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The map label for this gene is oprF [H]

Identifier: 113968495

GI number: 113968495

Start: 170060

End: 171523

Strand: Direct

Name: oprF [H]

Synonym: Shewmr4_0150

Alternate gene names: 113968495

Gene position: 170060-171523 (Clockwise)

Preceding gene: 113968493

Following gene: 113968500

Centisome position: 3.61

GC content: 47.2

Gene sequence:

>1464_bases
ATGCGTGTTTCTAAGCGGAGTTGTTTTGCGGGGTTATTGGCTATGTCGACGAGCCTGTTGCCATTGTCGTTGATGGCGGC
GGATGAGGGATTATTTACGCCTTTTCCTACGGCGAAATTAAAGGATGAATTGACGCGCCATTATGCCGAATATCCCTTTA
TTATCAGCGCGGGTGAACAAAGATCTGACTATCGTTTTAAGCCTATCTTCGGTCAGGTCCATCAGCAAAAATATAAATTA
GCGCCTGAATATAGCCCCGCGCATATTCTCGACAATTACCGTGAGCAGATTAAAAAGCTTGGTGGCGAAGTCCTATTCGA
GTGTCTGCTTGAGGCCTGTGGTGATTCTCGCTTATTGCGAGAGAAAATTGCCCTGTTAGATGGTGTTATTGGTGTTGAAA
GTGGTTACTTGTTTGCTCGAGTTAAAAATGAGCAAAAAGAGTTTTATACCGCCATACAAGCAGCAGTTATGGATGATTCT
GCGGGTCTTCATATTACGACGCTCGAGGTGATCCCCGAGCCGTTAGATTTAGTCACAGTTAATGCTGCATTTTTACAGCA
GGCGCCTAAGCAAATCGAGTTTAAAGATAATCAAAAGAAGGATACCAACGGCGCCGAGGATCATCCGCTTATCAGCCGAC
TAAAGGGAAGTTATATCCAGGACTTTAAACGCCAAGGCTTTGCCAGCACTGCCTTTGTGTCGTCAATGGATGCAAGCAAT
AAGCCTGTGCTGGTGGAGCGTGCGGGTAAGTTGACTCAAAATGGCTATTCAATACCAAAGGAATATTCGGGTTACGAGGT
GTTCACTAACTACAAGGTGGCATTGGCTAAACTCGGATTCGAGACGCAATTTGAGTGTCTGGGAAAAGCCTGTGGTAGGG
AGGATAGGTTTATCAAAGCCATTGATTCTCTGGTGAACATTGGTAACGAAGACAGCCAGTTTTATGCTTGGTATACCTTA
ACGCGCCCCGAGGGGAATGTGCATGTGAGCACTTATACCCTTGGTTATATTGATGGCTTATGGACCGACTTGCGGGTGTT
AGAGGAGACTGAGCTTAAGGATGATCGCGTGGGGATCAATCTTGAGGCGCTGACCGACCAAATCGAAGCCAAGGGGCATG
TTGCCTTAGAAGGGCTGTTATTCGAGTACGATAGCGACAAGTTGTTGCCCGAGGCGCAGCCCGTTGTCGAGGTGCTTGCC
AGTTATTTAAAGGCAAATCCTAAGCGTGCATTTTATGTGGTGGGTCATACCGATGACAAAGGTAATCAAGCCTATAACCA
AGGTCTATCCCAGCGCCGCGCGGTGGCGATTGTGAAGTTATTGACCACGAGTCATGGTATTCCAACTCAACAATTGGAAG
CCGTAGGCATAGGTGAACATGCTCCCGTTGCTAGCAACAGTGATGAGGCGGGGCAGCAGCAGAATCGCCGTGTCGAGTTA
GTATTGAGATCGGATACGAAGTAA

Upstream 100 bases:

>100_bases
CCTCAATCACGCTAAAGTATTAACTTGTTTCATATTTGTTATTGCGCAATCCGTTTAGTGTTACTTTTCGCAGCAGTATT
TCTATGGAGAGTAAATATTG

Downstream 100 bases:

>100_bases
ACCCAATCGTTTGATTCAATAGATATAAAAATGGCGTCAACTCTTGTCGAGTTGGCGCCATTTTTGTTTATCTGTGATAG
TTCAGCCGACGTCTCTGCCA

Product: OmpA/MotB domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MRVSKRSCFAGLLAMSTSLLPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQRSDYRFKPIFGQVHQQKYKL
APEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLREKIALLDGVIGVESGYLFARVKNEQKEFYTAIQAAVMDDS
AGLHITTLEVIPEPLDLVTVNAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALAKLGFETQFECLGKACGREDRFIKAIDSLVNIGNEDSQFYAWYTL
TRPEGNVHVSTYTLGYIDGLWTDLRVLEETELKDDRVGINLEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLA
SYLKANPKRAFYVVGHTDDKGNQAYNQGLSQRRAVAIVKLLTTSHGIPTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
VLRSDTK

Sequences:

>Translated_487_residues
MRVSKRSCFAGLLAMSTSLLPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQRSDYRFKPIFGQVHQQKYKL
APEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLREKIALLDGVIGVESGYLFARVKNEQKEFYTAIQAAVMDDS
AGLHITTLEVIPEPLDLVTVNAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALAKLGFETQFECLGKACGREDRFIKAIDSLVNIGNEDSQFYAWYTL
TRPEGNVHVSTYTLGYIDGLWTDLRVLEETELKDDRVGINLEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLA
SYLKANPKRAFYVVGHTDDKGNQAYNQGLSQRRAVAIVKLLTTSHGIPTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
VLRSDTK
>Mature_487_residues
MRVSKRSCFAGLLAMSTSLLPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQRSDYRFKPIFGQVHQQKYKL
APEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLREKIALLDGVIGVESGYLFARVKNEQKEFYTAIQAAVMDDS
AGLHITTLEVIPEPLDLVTVNAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALAKLGFETQFECLGKACGREDRFIKAIDSLVNIGNEDSQFYAWYTL
TRPEGNVHVSTYTLGYIDGLWTDLRVLEETELKDDRVGINLEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLA
SYLKANPKRAFYVVGHTDDKGNQAYNQGLSQRRAVAIVKLLTTSHGIPTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
VLRSDTK

Specific function: Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium [H]

COG id: COG2885

COG function: function code M; Outer membrane protein and related peptidoglycan-associated (lipo)proteins

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OmpA-like domain [H]

Homologues:

Organism=Escherichia coli, GI1788957, Length=108, Percent_Identity=34.2592592592593, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI48994946, Length=104, Percent_Identity=37.5, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1787191, Length=117, Percent_Identity=38.4615384615385, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011250
- InterPro:   IPR006664
- InterPro:   IPR006690
- InterPro:   IPR006665
- InterPro:   IPR008722 [H]

Pfam domain/function: PF00691 OmpA; PF05736 OprF [H]

EC number: NA

Molecular weight: Translated: 54325; Mature: 54325

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS01068 OMPA_1 ; PS51123 OMPA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVSKRSCFAGLLAMSTSLLPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQ
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
RSDYRFKPIFGQVHQQKYKLAPEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLR
CCCCEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EKIALLDGVIGVESGYLFARVKNEQKEFYTAIQAAVMDDSAGLHITTLEVIPEPLDLVTV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCEEEE
NAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
CHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCC
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALAKLGFETQFECLGKACGREDRFIKA
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCEEHEHHCCCCHHHHHHHHHCCCCHHHHHH
IDSLVNIGNEDSQFYAWYTLTRPEGNVHVSTYTLGYIDGLWTDLRVLEETELKDDRVGIN
HHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
LEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLASYLKANPKRAFYVVGHTDDK
HHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GNQAYNQGLSQRRAVAIVKLLTTSHGIPTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCCCCCHHHHHCCCEEEE
VLRSDTK
EEECCCC
>Mature Secondary Structure
MRVSKRSCFAGLLAMSTSLLPLSLMAADEGLFTPFPTAKLKDELTRHYAEYPFIISAGEQ
CCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCEEEECCCC
RSDYRFKPIFGQVHQQKYKLAPEYSPAHILDNYREQIKKLGGEVLFECLLEACGDSRLLR
CCCCEECCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
EKIALLDGVIGVESGYLFARVKNEQKEFYTAIQAAVMDDSAGLHITTLEVIPEPLDLVTV
HHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCEEEEEHHHCCCCCCEEEE
NAAFLQQAPKQIEFKDNQKKDTNGAEDHPLISRLKGSYIQDFKRQGFASTAFVSSMDASN
CHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHHHHHHHCCCHHHHHHHHCCCCC
KPVLVERAGKLTQNGYSIPKEYSGYEVFTNYKVALAKLGFETQFECLGKACGREDRFIKA
CCEEEECCCCCCCCCCCCCCCCCCEEEEECCEEHEHHCCCCHHHHHHHHHCCCCHHHHHH
IDSLVNIGNEDSQFYAWYTLTRPEGNVHVSTYTLGYIDGLWTDLRVLEETELKDDRVGIN
HHHHHHCCCCCCCEEEEEEEECCCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCC
LEALTDQIEAKGHVALEGLLFEYDSDKLLPEAQPVVEVLASYLKANPKRAFYVVGHTDDK
HHHHHHHHHCCCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCC
GNQAYNQGLSQRRAVAIVKLLTTSHGIPTQQLEAVGIGEHAPVASNSDEAGQQQNRRVEL
CCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHEECCCCCCCCCCCCCHHHHHCCCEEEE
VLRSDTK
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1898935 [H]