAATCGTATTAAATATCTGTTCATTCAGCGGGAATTCAACGCGCTTTAGACAAGGCGAAGGCTTGAA
GGCATAGTGGTGCTCTGTCG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MSTIAAAPEAIAAGATPSEYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAEMIA
IPLSGWLSTGLSVRRYLLWTTAAFIFASILCSISWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPENKRAVGMALFGV
TATFAPSIGPTLGGWLTEHFSWHYLFYINVPPGLLVMAMLAYGLEKRPVVWDKLKNADLAGIVTMALGMGCLEVVLEEGN
RKDWFGSDLIRNLAIIAAVNLVLFVWIQLKRKDPLVNLRLLGKRDFVLSTIAYFLLGMALFGAIYLIPLYLSQVHDYTPL
EIGEVIMWMGFPQLLVLPLVPRLMQRFDGRYLAAFGFFMFALSYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGML
ATAHLKPHENPSASTVLNVMRNLGGAFGIALVATLSDNLARGHFAHVKESLPAVSTQAQDYLNQSASMLQQGGSDPYTAS
MQAKALLGQTMSQQAYIQAYNDVFLIMGAMLLIAVVAVLLIRKPQGPVAHDAAIE

Sequences:

>Translated_535_residues
MSTIAAAPEAIAAGATPSEYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAEMIA
IPLSGWLSTGLSVRRYLLWTTAAFIFASILCSISWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPENKRAVGMALFGV
TATFAPSIGPTLGGWLTEHFSWHYLFYINVPPGLLVMAMLAYGLEKRPVVWDKLKNADLAGIVTMALGMGCLEVVLEEGN
RKDWFGSDLIRNLAIIAAVNLVLFVWIQLKRKDPLVNLRLLGKRDFVLSTIAYFLLGMALFGAIYLIPLYLSQVHDYTPL
EIGEVIMWMGFPQLLVLPLVPRLMQRFDGRYLAAFGFFMFALSYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGML
ATAHLKPHENPSASTVLNVMRNLGGAFGIALVATLSDNLARGHFAHVKESLPAVSTQAQDYLNQSASMLQQGGSDPYTAS
MQAKALLGQTMSQQAYIQAYNDVFLIMGAMLLIAVVAVLLIRKPQGPVAHDAAIE
>Mature_534_residues
STIAAAPEAIAAGATPSEYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLGATLEEGSWISTAYLVAEMIAI
PLSGWLSTGLSVRRYLLWTTAAFIFASILCSISWNLEAMIAFRALQGFFGGALIPLAFRLILEFLPENKRAVGMALFGVT
ATFAPSIGPTLGGWLTEHFSWHYLFYINVPPGLLVMAMLAYGLEKRPVVWDKLKNADLAGIVTMALGMGCLEVVLEEGNR
KDWFGSDLIRNLAIIAAVNLVLFVWIQLKRKDPLVNLRLLGKRDFVLSTIAYFLLGMALFGAIYLIPLYLSQVHDYTPLE
IGEVIMWMGFPQLLVLPLVPRLMQRFDGRYLAAFGFFMFALSYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGMLA
TAHLKPHENPSASTVLNVMRNLGGAFGIALVATLSDNLARGHFAHVKESLPAVSTQAQDYLNQSASMLQQGGSDPYTASM
QAKALLGQTMSQQAYIQAYNDVFLIMGAMLLIAVVAVLLIRKPQGPVAHDAAIE

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=497, Percent_Identity=30.784708249497, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788710, Length=514, Percent_Identity=29.9610894941634, Blast_Score=223, Evalue=3e-59,
Organism=Escherichia coli, GI1790195, Length=330, Percent_Identity=25.4545454545455, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI87081983, Length=407, Percent_Identity=26.044226044226, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1788392, Length=328, Percent_Identity=25.9146341463415, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6325455, Length=135, Percent_Identity=28.8888888888889, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=24.6666666666667, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58364; Mature: 58232

Theoretical pI: Translated: 7.27; Mature: 7.27

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTIAAAPEAIAAGATPSEYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLG
CCCCCCCCHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
ATLEEGSWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASILCSISWNLEAM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IAFRALQGFFGGALIPLAFRLILEFLPENKRAVGMALFGVTATFAPSIGPTLGGWLTEHF
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
SWHYLFYINVPPGLLVMAMLAYGLEKRPVVWDKLKNADLAGIVTMALGMGCLEVVLEEGN
CEEEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
RKDWFGSDLIRNLAIIAAVNLVLFVWIQLKRKDPLVNLRLLGKRDFVLSTIAYFLLGMAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
FGAIYLIPLYLSQVHDYTPLEIGEVIMWMGFPQLLVLPLVPRLMQRFDGRYLAAFGFFMF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALSYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGMLATAHLKPHENPSASTVLNVM
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHH
RNLGGAFGIALVATLSDNLARGHFAHVKESLPAVSTQAQDYLNQSASMLQQGGSDPYTAS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MQAKALLGQTMSQQAYIQAYNDVFLIMGAMLLIAVVAVLLIRKPQGPVAHDAAIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure 
STIAAAPEAIAAGATPSEYERGSRRSWIAVFGGLIGAFMAILDIQITNASMKEIQGSLG
CCCCCCCHHHHCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHC
ATLEEGSWISTAYLVAEMIAIPLSGWLSTGLSVRRYLLWTTAAFIFASILCSISWNLEAM
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
IAFRALQGFFGGALIPLAFRLILEFLPENKRAVGMALFGVTATFAPSIGPTLGGWLTEHF
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCC
SWHYLFYINVPPGLLVMAMLAYGLEKRPVVWDKLKNADLAGIVTMALGMGCLEVVLEEGN
CEEEEEEEECCHHHHHHHHHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCC
RKDWFGSDLIRNLAIIAAVNLVLFVWIQLKRKDPLVNLRLLGKRDFVLSTIAYFLLGMAL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHH
FGAIYLIPLYLSQVHDYTPLEIGEVIMWMGFPQLLVLPLVPRLMQRFDGRYLAAFGFFMF
HHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
ALSYYMNSQMTADYAGPQMIASQVVRALGQPFILVPIGMLATAHLKPHENPSASTVLNVM
HHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCEEEEEHHHHHHHCCCCCCCCCHHHHHHHH
RNLGGAFGIALVATLSDNLARGHFAHVKESLPAVSTQAQDYLNQSASMLQQGGSDPYTAS
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCHH
MQAKALLGQTMSQQAYIQAYNDVFLIMGAMLLIAVVAVLLIRKPQGPVAHDAAIE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]