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The map label for this gene is acnB [H]

Identifier: 113968781

GI number: 113968781

Start: 493650

End: 496322

Strand: Direct

Name: acnB [H]

Synonym: Shewmr4_0437

Alternate gene names: 113968781

Gene position: 493650-496322 (Clockwise)

Preceding gene: 113968777

Following gene: 113968783

Centisome position: 10.49

GC content: 48.9

Gene sequence:

>2673_bases
ATGTCGGACTGTCAGCTTCAATTGAGAAGACGTGCAGTTTCGTTGTTAGAGCGCCAAACAAAGAGGAATGTTGCCGTGCT
AGAAGCATATCGTAAACACGTCGCAGAACGTGCTGCAGAGGGCGTAGTCCCTAAGCCATTAGATGCACATCAAGTGGCTG
AACTGGTTAAATTGGTTCAAAATCCCCCCGCTGGTGAAGAAGCCGTTATTCTTGACCTGCTAGAAAATCGAATTCCCCCC
GGTGTTGACGAAGCTGCTTACGTAAAAGCCGCTTTCTTAGATGCGGTTGCTAAAGGCACTGCAACTTCACCTATCCTGTC
TGCCGAACGTGCGACTGAGTTATTAGGCACCATGCAAGGTGGCTATAACATCGAGCCGCTGATCGCTCAGTTAGATAATC
CAGCTCTGGCACCATTAGCCGTTAAAGCACTGTCTCACACTCTGCTGATGTTTGATTCTTTCCACGATGTGGTTGAGAAA
ATGGAAGCGGGTAACGCCTATGCTAAACAAGTGGTTGAAGCTTGGGCAAATGCAGACTGGTACTTAAGCCGTCCAAAATT
AGCGGACAAAGTGACTCTGACAGTATTCAAAGTCTCAGGTGAAACCAACACCGACGACCTGTCACCGGCTCCTGATGCTT
GGTCACGTCCAGATATCCCGTTGCACGCCTTAGCTATGCTGAAAAACGCGCGTGATGGTATCGAGCCAGATGTACCAGGC
AGCGTAGGTCCAATCAAGAAATTAGAAGAACTGAAAACCAAAGGTTTCCCATTAGTTTACGTGGGTGACGTTGTAGGTAC
AGGCTCATCACGTAAGTCAGCGACTAACTCAGTACTGTGGTTCATGGGCGATGATATCCCATTCGTACCTAACAAACGTG
CTGGTGGTTTCTGCTTAGGTGGCAAGATTGCTCCGATTTTCTTCAACACTATGGAAGATGCGGGCGCGCTGCCAATCGAA
CTTGACGTCAGCAAAATGGAAATGGGCGATGTAATCGACATTTATCCATACGCTGGTGTTGTTAAGCGTCATGGCACAGA
GGAAGTCATTTCTGAGTTCAGCCTGAAAACCGACGTATTGTTAGACGAAGTGCGTGCGGGTGGCCGTATTCCACTGATCA
TCGGTCGTGGTTTAACTGACAAAGCTCGCGCAGTATTAGGTTTACCTGCTTCTGAAGTATTCGTACGTCCACAGGATATC
GCTGATTCTGGCAAAGGTTACACCCTTGCGCAGAAGATGGTTGGTAAAGCCTGTGGTGTTGCTGGCGTGCGTCCAGGCCA
ATACTGCGAACCTAAGATGACGTCTGTCGGTTCACAAGATACTACAGGTCCTATGACCCGTGATGAGCTGAAAGACTTAG
CCTGTTTAGGCTTCAGTGCCGATTTGACTATGCAGTCATTCTGCCACACTGCGGCTTATCCAAAACCAGTTGACGTTAAC
ACTCACCACACTCTGCCAGACTTCATCATGAACCGTGGCGGTGTGTCTCTGCGTCCAGGTGACGGTGTTATCCACTCTTG
GTTAAACCGTATGTTATTACCAGACACAGTTGGTACTGGTGGTGACTCACATACGCGTTTCCCAATCGGGATTTCATTCC
CAGCAGGTTCTGGTTTAGTGGCATTCGCTGCCGCGACCGGTGTGATGCCTCTGGACATGCCAGAATCAGTATTAGTGCGC
TTCAAGGGCAAGATGCAACCTGGCATCACCCTACGTGACTTAGTACACGCGATTCCATTAAAAGCGATTGAAATGGGTCT
GCTGACCGTTGAGAAGAAAGGTAAAGTGAACATCTTCTCTGGCCGCGTACTGGAAATCGAAGGCTTAGAAACCCTGAAAG
TAGAACAGGCATTCGAACTGTCTGACGCTTCTGCAGAACGCTCTGCAGCGGGTTGTACTATCAAGTTAGACAAAGAGCCA
ATCATCGAGTACCTGAACTCGAACATCACTATGCTGAAGTGGATGATCGCCGAAGGTTACGGTGACCGTCGTACTATCGA
ACGTCGTATCAAAGGCATGGAAGAGTGGTTAGCGAATCCTGAGCTGATGAGCGCAGACGCCGATGCCGAGTACGCTGCGG
TGATCGAAATCGATCTGAACGAGATCAAAGAGCCAATCCTGTGTGCGCCAAACGATCCAGACGATGCAGTATTGCTGTCA
TCTGTTGCGCAAACTAAGATTGACGAAGTGTTCGTGGGTTCTTGTATGACTAACATCGGTCACTTCCGTGCGACCGGTAA
GATGTTAGACAAGTTCGCTAAGACGCTGCCAACACGTCTGTGGATTGCACCACCGACGAAGATGGACAAAGACCAGCTGA
CCGAAGAAGGTTACTACGGTATTTTCGGTCGCGTTGGTGCGCGTATCGAGATCCCAGGCTGTTCACTGTGTATGGGTAAC
CAAGCACGTGTGGCTGAAGGTTCTACTGTGGTATCGACTTCTACCCGTAACTTCCCGAACCGTTTAGGTACAGGCGCTAA
CGTTTACTTAGCTTCTGCGGAATTAGCGGCAGTAGCAGCCCTGTTAGGTCGTCTGCCATCGGTTGAAGAATACCAAGAAT
ACGCTAAAGAGTTAGATGCAACTGCAGCTGACACTTACCGTTACTTGAACTTCGATCAGATCGATTCTTACACTCAGAAA
GCATCACAAGTGATCTTTCAATCAGCTGTATAA

Upstream 100 bases:

>100_bases
ACCTCCGGATTTAGCCCTTATTTCGTCTTTCGCCGAGTGTGACCTGATTCATACTTTTGCAAAGCTGTAGTATACTGCTG
GCCGGAAATACGGGTCGATC

Downstream 100 bases:

>100_bases
TCGATTGATATGATAATGAAAAAGCCAGCGAAAGCTGGCTTTTTTGTTTCTCTATTTTACCCGTTGTTATTCTGCTTATT
AAGACTGCGTAATCGAGTTA

Product: bifunctional aconitate hydratase 2/2-methylisocitrate dehydratase

Products: NA

Alternate protein names: Aconitase; 2-methylisocitrate dehydratase; Citrate hydro-lyase [H]

Number of amino acids: Translated: 890; Mature: 889

Protein sequence:

>890_residues
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAVILDLLENRIPP
GVDEAAYVKAAFLDAVAKGTATSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEK
MEAGNAYAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPG
SVGPIKKLEELKTKGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIE
LDVSKMEMGDVIDIYPYAGVVKRHGTEEVISEFSLKTDVLLDEVRAGGRIPLIIGRGLTDKARAVLGLPASEVFVRPQDI
ADSGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
FKGKMQPGITLRDLVHAIPLKAIEMGLLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEP
IIEYLNSNITMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADADAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLS
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGN
QARVAEGSTVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSVEEYQEYAKELDATAADTYRYLNFDQIDSYTQK
ASQVIFQSAV

Sequences:

>Translated_890_residues
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAVILDLLENRIPP
GVDEAAYVKAAFLDAVAKGTATSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEK
MEAGNAYAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPG
SVGPIKKLEELKTKGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIE
LDVSKMEMGDVIDIYPYAGVVKRHGTEEVISEFSLKTDVLLDEVRAGGRIPLIIGRGLTDKARAVLGLPASEVFVRPQDI
ADSGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVR
FKGKMQPGITLRDLVHAIPLKAIEMGLLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEP
IIEYLNSNITMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADADAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLS
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGN
QARVAEGSTVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSVEEYQEYAKELDATAADTYRYLNFDQIDSYTQK
ASQVIFQSAV
>Mature_889_residues
SDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQNPPAGEEAVILDLLENRIPPG
VDEAAYVKAAFLDAVAKGTATSPILSAERATELLGTMQGGYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKM
EAGNAYAKQVVEAWANADWYLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPGS
VGPIKKLEELKTKGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLGGKIAPIFFNTMEDAGALPIEL
DVSKMEMGDVIDIYPYAGVVKRHGTEEVISEFSLKTDVLLDEVRAGGRIPLIIGRGLTDKARAVLGLPASEVFVRPQDIA
DSGKGYTLAQKMVGKACGVAGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVNT
HHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLVAFAAATGVMPLDMPESVLVRF
KGKMQPGITLRDLVHAIPLKAIEMGLLTVEKKGKVNIFSGRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPI
IEYLNSNITMLKWMIAEGYGDRRTIERRIKGMEEWLANPELMSADADAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLSS
VAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDKDQLTEEGYYGIFGRVGARIEIPGCSLCMGNQ
ARVAEGSTVVSTSTRNFPNRLGTGANVYLASAELAAVAALLGRLPSVEEYQEYAKELDATAADTYRYLNFDQIDSYTQKA
SQVIFQSAV

Specific function: Catalyzes the isomerization of citrate to isocitrate via cis-aconitate as well as the dehydration of 2-methylisocitrate to cis-2-methylaconitate [H]

COG id: COG1049

COG function: function code C; Aconitase B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family [H]

Homologues:

Organism=Escherichia coli, GI2367097, Length=865, Percent_Identity=81.1560693641619, Blast_Score=1439, Evalue=0.0,
Organism=Escherichia coli, GI1786259, Length=389, Percent_Identity=25.706940874036, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6323335, Length=381, Percent_Identity=24.9343832020997, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015928
- InterPro:   IPR018136
- InterPro:   IPR004406
- InterPro:   IPR015930
- InterPro:   IPR015933
- InterPro:   IPR015929 [H]

Pfam domain/function: PF00330 Aconitase; PF06434 Aconitase_2_N; PF11791 Aconitase_B_N [H]

EC number: =4.2.1.3; =4.2.1.99 [H]

Molecular weight: Translated: 96299; Mature: 96167

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQ
CCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPPAGEEAVILDLLENRIPPGVDEAAYVKAAFLDAVAKGTATSPILSAERATELLGTMQG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
GYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKMEAGNAYAKQVVEAWANADW
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCE
YLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPG
EECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
SVGPIKKLEELKTKGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLG
CCCHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEC
GKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYAGVVKRHGTEEVISEFSLKTDVL
CEEEEHEEECHHCCCCCEEEEEHHHHCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHH
LDEVRAGGRIPLIIGRGLTDKARAVLGLPASEVFVRPQDIADSGKGYTLAQKMVGKACGV
HHHHHCCCCEEEEEECCCCHHHHHEECCCHHHHEECCHHHCCCCCCHHHHHHHHHHHHCC
AGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
CCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLV
CCCCCHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCE
AFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLKAIEMGLLTVEKKGKVNIFS
EHHHHCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEC
GRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGY
CEEEEECCCCEEEHHHHHCCCCCCCHHCCCCCEEEECHHHHHHHHCCCHHEEHHHHHCCC
GDRRTIERRIKGMEEWLANPELMSADADAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHH
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDKDQLTEEGYYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCCC
IFGRVGARIEIPGCSLCMGNQARVAEGSTVVSTSTRNFPNRLGTGANVYLASAELAAVAA
HHHCCCCEEECCCCCEECCCCCEECCCCEEEEECCCCCHHHHCCCCEEEEEHHHHHHHHH
LLGRLPSVEEYQEYAKELDATAADTYRYLNFDQIDSYTQKASQVIFQSAV
HHHCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SDCQLQLRRRAVSLLERQTKRNVAVLEAYRKHVAERAAEGVVPKPLDAHQVAELVKLVQ
CCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHC
NPPAGEEAVILDLLENRIPPGVDEAAYVKAAFLDAVAKGTATSPILSAERATELLGTMQG
CCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCC
GYNIEPLIAQLDNPALAPLAVKALSHTLLMFDSFHDVVEKMEAGNAYAKQVVEAWANADW
CCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCE
YLSRPKLADKVTLTVFKVSGETNTDDLSPAPDAWSRPDIPLHALAMLKNARDGIEPDVPG
EECCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCC
SVGPIKKLEELKTKGFPLVYVGDVVGTGSSRKSATNSVLWFMGDDIPFVPNKRAGGFCLG
CCCHHHHHHHHHHCCCCEEEECCEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCEEEC
GKIAPIFFNTMEDAGALPIELDVSKMEMGDVIDIYPYAGVVKRHGTEEVISEFSLKTDVL
CEEEEHEEECHHCCCCCEEEEEHHHHCCCCEEEECCCHHHHHHCCHHHHHHHHHHHHHHH
LDEVRAGGRIPLIIGRGLTDKARAVLGLPASEVFVRPQDIADSGKGYTLAQKMVGKACGV
HHHHHCCCCEEEEEECCCCHHHHHEECCCHHHHEECCHHHCCCCCCHHHHHHHHHHHHCC
AGVRPGQYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLTMQSFCHTAAYPKPVDVN
CCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCC
THHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISFPAGSGLV
CCCCCHHHHHCCCCCEECCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCE
AFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLKAIEMGLLTVEKKGKVNIFS
EHHHHCCCCCCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEC
GRVLEIEGLETLKVEQAFELSDASAERSAAGCTIKLDKEPIIEYLNSNITMLKWMIAEGY
CEEEEECCCCEEEHHHHHCCCCCCCHHCCCCCEEEECHHHHHHHHCCCHHEEHHHHHCCC
GDRRTIERRIKGMEEWLANPELMSADADAEYAAVIEIDLNEIKEPILCAPNDPDDAVLLS
CCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEHHHCCCCEEECCCCCCHHHHHH
SVAQTKIDEVFVGSCMTNIGHFRATGKMLDKFAKTLPTRLWIAPPTKMDKDQLTEEGYYG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCHHHHCCCCCCC
IFGRVGARIEIPGCSLCMGNQARVAEGSTVVSTSTRNFPNRLGTGANVYLASAELAAVAA
HHHCCCCEEECCCCCEECCCCCEECCCCEEEEECCCCCHHHHCCCCEEEEEHHHHHHHHH
LLGRLPSVEEYQEYAKELDATAADTYRYLNFDQIDSYTQKASQVIFQSAV
HHHCCCCHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8202364; 9278503; 8932712; 9298646; 10585860; 8000525; 11992126 [H]